prEN ISO 10565
(Main)Oilseeds - Simultaneous determination of oil and water contents - Method using pulsed nuclear magnetic resonance spectrometry (ISO/DIS 10565:2025)
Oilseeds - Simultaneous determination of oil and water contents - Method using pulsed nuclear magnetic resonance spectrometry (ISO/DIS 10565:2025)
This International Standard specifies a rapid method for the determination of the oil and water contents of commercial oilseeds using pulsed nuclear magnetic resonance (NMR). It is applicable to rapeseeds, soya beans, linseeds and sunflower seeds with a water content less than 10 %. For seeds with higher water contents, drying is necessary before the oil content can be determined by pulsed NMR. NOTE 1 This method has been tested with rapeseeds, soya beans, linseeds and sunflower seeds. This does not, however, preclude its applicability to other commercial seeds whose oil is liquid at the temperature of measurement. NOTE 2 The reproducibility values are generally higher than those obtained by the drying method (ISO 665)
Ölsaaten - Gleichzeitige Bestimmung des Öl- und Wassergehalts - Verfahren mit gepulster Kernspinresonanzspektrometrie (ISO/DIS 10565:2025)
Graines oléagineuses - Détermination simultanée de la teneur en huile et en eau - Méthode par spectrométrie par résonance magnétique nucléaire pulsée (ISO/DIS 10565:2025)
La présente Norme internationale spécifie une méthode de détermination rapide de la teneur en huile et en eau dans les graines entières du commerce, par spectrométrie par résonance magnétique nucléaire (RMN) pulsée. Elle est applicable aux graines de colza, de soja, de lin et de tournesol dont la teneur en eau est inférieure à 10 %. Pour des graines ayant des teneurs en eau plus élevées, il est nécessaire de les sécher avant de déterminer leur teneur en huile par RMN pulsée. NOTE 1 Cette méthode a été expérimentée sur les graines de colza, de soja, de lin et de tournesol. Cela n'exclut toutefois pas qu'elle puisse s'appliquer également à d'autres graines oléagineuses du commerce dont l'huile est liquide à la température de mesurage. NOTE 2 Les valeurs de reproductibilité obtenues sont généralement plus élevées que celles obtenues par la méthode par dessiccation (ISO 665).
Oljna semena - Simultano določanje olja in vode - Spektrometrija jedrske magnetne resonance s pulznim delovanjem (ISO/DIS 10565:2025)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2025
Oljna semena - Simultano določevanje olja in vode - Spektrometrijska metoda z
uporabo pulzne jedrske magnetne resonance (ISO/DIS 10565:2025)
Oilseeds - Simultaneous determination of oil and water contents - Method using pulsed
nuclear magnetic resonance spectrometry (ISO/DIS 10565:2025)
Ölsaaten - Gleichzeitige Bestimmung des Öl- und Wassergehalts - Verfahren mit
gepulster Kernspinresonanzspektrometrie (ISO/DIS 10565:2025)
Graines oléagineuses - Détermination simultanée de la teneur en huile et en eau -
Méthode par spectrométrie par résonance magnétique nucléaire pulsée (ISO/DIS
10565:2025)
Ta slovenski standard je istoveten z: prEN ISO 10565
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
DRAFT
International
Standard
ISO/DIS 10565
ISO/TC 34/SC 2
Oilseeds — Simultaneous
Secretariat: AFNOR
determination of oil and water
Voting begins on:
contents — Method using pulsed
2025-05-27
nuclear magnetic resonance
Voting terminates on:
spectrometry
2025-08-19
Graines oléagineuses — Détermination simultanée de la teneur
en huile et en eau — Méthode par spectrométrie par résonance
magnétique nucléaire pulsée
ICS: 67.200.20
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference number
ISO/DIS 10565:2025(en)
DRAFT
ISO/DIS 10565:2025(en)
International
Standard
ISO/DIS 10565
ISO/TC 34/SC 2
Oilseeds — Simultaneous
Secretariat: AFNOR
determination of oil and water
Voting begins on:
contents — Method using pulsed
nuclear magnetic resonance
Voting terminates on:
spectrometry
Graines oléagineuses — Détermination simultanée de la teneur
en huile et en eau — Méthode par spectrométrie par résonance
magnétique nucléaire pulsée
ICS: 67.200.20
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2025
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
Phone: +41 22 749 01 11
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/DIS 10565:2025(en)
ii
ISO/DIS 10565:2025(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Samples . 2
5.1 General .2
5.2 Calibration samples .2
5.2.1 Samples for moisture-content calibration .2
5.2.2 Samples for oil-content calibration .2
6 Apparatus . 2
7 Sampling . 3
8 Preparation of test sample . 3
9 Calibration procedure . 3
9.1 General .3
9.2 Calibration procedure for the determination of water content .4
9.2.1 Procedure A: Three calibration samples with known water contents .4
9.2.2 Procedure B: One calibration sample with known water content .4
9.3 Calibration procedure for the determination of oil content .5
9.3.1 Procedure A: Three calibration samples with known oil contents .5
9.3.2 Procedure B: One calibration sample with known oil content.6
10 Procedure . 7
10.1 Requirements for the test room and conditioning of the test sample.7
10.2 Test portion .7
10.3 Determination .7
10.3.1 General case .7
10.3.2 Rapeseeds, linseeds and sunflower seeds having a water content greater than
10 % (m/m) and soya beans having a water content greater than 14 % (m/m) .8
11 Expression of the results . 8
12 Precision . 9
12.1 Interlaboratory tests .9
12.2 Repeatability .9
12.3 Reproducibility .9
13 Test report . 9
Annex A (informative) Results of interlaboratory tests . 10
Annex B (informative) Results of test to compare different equipment .12
Bibliography . 14
iii
ISO/DIS 10565:2025(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent
rights identified during the development of the document will be in the Introduction and/or on the ISO list of
patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical ISO/TC 34, Food products, Subcommittee SC 2, Oleaginous seeds and
fruits and oilseed meals.
This third edition cancels and replaces the second edition (ISO 10565:1998), which has been technically
revised.
The main changes compared to the previous edition are as follows:
— Clarification of the scope;
— Addition of additional information in the protocol.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
DRAFT International Standard ISO/DIS 10565:2025(en)
Oilseeds — Simultaneous determination of oil and water
contents — Method using pulsed nuclear magnetic resonance
spectrometry
1 Scope
This International Standard specifies a rapid method for the determination of the oil and water contents of
commercial oilseeds using pulsed Nuclear Magnetic Resonance (NMR).
It is applicable to rapeseeds, soya beans, linseeds and sunflower seeds with a water content less than 10 %. For
seeds with higher water contents, drying is necessary before the oil content can be determined by pulsed NMR.
NOTE 1 This method has been tested with rapeseeds, soya beans, linseeds and sunflower seeds. This does not,
however, preclude its applicability to other commercial seeds whose oil is liquid at the temperature of measurement.
NOTE 2 The reproducibility values are generally higher than those obtained by the drying method (ISO 665).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 659, Oilseeds — Determination of oil content (Reference method)
ISO 664, Oilseeds — Reduction of laboratory sample to test sample
ISO 665, Oilseeds — Determination of moisture and volatile matter content
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
4 Principle
Insertion of the test sample into the magnetic field of a pulsed NMR spectrometer.
Application of an alternating electromagnetic field in the form of an intense 90° Radiofrequency (RF) pulse
which excites all the hydrogen nuclei. Recording of the free induction decay (FID) following the 90° pulse.
The maximum amplitude of this signal is proportional to the total number of protons from the water and oil
phases of the sample.
Application of the second RF pulse, a so-called 180° pulse, to produce a spin-echo signal when only the signal
from the oil phase contributes to the FID.
NOTE 1 The maximum amplitude of this echo signal is proportional to the oil content. It varies with the sample
temperature following a complex law. An increase in temperature decreases the measured value of the echo.
ISO/DIS 10565:2025(en)
Calculation of the difference between the two amplitudes, which is proportional to the water content.
Automatic conversion of the measured signals, after suitable calibration of the apparatus, into percentages
of oil or water.
NOTE 2 Simultaneous indications of the oil and water contents can be given by some spectrometers equipped with
a minicomputer and a specific program.
5 Samples
5.1 General
Transfer the calibration and test samples to the test room at least 60 min before the determination to allow
them to reach equilibrium temperature.
For rapeseeds, linseeds and sunflower seeds having a water content greater than 10 % (m/m) and soya
beans having a water content greater than 14 % (m/m) refers to 10.3.2. In this case, the pulsed NMR and
the spin-echo method do not give a correct value because the excess water has a different behaviour and
contributes, together with the oil, to the spin-echo signal. This excess water shall therefore be removed by
drying to below 10 % for all seeds.
5.2 Calibration samples
Calibration samples shall be homogeneous and free from impurities. A definition of impurities is given in
[1]
ISO 658 .
5.2.1 Samples for moisture-content calibration
The water content of seeds can vary depending on storage conditions. Water content shall therefore be
determined in accordance with ISO 665 just prior to calibration.
5.2.2 Samples for oil-content calibration
Samples of oilseeds shall be of the same species as the test samples and of similar fatty acid compositions
(for instance for the analysis of rapeseeds which are rich in erucic acid, or sunflower seeds which are rich in
oleic acid). Oil content shall be determined using the reference method specified in ISO 659.
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
6.1 Pulsed low-resolution NMR spectrometer, suitable for measurement of the oil content and water
content of oilseeds, and meeting the precision requirements of 12.2 and 12.3.
The instrument's parameters shall be in accordance with the instructions/specifications from the
manufacturer.
For all calibration and measurement operations, follow the user's manual
The temperature of the test room shall be maintained in accordance with the instructions of the
manufacturer. Ensure that all operations during calibration and measurement are carried out under the
same conditions and, in particular, at the same temperature (±2 °C). Therefore, it is recommended that the
test room be temperature controlled.
NOTE Usual range for the test room temperature is between 17 °C and 28 °C.
CAUTION — Remove metallic objects from the proximity of the NMR spectrometer.
ISO/DIS 10565:2025(en)
6.2 Sample tubes, made of glass, suitable for use with the NMR spectrometer.
NOTE 1 Follow the manufacturers' instructions for suitable sample tube filling heights and the ranges of mass
variation recommended for different seeds.
NOTE 2 For a filling height of 30 mm with tubes of diameter 40 mm (which is equivalent to a volume of 40 mL), the
following ranges of masses are recommended:
whole rapeseeds and linseeds 22 g to 25 g
whole sunflower seeds 14 g to 17 g
whole soya beans 21 g to 24 g
For medium seeds (sunflower, soya and safflower), a volume of 40 mL minimum shall be used, in order to
guarantee the representativeness of the sample to be analysed.
6.3 Analytical balance, electronic, capable of weighing to an accuracy of ±0,01 g.
This equipment may be linked to the NMR spectrometer so that the sample mass is recorded directly by the
NMR or linked to a minicomputer (see NOTE 2 in clause 4).
6.4 Drying oven, capable of being maintained at 103 °C ± 2 °C.
6.5 Dishes, made of glass or metal, of diameter 7 cm to 10 cm, and provided with lids.
6.6 Desiccator, containing an efficient desiccant.
7 Sampling
Sampling is not part of the method specified in this International Standard. A recommended sampling
[2]
method is given in ISO 21294 .
It is important that the laboratory receive a sample which is truly representative and has not been damaged
or changed during transport or storage.
8 Preparation of test sample
Prepare the test sample in accordance with ISO 664.
Remove from the prepared test sample all metallic objects (e.g. staples, needles, etc.). Whole seeds shall be
homogeneous and, as far as possible, free from impurities.
9 Calibration procedure
9.1 General
Use the set-up parameters of the NMR spectrometer (6.1) recommended by the manufacturer and optimize
th
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.
Loading comments...