EN 28265:1994
(Main)Water quality - Design and use of quantitative samplers for benthic macro-invertebrates on stony substrata in shallow freshwaters (ISO 8265:1988)
Water quality - Design and use of quantitative samplers for benthic macro-invertebrates on stony substrata in shallow freshwaters (ISO 8265:1988)
Gives guidance on the equipment and procedures for the sampling by quadrat samplers in waters of depth less than 500 mm, although methods are described to permit sampling, under certain conditions, in a water depth of up to 1 m. The procedures are applicable to all accessible aquatic habitats in rivers, streams and estuaries where current velocities in excess of 0,1 m/s would be expected.
Wasserbeschaffenheit - Aufbau und Verwendung von quantitativen Sammelgeräten für benthische Makroinvertebraten von steinigem Untergrund seichter Süßwasser (ISO 8265:1988)
Diese Internationale Norm gibt Hinweise zur Ausrüstunf und zu Verfahren für quantitative Probenahme benthischer Makro-Invertebraten durch quadratische Probenahmegeräte in Gewässern, die weniger als 500mm tief sind. Es werden aber auch Verfahren beschrieben, die unter bestimmten Bedingungen die Probenahme bei einer Wassertiefe bis zu 1m erlauben.
Qualité de l'eau - Conception et utilisation des échantillonneurs de macro-invertébrés benthiques sur substrat rocailleux dans les eaux douces peu profondes (ISO 8265:1988)
La présente Norme internationale donne des recommandations sur les équipements et modes opératoires pour le prélèvement quantitatif de macro-invertébrés par des bacs échantillonneurs dans une profondeur d'eau inférieure à 500 mm, bien que les méthodes décrites permettent un prélèvement, dans certaines conditions, dans une profondeur d'eau allant jusqu'à 1 m. Les modes opératoires sont applicables aux prélèvements de tous les habitats aquatiques accessibles dans les rivières, les courants et les estuaires où les vitesses devraient dépasser 0,1 m/s, mais on peut utiliser les échantillonneurs, après modification, dans des endroits de faible vitesse de courant, tels que mares et lacs (voir l'annexe A). Le prélèvement peut être difficile ou impossible lorsque le substrat se compose de grosses pierres et de gros galets ou de plans de macrophytes denses (plantes à racines). Les résultats du prélèvement fournissent des données quantitatives sur la présence, la diversité et l'abondance relative de taxons.
Kakovost vode - Načrtovanje in uporaba kvantitativnih vzorčevalnikov za velike nevretenčarje usedlin na kamnitem dnu v plitvih celinskih vodah (ISO 8265:1988)
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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wasserbeschaffenheit - Aufbau und Verwendung von quantitativen Sammelgeräten für benthische Makroinvertebraten von steinigem Untergrund seichter Süßwasser (ISO 8265:1988)Qualité de l'eau - Conception et utilisation des échantillonneurs de macro-invertébrés benthiques sur substrat rocailleux dans les eaux douces peu profondes (ISO 8265:1988)Water quality - Design and use of quantitative samplers for benthic macro-invertebrates on stony substrata in shallow freshwaters (ISO 8265:1988)13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 28265:1994SIST EN 28265:1998en01-januar-1998SIST EN 28265:1998SLOVENSKI
STANDARDSIST ISO 8265:19971DGRPHãþD
INTERNATIONAL STANDARD INTERNATIONAL ORGANIZATION FOR STANDARDIZATION ORGANISATION INTERNATIONALE DE NORMALISATION MEXAYHAPOJJHAFI OPTAHM3A~MFI fl0 CTAH~APTM3A~MM ISO 8265 First edition 1988-12-15 Water quality - Design and use of quantitative Samplers for benthic macro-invertebrates on stony substrata in shallow freshwaters Qualit6 de l’eau - Concep tion et u tilisa tion des &han tiflonneurs de macro-invertkbrhs benthiques sur Substrat rocaileux dans /es eaux douces peu profondes Reference number ISO 8265 : 1988 (E) SIST EN 28265:1998SIST EN 28265:1998
ISO 8265 : 1988 (El Foreword ISO (the International Organization for Standardization) is a worldwide federation of national Standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Esch member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the ISO Council. They are approwed in accordance with ISO procedures requiring at least 75 % approval by the member bodies voting. International Standard ISO 8265 was prepared by Technical Committee ISO/TC 147, Wa ter quality . Annex A forms an integral part sf this International Standard. 0 International Organkation for Standardkation, 1988 Printed in Switzerland ii SIST EN 28265:1998SIST EN 28265:1998
ISO 8265 : 1988 (El Introduction The “Surber”l) and “cylinder” Samplers described in this International Standard are designed to remove benthic macro-invertebrates from a defined shape and area of Substrate of a bed of a water body. Hence these Samplers are sometimes called quadrat Samplers. They may be regarded as quantitative counterparts of the handnet sampling method described in ISO 7828, Water qua/-@ - Methods of biological sampling - Guidance on handnet sampling of aquatic benthic macro-invertebrates. They are usually used in water with a depth of less than 500 mm and are thus restricted to use in shallow water, particularly the “Surber”, which should preferably not be submerged. Although the Samplers tan also be used in deeper water by a diver, it must be emphasised that they were not originally designed for that purpose. Their basic design is described as part of this International Standard. Modifications are presented in annex A. 1) Named after the biologist, E. W. Surber, who originally designed this apparatus. . . . Ill SIST EN 28265:1998SIST EN 28265:1998
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INTERNATIONAL STANDARD ISO 8265 : 1988 (E) Water quality - Design and use of quantitative Samplers for benthic macro-invertebrates on stony substrata in shallow freshwaters Safety precautions - Working alone in water is not recommended in view of the risks from high current velocities, deep waters and unstable beds. 1 Scope This International Standard gives guidance on the equipment and procedures for the quantitative sampling of benthic macro- invertebrates by quadrat Samplers in waters of depth less than 500 mm, although methods are described to permit sampling, under certain conditions, in a water depth of up to 1 m. The procedures are applicable to the sampling of all accessible aquatic habitats in rivers, streams and estuaries where current velocities in excess of 0,l m/s would be expected, but the Samplers, with modifications, may be used in locations of low current velocity, such as Ponds and lakes (see annex AL Sampling may be difficult or impossible where the Substrate in- cludes large stones and boulders or dense Stands of macro- phytes (rooted plants). The results of using the Samplers pro- vide quantitative data on the presence, diversity and relative abundante of taxa. 2 Normative reference The following Standard contains provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publication, the edition indicated was valid. All Standards are subject to revision, and Parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent edition of the Standard listed below. Members of IEC and ISO maintain registers of currently valid International Standards. ISO 5667-3 : 1985, Water quality - Sampling - Part 3: Guidance on the preservation and handling of samples. 4 Apparatus 4.1 Surber Sampler The Sampler [see figure 1 a)] consists of two frames hinged together, one supporting the net and the other defining the sampling area. The whole Sampler weighs about 2 kg, folds flat and is easy to carry. Esch frame, and therefore the sampling area, is usually a Square of dimensions 300 mm x 300 mm, to give a sampling area and a net mouth of 0,09 m2 [see figure 1 b)]. Two braces leck the two frames into the working Position at right angles to each other, and two triangular wings of netting or canvas reduce the loss of invertebrates around the sides of the net. NOTE - The sutface of the sampling area is generally 0,09 m* but the frame dimensions tan be modified in Order to obtain a surface area that permits the Sampler to fulfil the objectives of the sampling. The net should be about 700 mm long, with a collar of heavier material (e.g. canvas or sail-cloth) for a short section around the mouth [sec figure 1 all. This collar increases the durability of the net, and may be extended under the net to protect it from abrasion. The original net was tapered slightly to a dome- shape at the rear end, but pocket-shaped and cone-shaped nets are now frequently used. In figure 1 d) the net is shown with a collar of sail-cloth for a short section around the mouth and extended under the net. Another and stronger construction of a Surber Sampler is shown in figure 2, in which the two frames are replaced by a box with an open bottom and top, solid sides, a net on the downstream side and a Screen on the upstream side. The box minimizes the loss of invertebrates and in addition gives the Sampler additional strength and stability. The Sampler is also easier to operate than the original in high current velocities. The choice of mesh size depends on the objectives of the Survey. Table 1 gives details of appropriate sizes. 3 Principle 4.2 Cylinder Sampler Sampling of benthic macro-invertebrates in shallow running water by collection using a quadrat Sampler, which isolates a Portion of the bed of the water body. Disturbance of the sub- strate leads to release of macro-invertebrates, from attachment or burial, and they are carried into the net by the current. The Sampler consists essentially of an open-ended cylinder, constructed of about 0,5 mm thick stainless steel, having the lower edge serrated with teeth, each 10 mm deep. The upper edge may be covered by a plastics edging Strip to protect SIST EN 28265:1998SIST EN 28265:1998
ISO 6265 : 1988 (El the Operator. Handles on the sides facilitate pushing the Sampler into the stream bed. To allow water to enter the Sampler, an oval aperture should be tut in one side of the cylinder near the lower edge. To reduce the entry of drift organisms, this aperture is fitted with a stainless steel Screen of coarse mesh of aperture about 1 mm diameter. Opposite this opening, a second hole is tut and is fitted with a short exit port to which a detachable collecting net is attached (see figure 3). Selection of the appropriate mesh size depends upon the nature of the investigation but guidance is given in table 1. The net should be 500 mm deep and is constructed of nylon with a 50 mm deep canvas collar which holds a draw cord for attach- ment to the exit Port. A shallow flange on the distal edge of the exit port ensures secure attachment. Two sizes of cylinder are in general use, with horizontal Cross-sectional areas of 0,05 m2 and of 0,l m2. The depth of both cylinders is usually about 450 mm (figure 4). The mass of the 0,05 m2sampler is 3 kg and that of the 0,l m2 Sampler is approximately 4 kg. Figure 4 gives a recommended Pattern for marking out on a flat metal sheet, together with some details of construction, for a Sampler of area 0,05 m? 5 Sampling procedure and samples 5.1 Choice of Sampler The choice between the Surber-type Sampler and the cylinder- type Sampler depends on personal preference based on operating experience and the prevailing conditions. In slow- flowing water
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