EN 13783:2001

Foodstuffs - Detection of irradiated food using Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) - Screening method

This European Standard specifies a microbiological screening method for the detection of irradiation treatment of herbs and spices, using the combined direct epifluorescent filter technique (DEFT) and aerobic plate count (APC). The DEFT/APC technique is not radiation specific, therefore, it is recommended to confirm positive results using a standardised method (e.g. EN 1788, prEN 13751) to specifically prove an irradiation treatment of the suspected food.
The method has been successfully tested in interlaboratory tests with herbs and spices [1] to [5].

Lebensmittel - Nachweis der Bestrahlung von Lebensmitteln mit Epifluoreszenz-Filtertechnik/aerober mesophiler Keimzahl (DEFT/APC) - Screeningverfahren

Diese Europäische Norm legt ein mikrobiologisches Screeningverfahren zum Nachweis einer Bestrahlung von Kräutern und Gewürzen fest, wobei die Kombination der Epifluoreszenz-Mikroskopie von Filtern (direct epifluorescent filter technique, DEFT) und der aeroben mesophilen Keimzahl (aerobic plate count, APC) angewandt wird. Die DEFT/APC-Technik ist nicht spezifisch für eine erfolgte Bestrahlung, deshalb wird empfohlen, positive Befunde durch Anwendung eines genormten Verfahrens (z. B. EN 1788, prEN 13751) zum spezifischen Nachweis einer Bestrahlung des jeweiligen Lebensmittels zu bestätigen.
Dieses Verfahren wurde in Ringversuchen mit Kräutern und Gewürzen erfolgreich getestet [1] bis [5].

Produits alimentaires - Détection d'aliments ionisés en utilisant la technique d'épifluorescence après filtration et dénombrement de la flore aérobie sur milieu gélosé (DEFT/APC) - Méthode par criblage

Le présent projet de Norme européenne spécifie une méthode de criblage microbiologique permettant de détecter les herbes et les épices ayant subi un traitement ionisant, en utilisant à la fois la technique d'épifluorescence directe après filtration (DEFT) et le dénombrement de la flore aérobie sur milieu gélosé (APC). La technique DEFT/APC n'étant pas spécifique des traitements ionisants, il est donc recommandé de confirmer les résultats positifs par une méthode normalisée (par exemple EN 1788 et prEN 13751) permettant de prouver spécifiquement que les aliments suspectés ont subi un traitement ionisant.
La méthode s'est révélée satisfaisante lors des essais interlaboratoires portant sur les herbes et des épices [1] à [5].

Živila - Detekcija obsevane hrane z uporabo direktne tehnike epifluoroscentnega filtra/aerobično štetje na plošči (DEFT/APC) - Informativna metoda

General Information

• Status: Published
• Publication Date: 20-Nov-2001
• Withdrawal Date: 30-May-2002
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 8 - Irradiated foodstuffs
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 05-Jun-2023
• Ref Project: SIST EN 13783:2002 - Foodstuffs - Detection of irradiated food using Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) - Screening method

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.OXRURVFHQWQHJDDLebensmittel - Nachweis der Bestrahlung von Lebensmitteln mit Epifluoreszenz-Filtertechnik/aerober mesophiler Keimzahl (DEFT/APC) - ScreeningverfahrenProduits alimentaires - Détection d'aliments ionisés en utilisant la technique d'épifluorescence apres filtration et dénombrement de la flore aérobie sur milieu gélosé (DEFT/APC) - Méthode par criblageFoodstuffs - Detection of irradiated food using Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) - Screening method67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 13783:2001SIST EN 13783:2002en01-junij-2002SIST EN 13783:2002SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 13783November 2001ICS 07.100.30; 67.050English versionFoodstuffs - Detection of irradiated food using DirectEpifluorescent Filter Technique/Aerobic Plate Count(DEFT/APC) - Screening methodProduits alimentaires - Détection d'aliments ionisés enutilisant la technique d'épifluorescence après filtration etdénombrement de la flore aérobie sur milieu gélosé(DEFT/APC) - Méthode par criblageLebensmittel - Nachweis der Bestrahlung vonLebensmitteln mit Epifluoreszenz-Filtertechnik/aerobermesophiler Keimzahl (DEFT/APC) - ScreeningverfahrenThis European Standard was approved by CEN on 29 September 2001.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2001 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 13783:2001 ESIST EN 13783:2002

Further information on the applicability.12Annex B (informative)
Practical example for calculation of the microscope factor.13Annex C (informative)
Flow diagram of the procedure.14Bibliography.15ForewordThis European Standard has been prepared by Technical Committee CEN /TC 275 "Food Analysis - HorizontalMethods", the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, either by publication of an identical text orby endorsement, at the latest by May 2002, and conflicting national standards shall be withdrawn at the latest byMay 2002.This European Standard was elaborated on the basis of a protocol developed following a concerted actionsupported by the Commission of European Union (XII C.) (BCR), and a screening investigation carried out by theDanish National Food Agency and the local Environment and food Agency, MLK FYN, DK Odense. Experts andlaboratories from E.U. and EFTA countries, contributed jointly to the development of the concerted action protocol.WARNING: The use of this standard may involve hazardous materials, operations and equipment. This standarddoes not purport to address all the safety problems associated with its use. It is the responsibility of the user of thisstandard to establish appropriate safety and health practices and determine the applicability of regulatorylimitations prior to use.The annexes A, B and C are informative.This standard includes a Bibliography.According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland,France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden,Switzerland and the United Kingdom.SIST EN 13783:2002

CompositionCitric acid solution (4.3.1)100 mlSodium hydroxide solution (4.3.2)54 ml4.3.3.2 PreparationMix the citric acid solution and the sodium hydroxide solution. Adjust pH to 3,0 ± 0,2 with citric acid solution orsodium hydroxide solution. Sterilize the buffer through a membrane filter of pore size 0,2 µm (5.3) before use. Thesolution may be stored in a glass bottle at 4 °C to 6 °C for not more than three weeks.SIST EN 13783:2002

PreparationDissolve the acridine orange in the buffer solution (4.4.1). Sterilize the acridine orange solution through a 0,2 µmpore size membrane filter (5.3) before use. The solution may be stored in a brown glass bottle at 4 °C to 6 °C fornot more than one week.NOTE 1 Concentrated acridine orange solution is commercially available and is recommended for safety reasons.NOTE 2 As acridine orange is regarded as a mutagenic substance, disposable gloves and face masks should be used whenweighing the stain.4.5 2-Propanol4.6 Triton® X-1001), 1 % cleaning solution4.6.1 CompositionTriton® X – 10010 mlDistilled or demineralized water1000 ml4.6.2 PreparationMix Triton X-100 with warm (80°C) water. Sterilize the solution through a 0,2 µm pore size membrane filter (5.3).The solution may be stored in a glass bottle at 4 °C to 6 °C for not more than three weeks.
1) Triton® X-100 is an example of a suitable product available commercially. This information is only given for the convenienceof users of this International Standard and does not constitute an endorsement by CEN of this product.SIST EN 13783:2002
...