EN ISO 10272-2:2017

Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique (ISO 10272-2:2017)

ISO 10272-2:2017 specifies a horizontal method for the enumeration of Campylobacter spp. It is applicable to
-      products intended for human consumption,
-      products intended for animal feeding,
-      environmental samples in the area of food and feed production, handling, and
-      samples from the primary production stage such as animal faeces, dust, and swabs.

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zählung von Campylobacter - Teil 2: Koloniezählverfahren (ISO 10272-2:2017)

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le dénombrement de Campylobacter spp. - Partie 2 : Technique par comptage des colonies (ISO 10272-2:2017)

L'ISO 10272-2:2017 spécifie une méthode horizontale de dénombrement de Campylobacter spp. Il s'applique:
-      aux produits destinés à la consommation humaine;
-      aux produits destinés à l'alimentation des animaux;
-      aux échantillons environnementaux prélevés dans les secteurs de la production et de la manutention des aliments; et
-      aux échantillons au stade de la production primaire tels que les matières fécales des animaux, la poussière et les prélèvements de surface.

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Campylobacter spp. - 2. del: Tehnika štetja kolonij (ISO 10272-2:2017)

Ta del standarda opisuje metodo za ugotavljanje števila Campylobacter spp. s tehniko štetja kolonij (referenčni dokument je standard ISO/TS 10272 -2).

General Information

• Status: Published
• Publication Date: 04-Jul-2017
• Withdrawal Date: 30-Jan-2018
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 3 - Campylobacter
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 05-Jul-2017
• Due Date: 28-Feb-2014
• Completion Date: 05-Jul-2017
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/381 - Mandate for standardisation addressed to CEN in the field of Methods of analysis of foodstuffs concerning Food Hygiene
• Ref Project: SIST EN ISO 10272-2:2017 - Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique (ISO 10272-2:2017)

Relations

• Amended By: EN ISO 10272-2:2017/A1:2023 - Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique - Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and changes in the performance testing of culture media (ISO 10272-2:2017/Amd 1:2023)
• Effective Date: 30-Jan-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2017
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti in števila Campylobacter spp. - 2. del: Tehnika štetja kolonij (ISO 10272
-2:2017)
Microbiology of the food chain - Horizontal method for detection and enumeration of
Campylobacter spp. - Part 2: Colony-count technique (ISO 10272-2:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur
Zählung von Campylobacter - Teil 2: Koloniezählverfahren (ISO 10272-2:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le
dénombrement de Campylobacter spp. - Partie 2 : Technique par comptage des colonies
(ISO 10272-2:2017)
Ta slovenski standard je istoveten z: EN ISO 10272-2:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10272-2
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2017
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for
detection and enumeration of Campylobacter spp. - Part 2:
Colony-count technique (ISO 10272-2:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche et le dénombrement de Verfahren zum Nachweis und zur Zählung von
Campylobacter spp. - Partie 2 : Technique par Campylobacter - Teil 2: Koloniezählverfahren (ISO
comptage des colonies (ISO 10272-2:2017) 10272-2:2017)
This European Standard was approved by CEN on 1 May 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10272-2:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 10272-2:2017) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2018 and conflicting national standards shall
be withdrawn at the latest by January 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 10272-2:2017 has been approved by CEN as EN ISO 10272-2:2017 without any
modification.
INTERNATIONAL ISO
STANDARD 10272-2
First edition
2017-06
Microbiology of the food chain —
Horizontal method for detection and
enumeration of Campylobacter spp. —
Part 2:
Colony-count technique
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Campylobacter spp. —
Partie 2: Technique par comptage des colonies
Reference number
ISO 10272-2:2017(E)
©
ISO 2017
ISO 10272-2:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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ii © ISO 2017 – All rights reserved

ISO 10272-2:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Preparation of dilutions . 2
4.3 Enumeration . 2
4.4 Confirmation . 2
5 Culture media and reagents . 2
6 Equipment and consumables . 3
7 Sampling . 3
8 Preparation of test sample . 3
9 Procedure. 4
9.1 Test portion, initial suspension and dilutions . 4
9.2 Inoculation and incubation . 4
9.3 Enumeration of characteristic colonies . 4
9.4 Confirmation of Campylobacter . 4
9.4.1 General. 4
9.4.2 Selection of colonies for confirmation . 5
9.4.3 Examination of morphology and motility . 5
9.4.4 Study of aerobic growth at 25 °C . 5
9.4.5 Detection of oxidase activity . 5
9.4.6 Interpretation . 5
9.5 Identification of Campylobacter species (optional) . 6
9.5.1 General. 6
9.5.2 Detection of catalase activity. 6
9.5.3 Detection of hippurate hydrolysis . 6
9.5.4 Detection of indoxyl acetate hydrolysis . 6
9.5.5 Interpretation . 7
10 Expression of results . 7
11 Performance characteristics of the method . 7
11.1 Interlaboratory study . 7
11.2 Repeatability limit . 7
11.3 Reproducibility limit . 8
12 Test report . 9
Annex A (normative) Diagram of procedure .10
Annex B (normative) Culture media and reagents .11
Annex C (informative) Method validation studies and performance characteristics .16
Bibliography .19
ISO 10272-2:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a
...