EN ISO 10712:1995

Water quality - Pseudomonas putida growth inhibition test (pseudomonas cell multiplication inhibition test)(ISO 10712:1995)

Describes a test method for determining the inhibitory effect of surface, ground and waste water on Pseudomonas putida growth. Not suitable for highly coloured samples or samples containing undissolved or volatile substances which react with the nutrient solution or undergo changes such as biochemical degradation. Also suitable for testing substances soluble in water.

Wasserbeschaffenheit - Pseudomonas putida Wachstumshemmtest (Pseudomonas-Zellvermehrungshemmtest) (ISO 10712:1995)

Diese Internationale Norm legt ein Verfahren zur Bestimmung der Hemmwirkung von Wasser, Abwasser und wasserlöslichen Stoffen gegenüber Pseudomonas putida fest. Das Verfahren ist nicht geeignet für stark gefärbtes Testgut oder Proben, die ungelöste oder flüchtige Stoffe oder solche, die mit der Nährlösung reagieren, enthalten oder die sich während des Tests verändern (z.B. durch Ausfällung, biochemischen oder photochemischen Abbau) und verfälschte Ergebnisse liefern können und/oder die Reproduzierbarkeit beeinträchtigen.

Qualité de l'eau - Essai d'inhibition de la croissance de Pseudomonas putida (essai d'inhibition de la multiplication des cellules de Pseudomonas) (ISO 10712:1995)

La présente Norme internationale prescrit une méthode d'essai pour la détermination de l'effet inhibiteur de l'eau de surface, de l'eau souterraine et des eaux usées vis-à-vis de Pseudomonas putida. La méthode n'est pas applicable aux échantillons de forte coloration, ou contenant des matières non dissoutes ou volatiles ou des substances réagissant avec le milieu nutritif ou qui subissent des changements pendant l'essai (par exemple par précipitation, ou dégradation biochimique ou photochimique), et peuvent donner des résultats faux, et/ou affecter la reproductibilité. La méthode permet également d'expérimenter les substances solubles dans l'eau (voir annexe A).

Kakovost vode - Preskus z zaviranjem rasti bakterije Pseudomonas putida (Preskušanje z zaviranjem razmnoževanja bakterije Pseudomonas) (ISO 10712:1995)

General Information

• Status: Published
• Publication Date: 14-Dec-1995
• Withdrawal Date: 29-Jun-1996
• ICS: 13.060 - Water quality
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 21-May-2008
• Completion Date: 21-May-2008
• Ref Project: SIST EN ISO 10712:1997 - Water quality - Pseudomonas putida growth inhibition test (pseudomonas cell multiplication inhibition test)(ISO 10712:1995)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 10712:1997
01-avgust-1997
Kakovost vode - Preskus z zaviranjem rasti bakterije Pseudomonas putida
(Preskušanje z zaviranjem razmnoževanja bakterije Pseudomonas) (ISO
10712:1995)
Water quality - Pseudomonas putida growth inhibition test (pseudomonas cell
multiplication inhibition test)(ISO 10712:1995)
Wasserbeschaffenheit - Pseudomonas putida Wachstumshemmtest (Pseudomonas-
Zellvermehrungshemmtest) (ISO 10712:1995)
Qualité de l'eau - Essai d'inhibition de la croissance de Pseudomonas putida (essai
d'inhibition de la multiplication des cellules de Pseudomonas) (ISO 10712:1995)
Ta slovenski standard je istoveten z: EN ISO 10712:1995
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 10712:1997 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 10712:1997

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SIST EN ISO 10712:1997

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SIST EN ISO 10712:1997

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SIST EN ISO 10712:1997

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SIST EN ISO 10712:1997

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SIST EN ISO 10712:1997
INTERNATIONAL
IS0
STANDARD
10712
First edition
1995-l 2-15
Water quality - Pseudomonas putida
growth inhibition test (Pseudomonas cell
multiplication inhibition test)
Qualit6 de I’eau - Essai d’inhibition de la croissance de Pseudomonas
putida (essai d’inhibition de la multiplication des cellules de
Pseudomonas)
Reference number
IS0 10712:1995(E)

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SIST EN ISO 10712:1997
IS0 10712:1995(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide
federation of national standards bodies (IS0 member bodies). The work
of preparing International Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. IS0
collaborates closely with the International Electrotechnical Commission
(I EC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard IS0 10712 was prepared by Technical Committee
ISODC 147, INater quality, Subcommittee SC 5, Biological methods.
Annexes A and B of this International Standard are for information only.
0 IS0 1995
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-1211 Geneve 20 l Switzerland
Printed in Switzerland
ii

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SIST EN ISO 10712:1997
0 IS0
IS0 10712:1995(E)
Introduction
The bacterium Pseudomonas putida is used as an organism representative
of heterotrophic microorganisms in fresh water.

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SIST EN ISO 10712:1997
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SIST EN ISO 10712:1997
INTERNATIONAL STANDARD 0 ISO IS0 10712:1995(E)
Water quality - Pseudomonas putida growth
inhibition test (Pseudomonas cell multiplication
inhibition test)
3.1 multiplication; growth: Increase in the number
1 Scope
of cells during the test period.
This International Standard specifies a test method for
determining the inhibitory effect of surface, ground 3.2 concentration-effect relationship: Depen-
and waste water on Pseudomonas putida. dence of cell multiplication inhibition on the concen-
tration of the test sample.
This method is not suitable for highly coloured test
samples, or samples containing undissolved or volatile
NOTE 1 The relationship is graphically represented by
materials or substances which react with the nutrient plotting the inhibition values along the ordinate against the
sample concentrations along the abscissa.
solution, or which undergo changes during the test
(for example by precipitation, or biochemical or
3.3 effective concentration (EC): Concentration of
photochemical degradation) and may give false results
the test sample giving a calculated or interpolated
and/or impair the reproducibility.
inhibition of cell multiplication of Pseudomonas
...