EN ISO 24032:2021
(Main)Soil quality - In situ caging of snails to assess bioaccumulation of contaminants (ISO 24032:2021)
Soil quality - In situ caging of snails to assess bioaccumulation of contaminants (ISO 24032:2021)
This document describes a method to assess the bioaccumulation of chemicals in snails, i.e. concentrations of metal(loid)s (ME) or organic compounds [e.g. polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs)] accumulated in their tissues.
This document presents how to prepare snails for caging in situ for 28 days, the in situ test design and then how to collect and prepare the snails until conservation and further analysis. If a kinetic study of accumulation is necessary, sampling of snails at different time-points during exposure is possible as well [13],[19],[22].
This document excludes analytical methods. Preparation (extraction and mineralization) of the samples and quantification of chemicals are not in the scope of the present document.
The method is applicable for soils under different uses (agricultural, industrial, residential, forests, before and after remediation, on potentially contaminated sites, etc.) and waste materials [8],[10], preferably with vegetation and/or humus cover.
The method is applicable subject to certain limits of temperature (frost-free period, i.e. mainly from April to October in temperate region).
Optionally (see Annex I), the method can be used in the laboratory to evaluate the accumulation of contaminants [and optionally, the sum of excess of transfer (SET) index for ME, PAH, PCB] of snails exposed only to soil.
Bodenbeschaffenheit - In-situ-Käfighaltung von Schnecken zur Beurteilung der Bioakkumulation von Kontaminanten (ISO 24032:2021)
Dieses Dokument beschreibt ein Verfahren zur Bewertung der Bioakkumulation von Chemikalien in Schnecken, d. h. Konzentrationen von Metall(oid)en (ME) oder organischen Verbindungen, z. B. polyzyklische aromatische Kohlenwasserstoffe (PAK) und polychlorierte Biphenyle (PCB), die sich in ihren Geweben angereichert haben.
In diesem Dokument wird dargelegt, wie Schnecken für die 28 tägige In situ-Käfighaltung vorbereitet werden, wie das In situ-Prüfdesign aussieht und wie die Schnecken bis zur Konservierung und weiteren Analyse gesammelt und vorbereitet werden. Falls eine kinetische Untersuchung der Akkumulation notwendig ist, ist auch eine Probenahme der Schnecken zu verschiedenen Zeitpunkten während der Exposition möglich [13] [19] [22].
Dieses Dokument schließt analytische Verfahren aus. Die Vorbereitung (Extraktion und Mineralisierung) der Proben und Quantifizierung von Chemikalien fallen nicht in den Anwendungsbereich des vorliegenden Dokuments.
Das Verfahren ist auf Böden mit unterschiedlicher Nutzung (landwirtschaftliche, industrielle, Wohn und Waldböden, vor und nach der Sanierung, auf potenziell kontaminierten Standorten usw.) sowie auf Abfallmaterialien [8] [10] anwendbar, vorzugsweise auf Böden mit Vegetation und/oder Humusbedeckung.
Das Verfahren ist vorbehaltlich bestimmter Temperaturgrenzen (frostfreier Zeitraum, d. h. hauptsächlich von April bis Oktober in gemäßigtem Klima) anwendbar.
Optional (siehe Anhang I) kann das Verfahren im Labor verwendet werden, um die Akkumulation von Kontaminanten (und optional die Summe des Übertragungsüberschusses (en: Sum of Excess of Transfer) (SET) index für ME, PAK, PCB) von Schnecken zu bewerten, die nur dem Boden exponiert sind.
Qualité du sol - Encagement in situ d’escargots pour la mesure de la bioaccumulation de contaminants (ISO 24032:2021)
Le présent document décrit une méthode permettant d’évaluer la bioaccumulation de substances chimiques chez les escargots, c’est-à-dire les concentrations de métaux (métalloïdes) (ME) ou de composés organiques [tels que les hydrocarbures aromatiques polycycliques (HAP) et les polychlorobiphényles (PCB)] accumulés dans leurs tissus.
Le présent document présente la méthodologie pour préparer les escargots pour un encagement in situ pendant 28 jours, la description de l’essai in situ, puis la méthodologie de collecte et de préparation des escargots jusqu’à leur conservation pour analyse ultérieure. Si une étude cinétique de l’accumulation est nécessaire, il est également possible de prélever des échantillons d’escargots à différents moments de l’exposition[13],[19],[22].
Le présent document exclut les méthodes analytiques. La préparation (extraction et minéralisation) des échantillons et la quantification des substances chimiques ne font pas partie du domaine d’application du présent document.
La méthode est applicable à des sols destinés à différentes utilisations (agricole, industrielle, résidentielle, forestière, avant et après dépollution, sur des sites potentiellement pollués, etc.) et aux déchets[8],[10], de préférence sur des sols recouverts d’une couverture végétale et/ou d’une couche d’humus.
La méthode est applicable sous réserve de certaines limites de température (période sans gel, c’est-à-dire généralement d’avril à octobre en région tempérée).
En option (voir Annexe I), la méthode peut être utilisée en laboratoire pour évaluer l’accumulation de contaminants [et, facultativement, l’indice Somme des excès de transfert (SET) pour les ME, HAP et PCB] des escargots exposés uniquement au sol.
Kakovost tal - Uporaba kletk s polži na terenu za oceno bioakumulacije onesnaževal (ISO 24032:2021)
Ta dokument opisuje metodo za ocenjevanje bioakumulacije kemikalij v polžih, tj. koncentracij kovin in metaloidov (ME) oziroma organskih spojin [npr. policikličnih aromatskih ogljikovodikov (PAH) in polikloriranih bifenilov (PCB)], nakopičenih v njihovih tkivih. V tem dokumentu je predstavljeno, kako pripraviti polže za 28-dnevno nastanitev v kletke na kraju samem, vključno z načrtom preskusa na kraju samem ter nadalje s postopkom zbiranja in priprave polžev do skladiščenja oziroma nadaljnje analize. Če je potrebna kinetična študija akumulacije, je mogoče izvesti tudi vzorčenje polžev ob različnem času med izpostavljenostjo [13], [19], [22]. Ta dokument vključuje analitične metode. Priprava (ekstrakcija in mineralizacija) vzorcev in količinska opredelitev kemikalij ne spadata na področje uporabe tega dokumenta. Metoda se uporablja za tla v okviru različnih vrst uporabe (kmetijska, industrijska ali stanovanjska uporaba, gozdovi, pred sanacijo in po njej, na potencialno onesnaženih območjih itd.) in odpadne materiale [8], [10], po možnosti z rastlinjem in/ali humusom. Metoda se uporablja ob upoštevanju določenih omejitev glede temperature (obdobje brez zmrzali, tj. od aprila do oktobra v območjih z zmernim podnebjem). Metodo je mogoče izbirno (glej dodatek I) uporabiti v laboratoriju za vrednotenje akumulacije onesnaževal [in izbirno indeksa vsote presežnega prenosa (SET) za kovine in metaloide, policiklične aromatske ogljikovodike ter poliklorirane bifenile] v polžih, izpostavljenih izključno tlom.
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2022
Kakovost tal - Uporaba kletk s polži na terenu za oceno bioakumulacije
onesnaževal (ISO 24032:2021)
Soil quality - In situ caging of snails to assess bioaccumulation of contaminants (ISO
24032:2021)
Bodenbeschaffenheit - In-situ-Käfighaltung von Schnecken zur Beurteilung der
Bioakkumulation von chemischen Stoffen (ISO 24032:2021)
Qualité du sol - Encagement in situ d’escargots pour la mesure de la bioaccumulation de
contaminants (ISO 24032:2021)
Ta slovenski standard je istoveten z: EN ISO 24032:2021
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 24032
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2021
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - In situ caging of snails to assess
bioaccumulation of contaminants (ISO 24032:2021)
Qualité du sol - Encagement in situ d'escargots pour la Bodenbeschaffenheit - In-situ-Käfighaltung von
mesure de la bioaccumulation de contaminants (ISO Schnecken zur Beurteilung der Bioakkumulation von
24032:2021) chemischen Stoffen (ISO 24032:2021)
This European Standard was approved by CEN on 5 December 2021.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
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Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
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United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 24032:2021 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 24032:2021) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 24032:2021 has been approved by CEN as EN ISO 24032:2021 without any modification.
INTERNATIONAL ISO
STANDARD 24032
First edition
2021-12
Soil quality — In situ caging of
snails to assess bioaccumulation of
contaminants
Qualité du sol — Encagement in situ d’escargots pour la mesure de la
bioaccumulation de contaminants
Reference number
ISO 24032:2021(E)
ISO 24032:2021(E)
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
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Published in Switzerland
ii
ISO 24032:2021(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Test organism and equipment . 2
5.1 Biological material . 2
5.2 Equipment . 3
6 Preparation of the organisms for the exposure . 4
7 Exposure of the test organisms . 4
7.1 General . 4
7.2 Beginning of exposure . 5
7.3 End of the exposure — Starvation . 6
7.4 Sampling and preparation after exposure . 7
8 Calculation and expression .7
8.1 General . 7
8.2 For metal(loid)s . 7
8.2.1 Threshold guide value . 7
8.2.2 Calculation of the sum of the excess of transfer of metal(loid)s: SET index . 8
8.3 For other chemicals . 9
9 Validity of the experiment . 9
10 Test report . 9
Annex A (informative) Sources and routes of exposure of snails to contaminants in the field .10
Annex B (informative) Main steps of the bioassay in situ .11
Annex C (informative) Breeding technique for snails .15
Annex D (informative) Example of composition of snail feed .22
Annex E (informative) Usual concentrations in the viscera of sub-adult snails before caging.23
Annex F (informative) Recommended test systems for in situ exposure to assess
bioaccumulation of contaminants in snails .25
Annex G (informative) Example of mass of snails before exposure .28
Annex H (informative) Results of the international ring test .30
Annex I (informative) Ex situ exposure to assess bioaccumulation of chemicals in snails .48
Bibliography .56
iii
ISO 24032:2021(E)
Foreword
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This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological characterization, in collaboration with the European Committee for Standardization (CEN)
Technical Committee CEN/TC 444, Environmental characterization of solid matrices, in accordance with
the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
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