Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage (ISO 6887-6:2013)

ISO 6887-6:2013 specifies rules for the preparation of samples taken at all stages from the farm to the slaughterhouse and their suspension for microbiological examination when the samples require different preparation from the methods described in ISO 6887-1.
ISO 6887-6:2013 excludes the preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards.
ISO 6887-6:2013 is applicable to various samples taken from the hatchery, the farm, from the vehicle or the animals during transportation, or from animals or their carcasses in the slaughterhouse, to indicate the microbiological status of the animals in relation to zoonotic agents. ISO 6887-6:2013 does not apply to samples taken to assess the hygiene of meat.

Mikrobiologie von Lebensmitteln und Futtermitteln - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 6: Spezifische Regeln für die Vorbereitung von Proben aus der Primärproduktion (ISO 6887-6:2013)

Dieser Teil von ISO 6887 legt die Regeln für die Vorbereitung von Proben fest, die bei allen Stufen vom Bauernhof bis zum Schlachthof entnommen werden und deren Verdünnung für mikrobiologische Unter-suchungen, wenn die Proben eine Vorbereitung erfordern, die sich von den in ISO 6887 1 beschriebenen Verfahren unterscheidet. ISO 6887 1 legt die allgemeinen Regeln für die Herstellung der Erstverdünnung und der Dezimalverdünnungen für mikrobiologische Untersuchungen fest.
Dieser Teil von ISO 6887 gilt nicht für die Vorbereitung von Proben sowohl für die Zählung als auch für die Untersuchungsverfahren zum Nachweis, bei denen Einzelheiten der Vorbereitung in den betreffenden Internationalen Normen festgelegt sind.
Dieser Teil von ISO 6887 ist bei verschiedenartigen Proben anwendbar, wie Proben, die in Brütereien, auf Bauernhöfen, von Fahrzeugen oder von Tieren während des Transports und von Tieren oder deren Schlachtkörpern in Schlachthöfen entnommen werden, um den mikrobiologischen Status der Tiere in Bezug auf zoonotische Krankheitserreger aufzuzeigen. Dieser Teil von ISO 6887 gilt nicht für Proben, die zur Beurteilung der Hygiene von Fleisch entnommen werden.
Dieser Teil berücksichtigt keine Proben, die aus der aquatischen Umwelt (Meer- oder Süßwasser) aus der Primärproduktion entnommen werden. Für diese gilt ISO 6887-3.

Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 6: Règles spécifiques pour la préparation des échantillons prélevés au stade de production primaire (ISO 6887-6:2013)

L'ISO 6887-6:2013 spécifie des règles pour la préparation d'échantillons prélevés à toutes les étapes depuis l'élevage jusqu'à l'abattoir et leur suspension pour examen biologique lorsque les échantillons nécessitent une méthode de préparation différente de celles décrites dans l'ISO 6887-1.
L'ISO 6887-6:2013 n'inclut pas la préparation d'échantillons destinés à des méthodes de recherche et de dénombrement dont les détails concernant la préparation sont spécifiés dans les Normes internationales appropriées.
L'ISO 6887-6:2013 est applicable à différents échantillons prélevés dans un couvoir, un élevage, un véhicule ou sur les animaux pendant le transport, ou sur les animaux ou leur carcasse à l'abattoir, afin d'indiquer le statut microbiologique des animaux par rapport à des agents zoonotiques. Elle ne s'applique pas aux échantillons prélevés pour évaluer l'hygiène de la viande.

Mikrobiologija živil in krme - Priprava vzorcev za preskušanje ter osnovnih in decimalnih razredčin za mikrobiološko preiskavo - 6. del: Posebna pravila za pripravo vzorcev iz primarne proizvodne faze (ISO 6887-6:2013)

Ta del standarda ISO 6887 določa pravila za pripravo vzorcev, ki se jemljejo na vseh stopnjah, tj. od kmetije do klavnice, in njihove razredčine za mikrobiološko preiskavo, kadar se za vzorce zahteva drugačna priprava od metod iz standarda ISO 6887-1. Standard ISO 6887-1 določa splošna pravila za pripravo osnovnih in decimalnih razredčin za mikrobiološko preiskavo. Ta del standarda ISO 6887 ne zajema priprave vzorcev za preskusni metodi za ugotavljanje števila in odkrivanje, kadar so podrobnosti glede priprave opredeljene v ustreznih mednarodnih standardih. Ta del standarda ISO 6887 se uporablja za različne vzorce, odvzete v valilnici, na kmetiji, v vozilu ali od živali med prevozom ali od živali ali njihovih trupov v klavnici, da se ugotovi mikrobiološki status živali v zvezi s povzročitelji zoonoze. Ta del standarda ISO 6887 se ne uporablja za vzorce, ki se jemljejo za oceno higiene mesa. Ti vzorci so obravnavani v standardu EN 6887-2. Ta del standarda ISO 6887 ne obravnava vzorcev iz vodnega okolja (morskega ali sladkovodnega) v primarni proizvodni fazi. Ti vzorci so obravnavani v standardu EN 6887-3.

General Information

Status
Published
Publication Date
05-Mar-2013
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Due Date
24-Sep-2013

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SLOVENSKI STANDARD
SIST EN ISO 6887-6:2013
01-junij-2013
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Microbiology of food and animal feed - Preparation of test samples, initial suspension

and decimal dilutions for microbiological examination - Part 6: Specific rules for the

preparation of samples taken at the primary production stage (ISO 6887-6:2013)
Mikrobiologie von Lebensmitteln und Futtermitteln - Vorbereitung von
Untersuchungsproben und Herstellung von Erstverdünnungen und von

Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 6: Spezifische Regeln

für die Vorbereitung von Proben aus der Primärproduktion (ISO 6887-6:2013)

Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des

dilutions décimales en vue de l'examen microbiologique - Partie 6: Règles spécifiques

pour la préparation des échantillons prélevés au stade de production primaire (ISO 6887-

6:2013)
Ta slovenski standard je istoveten z: EN ISO 6887-6:2013
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 6887-6:2013 en

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 6887-6:2013
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SIST EN ISO 6887-6:2013
EUROPEAN STANDARD
EN ISO 6887-6
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2013
ICS 07.100.30
English Version
Microbiology of food and animal feed - Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination - Part 6: Specific rules for the
preparation of samples taken at the primary production stage
(ISO 6887-6:2013)

Microbiologie des aliments - Préparation des échantillons, Mikrobiologie von Lebensmitteln und Futtermitteln -

de la suspension mère et des dilutions décimales en vue Vorbereitung von Untersuchungsproben und Herstellung

de l'examen microbiologique - Partie 6: Règles spécifiques von Erstverdünnungen und von Dezimalverdünnungen für

pour la préparation des échantillons prélevés au stade de mikrobiologische Untersuchungen - Teil 6: Spezifische

production primaire (ISO 6887-6:2013) Regeln für die Vorbereitung von Proben aus der

Primärproduktion (ISO 6887-6:2013)
This European Standard was approved by CEN on 16 February 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same

status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United

Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6887-6:2013: E

worldwide for CEN national Members.
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SIST EN ISO 6887-6:2013
EN ISO 6887-6:2013 (E)
Contents Page

Foreword ....................................................................................................................................................... 3

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SIST EN ISO 6887-6:2013
EN ISO 6887-6:2013 (E)
Foreword

This document (EN ISO 6887-6:2013) has been prepared by Technical Committee CEN/TC 275 “Food

analysis - Horizontal methods”, the secretariat of which is held by DIN, in collaboration with Technical

Committee ISO/TC 34 "Food products".

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by September 2013, and conflicting national standards shall be

withdrawn at the latest by September 2013.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech

Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,

Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,

Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

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SIST EN ISO 6887-6:2013
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SIST EN ISO 6887-6:2013
INTERNATIONAL ISO
STANDARD 6887-6
First edition
2013-03-01
Microbiology of food and animal
feed — Preparation of test samples,
initial suspension and decimal
dilutions for microbiological
examination —
Part 6:
Specific rules for the preparation
of samples taken at the primary
production stage
Microbiologie des aliments — Préparation des échantillons, de
la suspension mère et des dilutions décimales en vue de l’examen
microbiologique —
Partie 6: Règles spécifiques pour la préparation des échantillons
prélevés au stade de production primaire
Reference number
ISO 6887-6:2013(E)
ISO 2013
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SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2013

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form

or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior

written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of

the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2013 – All rights reserved
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SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 2

5 Diluents and disinfectants .......................................................................................................................................................................... 2

5.1 Diluents for special purposes ..................................................................................................................................................... 2

5.2 Disinfectants for use during laboratory examination ........................................................................................... 3

6 Apparatus and glassware ............................................................................................................................................................................ 3

7 Types of sample that can be sent to the laboratory ......................................................................................................... 3

8 Preparation of samples.................................................................................................................................................................................. 3

8.1 General ........................................................................................................................................................................................................... 3

8.2 Storage ........................................................................................................................................................................................................... 4

9 Specific procedures............................................................................................................................................................................................ 4

9.1 Procedures carried out on samples taken at farm ................................................................................................... 4

9.2 Procedures carried out on samples taken from slaughterhouse ................................................................. 6

9.3 Procedures for samples taken from poultry at hatchery or when transporting from

hatchery to farm .................................................................................................................................................................................... 7

10 Further decimal dilutions ........................................................................................................................................................................... 8

Bibliography ................................................................................................................................................................................................................................ 9

© ISO 2013 – All rights reserved iii
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SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.

The main task of technical committees is to prepare International Standards. Draft International

Standards adopted by the technical committees are circulated to the member bodies for voting.

Publication as an International Standard requires approval by at least 75 % of the member bodies

casting a vote.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 6887-6 was prepared by the European Committee for Standardization (CEN) Technical Committee

CEN/TC 275, Food analysis — Horizontal methods, in collaboration with Technical Committee ISO/TC 34,

Food products, Subcommittee SC 9, Microbiology, in accordance with the Agreement on technical

cooperation between ISO and CEN (Vienna Agreement).

ISO 6887 consists of the following parts, under the general title Microbiology of food and animal feed —

Preparation of test samples, initial suspension and decimal dilutions for microbiological examination:

— Part 1: General rules for the preparation of the initial suspension and decimal dilutions

— Part 2: Specific rules for the preparation of meat and meat products
— Part 3: Specific rules for the preparation of fish and fishery products

— Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat

products, and fish and fishery products
— Part 5: Specific rules for the preparation of milk and milk products

— Part 6: Specific rules for the preparation of samples taken at the primary production stage

1) It is intended that, upon revision, the main element of Parts 2 to 5 will be aligned with the main element of the

title of Part 6 (i.e. “Microbiology of food and animal feed”).
iv © ISO 2013 – All rights reserved
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SIST EN ISO 6887-6:2013
INTERNATIONAL STANDARD ISO 6887-6:2013(E)
Microbiology of food and animal feed — Preparation of
test samples, initial suspension and decimal dilutions for
microbiological examination —
Part 6:
Specific rules for the preparation of samples taken at the
primary production stage
1 Scope

This part of ISO 6887 specifies rules for the preparation of samples taken at all stages from the farm

to the slaughterhouse and their suspension for microbiological examination when the samples require

different preparation from the methods described in ISO 6887-1. ISO 6887-1 defines the general rules for

the preparation of the initial suspension and decimal dilutions for microbiological examination.

This part of ISO 6887 excludes the preparation of samples for both enumeration and detection test

methods where preparation details are specified in the relevant International Standards.

This part of ISO 6887 is applicable to various samples taken from the hatchery, the farm, from the

vehicle or the animals during transportation, or from animals or their carcasses in the slaughterhouse,

to indicate the microbiological status of the animals in relation to zoonotic agents. This part of ISO 6887

does not apply to samples taken to assess the hygiene of meat. These are covered by ISO 6887-2.

This part of ISO 6887 does not consider samples taken from the aquatic environment (marine or

freshwater) at the primary production stage. These are covered by ISO 6887-3.
2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated

references, the latest edition of the referenced document (including any amendments) applies.

ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension

and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the

initial suspension and decimal dilutions

ISO 7218:2007, Microbiology of food and animal feeding stuffs — General requirements and guidance for

microbiological examinations

ISO 13307, Microbiology of food and animal feed — Primary production stage — Sampling techniques

3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
laboratory sample

sample prepared for sending to the laboratory and intended for inspection or testing

© ISO 2013 – All rights reserved 1
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SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)
3.2
test portion

measured (volume or mass) representative sample taken from the laboratory sample (3.1) for use in the

preparation of the initial suspension (3.4)
3.3
pooled sample

composite sample taken from a number of different animals or composites of separate environmental

samples
3.4
initial suspension
primary dilution

suspension, solution or emulsion obtained after a weighed or measured quantity of the product under

examination (or of a test sample prepared from the product) has been mixed with, normally, a nine-fold

quantity of diluent, allowing large particles, if present, to settle

Note 1 to entry: Sometimes higher or lower initial dilutions are prepared, for example 1 in 5 or 1 in 100 (V → V ).

1 2
3.5
further decimal dilutions

suspension or solution obtained by mixing a measured volume of the initial suspension (3.4) with a nine-

fold volum
...

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