EN ISO 11816-2:2024

Milk and milk products - Determination of alkaline phosphatase activity - Part 2: Fluorimetric method for cheese (ISO 11816-2:2024)

This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in cheese.
This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7).
The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits per litre (mU/kg).

Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil 2: Fluorimetrisches Verfahren für Käse (ISO 11816-2:2024)

Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2: Méthode fluorimétrique pour le fromage (ISO 11816-2:2024)

Le présent document spécifie une méthode fluorimétrique de détermination de l’activité de la phosphatase alcaline (ALP) (EC 3.1.3.1) dans le fromage.
La présente méthode est applicable aux fromages à pâte molle, pâte pressée non cuite et pâte pressée cuite, à condition que la moisissure ne soit présente que sur la surface du fromage et ne se soit pas propagée à l’intérieur, comme dans le cas des fromages veinés de bleu, par exemple. Pour les gros fromages à pâte pressée cuite, des conditions d’échantillonnage spécifiques s’appliquent (voir l’Article 7).
L’instrument utilisé pour la détermination de l’ALP peut lire des activités jusqu’à 7 000 mU/kg (milliunités par kilogramme).

Mleko in mlečni izdelki - Določanje aktivnosti alkalne fosfataze - 2. del: Fluorometrijska metoda za sir (ISO 11816-2:2024)

Ta dokument določa fluorimetrijsko metodo za določanje aktivnosti alkalne fosfataze (ALP)
(EC 3.1.3.1) v siru.
Ta metoda se uporablja za topljene, poltrde in trde sire pod pogojem, da je plesen samo na površini sira, in ne tudi na notranji strani (npr. sir z modrimi plesnimi). Za velike trde sire veljajo posebni pogoji vzorčenja (glej točko 7).
Instrument, ki se uporablja za določanje alkalne fosfataze, lahko beleži aktivnosti v supernatantu do 7000 milienot na liter (mU/kg).

General Information

• Status: Published
• Publication Date: 16-Jan-2024
• ICS: 67.100.30 - Cheese
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 17-Jan-2024
• Completion Date: 17-Jan-2024
• Ref Project: SIST EN ISO 11816-2:2024 - Milk and milk products - Determination of alkaline phosphatase activity - Part 2: Fluorimetric method for cheese (ISO 11816-2:2024)

Relations

• Replaces: EN ISO 11816-2:2016 - Milk and milk products - Determination of alkaline phosphatase activity - Part 2: Fluorimetric method for cheese (ISO 11816-2:2016)
• Effective Date: 18-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-marec-2024
Nadomešča:
SIST EN ISO 11816-2:2016
Mleko in mlečni proizvodi - Določanje aktivnosti alkalne fosfataze - 2. del:
Fluorometrijska metoda za sir (ISO 11816-2:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 2:
Fluorimetric method for cheese (ISO 11816-2:2024)
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil
2: Fluorimetrisches Verfahren für Käse (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2:
Méthode fluorimétrique pour le fromage (ISO 11816-2:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-2:2024
ICS:
67.100.30 Sir Cheese
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11816-2
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2024
EUROPÄISCHE NORM
ICS 67.100.30 Supersedes EN ISO 11816-2:2016
English Version
Milk and milk products - Determination of alkaline
phosphatase activity - Part 2: Fluorimetric method for
cheese (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milchprodukte - Bestimmung der Aktivität
la phosphatase alcaline - Partie 2: Méthode der alkalischen Phosphatase - Teil 2: Fluorimetrisches
fluorimétrique pour le fromage (ISO 11816-2:2024) Verfahren für Käse (ISO 11816-2:2024)
This European Standard was approved by CEN on 29 March 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-2:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11816-2:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-2:2016.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 11816-2:2024 has been approved by CEN as EN ISO 11816-2:2024 without any
modification.
International
Standard
ISO 11816-2
IDF 155-2
Third edition
Milk and milk products —
2024-01
Determination of alkaline
phosphatase activity —
Part 2:
Fluorimetric method for cheese
Lait et produits laitiers — Détermination de l'activité de la
phosphatase alcaline —
Partie 2: Méthode fluorimétrique pour le fromage
Reference numbers
ISO 11816-2:2024(en)
IDF 155-2:2024(en) © ISO and IDF 2024

ISO 11816-2:2024(en)
IDF 155-2:2024(en)
© ISO and IDF 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: [phone] Phone: [phone]
Fax: [phone]
Email: [e-mail] Email: [e-mail]
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
ISO 11816-2:2024(en)
IDF 155-2:2024(en)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure . 5
9.1 Verification of instrument performance .5
9.1.1 General .5
9.1.2 Daily instrument tests .5
9.1.3 Using FLM200 .5
9.1.4 Using FLM300 .5
9.1.5 Controls .6
9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3) .6
9.3 Calibration .6
9.3.1 General .6
9.3.2 Using FLM200 .7
9.3.3 Using FLM300 .7
9.4 Determination .7
9.5 Test-sample-related controls .8
9.5.1 Recommended negative and positive control tests .8
9.5.2 Interfering substance test .9
9.5.3 Heat-stable microbial ALP control test .9
10 Calculation and expression of results . 9
10.1 Calibration ratio .9
10.2 Calculation .10
10.2.1 Supernatant .10
10.2.2 Cheese .10
10.3 Expression of test results.10
11 Precision .11
11.1 Interlaboratory study .11
11.2 Repeatability .11
11.3 Reproducibility .11
12 Test report .11
Annex A (informative) Interlaboratory study .12
Annex B (informative) Examples of preparation of a test sample .
...

SLOVENSKI STANDARD
01-marec-2024
Nadomešča:
SIST EN ISO 11816-2:2016
Mleko in mlečni izdelki - Določanje aktivnosti alkalne fosfataze - 2. del:
Fluorometrijska metoda za sir (ISO 11816-2:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 2:
Fluorimetric method for cheese (ISO 11816-2:2024)
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil
2: Fluorimetrisches Verfahren für Käse (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 2:
Méthode fluorimétrique pour le fromage (ISO 11816-2:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-2:2024
ICS:
67.100.30 Sir Cheese
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11816-2
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2024
EUROPÄISCHE NORM
ICS 67.100.30 Supersedes EN ISO 11816-2:2016
English Version
Milk and milk products - Determination of alkaline
phosphatase activity - Part 2: Fluorimetric method for
cheese (ISO 11816-2:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milchprodukte - Bestimmung der Aktivität
la phosphatase alcaline - Partie 2: Méthode der alkalischen Phosphatase - Teil 2: Fluorimetrisches
fluorimétrique pour le fromage (ISO 11816-2:2024) Verfahren für Käse (ISO 11816-2:2024)
This European Standard was approved by CEN on 29 March 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-2:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11816-2:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-2:2016.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 11816-2:2024 has been approved by CEN as EN ISO 11816-2:2024 without any
modification.
International
Standard
ISO 11816-2
IDF 155-2
Third edition
Milk and milk products —
2024-01
Determination of alkaline
phosphatase activity —
Part 2:
Fluorimetric method for cheese
Lait et produits laitiers — Détermination de l'activité de la
phosphatase alcaline —
Partie 2: Méthode fluorimétrique pour le fromage
Reference numbers
ISO 11816-2:2024(en)
IDF 155-2:2024(en) © ISO and IDF 2024

ISO 11816-2:2024(en)
IDF 155-2:2024(en)
© ISO and IDF 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: [phone] Phone: [phone]
Fax: [phone]
Email: [e-mail] Email: [e-mail]
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
ISO 11816-2:2024(en)
IDF 155-2:2024(en)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure . 5
9.1 Verification of instrument performance .5
9.1.1 General .5
9.1.2 Daily instrument tests .5
9.1.3 Using FLM200 .5
9.1.4 Using FLM300 .5
9.1.5 Controls .6
9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3) .6
9.3 Calibration .6
9.3.1 General .6
9.3.2 Using FLM200 .7
9.3.3 Using FLM300 .7
9.4 Determination .7
9.5 Test-sample-related controls .8
9.5.1 Recommended negative and positive control tests .8
9.5.2 Interfering substance test .9
9.5.3 Heat-stable microbial ALP control test .9
10 Calculation and expression of results . 9
10.1 Calibration ratio .9
10.2 Calculation .10
10.2.1 Supernatant .10
10.2.2 Cheese .10
10.3 Expression of test results.10
11 Precision .11
11.1 Interlaboratory study .11
11.2 Repeatability .11
11.3 Reproducibility .11
12 Test report .11
Annex A (informative) Interlaboratory study .12
Annex B (informative) Examples of preparation of a test sample .
...