EN 15850:2010
(Main)Foodstuffs - Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection
Foodstuffs - Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection
This European Standard specifies a method for the determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of maize based baby food, barley flour, maize flour, polenta and wheat flour ranging from 10 µg/kg to 335 µg/kg, and the second study was for samples of cereal based foods for infants and young children ranging from 9 µg/kg to 44 µg/kg.
Lebensmittel - Bestimmung von Zearalenon in Säuglingsnahrung auf Maisbasis, Gerstenmehl, Maismehl, Maisgrieß, Weizenmehl und Lebensmittel auf Getreidebasis für Säuglinge und Kleinkinder - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion
Diese Europäische Norm legt ein Verfahren zur Bestimmung von Zearalenon in Säuglingsnahrung auf Maisbasis, Gerstenmehl, Maismehl, Maisgrieß, Weizenmehl und Lebensmittel auf Getreidebasis für Säuglinge und Kleinkinder durch Hochleistungsflüssigchromatographie (HPLC) mit Reinigung an einer Immunoaffinitäts-säule und Fluoreszenzdetektion fest. Dieses Verfahren wurde in zwei Ringversuchen validiert. Der erste Ringversuch wurde für die Analyse von Säuglingsnahrung auf Maisbasis, Gerstenmehl, Maismehl, Maisgrieß und Weizenmehl mit Gehalten von 10 µg/kg bis 335 µg/kg und der zweite Ringversuch für Säuglings- und Kleinkindernahrung auf Getreidebasis mit Gehalten von 9 µg/kg bis 44 µg/kg durchgeführt.
Weitere Informationen zur Validierung befinden sich in Abschnitt 9 und Anhang B.
Produits alimentaires - Dosage de la zéaralénone dans les aliments à base de maïs pour bébés, dans la farine d'orge, dans la farine de maïs, dans la polenta, dans la farine de blé et dans les aliments à base de céréales pour nourrissons et enfants en bas âge - Méthode par chromatographie liquide haute performance avec purification sur colonne d'immuno-affinité et détection par fluorescence
La présente Norme européenne spécifie une méthode pour déterminer la teneur en zéaralénone dans les aliments
à base de maïs pour bébés, dans la farine d’orge, dans la farine de maïs, dans la polenta, dans la farine de blé et
dans les aliments à base de céréales pour nourrissons et enfants en bas âge, par chromatographie liquide haute
performance (CLHP) avec purification sur colonne d’immuno-affinité et détection fluorimétrique. La présente
méthode a été validée dans deux études interlaboratoires, la première portant sur l’analyse d’échantillons
d’aliments à base de maïs pour bébés, de farine d’orge, de farine de maïs, de polenta et de farine de blé, à des
concentrations allant de 10 μg/kg à 335 μg/kg et la seconde sur l’analyse d’échantillons d’aliments à base de
céréales pour nourrissons et enfants en bas âge à des concentrations allant de 9 μg/kg à 44 μg/kg.
Pour plus d’informations sur la validation, voir l’Article 9 et l’Annexe B.
Živila - Določevanje zearalenona v hrani za dojenčke in majhne otroke na osnovi koruze, ječmenove moke, koruzne moke, polente, pšenične moke ter v žitnih kašicah - Metoda HPLC z imunoafinitetnim kolonskim čiščenjem in fluorescenčno detekcijo
Ta evropski standard določa metodo za določevanje zearalenona v hrani za dojenčke na osnovi koruze, ječmenove moke, koruzne moke, polente, pšenične moke in žitnih kašicah za dojenčke in majhne otroke z tekočinsko kromatografijo visoke ločljivosti (HPLC) z imunoafinitetnim čiščenjem in fluorescenčno detekcijo. Ta metoda je bila potrjena v dveh medlaboratorijskih raziskavah. Prva raziskava je bila izvedena za analizo vzorcev hrane za dojenčke na osnovi koruze, ječmenove moke, koruzne moke, polente in pšenične moke v razponu od 10 µg/kg do 335 µg/kg, druga raziskava pa je bila za vzorce hrane za dojenčke in majhne otroke na osnovi žit v razponu od 9 µg/kg do 44 µg/kg. Za dodatne informacije, glej Klavzulo 9 in Dodatek B.
General Information
Overview
EN 15850:2010 (CEN) specifies a validated laboratory method for the determination of zearalenone in cereal products intended for infants and young children - including maize based baby food, barley flour, maize flour, polenta and wheat flour. The method uses high performance liquid chromatography (HPLC) with immunoaffinity column cleanup followed by fluorescence detection. It has been validated in two interlaboratory studies across concentration ranges from about 9 µg/kg to 335 µg/kg.
Key topics and requirements
- Analytical principle: Extraction with aqueous acetonitrile or methanol, dilution with phosphate buffered saline (PBS), cleanup using an immunoaffinity column (IAC) specific for zearalenone, elution with organic solvent, and quantification by reverse-phase HPLC with fluorescence detection.
- Validated ranges: Interlaboratory validation covered samples from ~10 µg/kg to 335 µg/kg (grains/flours) and ~9 µg/kg to 44 µg/kg (cereal-based infant foods).
- Immunoaffinity column: IAC must contain antibodies specific to zearalenone, with a capacity ≥ 1 500 ng and recovery ≥ 80 % when 75 ng is applied under specified conditions.
- Reagents and mobile phases: Solvent recipes are specified (e.g., extraction solvent A: 75% acetonitrile / 25% water; extraction solvent B: 75% methanol / 25% water; mobile phases and injection solvents also defined).
- HPLC detection: Fluorescence excitation ~274–275 nm and emission ~446–450 nm; recommended reverse‑phase columns and injection volumes are provided (examples given, not endorsements).
- Apparatus & safety: Lists required lab equipment (homogenizer, vacuum manifold, syringe filters, HPLC system). Warnings for handling acetonitrile and the oestrogenic compound zearalenone (use fume hood, gloves, eye protection).
- Documentation: Calculation, precision data (Annex B), typical chromatograms (Annex A) and test reporting requirements are included.
Practical applications
- Routine monitoring and compliance testing of infant and young‑child cereal foods for mycotoxin (zearalenone) contamination.
- Quality control in cereal and baby‑food manufacturing supply chains.
- Regulatory surveillance by food safety authorities and reference laboratories.
- Research studies on mycotoxin occurrence, exposure assessment, and mitigation strategies.
Who should use this standard
- Food testing laboratories and contract analytical services
- National and regional food safety/regulatory agencies
- Baby food and cereal manufacturers’ quality assurance teams
- Research institutions studying mycotoxins and infant food safety
Related standards
- EN ISO 3696 (water for analytical laboratory use) is cited for water quality used in reagents and mobile phases.
Keywords: EN 15850:2010, zearalenone, HPLC, immunoaffinity column, fluorescence detection, maize based baby food, cereal based foods for infants and young children, barley flour, maize flour, polenta, wheat flour.
Frequently Asked Questions
EN 15850:2010 is a standard published by the European Committee for Standardization (CEN). Its full title is "Foodstuffs - Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection". This standard covers: This European Standard specifies a method for the determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of maize based baby food, barley flour, maize flour, polenta and wheat flour ranging from 10 µg/kg to 335 µg/kg, and the second study was for samples of cereal based foods for infants and young children ranging from 9 µg/kg to 44 µg/kg.
This European Standard specifies a method for the determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of maize based baby food, barley flour, maize flour, polenta and wheat flour ranging from 10 µg/kg to 335 µg/kg, and the second study was for samples of cereal based foods for infants and young children ranging from 9 µg/kg to 44 µg/kg.
EN 15850:2010 is classified under the following ICS (International Classification for Standards) categories: 67.060 - Cereals, pulses and derived products. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 15850:2010 is associated with the following European legislation: Standardization Mandates: M/383. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
You can purchase EN 15850:2010 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.OXRUHVFHQþQRGHWHNFLMRLebensmittel - Bestimmung von Zearalenon in Säuglingsnahrung auf Maisbasis, Gerstenmehl, Maismehl, Maisgrieß, Weizenmehl und Lebensmittel auf Getreidebasis für Säuglinge und Kleinkinder - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und FluoreszenzdetektionProduits alimentaires - Dosage de la zéaralénone dans les aliments à base de maïs pour bébés, dans la farine d'orge, dans la farine de maïs, dans la polenta, dans la farine de blé et dans les aliments à base de céréales pour nourrissons et enfants en bas âge - Méthode par chromatographie liquide haute performance avec purification sur colonne d'immuno-affinité et détection par fluorescenceFoodstuffs - Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection67.230Predpakirana in pripravljena hranaPrepackaged and prepared foods67.060QMLKCereals, pulses and derived productsICS:Ta slovenski standard je istoveten z:EN 15850:2010SIST EN 15850:2010en,fr,de01-september-2010SIST EN 15850:2010SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15850
April 2010 ICS 67.060 English Version
Foodstuffs - Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection
Produits alimentaires - Dosage de la zéaralénone dans la farine d'orge, de maïs et de blé, la polenta et les produits pour nourrissons et jeunes enfants à base de céréales - Méthode par chromatographie liquide haute performance avec purification sur colonne d'immunoaffinité et détection par fluorescence
Lebensmittel - Bestimmung von Zearalenon in Säuglingsnahrung auf Maisbasis, Gerstenmehl, Maismehl, Maisgrieß, Weizenmehl und Lebensmittel auf Getreidebasis für Säuglinge und Kleinkinder - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion This European Standard was approved by CEN on 27 February 2010.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2010 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15850:2010: ESIST EN 15850:2010
Typical chromatograms . 15Annex B (informative)
Precision data . 16Bibliography . 18 SIST EN 15850:2010
Specification and test methods (ISO 3696:1987). 3 Principle A test portion is extracted with aqueous acetonitrile or methanol according to the products analyzed. The extract is then diluted with phosphate buffered saline (PBS) to give an aqueous extract that is applied to an immunoaffinity column containing antibodies specific for zearalenone. Zearalenone is purified and concentrated on the column and removed from the antibodies using acetonitrile or methanol as eluent. Zearalenone is quantified by reverse-phase high performance liquid chromatography (RP-HPLC) with fluorescence detection. 4 Reagents 4.1 General Use only reagents of recognised analytical grade and water complying with grade 1 of EN ISO 3696:1995, unless otherwise specified. Solvents shall be of quality for HPLC analysis, unless otherwise specified. Commercially available solutions with equivalent properties to those listed may be used. 4.2 Disodium hydrogen phosphate, Na2HPO4 anhydrous or Na2HPO4·12 H2O. 4.3 Potassium chloride (KCl). 4.4 Potassium dihydrogen phosphate, KH2PO4. 4.5 Sodium chloride (NaCl). 4.6 Sodium hydroxide (NaOH). 4.7 Hydrochloric acid solution, mass fraction w(HCl) = 37 % in water. 4.8 Hydrochloric acid solution, substance concentration c(HCl) = 0,1 mol/l. Dilute 8,28 ml of hydrochloric acid solution (4.7) to 1 l with water. SIST EN 15850:2010
technical grade. 4.14 Extraction solvent A. Mix 75 parts per volume of acetonitrile (4.11) with 25 parts per volume of water. 4.15 Injection solvent A for HPLC analysis. Mix four parts per volume of acetonitrile (4.11) with six parts per volume of water. 4.16 HPLC mobile phase A. Mix 53 parts per volume of acetonitrile (4.11) with 47 parts per volume of water. Filter and degas the HPLC mobile phase before use. 4.17 Extraction solvent B. Mix 75 parts per volume of methanol (4.13) with 25 parts per volume of water. 4.18 Washing solvent. Mix 15 parts per volume of methanol (4.12) with 85 parts per volume of PBS (4.10). 4.19 Injection solvent B for HPLC analysis. Mix five parts per volume of methanol (4.12) with five parts per volume of water. 4.20 HPLC mobile phase B. Mix 75 parts per volume of methanol (4.12) with 25 parts per volume of water. Filter and degas the HPLC mobile phase before use. SIST EN 15850:2010
bMA×××=ερ100maxzon (1) where Amax is the absorption determined at the maximum of the absorption curve (274 nm); M is the molar mass, in grams per mole, of zearalenone (M = 318,4 g/mol); 0 is the molar absorption coefficient, in square metres per mole of zearalenone in acetonitrile (4.11) ( 1 262 m2/mol, see [1]); b is the optical path length, in centimetres, of the quartz cell. Store this solution in a freezer at - 18 °C to - 20 °C. Allow to reach room temperature before opening. A solution stored in this way is usually stable for 12 months. Confirm the mass concentration of the solution if it is older than six months. 4.25 Zearalenone standard solution A,
= 2 µg/ml, for maize based baby food, barley flour, maize flour, polenta and wheat flour. Transfer an aliquot of the spiking solution (4.24) equivalent to 10 µg of zearalenone into either a vial (5.9) or a calibrated volumetric flask (5.10). Add acetonitrile (4.11) to make the total volume up to 5 ml.
= 0,4 µg/ml, for cereal based foods for infants and young children. Transfer an aliquot of the spiking solution (4.24) equivalent to 2 µg of zearalenone into either a vial (5.9) or a calibrated volumetric flask (5.10). Add acetonitrile (4.11) to make the total volume up to 5 ml.
Store this solution in a freezer at - 18 °C to - 20 °C. Allow to reach room temperature before opening. A solution stored in this way is usually stable for 12 months. Confirm the mass concentration of the solution if it is older than six months. 5 Apparatus 5.1 General Usual laboratory glassware and equipment and, in particular, the following. 5.2 High speed blender or homogenizer. 5.3 Analytical balance, capable of weighing to 0,000 1 g. 5.4 Laboratory balance, capable of weighing to 0,1 g. 5.5 Adjustable vertical or horizontal shaker. 5.6 Vortex mixer, or equivalent. 5.7 Mills, various screens. 5.8 Tumble mixer. 5.9 Glass vials, of various sizes 5.10 Volumetric flasks, of 3 ml or 5 ml and 10 ml capacity. 5.11 Beaker, of 250 ml capacity. 5.12 Conical flask, with screw cap or glass stopper of 100 ml, 250 ml and 500 ml capacity. 5.13 Filter paper, e.g., qualitative, strong, fast flow, 24 cm diameter, 30 µm pore size, prefolded or equivalent. 5.14 Glass microfibre filter, e.g. 1,6 µm retention size or equivalent. 5.15 Pipettes, of e.g. 25 µl to 250 µl, 1 ml, 5 ml and 10 ml capacity. 5.16 Displacement micropipettes or syringes, gas tight of e.g. 100 µl, 500 µl, 1 000 µl capacity. SIST EN 15850:2010
Phenomenex Prodigy® ODS 3 1) (150 mm × 4,6 mm internal diameter, 5 µm particle size, 25 nm pore size) or Spherisorb® 1) ODS-2 Excel (250 mm × 4,6 mm internal diameter, 5 µm particle size, 25 nm pore size) have been found to be suitable when used with mobile phase A (4.16). Supelcosil® 1) (C18), fully end capped (250 mm × 4,6 mm internal diameter, 5 µm particle size, 18 nm pore size), carbon loading of 12 %, or similar, has been found to be suitable when used with mobile phase B (4.20). 5.24.4 Pre-column, with preferably the same stationary phase material as the analytical column, an internal diameter of 4 mm, 5 µm particle size. 5.24.5 Fluorescence detector, fitted with a flow cell and suitable for measurements with excitation wavelength of 274 nm or 275 nm and emission at 446 nm or 450 nm. 5
...
EN 15850:2010は、乳幼児向けの食品や穀物製品に含まれるゼアラレノンの測定方法を規定している非常に重要な標準であり、特にトウモロコシベースのベビーフード、大麦粉、トウモロコシ粉、ポレンタ、小麦粉、穀物ベースの食品に関するものです。この標準では、高性能液体クロマトグラフィー（HPLC）を用いた免疫親和性クリーンアップおよび蛍光検出による高精度なゼアラレノンの定量を実現しています。 標準の大きな強みは、2回のインターレボラトリースタディにおいてその手法が検証されている点です。これにより、トウモロコシベースのベビーフードや小麦粉など複数の試料に対して、10 µg/kgから335 µg/kgの範囲での分析が実施され、乳幼児向けの穀物ベース食品についても9 µg/kgから44 µg/kgの範囲での信頼性のある結果が得られています。この検証により、業界が求める高い安全基準を維持できることが保障されています。 EN 15850:2010の関連性は、食品業界において危害分析重要点（HACCP）や食品安全規制がますます重要視される中で、ゼアラレノンの測定が乳幼児や幼児の健康を守るための重要な要素となることにあります。この標準に従うことで、製品の品質管理が強化され、消費者に対する信頼の向上が図られます。
La norme EN 15850:2010 se distingue par son approche rigoureuse pour la détermination de la zéaralénone dans les aliments pour bébés à base de maïs, la farine d’orge, la farine de maïs, la polenta, la farine de blé et les aliments céréaliers destinés aux nourrissons et jeunes enfants. Ce document normatif, spécifiquement conçu pour les professionnels de l'alimentation, établit une méthode claire utilisant la chromatographie liquide de haute performance (HPLC) combinée à un nettoyage par immunoaffinité et à une détection par fluorescence. L’un des points forts de cette norme est sa validation s'appuyant sur deux études interlaboratoires. La première étude a porté sur des échantillons de nourriture pour bébés à base de maïs, de farine d’orge, de farine de maïs, de polenta et de farine de blé, avec des niveaux de zéaralénone allant de 10 µg/kg à 335 µg/kg. La seconde étude, quant à elle, a examiné des échantillons d'aliments céréaliers pour bébés, avec des niveaux mesurés entre 9 µg/kg et 44 µg/kg. Ces validations garantissent la fiabilité et la reproductibilité des résultats obtenus. En termes de pertinence, la norme EN 15850:2010 est cruciale dans le contexte actuel où la sécurité alimentaire est une préoccupation majeure. Elle permet d’assurer que les produits destinés aux nourrissons et jeunes enfants répondent aux exigences élevées de sécurité, minimisant ainsi les risques d’exposition à la zéaralénone, un contaminant potentiel. En conclusion, la norme représente un outil indispensable pour les laboratoires d'analyse et les producteurs, favorisant la confiance dans la conformité des aliments pour bébés et jeunes enfants. La méthode HPLC avec nettoyage par immunoaffinité et détection par fluorescence offre une solution efficace et précise pour le contrôle de ce contaminant dans les produits céréaliers.
이 표준 문서는 EN 15850:2010으로, 아기식, 보리 가루, 옥수수 가루, 폴렌타, 밀가루 및 유아용 시리얼 기반 식품에서 제라알렌온(zearalenone)을 측정하는 방법을 제시합니다. 표준의 범위는 고성능 액체 크로마토그래피(HPLC)와 면역 친화성 정화 및 형광 검출을 이용하여 식품 내 제라알렌온의 정량 분석 방법을 포함하고 있습니다. 이 표준의 강점 중 하나는 두 차례의 인터라보라토리 연구를 통해 검증되었다는 점입니다. 첫 번째 연구는 옥수수 기반 아기식과 보리 가루, 옥수수 가루, 폴렌타 및 밀가루 샘플을 대상으로 하였으며, 분석 대상 농도는 10 µg/kg에서 335 µg/kg까지 다양합니다. 두 번째 연구는 유아용 시리얼 기반 식품에 대해 9 µg/kg에서 44 µg/kg 농도의 샘플을 분석했습니다. 이러한 연구는 표준의 신뢰성을 높이며, 다양한 식품에서 제라알렌온의 정확한 검출 및 정량을 가능하게 합니다. 이 문서는 매우 중요한 식품 안전 기준으로, 아기와 어린이의 건강을 보호하는 데 필요한 데이터를 제공하여 식품 산업의 품질 확보에 기여합니다. 표준의 적용은 특히 유아 및 어린이를 위한 제품에서 제라알렌온의 안전성을 보장하는데 필수적입니다. EN 15850:2010은 유럽 내에서 법적 요구 사항과 규정 준수를 위해 식품 생태계에서 필수적으로 다루어져야 할 문서입니다.
The standard EN 15850:2010 provides a comprehensive and systematic approach for the determination of zearalenone in specific food materials, including maize-based baby food, barley flour, maize flour, polenta, wheat flour, and cereal-based foods tailored for infants and young children. The specified method utilizes high-performance liquid chromatography (HPLC) combined with an immunoaffinity column cleanup and fluorescence detection, ensuring both sensitivity and reliability in detecting zearalenone levels in these vital food products. A notable strength of this standard lies in its dual validation through interlaboratory studies, which enhances its credibility and applicability across different testing environments. The validated analytical range-from 10 µg/kg to 335 µg/kg for certain food samples and 9 µg/kg to 44 µg/kg for infant foods-provides laboratories with precise parameters for quantifying zearalenone. This ensures that manufacturers and regulators can maintain food safety standards effectively, especially concerning vulnerable populations such as infants and young children. The relevance of EN 15850:2010 cannot be overstated, as it addresses critical safety concerns regarding mycotoxin contamination in food products designed for children. By establishing a clear methodology for zearalenone analysis, this standard supports public health initiatives aimed at safeguarding the nutritional quality of infant and young children’s foods. The emphasis on high-performance liquid chromatography (HPLC) in the standard further highlights its alignment with modern analytical practices, making it an essential tool for food safety laboratories. In summary, EN 15850:2010 serves as a vital regulatory benchmark that combines rigorous scientific validation with practical application in food safety, ensuring that producers can effectively monitor zearalenone levels in their products.
Die Norm EN 15850:2010 bietet eine umfassende und präzise Methode zur Bestimmung von Zearalenon in verschiedenen Lebensmitteln, die sowohl für Säuglinge als auch für Kleinkinder geeignet sind. Mit dem Fokus auf maize basierte Babynahrung, Gerstenmehl, Maismehl, Polenta, Weizenmehl und Getreideprodukte ermöglicht diese europäische Norm den Herstellern, die Sicherheit und Qualität ihrer Produkte zu gewährleisten. Ein herausragendes Merkmal der Norm ist die Verwendung von Hochleistungsflüssigkeitschromatographie (HPLC) in Kombination mit Immunaffinitätsreinigen und Fluoreszenzdetektion. Diese Technik ermöglicht eine äußerst empfindliche und spezifische Analyse des Zearalenon-Gehalts, was äußerst wichtig ist, da Zearalenon in hohen Konzentrationen gesundheitsschädlich sein kann, insbesondere für Kleinkinder. Die Validierung dieses Verfahrens durch zwei Zwischenlaborstudien unterstreicht die Zuverlässigkeit und Reproduzierbarkeit der Methode. Die erste Studie konzentrierte sich auf diverse Proben von maize basierter Babynahrung sowie verschiedenen Mehlen, während die zweite Studie speziell auf Getreideprodukte für Säuglinge und Kleinkinder abzielte. Die untersuchten Gehalte von Zearalenon, die zwischen 9 µg/kg und 335 µg/kg variieren, decken ein breites Spektrum ab, wodurch die Norm für Hersteller und Labore relevant ist, die eine genaue Überwachung von Kontaminanten in ihren Produkten sicherstellen möchten. Insgesamt ist die EN 15850:2010 eine wesentliche Norm für die Lebensmittelindustrie, welche die gesundheitliche Unbedenklichkeit von Produkten für Säuglinge und Kleinkinder maßgeblich unterstützt. Ihre klaren Vorgaben und die verlässlichen Analysemethoden machen sie zu einem unverzichtbaren Werkzeug für die Qualitätssicherung im Bereich der baby- und kleinkindgerechten Lebensmittel.
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.
Loading comments...