EN 13585:2001

Foodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up

This European Standard specifies a method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in maize using high performance liquid chromatography (HPLC).
The method has been successfully validated in an interlaboratory study according to AOAC Guidelines for Collaborative Studies [1] on maize containing 405 µg/kg to 6732 mg/kg fumonisin B1 and 152 mg/kg to 2619 mg/kg fumonisin B2. The method works well with maize or minimally processed maize (e.g. fresh, dried and milled maize), but does not provide reliable results with most maize-based processed products.

Lebensmittel - Bestimmung von Fumonisin B1 und B2 in Mais - HPLC-Verfahren mit Reinigung durch Festphasenextraktion

Dieser Europäische Norm-Entwurf legt ein hochleistungs-flüssigchromatographisches (HPLC)-Verfahren für die Bestimmung von Fumonisin B1 (FB1) und Fumonisin B2 (FB2) in Mais fest. Das Verfahren wurde erfolgreich in einem Ringversuch nach ISO 5725: 1986 an Mais, der 405 Mikron g/kg bis 6732 Mikron g/kg Fumonisin B1 und 152 Mikron g/kg bis 2619 Mikron g/kg Fumonisin B2 enthält, validiert.

Produits alimentaires - Dosage des fumonisines B1 et B2 dans le maïs - Méthode CLHP avec purification par extraction en phase solide

La présente Norme européenne spécifie une méthode de dosage de la fumonisine B1 (FB1) et de la fumonisine B2 (FB2) dans le maïs, par chromatographie en phase liquide haute performance(CLHP).
Cette méthode a été validée par un essai interlaboratoires selon les Lignes Directrices AOAC pour Essais Interlaboratoires [1] sur du maïs contenant entre 405 µg/kg et 6 732 µg/kg de fumonisine B1 et entre 152 µg/kg et 2 619 µg/kg de fumonisine B2. Cette méthode fonctionne bien avec du maïs ou du maïs peu transformé (par exemple, du maïs moulu, séché ou frais) mais ne donne pas de résultats fiables avec la plupart des produits transformés à base de maïs.

Živila - Določevanje fumonizinov B1 in B2 v koruzi - HPLC metoda z ekstrakcijo na trdno fazo

General Information

• Status: Withdrawn
• Publication Date: 11-Dec-2001
• Withdrawal Date: 19-Dec-2017
• ICS: 67.060 - Cereals, pulses and derived products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 5 - Biotoxins
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 20-Dec-2017
• Ref Project: SIST EN 13585:2002 - Foodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Fumonisin B1 und B2 in Mais - HPLC-Verfahren mit Reinigung durch FestphasenextraktionProduits alimentaires - Dosage des fumonisines B1 et B2 dans le mais - Méthode CLHP avec purification par extraction en phase solideFoodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up67.060QMLKCereals, pulses and derived productsICS:Ta slovenski standard je istoveten z:EN 13585:2001SIST EN 13585:2002en01-junij-2002SIST EN 13585:2002SLOVENSKI
STANDARD



SIST EN 13585:2002



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 13585December 2001ICS 67.060English versionFoodstuffs - Determination of fumonisins B1 and B2 in maize -HPLC method with solid phase extraction clean-upProduits alimentaires - Dosage des fumonisines B1 et B2dans le maïs - Méthode CLHP avec purification parextraction en phase solideLebensmittel - Bestimmung von Fumonisin B1 und B2 inMais - HPLC-Verfahren mit Reinigung durchFestphasenextraktionThis European Standard was approved by CEN on 2 November 2001.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2001 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 13585:2001 ESIST EN 13585:2002



EN 13585:2001 (E)2ContentspageForeword.21Scope.32Normative references.33Principle.34Reagents and materials.35Apparatus.46Sampling.57Preparation of the test sample.58Procedure.69Calculation.610Precision.711Test report.8Annex A (informative)
Typical chromatogram.10Annex B (informative)
Recovery and relative standard deviation.11Annex C (informative)
Precision data.12Bibliography.15ForewordThis European Standard has been prepared by Technical Com
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