EN ISO 16931:2001
(Main)Animal and vegetable fats and oils - Determination of polymerized triglycerides by high-performance size-exclusion chromatography (HPSEC) (ISO 16931:2001)
Animal and vegetable fats and oils - Determination of polymerized triglycerides by high-performance size-exclusion chromatography (HPSEC) (ISO 16931:2001)
Tierische und pflanzliche Fette und Öle - Bestimmung der polymerisierten Triglyceride mit Hochleistungs-Ausschlußchromatographie (ISO 16931:2001)
Diese Internationale Norm legt ein Verfahren mit Hochleistungs-Ausschlusschromatographie zur Bestimmung der Gehalte an polymerisierten Triglyceriden in Ölen und Fetten fest, die mindestens 3 % (bezogen auf die Peakflächen) dieser Polymere enthalten.
Das Verfahren ist auf Frittierfette und auf Fette und Öle anwendbar, die erhitzt wurden. Es kann auch zur Bestimmung von Polymeren in Futterfetten angewendet werden.
ANMERKUNG 1 Polymerisierte Triglyceride (genau genommen dimere und oligomere Triglyceride) werden bei der Erhitzung von Fetten und Ölen gebildet. Folglich erlaubt dieses Verfahren die Beurteilung der thermischen Belastung von Frittierfetten im Verlaufe ihrer Verwendung.
ANMERKUNG 2 Bei der Untersuchung von Futterfetten kann das angewendete Extraktionsverfahren das Ergebnis beeinflussen (siehe ISO 6492).
Corps gras d'origines animale et végétale - Détermination de la teneur en triglycérides polymérisés par chromatographie liquide d'exclusion à haute performance (CLHP d'exclusion) (ISO 16931:2001)
Rastlinske in živalske maščobe in olja - Določevanje polimeriziranih trigliceridov z izključitveno kromatografijo (HPSEC) (ISO 16931:2001)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 16931:2002
01-junij-2002
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHSROLPHUL]LUDQLKWULJOLFHULGRY]
L]NOMXþLWYHQRNURPDWRJUDILMR +36(& ,62
Animal and vegetable fats and oils - Determination of polymerized triglycerides by high-
performance size-exclusion chromatography (HPSEC) (ISO 16931:2001)
Tierische und pflanzliche Fette und Öle - Bestimmung der polymerisierten Triglyceride
mit Hochleistungs-Ausschlußchromatographie (ISO 16931:2001)
Corps gras d'origines animale et végétale - Détermination de la teneur en triglycérides
polymérisés par chromatographie liquide d'exclusion a haute performance (CLHP
d'exclusion) (ISO 16931:2001)
Ta slovenski standard je istoveten z: EN ISO 16931:2001
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 16931:2002 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 16931:2002
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SIST EN ISO 16931:2002
EUROPEAN STANDARD
EN ISO 16931
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2001
ICS 67.200.10
English version
Animal and vegetable fats and oils - Determination of
polymerized triglycerides by high-performance size-exclusion
chromatography (HPSEC) (ISO 16931:2001)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung der
de la teneur en triglycérides polymérisés par polymerisierten Triglyceride mit Hochleistungs-
chromatographie liquide d'exclusion à haute performance Ausschlußchromatographie (ISO 16931:2001)
(CLHP d'exclusion) (ISO 16931:2001)
This European Standard was approved by CEN on 3 September 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16931:2001 E
worldwide for CEN national Members.
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SIST EN ISO 16931:2002
EN ISO 16931:2001 (E)
Foreword
The text of the International Standard ISO 16931:2001 has been prepared by Technical
Committee ISO/TC 34 "Agricultural food products" in collaboration with Technical Committee
CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Methods of
sampling and analysis", the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by April 2002, and conflicting national
standards shall be withdrawn at the latest by April 2002.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
NOTE FROM CMC The foreword is susceptible to be amended on reception of the German language
version. The confirmed or amended foreword, and when appropriate, the normative annex ZA for the
references to international publications with their relevant European publications will be circulated with
the German version.
Endorsement notice
The text of the International Standard ISO 16931:2001 was approved by CEN as a European
Standard without any modification.
2
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SIST EN ISO 16931:2002
INTERNATIONAL ISO
STANDARD 16931
First edition
2001-10-01
Animal and vegetable fats and oils —
Determination of polymerized triglycerides
content by high-performance size-
exclusion chromatography (HPSEC)
Corps gras d'origines animale et végétale — Détermination de la teneur en
triglycérides polymérisés par chromatographie liquide d'exclusion à haute
performance (CLHP d'exclusion)
Reference number
ISO 16931:2001(E)
©
ISO 2001
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SIST EN ISO 16931:2002
ISO 16931:2001(E)
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ii © ISO 2001 – All rights reserved
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SIST EN ISO 16931:2002
ISO 16931:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 16931 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 11, Animal and vegetable fats and oils.
Annex A of this International Standard is for information only.
© ISO 2001 – All rights reserved iii
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SIST EN ISO 16931:2002
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SIST EN ISO 16931:2002
INTERNATIONAL STANDARD ISO 16931:2001(E)
Animal and vegetable fats and oils — Determination of
polymerized triglycerides content by high-performance
size-exclusion chromatography (HPSEC)
1 Scope
This International Standard specifies a method using HPSEC to determine the contents of polymerized triglycerides
in oils and fats which contain at least 3 % (from peak areas) of these polymers.
This method is applicable to frying fats, and fats and oils that have been thermally treated. It can also be applied to
the determination of polymers in fats for animal feedstuffs.
NOTE 1 Polymerized triglycerides (strictly speaking dimeric and oligomeric triglycerides) are formed during the heating of
fats and oils, thus the method serves to assess the thermal deterioration of frying fats with use.
NOTE 2 In the case of analysis of fats from animal feeding stuffs, the extraction method used can have an influence on the
result (see ISO 6492).
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent edition of the normative document indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 661:1989, Animal and vegetable fats and oils — Preparation of test sample
3 Term and definition
For the purposes of this International Standard, the following term and definition applies.
3.1
polymerized triglycerides
constituents of heated fats and oils that are determined by HPSEC under the conditions specified in this
International Standard
NOTE The content is expressed as a percentage of all peaks from eluted acylglycerides (TAGs, PTAGs, DAGs and
MAGs).
4Principle
The sample is dissolved in tetrahydrofuran then the polymerized triglycerides are separated by gel permeation
chromatography according to molecular size. Detection of the compounds is realized by means of a refractive
index detector.
© ISO 2001 – All rights reserved 1
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SIST EN ISO 16931:2002
ISO 16931:2001(E)
5 Reagents
Use only reagents of recognized analytical grade.
5.1 Tetrahydrofuran, possibly stabilized with BHT (0,1%), degassed.
It is important that the tetrahydrofuran used to dissolve the sampl
...
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