EN ISO 13885-3:2021
(Main)Gel permeation chromatography (GPC) - Part 3: Water as eluent (ISO 13885-3:2020)
Gel permeation chromatography (GPC) - Part 3: Water as eluent (ISO 13885-3:2020)
This document specifies the determination of the molar-mass distribution and the average molar mass values Mn (number average) and Mw (weight average) of polymers that are soluble in water by gel permeation chromatography (GPC).
NOTE Also known as size exclusion chromatography (SEC).
This method is applicable to neutral polymers and polyanions (e.g. polycarboxylates, polysaccharides, fully hydrolyzed polyvinyl alcohols and high-molecular polyethylene oxides). It is not applicable to polycations [e.g. polyvinylpyrrolidone, polyvinylpyridine, salts of poly(diallyl‑N,N‑dimethyl‑azacyclopentane), chitosan].
Despite good solubility in the mobile phase and even though the chromatograms obtained show good repeatability, it is possible that this method cannot be used with certain polymer types because of specific interactions (e.g. adsorption) within the sample/eluent/column system (see also Clause 12).
The conditions specified in this document are not applicable to the GPC analysis of polymer samples with Mw values greater than 106 g/mol and/or polymers with elution limits outside the calibration range (see 7.6 and Annex C).
This document includes no correction methods (e.g. for the elimination of peak broadening). If absolute molar mass values are required, an absolute method (e.g. membrane osmometry for Mn or light scattering for Mw) can be used.
Gelpermeationschromatographie (GPC) - Teil 3: Wasser als Elutionsmittel (ISO 13885-3:2020)
Dieses Dokument legt die Ermittlung der Molmassenverteilung und der Molmassenmittelwerte Mn (Zahlenmittel) und Mw (Gewichtsmittel) von wasserlöslichen Polymeren durch Gelpermeationschromatographie (GPC) fest.
ANMERKUNG Im Englischen auch als „size exclusion chromatography“ (SEC), übersetzt „Größenausschlusschromatographie“, bekannt.
Dieses Verfahren ist anwendbar auf neutrale Polymere und Polyanionen (z. B. Polycarboxylate, Polysaccharide, vollverseifte Polyvinylalkohole und hochmolekulare Polyethylenoxide). Es ist nicht anwendbar auf Polykationen [z. B. Polyvinylpyrrolidon, Polyvinylpyridin, Salze des Poly(diallyl-N,N-dimethyl-azacyclopentan), Chitosan].
Trotz guter Löslichkeit in der mobilen Phase und guter Wiederholbarkeit der resultierenden Chromatogramme ist es auf Grund spezifischer Wechselwirkungen (z. B. Adsorption) in dem System aus Probe/Elutionsmittel/Trennmaterial möglich, dass dieses Verfahren auf einzelne Polymere nicht angewandt werden kann (siehe auch Abschnitt 12).
Die in diesem Dokument festgelegten Bedingungen sind nicht anwendbar bei GPC-Analysen von Polymerproben mit Mw-Werten größer 106 g/mol und/oder von Polymeren, deren Elutionsgrenzen außerhalb des Kalibrierbereiches liegen (siehe 7.6 und Anhang C).
Dieses Dokument umfasst keine Korrekturverfahren (z. B. Elimination der Peakverbreiterung). Wenn absolute Molmassenwerte benötigt werden, kann auf ein Absolutverfahren (z. B. Membranosmometrie für Mn oder Lichtstreuung für Mw) zurückgegriffen werden.
WARNUNG - Dieses Dokument kann den Umgang mit gefährlichen Substanzen und Ausrüstungsteilen und die Ausführung gefährlicher Arbeitsgänge einschließen. Es erhebt nicht den Anspruch, auf alle gegebenenfalls vorhandenen und mit seiner Anwendung verbundenen Sicherheitsprobleme hinzuweisen. Es liegt in der Verantwortung des Anwenders, geeignete Vorkehrungen für den Arbeits‑ und Gesundheitsschutz zu treffen und die Übereinstimmung mit jeglichen nationalen Bestimmungen sicherzustellen.
Chromatographie par perméation de gel (GPC) - Partie 3: Eluant à l'eau (ISO 13885-3:2020)
Le présent document spécifie la détermination de la distribution de masses molaires et des valeurs de masse molaire moyenne Mn (moyenne en nombre) et Mw (moyenne en masse) de polymères solubles dans l’eau par chromatographie de perméation de gel (GPC, de l’anglais «gel permeation chromatography »).
NOTE Également appelée chromatographie d’exclusion stérique (SEC, de l’anglais «size exclusion chromatography »).
Cette méthode est applicable aux polymères neutres et aux polyanions [par exemple, polycarboxylates, polysaccharides, alcools polyvinyliques totalement hydrolysés et poly(oxyde d’éthylène) à masse moléculaire élevée]. Elle n’est pas applicable aux polycations [par exemple, polyvinylpyrrolidone, polyvinylpyridine, sels de poly(diallyl-N,N-diméthyl-azacyclopentane), chitosane].
Malgré une bonne solubilité dans la phase mobile et même si les chromatogrammes obtenus présentent une bonne répétabilité, il est possible que cette méthode ne puisse pas être utilisée avec certains types de polymère du fait d’interactions spécifiques (par exemple, adsorption) dans le système échantillon/éluant/colonne (voir également Article 12).
Les conditions spécifiées dans le présent document ne sont pas applicables à l’analyse GPC d’échantillons de polymères présentant des valeurs Mw supérieures à 106 g/mol et/ou de polymères dont les limites d’élution se situent en dehors de la gamme d’étalonnage (voir 7.6 et Annexe C).
Le présent document n’inclut pas de méthode de correction (par exemple, pour l’élimination d’un élargissement des pics). Si des valeurs absolues de masse molaire sont exigées, une méthode absolue (par exemple, osmométrie à membrane pour Mn ou diffusion de lumière pour Mw) peut être utilisée.
Gelska permeacijska kromatografija (GPC) - 3. del: Voda kot izpiralna tekočina (eluent) (ISO 13885-3:2020)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2021
Gelska permeacijska kromatografija (GPC) - 3. del: Voda kot izpiralna tekočina
(eluent) (ISO 13885-3:2020)
Gel permeation chromatography (GPC) - Part 3: Water as eluent (ISO 13885-3:2020)
Gelpermeationschromatographie (GPC) - Teil 3: Wasser als Elutionsmittel (ISO 13885-
3:2020)
Chromatographie par perméation de gel (GPC) - Partie 3: Eluant à l'eau (ISO 13885-
3:2020)
Ta slovenski standard je istoveten z: EN ISO 13885-3:2021
ICS:
87.060.20 Veziva Binders
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 13885-3
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2021
EUROPÄISCHE NORM
ICS 87.060.20
English Version
Gel permeation chromatography (GPC) - Part 3: Water as
eluent (ISO 13885-3:2020)
Chromatographie par perméation de gel (GPC) - Partie Gelpermeationschromatographie (GPC) - Teil 3:
3: Eluant à l'eau (ISO 13885-3:2020) Wasser als Elutionsmittel (ISO 13885-3:2020)
This European Standard was approved by CEN on 23 August 2021.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
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© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13885-3:2021 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 13885-3:2020 has been prepared by Technical Committee ISO/TC 35 "Paints and
varnishes” of the International Organization for Standardization (ISO) and has been taken over as
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2022, and conflicting national standards shall
be withdrawn at the latest by March 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 13885-3:2020 has been approved by CEN as EN ISO 13885-3:2021 without any
modification.
INTERNATIONAL ISO
STANDARD 13885-3
First edition
2020-07
Gel permeation chromatography
(GPC) —
Part 3:
Water as eluent
Chromatographie par perméation de gel (GPC) —
Partie 3: Eluant à l'eau
Reference number
ISO 13885-3:2020(E)
©
ISO 2020
ISO 13885-3:2020(E)
© ISO 2020
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ISO 13885-3:2020(E)
Contents Page
Foreword .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Apparatus . 2
5.1 Eluent supply . 3
5.2 Pump . 3
5.3 Injection system . 3
5.4 Separation columns . 3
5.5 Column temperature control . 5
5.6 Detector . 6
6 Reagents . 6
7 Calibration of the apparatus . 6
7.1 General . 6
7.2 Specification for the calibration standard . 6
7.3 Preparation of the calibration solutions for injection . 7
7.4 Conditions for calibration runs . 7
7.5 Measurement of elution volume . 7
7.6 Plotting the calibration curve . 7
8 Sampling . 8
9 Preparation for the test . 8
9.1 Preparation of the injection solution . 8
9.2 Preparation of the apparatus . 9
10 Analytical parameters . 9
11 Data acquisition and evaluation . 9
11.1 General . 9
11.2 Calculation of the net chromatogram from the raw data .10
11.2.1 Determination of the baseline .10
11.2.2 Correction of the measured values and of the net chromatogram .10
11.2.3 Evaluation limits .10
11.3 Calculation of the average values .10
11.4 Calculation of the distribution curves .11
12 Precision .12
12.1 General .12
12.2 Repeatability .12
12.3 Reproducibility .12
13 Test report .13
13.1 General .13
13.2 General data on the equipment and settings .13
13.2.1 Data on the equipment used .13
13.2.2 Calibration .13
13.2.3 Evaluation .14
13.3 Special data on the sample .14
Annex A (informative) Conversion of experimental parameters for variant column sizes .15
Annex B (informative) Example of a data sheet for a polymer standard .16
Annex C (informative) Explanations .
...
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