EN 14122:2014

Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography

This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.
For further information on the validation, see Clause 8 and Annex B.

Lebensmittel - Bestimmung von Vitamin B1 mit Hochleistungs-Flüssigchromatographie

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin B1 in Lebensmitteln mit Hochleistungsflüssigchromatographie (HPLC) mit Enzymbehandlung und Vor- oder Nachsäulenderivati¬sierung fest. Diese Bestimmung wurde in zwei Ringversuchen validiert. In dem einen Ringversuch wurde Vollkornmehl, Milchpulver/sprühgetrocknete Milch, gefriergetrocknetes Mischgemüse und gefrier¬getrocknete Schweineleber mit Gehalten von 0,295 mg/100 g bis 0,807 mg/100 g analysiert. Im zweiten Ringversuch wurde Krankenhausnahrung, Babynahrung mit Gemüse, Milchpulver, Fertignahrungsmittel mit Früchten, Hefe, Getreide, Schokoladenpulver und Nahrungsergänzungsmittel von 0,11 mg/100 g bis 468 mg/100 g untersucht. Vitamin B1 ist der Massenanteil an Gesamtthiamin einschließlich der phosphorylierten Derivate.

Produits alimentaires - Détermination de la teneur en vitamine B1 par chromatographie liquide haute performance

La présente Norme européenne spécifie une méthode de dosage de la vitamine B1 présente dans les produits alimentaires par chromatographie liquide haute performance (CLHP) avec traitement enzymatique et dérivation pré- ou post-colonne. Cette méthode a été validée lors de deux essais interlaboratoires. Le premier essai concernait l’analyse d’échantillons de farine complète, de lait en poudre/lait séché par atomisation, d’assortiments de légumes lyophilisés et de foie de porc lyophilisé allant de 0,295 mg/100 g à 0,807 mg/100 g. Le deuxième essai s’appliquait à l’analyse d’échantillons de solution pour alimentation entérale, d’aliment pour bébé aux légumes, de lait en poudre, de substitut de repas aux fruits, de levure, de céréale, de chocolat en poudre et de complément alimentaire allant de 0,11 mg/100 g à 486 mg/100 g. La vitamine B1 correspond à la fraction massique de thiamine totale, y compris ses dérivés phosphorylés.
Pour plus d'informations sur la validation, voir l'Article 8 et l'Annexe B.

Živila - Določevanje vitamina B1 s tekočinsko kromatografijo visoke ločljivosti

Standard EN 14122 navaja metodo za določanje vitamina B1 v živilih s tekočinsko kromatografijo visoke ločljivosti (HPLC) z encimsko obdelavo ter pred- ali post-kolonsko derivatizacijo. Ta metoda je bila validirana z dvema medlaboratorijskima študijama. Prva študija je bila namenjena analizi vzorcev polnozrnate moke, mleka v prahu/atomiziranega mleka, zmrznjene mešane zelenjave in zmrznjenih prašičjih jeter, ki vsebujejo od 0,295 mg/100 g do 0,807 mg/100 g vitamina B1. Druga študija je bila namenjena analizi vzorcev raztopin za krmljenje, zelenjavnih formul za dojenčke, mleka v prahu, grobe moke s sadjem, kvasa, žitaric, čokolade v prahu in prehranskih nadomestil, ki vsebujejo od 0,11 mg/100 g do 486 mg/100 g vitamina B1. Vitamin B1 je masni delež celotne vrednosti tiamina, vključno z njegovimi fosforiliranimi derivati. Za več informacij glede validacije glejte točko 8 in dodatek B.

General Information

• Status: Published
• Publication Date: 03-Jun-2014
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 9 - Vitamins and Carotenoids
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 28-Oct-2020
• Ref Project: SIST EN 14122:2014 - Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography

Relations

• Replaces: EN 14122:2003 - Foodstuffs - Determination of vitamin B1 by HPLC
• Effective Date: 11-Jun-2014
• Replaces: EN 14122:2003/AC:2005 - Foodstuffs - Determination of vitamin B1 by HPLC
• Effective Date: 11-Jun-2014

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin B1 mit Hochleistungs-FlüssigchromatographieProduits alimentaires - Dosage de la vitamine B1 par chromatographie liquide haute performanceFoodstuffs - Determination of vitamin B1 by high performance liquid chromatography67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 14122:2014SIST EN 14122:2014en,fr,de01-september-2014SIST EN 14122:2014SLOVENSKI
STANDARDSIST EN 14122:2003/AC:2006SIST EN 14122:20031DGRPHãþD



SIST EN 14122:2014



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 14122
June 2014 ICS 67.050 Supersedes EN 14122:2003English Version
Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography
Produits alimentaires - Détermination de la teneur en vitamine B1 par chromatographie liquide haute performance Lebensmittel - Bestimmung von Vitamin B1 mit Hochleistungs-Flüssigchromatographie This European Standard was approved by CEN on 17 April 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 14122:2014 ESIST EN 14122:2014



EN 14122:2014 (E) 2 Contents Page Foreword . 3 1 Scope . 4 2 Normative references . 4 3 Principle . 4 4 Reagents . 4 5 Apparatus . 7 6 Procedure . 8 7 Calculation . 10 8 Precision . 11 9 Test report . 12 Annex A (informative)
Examples of HPLC chromatograms . 13 Annex B (informative)
Precision data . 15 Annex C (informative)
Alternative HPLC systems . 18 Annex D (informative)
Vitamin B1 compound: 2-(1-hydroxyethyl)thiamin (HET) performing post-column derivatization . 19 Bibliography .
...