EN 14122:2014
(Main)Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography
Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography
This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.
For further information on the validation, see Clause 8 and Annex B.
Lebensmittel - Bestimmung von Vitamin B1 mit Hochleistungs-Flüssigchromatographie
Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin B1 in Lebensmitteln mit Hochleistungsflüssigchromatographie (HPLC) mit Enzymbehandlung und Vor- oder Nachsäulenderivati¬sierung fest. Diese Bestimmung wurde in zwei Ringversuchen validiert. In dem einen Ringversuch wurde Vollkornmehl, Milchpulver/sprühgetrocknete Milch, gefriergetrocknetes Mischgemüse und gefrier¬getrocknete Schweineleber mit Gehalten von 0,295 mg/100 g bis 0,807 mg/100 g analysiert. Im zweiten Ringversuch wurde Krankenhausnahrung, Babynahrung mit Gemüse, Milchpulver, Fertignahrungsmittel mit Früchten, Hefe, Getreide, Schokoladenpulver und Nahrungsergänzungsmittel von 0,11 mg/100 g bis 468 mg/100 g untersucht. Vitamin B1 ist der Massenanteil an Gesamtthiamin einschließlich der phosphorylierten Derivate.
Produits alimentaires - Détermination de la teneur en vitamine B1 par chromatographie liquide haute performance
La présente Norme européenne spécifie une méthode de dosage de la vitamine B1 présente dans les produits alimentaires par chromatographie liquide haute performance (CLHP) avec traitement enzymatique et dérivation pré- ou post-colonne. Cette méthode a été validée lors de deux essais interlaboratoires. Le premier essai concernait l’analyse d’échantillons de farine complète, de lait en poudre/lait séché par atomisation, d’assortiments de légumes lyophilisés et de foie de porc lyophilisé allant de 0,295 mg/100 g à 0,807 mg/100 g. Le deuxième essai s’appliquait à l’analyse d’échantillons de solution pour alimentation entérale, d’aliment pour bébé aux légumes, de lait en poudre, de substitut de repas aux fruits, de levure, de céréale, de chocolat en poudre et de complément alimentaire allant de 0,11 mg/100 g à 486 mg/100 g. La vitamine B1 correspond à la fraction massique de thiamine totale, y compris ses dérivés phosphorylés.
Pour plus d'informations sur la validation, voir l'Article 8 et l'Annexe B.
Živila - Določevanje vitamina B1 s tekočinsko kromatografijo visoke ločljivosti
Standard EN 14122 navaja metodo za določanje vitamina B1 v živilih s tekočinsko kromatografijo visoke ločljivosti (HPLC) z encimsko obdelavo ter pred- ali post-kolonsko derivatizacijo. Ta metoda je bila validirana z dvema medlaboratorijskima študijama. Prva študija je bila namenjena analizi vzorcev polnozrnate moke, mleka v prahu/atomiziranega mleka, zmrznjene mešane zelenjave in zmrznjenih prašičjih jeter, ki vsebujejo od 0,295 mg/100 g do 0,807 mg/100 g vitamina B1. Druga študija je bila namenjena analizi vzorcev raztopin za krmljenje, zelenjavnih formul za dojenčke, mleka v prahu, grobe moke s sadjem, kvasa, žitaric, čokolade v prahu in prehranskih nadomestil, ki vsebujejo od 0,11 mg/100 g do 486 mg/100 g vitamina B1. Vitamin B1 je masni delež celotne vrednosti tiamina, vključno z njegovimi fosforiliranimi derivati. Za več informacij glede validacije glejte točko 8 in dodatek B.
General Information
Relations
Overview
EN 14122:2014 (CEN) specifies a validated laboratory method for the determination of vitamin B1 (thiamin) in foodstuffs using high performance liquid chromatography (HPLC). The method measures the mass fraction of total thiamin, including phosphorylated derivatives, by combining acid hydrolysis, enzymatic dephosphorylation and pre‑ or post‑column derivatization to the fluorescent thiochrome derivative. The procedure has been validated in two interlaboratory studies across a wide concentration range (about 0.11–486 mg/100 g in various food matrices).
Key Topics and Requirements
- Scope and validation: Method validated on diverse matrices (e.g., milk powders, wholemeal flour, baby foods, tube feeding solutions, yeast, cereals, liver, supplements) with published precision data and annexed interlaboratory results.
- Analytical principle: Acid hydrolysis (pH ≤ 2) to liberate thiamin, enzymatic dephosphorylation to convert phosphorylated thiamin derivatives to free thiamin, oxidation/derivatization to thiochrome, and HPLC separation with fluorescence detection.
- Reagents and standards: Specifies reagent grades (e.g., methanol HPLC grade), buffer systems, potassium hexacyanoferrate derivatization reagents, and certified reference standard substances for thiamin and phosphorylated thiamin forms.
- Instrumentation: Requires an HPLC system with fluorescence detector and suitable columns; options for pre‑column or post‑column oxidation/derivatization and guidance on column selection and mobile phases are provided.
- Procedure controls: Includes sample homogenization, extraction (autoclave or heated hydrolysis), enzyme treatment optimization (pH, temperature, incubation), filtration, calibration with external standards and blank/quality checks to verify dephosphorylation efficiency.
- Documentation: Test report content, calculation method and precision tables are specified; Annexes include example chromatograms, precision data and alternative HPLC setups.
Applications and Users
EN 14122:2014 is intended for:
- Food testing laboratories performing routine or compliance analysis for nutritional labeling and quality control.
- Manufacturers of dairy, baby food, cereals, tube-feeding formulas and supplements for product specification and QA.
- Regulatory bodies and accreditation laboratories that require a standardized, validated method for enforcement or surveillance.
- Research groups studying thiamin content and bioavailability in complex food matrices.
Practical value: the standard delivers a reproducible, interlaboratory‑validated HPLC protocol for accurate quantification of vitamin B1 across a broad range of food types and concentrations.
Related Standards
- EN ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
- The standard supersedes EN 14122:2003; consult the Annexes in EN 14122:2014 for precision data, chromatograms and alternative HPLC system guidance.
Frequently Asked Questions
EN 14122:2014 is a standard published by the European Committee for Standardization (CEN). Its full title is "Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography". This standard covers: This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives. For further information on the validation, see Clause 8 and Annex B.
This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives. For further information on the validation, see Clause 8 and Annex B.
EN 14122:2014 is classified under the following ICS (International Classification for Standards) categories: 67.050 - General methods of tests and analysis for food products. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 14122:2014 has the following relationships with other standards: It is inter standard links to EN 14122:2003, EN 14122:2003/AC:2005. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin B1 mit Hochleistungs-FlüssigchromatographieProduits alimentaires - Dosage de la vitamine B1 par chromatographie liquide haute performanceFoodstuffs - Determination of vitamin B1 by high performance liquid chromatography67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 14122:2014SIST EN 14122:2014en,fr,de01-september-2014SIST EN 14122:2014SLOVENSKI
STANDARDSIST EN 14122:2003/AC:2006SIST EN 14122:20031DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 14122
June 2014 ICS 67.050 Supersedes EN 14122:2003English Version
Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography
Produits alimentaires - Détermination de la teneur en vitamine B1 par chromatographie liquide haute performance Lebensmittel - Bestimmung von Vitamin B1 mit Hochleistungs-Flüssigchromatographie This European Standard was approved by CEN on 17 April 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 14122:2014 ESIST EN 14122:2014
Examples of HPLC chromatograms . 13 Annex B (informative)
Precision data . 15 Annex C (informative)
Alternative HPLC systems . 18 Annex D (informative)
Vitamin B1 compound: 2-(1-hydroxyethyl)thiamin (HET) performing post-column derivatization . 19 Bibliography . 21
1) The information of the suppliers of Taka-Diastase, Pfaltz & Bauer, Waterbury, CT 06708, USA (No T00040), and Serva is given for the convenience of users of this European standard and does not constitute an endorsement by CEN of the product named. Equivalent products may be used if they can be shown to lead to the same results. SIST EN 14122:2014
HCl) ≈ 0,1 mg/ml. Dissolve an accurately weighed amount of the thiamin chloride hydrochloride standard substance (4.23.1) in a defined volume of an appropriate solvent, for example 10 mg of vitamin B1 standard substance in 100 ml of hydrochloric acid solution (4.6). This solution can be stored for four weeks at + 4 °C. 4.24.2 Thiamin monophosphate stock solution, (C12H17ClN4O4PS) ≈ 0,1 mg/ml. Dissolve an accurately weighed amount of the thiamin monophosphate chloride (4.23.2) in a defined volume of an appropriate solvent, for example 10 mg of thiamin monophosphate chloride in 100 ml of hydrochloric acid solution (4.6). This solution can be stored for four weeks at - 20 °C. 4.24.3 Thiamin pyrophosphate stock solution, (C12H19ClN4O7P2S) ≈ 0,1 mg/ml. Dissolve an accurately weighed amount of the thiamin pyrophosphate chloride (4.23.3) in a defined volume of an appropriate solvent, for example 10 mg of the thiamin pyrophosphate chloride in 100 ml of hydrochloric acid solution (4.6). 4.24.4 Concentration tests - thiamin chloride hydrochloride Dilute 10 ml of the thiamin chloride hydrochloride solution (4.24.1) with hydrochloric acid solution (4.6) in a 100 ml volumetric flask to the mark. Measure the absorbance of this solution at the maximum of about 247 nm (A247) in a 1 cm cell against hydrochloric solution (4.6) in the reference cell using an UV spectrometer (5.1). Calculate the mass concentration, , in microgram per millilitre thiamin chloride hydrochloride solution (4.24.1) using Formula (1): ερ0001247⋅⋅=MA (1) where 0 is the molar absorption coefficient of thiamin chloride hydrochloride at the maximum wavelength of about 247 nm. The value is 14 200 l · mol−1 · cm−1. This value is calculated from the extinction coefficient, 1%1cmE = 421, in 0,1 mol/l HCl [8][7] and the molar mass, M = 337,21. The value is given with four significant figures; M is the molar mass, in grams per mol. The value is 337,21; A247 is the absorption value of the thiamin chloride hydrochloride solution. 4.25 Standard solutions 4.25.1 Thiamin chloride hydrochloride standard solution, (C12H17ClN4lS
HCl) ≈ 1
Pipette 1 ml to 10 ml of the thiamin chloride hydrochloride solution (4.24.1) into a 100 ml volumetric flask and dilute to the mark with the appropriate solvent, e.g. hydrochloric acid solution (4.6). This solution can be stored at 4 °C in the dark for 1 month. 4.25.2 Thiamin monophosphate standard solution, (C12H17ClN4O4mS) ≈ 1
Pipette 1 ml to 10 ml of the thiamin monophosphate solution (4.24.2) into a 100 ml volumetric flask and dilute to the mark with the appropriate solvent, e.g. hydrochloric acid solution (4.6). This solution can be stored at 4 °C in the dark for 1 month. SIST EN 14122:2014
Pipette 1 ml to 10 ml of the thiamin pyrophosphate solution (4.24.3) into a 100 ml volumetric flask and dilute to the mark with the appropriate solvent, e.g. hydrochloric acid solution (4.6). This solution can be stored at 4 °C in the dark for 1 month. 5 Apparatus Usual laboratory apparatus, glassware, and the following: 5.1 UV spectrometer, UV spectrometer, capable of measuring absorption at defined wavelengths (247 nm), with appropriate cells, e.g. of 1 cm length. 5.2 Autoclave or heating device, autoclave for extraction purpose, e.g. pressure cooker type, with pressure or temperature reading device, electrical heating device or water bath. 5.3 HPLC system HPLC system, consisting of a pump, a sample injecting device, a fluorescence detector with an excitation and emission wavelength set at e.g. 366 nm and 435 nm, respectively (see Annex C), and an evaluation system such as an integrator. 5.4 HPLC column 5.4.1 General Other particle sizes or column dimensions than those specified in this European Standard may be used. Separation parameters shall be adapted to such materials to guarantee equivalent results. The performance criterion for suitable analytical columns is the baseline resolution of the thiamin from interferences2). 5.4.2 Pre-column oxidation Analytical columns, e.g. Lichrospher® 60 RP Select B 2), particle size of 5 µm, diameter 4,0 mm to 4,6 mm, length 100 mm to 250 mm. 5.4.3 Post-column oxidation Analytical columns, e.g. Supelco® LC-18- DB 2), particle size of 5 µm, diameter 4,0 mm to 4,6 mm, length 100 mm to 250 mm. 5.5 Filter device Filtering of the mobile phase as well as of the sample solution through a membrane filter with, e.g. a pore size of 0,45 µm, prior to use or injection will increase longevity of the columns. 5.6 Post-column reactor pump and derivatization tube, a suitable reagent delivery system, a T-type connecting tube and a derivatization tube (e.g. 10 m x 0,33 mm).
2) Suitable silica column packing materials available commercially are Lichrosorb® Si 60, Spherisorb® Si, Hypersil® Si and Lichrospher® 100 DIOL. Suitable RP column packing materials are Spherisorb® ODS, µ-Bondapak radial C18, Supelco. LC-18- DB and Hypersil® ODS. This information is given for the convenience of users of this European Standard and does not constitute an endorsement by CEN of these products. SIST EN 14122:2014
Preparation of the test sample Homogenize the test sample. Grind coarse material with an appropriate mill and mix again. Measures such as pre-cooling shall be taken to avoid exposing to high temperature for long periods of time. 6.2 Preparation of the sample test solution 6.2.1 Extraction Weigh an appropriate amount of the test sample to the nearest mg, e.g. 2 g to 10 g in a conical flask. Add a defined volume ranging from 60 ml to 200 ml of hydrochloric acid solution (4.6), or sulfuric acid solution (4.7). The pH of the solution should not be higher than pH = 2,0. Cover the container with a watch glass and either autoclave the test portion at 121 °C for 30 min, or heat it at 100 °C for 60 min. The data from the BCR study have shown that a wide range of conditions for the acid hydrolysis can be applied (temperature 95 °C to 130 °C, time 15 min to 60 min). The higher the temperature is, the shorter the time should be. 6.2.2 Enzyme treatment After cooling to room temperature adjust the extract to the optimal pH for the enzyme used with sodium acetate solution (4.16) or (4.17) and add a suitable amount of enzyme or enzyme mixture (4.15) to the sample. Incubate the mixture at the optimal time and temperature for the enzyme(s) used. After cooling to room temperature transfer the solution to a volumetric flask using distilled water, or another appropriate solvent and dilute the sample test solution to a defined volume (Vts). For each enzyme used, optimal pH, incubation time and incubation temperature shall be checked. To ensure an optimal dephosphorylation, the enzymatic step shall be checked with analysis of samples spiked with thiamin monophosphate chloride (4.23.2) or thiamin pyrophosphate chloride (4.23.3), and a material similar in sample type as the test sample. This material should be a certified reference material. The amount of thiamin possibly brought in with the enzyme(s) (4.15) shall be considered in the calculation of the result. NOTE For determination of the precision data given in this European Standard in Table B.1, Table B.2 and Table B.3, Taka-Diastase and Taka-Diastase combined with -amylase from barley was used for dephosphorylation under the following conditions. The extract was adjusted to pH = 4,0 and pH = 4,5, respectively, with sodium acetate solution (4.16) or (4.17) and 100 mg of Taka-Diastase and 10 -amylase per gram of sample was added. The mixture was incubated at 37 °C to 45 °C for 4 h to 24 h, see [5], [10] and [16]. 6.2.3 Sample test solution If necessary, filter the sample solution (6.2.2) through a filter paper or a 0,45 µm membrane filter. Centrifugation may also be used. This is the sample test solution for oxidation (6.3.2 or 6.3.3). 6.3 Oxidation of thiamin to thiochrome 6.3.1 General The oxidation may be performed either pre-column (6.3.2) or post-column (6.3.3). SIST EN 14122:2014
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Die Norm EN 14122:2014 stellt einen bedeutenden Fortschritt in der Bestimmung von Vitamin B1 in Lebensmitteln dar, indem sie eine präzise Methode mittels Hochleistungsflüssigkeitschromatographie (HPLC) festlegt. Der Anwendungsbereich dieser Norm ist klar definiert und umfasst die Analyse von verschiedenen Lebensmitteln wie Vollkornmehl, Milchpulver, gefriergetrockneten Gemüsemischungen und Leber von Schweinen sowie von Nahrungsaufnahme-Lösungen, Babynahrung, Pulvermilch und Nahrungsmittelergänzungen. Ein herausragendes Merkmal dieser Norm ist die Validierung, die in zwei Interlaborstudien durchgeführt wurde und somit die Zuverlässigkeit und Reproduzierbarkeit der Methode sichert. Besonders hervorzuheben ist, dass die untersuchten Proben eine breite Spanne der Vitamin B1 Konzentrationen abdecken, was die Vielseitigkeit und Anwendbarkeit der Methode unterstreicht. Dies gewährleistet, dass sowohl niedrigere als auch höhere Gehalte an Vitamin B1 in verschiedenen Lebensmitteln effektiv gemessen werden können. Die Norm geht zudem auf die Bedeutung der enzymatischen Behandlung sowie der Vor- oder Nachsäulenderivatisierung ein, was für die Genauigkeit der Analyse von essentieller Bedeutung ist. Die klare Struktur der Norm, einschließlich der detaillierten Informationen zur Validierung in Abschnitt 8 und Anhang B, ermöglicht es Fachleuten in der Lebensmittelindustrie, die Methodik optimal anzuwenden und die Qualität der Produkte sicherzustellen. Insgesamt bietet die EN 14122:2014 eine fundierte Grundlage für die präzise Bestimmung von Vitamin B1 in Lebensmitteln und trägt damit entscheidend zur Lebensmittelsicherheit und zur Förderung der Gesundheit der Verbraucher bei.
EN 14122:2014の標準は、食品中のビタミンB1の定量分析に関して非常に重要な手法を提供しています。この文書は、高性能液体クロマトグラフィー（HPLC）を利用したビタミンB1の測定方法を明確に定義しており、それに加えて酵素処理や前処理および後処理における誘導体化を含むなど、最新の技術を取り入れています。 この標準の強みは、実施された二つのインターロボラトリー研究によってその方法が検証されている点です。最初の研究では、全粒粉、ミルクパウダー、フリーズドライの混合野菜、豚の肝臓サンプルなど、0.295 mg/100 gから0.807 mg/100 gの範囲において測定が確認されています。次に、チューブフィーディング用の溶液、野菜入りのベビーフード、粉乳、フルーツ入りの食事、酵母、シリアル、ココアパウダー、食品サプリメントにおいても、0.11 mg/100 gから486 mg/100 gまでの幅広いビタミンB1の量が正確に測定されています。 このように、EN 14122:2014は食品中のビタミンB1の質と安全性を評価する上で非常に有用な標準であり、食品業界における従業員や消費者保護に寄与するものです。また、ビタミンB1の全チアミンおよびそのリン酸化誘導体の質量分率を考慮に入れているため、より包括的な分析が可能となっています。 この標準に関するさらなる情報、特に検証に関しては第8条および付録Bを参照することが推奨されます。このように、EN 14122:2014は食品中のビタミンB1分析において信頼性の高い基準を提供しており、業界のニーズに応えるものとなっています。
La norme SIST EN 14122:2014 offre une méthode validée pour la détermination de la vitamine B1 dans les denrées alimentaires par chromatographie liquide haute performance (HPLC). Son champ d'application est particulièrement large, englobant une variété d'échantillons tels que la farine complète, le lait en poudre, les légumes déshydratés et différents types d'aliments pour bébés, ce qui témoigne de sa pertinence dans plusieurs secteurs de l'agroalimentaire. L'analyse a été corroborée par deux études interlaboratoires qui apportent une solidité à la méthode. La première étude a porté sur des échantillons variés de farine complète et de foie de porc, garantissant que la méthode est efficace pour des niveaux de vitamine B1 allant de 0,295 mg/100 g à 0,807 mg/100 g. La seconde étude a élargi le spectre d'analyse à des solutions alimentaires plus spécifiques, avec des concentrations allant de 0,11 mg/100 g à 486 mg/100 g. Cela démontre non seulement la sensibilité de la méthode, mais aussi sa capacité à être appliquée à une large gamme d'aliments, offrant ainsi des résultats fiables et précis. L'utilisation de traitements enzymatiques ainsi que de la dérivatisation pre- ou post-colonne dans le cadre de la procédure de HPLC assure une analyse approfondie et détaillée de la vitamine B1, incluant ses dérivés phosphorylés. Ces aspects renforcent l'importance de la norme dans l'analyse nutritionnelle et le contrôle de la qualité des aliments. En conclusion, la norme SIST EN 14122:2014 se présente comme une référence incontournable pour les laboratoires et les entreprises du secteur alimentaire souhaitant quantifier avec exactitude la vitamine B1 dans divers produits, garantissant ainsi des normes de qualité élevées. Pour plus d'informations sur la validation, il est conseillé de consulter la Clause 8 ainsi que l'Annexe B.
The EN 14122:2014 standard provides a comprehensive method for the determination of vitamin B1 in food products through high performance liquid chromatography (HPLC), coupled with enzymatic treatment and derivatization techniques. Its scope covers a wide range of foodstuffs, emphasizing its versatility for different matrices, including whole meal flour, milk powder, freeze-dried vegetables, and various baby foods. One of the significant strengths of this standard is its robust validation through two interlaboratory studies, which enhances the credibility and reliability of the method. The first study focused on a diverse array of samples, demonstrating the method's effectiveness at quantifying vitamin B1 concentrations ranging from 0.295 mg/100 g to 0.807 mg/100 g. The second study further illustrates the method's applicability across various food categories, confirming its capacity to accurately measure vitamin B1 in products with concentrations as low as 0.11 mg/100 g up to 486 mg/100 g. The clarity in the specifications regarding the mass fraction of total thiamin, including phosphorylated derivatives, adds to the standard's relevance in accurately determining vitamin B1 content. This is particularly vital for food safety and regulatory compliance, ensuring producers meet labeling requirements and consumers receive proper nutritional information. In summary, the EN 14122:2014 standard stands out due to its thorough methodological validation, comprehensive scope, and practical relevance in the food industry, making it a key reference for laboratories engaged in the analysis of vitamin B1 in foodstuffs.
SIST EN 14122:2014 표준은 식품 내 비타민 B1의 정량 분석을 위한 고성능 액체 크로마토그래피(HPLC) 방법을 규명하고 있습니다. 이 표준의 범위는 효소 처리와 전 또는 후 컬럼 유도체화를 포함한 HPLC 방법을 통해 비타민 B1을 측정하는 데 초점을 맞추고 있습니다. 이 표준의 강점 중 하나는 두 가지의 상호시험 연구에서 검증되었다는 점입니다. 첫 번째 연구에서는 전체 밀가루, 분유/스프레이 건조 우유, 냉동혼합 채소, 냉동돼지 간 샘플의 비타민 B1 농도가 0.295 mg/100 g에서 0.807 mg/100 g 사이에서 분석되었습니다. 두 번째 연구에서는 튜브 수유 용액, 야채가 포함된 아기 음식, 분유, 과일이 포함된 식사, 효모, 곡물, 초콜릿 가루 및 식품 보충제 샘플의 비타민 B1 농도가 0.11 mg/100 g에서 486 mg/100 g 사이에서 측정되었습니다. 비타민 B1은 총 티아민의 질량 비율로, 그 포스포릴화된 유도체도 포함된다는 점에서 특히 중요합니다. 이러한 요소들은 식품의 영양학적 평가와 안전성에 직접적인 영향을 미치므로, 이 표준은 식품 산업에서 필수적인 역할을 합니다. 참고로, 검증에 대한 더 자세한 정보는 조항 8 및 부록 B에서 확인할 수 있습니다. 따라서 SIST EN 14122:2014 표준은 비타민 B1의 정확한 분석을 위한 신뢰할 수 있는 기준을 제공하며, 식품 안전성과 품질 관리 분야에서 그 중요성이 더욱 부각됩니다.
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