EN ISO 10272-2:2017/A1:2023

Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique - Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and changes in the performance testing of culture media (ISO 10272-2:2017/Amd 1:2023)

Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zählung von Campylobacter spp. - Teil 2: Koloniezählverfahren - Änderung 1: Aufnahme von Verfahren zur molekularen Bestätigung und Identifizierung von thermotoleranten Campylobacter spp. und Änderungen bei der Leistungsprüfung von Nährmedien (ISO 10272-2:2017/Amd 1:2023)

No scope available

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le dénombrement de Campylobacter spp. - Partie 2: Technique par comptage des colonies - Amendment 1: Ajout de méthodes pour la confirmation et l’identification moléculaires de Campylobacter spp. thermotolérants, et modification des essais de performance des milieux de culture (ISO 10272-2:2017/Amd 1:2023)

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Campylobacter spp. - 2. del: Tehnika štetja kolonij - Dopolnilo A1: Vključitev metod za molekularno potrditev in identifikacijo toplotno stabilnih bakterij Campylobacter spp. ter spremembe pri preskušanju učinkovitosti gojišč (ISO 10272-2:2017/Amd 1:2023)

General Information

• Status: Published
• Publication Date: 07-Feb-2023
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 3 - Campylobacter
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 08-Feb-2023
• Due Date: 25-Jun-2021
• Completion Date: 08-Feb-2023
• Ref Project: SIST EN ISO 10272-2:2017/A1:2023 - Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique - Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and changes in the performance testing of culture media (ISO 10272-2:2017/Amd 1:2023)

Relations

• Amends: EN ISO 10272-2:2017 - Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique (ISO 10272-2:2017)
• Effective Date: 30-Jan-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2023
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti in števila Campylobacter spp. - 2. del: Tehnika štetja kolonij - Dopolnilo
A1: Vključitev metod za molekularno potrditev in identifikacijo toplotno stabilnih
bakterij Campylobacter spp. ter spremembe pri preskušanju učinkovitosti gojišč
(ISO 10272-2:2017/Amd 1:2023)
Microbiology of the food chain - Horizontal method for detection and enumeration of
Campylobacter spp. - Part 2: Colony-count technique - Amendment 1: Inclusion of
methods for molecular confirmation and identification of thermotolerant Campylobacter
spp. and changes in the performance testing of culture media (ISO 10272-2:2017/Amd
1:2023)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur
Zählung von Campylobacter spp. - Teil 2: Koloniezählverfahren - Änderung 1 (ISO 10272
-2:2017/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le
dénombrement de Campylobacter spp. - Partie 2: Technique par comptage des colonies
- Amendment 1: Ajout de méthodes pour la confirmation et l’identification moléculaires
de Campylobacter spp. thermotolérants, et modification des essais de performance des
milieux de culture (ISO 10272-2:2017/Amd 1:2023)
Ta slovenski standard je istoveten z: EN ISO 10272-2:2017/A1:2023
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10272-2:2017/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2023
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for
detection and enumeration of Campylobacter spp. - Part 2:
Colony-count technique - Amendment 1: Inclusion of
methods for molecular confirmation and identification of
thermotolerant Campylobacter spp. and correction of the
performance testing of the media (ISO 10272-2:2017/Amd
1:2023)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche et le dénombrement de Verfahren zum Nachweis und zur Zählung von
Campylobacter spp. - Partie 2: Technique par comptage Campylobacter spp. - Teil 2: Koloniezählverfahren -
des colonies - Amendment 1: Ajout de méthodes pour Änderung 1 (ISO 10272-2:2017/FDAM 1:2022)
la confirmation et l'identification moléculaires de
Campylobacter spp. thermotolérants, et modification
des essais de performance des milieux de culture (ISO
10272-2:2017/FDAM 1:2022)
This amendment A1 modifies the European Standard EN ISO 10272-2:2017; it was approved by CEN on 29 November 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10272-2:2017/A1:2023 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 10272-2:2017/A1:2023) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2023, and conflicting national standards shall
be withdrawn at the latest by August 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 10272-2:2017/Amd 1:2023 has been approved by CEN as EN ISO 10272-
2:2017/A1:2023 without any modification.

INTERNATIONAL ISO
STANDARD 10272-2
First edition
2017-06
AMENDMENT 1
2023-01
Microbiology of the food chain —
Horizontal method for detection and
enumeration of Campylobacter spp. —
Part 2:
Colony-count technique
AMENDMENT 1: Inclusion of methods
for molecular confirmation and
identification of thermotolerant
Campylobacter spp. and changes in the
performance testing of culture media
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Campylobacter spp. —
Partie 2: Technique par comptage des colonies
AMENDEMENT 1: Ajout de méthodes pour la confirmation et
l’identification moléculaires de Campylobacter spp. thermotolérants,
et modification des essais de performance des milieux de culture
Reference number
ISO 10272-2:2017/Amd.1:2023(E)
ISO 10272-2:2017/Amd.1:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 10272-2:2017/Amd.1:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
A list of all the parts in the ISO 10272 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iii
ISO 10272-2:2017/Amd.1:2023(E)
Microbiology of the food chain — Horizontal method for
detection and enumeration of Campylobacter spp. —
Part 2:
Colony-count technique
AMENDMENT 1: Inclusion of methods for molecular
confirmation and identification of thermotolerant
Campylobacter spp. and changes in the performance testing of
culture media
3.1
Replace the text with the following:
3.1
Campylobacter
genus of microorganisms of the family Campylobacteraceae, forming characteristic colonies on solid
selective media, such as modified Charcoal Cefoperazone Deoxycholate (mCCD) agar, when incubated
in a microaerobic atmosphere at 41,5 °C and displaying certain characteristics with biochemical
confirmation tests and by microscopy
Note 1 to entry: Microscopy, the biochemical confirmation tests and the characteristics of Campylobacter are
described in 9.4.
Note 2 to entry: This document targets the thermotolerant Campylobacter species relevant to human health.
The most frequently encountered and relevant to human health are Campylobacter jejuni and Campylobacter coli.
However, other species have been described (Campylobacter lari, Campylobacter upsaliensis and others).
Note 3 to entry: Campylobacter is usually capable of growth in the selective enrichment media Bolton broth and
Preston broth.
9.4.1
Add the following text after the last paragraph:
NOTE PCR tests for confirmation and species identification are described in Annexes D and E. The results
for the ILS study are described in Annex F.
9.5.1, second sentence
Replace the text with the following:
However, other species have been described (Campylobacter lari, Campylobacter upsaliensis and
others); the characteristics given in Table 2 permit their differentiation from Campylobacter jejuni and
Campylobacter coli.
ISO 10272-2:2017/Amd.1:2023(E)
9.5.1
Add the following text as the second paragraph:
Additionally, Annex E describes molecular methods for identification of thermotolerant Campylobacter
species, which can be used as an alternative to the biochemical identification described in 9.5.2 to 9.5.5.
9.5.4, second paragraph
Replace the text with the following:
If the indoxyl acetate is hydrolysed, a colour change to blue occurs within 5 min to 10 min. If there is an
unclear result after 10 min, a better result can be obtained after waiting for another 20 min. No colour
change indicates hydrolysis has not taken place.
9.5.5, Table 2
Replace the table with the following:
b b
Characteristic C. jejuni C. coli C. lari C. upsaliensis
Catalase (9.5.2) + + + – or weak
a
Hydrolysis of hippurate (9.5.3) + – – –
c
Indoxyl acetate (9.5.4) + + – +
Key
+ = positive
–   = negative
a
Some hippurate-negative C. jejuni strains have been reported.
b
The same characteristics can appear also for other Campylobacter spp.
c
Indoxyl acetate negative C. upsaliensis strains have been reported.
11.1
Add the following text after the first sentence:
The results have been published, see Reference [12].
Clause B.2
Replace the text with the following:
See the ISO 6887 series.
Clause B.9, Table B.1
Replace the table with the following:
ISO 10272-2:2017/Amd.1:2023(E)
Table B.1 — Performance testing of culture media for Campylobacter
Characteristic
Refer- Method
Control WDCM reactions of
b
Medium Function Incubation ence of Criteria
a
strains numbers target
media control
microorganism
Campylobacter  00156 or
Greyish, flat and
c
jejuni 00005
Blood Quantita- moist, sometimes
Productivity P ≥ 0,5
R
agar tive with metallic
Campylobacter  00004 or
sheen
c
coli 00072
(44 ± 4) h/
(41,5 ± 1) °C
Total or
mCCD
Escherichia 00012 or Qualita- partial No characteristic
agar
microaerobic —
c
coli 00013
...