EN ISO 23611-3:2019

Soil quality - Sampling of soil invertebrates - Part 3: Sampling and extraction of enchytraeids (ISO 23611-3:2019)

This document specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms).
Basic information on the ecology of enchytraeids and their use as bioindicators in the terrestrial environment is included in the Bibliography.
This document applies to all terrestrial biotopes in which enchytraeids occur. The sampling design of field studies in general is given in ISO 18400-101. These details can vary according to the climatic/regional conditions of the site to be sampled and an overview on the determination of effects of pollutants on enchytraeids in field situations is given in Reference [6].
Methods for some other soil organism groups such as earthworms or arthropods are given in ISO 23611-1, ISO 23611-2, ISO 23611-4 and ISO 23611-5.
This document is not applicable for very wet or flooded soils and might be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains).
When sampling soil invertebrates, it is highly recommendable to characterize the site (e.g. concerning soil properties, climate and land use). However, such a characterization is not covered by this document. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 are more suitable for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity.

Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 3: Probenahme und Extraktion von Enchytraeen (ISO 23611-3:2019)

Dieses Dokument legt ein Verfahren zur Probenahme, Handhabung und Extraktion von Enchytraeen aus terrestrischen Freilandböden fest, mit dessen Hilfe die Voraussetzungen für die Verwendung dieser Tiere als Bioindikatoren (z. B. zur Beurteilung der Beschaffenheit eines Bodens als Lebensraum für Organismen) geschaffen werden.
Grundlegende Informationen zur Ökologie von Enchytraeen und ihrer Verwendung als Bioindikatoren in der terrestrischen Umwelt lassen sich den im Literaturverzeichnis aufgeführten Quellen entnehmen.
Dieses Dokument gilt für alle terrestrischen Biotope, in denen Enchytraeen leben. Der für Freiland¬unter-suchungen im Allgemeinen gültige Probenahmeplan ist bereits in ISO 18400 101 festgelegt. Diese Einzel-heiten können in Abhängigkeit von den klimatischen/regionalen Bedingungen des zu beprobenden Stand-orts variieren. Verweisung [6] vermittelt einen Überblick zur Bestimmung der Wirkungen von Verun-reinigungen auf Enchytraeen unter Freilandbedingungen.
Die Verfahren für einige andere Bodenorganismengruppen, wie Regenwürmer und Arthropoden, sind in ISO 23611 1, ISO 23611 2, ISO 23611 4 und ISO 23611 5 festgelegt.
Dieses Dokument gilt nicht für nasse oder überflutete Böden und kann unter extremen klimatischen oder geographischen Bedingungen (z. B. im Hochgebirge) schwierig anzu¬wenden sein.
Für die Probenahme von Wirbellosen in Böden wird nachdrücklich empfohlen, den Standort zu charakterisieren (z. B. Berücksichtigung der Bodeneigenschaften, des Klimas und der Bodennutzung). Eine derartige Charakterisierung wird jedoch in diesem Dokument nicht behandelt. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 und ISO 11465 sind zur Bestimmung des pH Wertes, der Korngrößenverteilung, des C/N Verhältnisses, des organischen Kohlenstoffgehalts und des Wasserrückhaltevermögens geeigneter.

Qualité du sol - Prélèvement des invertébrés du sol - Partie 3: Prélèvement et extraction des enchytréides (ISO 23611-3:2019)

Le présent document spécifie une méthode pour le prélèvement, la manipulation et l'extraction des enchytréides du sol prélevés sur le terrain, comme prérequis à l'utilisation de ces animaux en tant que bio-indicateurs (par exemple, pour évaluer la qualité d'un sol en tant qu'habitat pour des organismes).
Les informations de base concernant l'écologie des enchytréides et leur utilisation en tant que bio-indicateurs dans l'environnement terrestre sont disponibles dans la Bibliographie.
Le présent document s'applique à tous les biotopes terrestres dans lesquels des enchytréides sont présents. La conception de l'échantillonnage pour les études sur le terrain est, de manière générale, décrite dans l'ISO 18400‑101. Ces détails peuvent varier en fonction des conditions climatiques/régionales du site sur lequel l'échantillon doit être prélevé et un aperçu de la détermination des effets sur site des polluants vis-à-vis des enchytréides est fourni dans la Référence [6].
Des méthodes applicables à d'autres groupes d'organismes du sol, comme les vers de terre ou les arthropodes, sont données dans les normes ISO 23611‑1, ISO 23611‑2, ISO 23611‑4 et ISO 23611‑5.
Le présent document n'est pas applicable aux sols très humides ou inondés et peut s'avérer difficile à mettre en œuvre dans des conditions climatiques ou géographiques extrêmes (en haute montagne, par exemple).
Lors du prélèvement d'invertébrés du sol, il est vivement recommandé de caractériser le site (par exemple, du point de vue des propriétés du sol, du climat et de l'utilisation du terrain). Cependant, cette caractérisation n'est pas traitée dans le présent document. Les normes ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 et ISO 11465 sont plus appropriées pour le mesurage du pH, de la répartition granulométrique, du rapport C/N, de la teneur en carbone organique et de la capacité de rétention d'eau.

Kakovost tal - Vzorčenje nevretenčarjev v tleh - 3. del: Vzorčenje in ekstrakcija enhitrej iz tal (ISO 23611-3:2019)

Ta standard določa metodo za vzorčenje enhitrejev iz tal na kopenskem terenu ter rokovanje z njimi kot predpogoj za uporabo teh živali kot bioloških indikatorjev (npr. za oceno kakovosti tal kot habitata za organizme). Osnovne informacije o ekologiji enhitrejev in njihovi uporabi kot bioloških indikatorjev v kopenskem okolju so vključene v bibliografiji. Ta dokument se uporablja za vse kopenske biotope, v katerih se pojavljajo enhitreji. Načrtovanje vzorčenja za terenske raziskave na splošno je podano v standardu ISO 18400-101. Te podrobnosti se lahko razlikujejo glede na podnebne/regionalne razmere območja, ki ga je treba vzorčiti, pregled nad ugotavljanjem učinkov onesnaževal na enhitreje v terenskih razmerah pa je naveden v referencah [6]. Metode za nekatere druge skupine talnih organizmov, kot so deževniki ali členonožci, so podane v standardih ISO 23611-1, ISO 23611-2, ISO 23611-4 in ISO 23611-5. Ta dokument se ne uporablja za zelo vlažna ali poplavljena tla, uporaba za ekstremne podnebne ali geografske razmere (npr. v visokogorju) pa je lahko otežena. Pri vzorčenju nevretenčarjev iz tal je zelo priporočljivo opisati območje (npr. lastnosti tal, podnebje in raba tal). Vendar tak opis v tem dokumentu ni zajet. Za merjenje vrednosti pH, porazdelitve velikosti delcev, razmerja C/N, vsebnosti organskega ogljika in zmogljivosti zadrževanja vode so primernejši standardi ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 in ISO 11465.

General Information

• Status: Published
• Publication Date: 01-Oct-2019
• Withdrawal Date: 29-Apr-2020
• ICS: 13.080.05 - Examination of soils in general
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 02-Oct-2019
• Completion Date: 02-Oct-2019
• Ref Project: SIST EN ISO 23611-3:2020 - Soil quality - Sampling of soil invertebrates - Part 3: Sampling and extraction of enchytraeids (ISO 23611-3:2019)

Relations

• Replaces: EN ISO 23611-3:2011 - Soil quality - Sampling of soil invertebrates - Part 3: Sampling and soil extraction of enchytraeids (ISO 23611-3:2007)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2020
Nadomešča:
SIST EN ISO 23611-3:2012
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 3. del: Vzorčenje in ekstrakcija
enhitrej iz tal (ISO 23611-3:2019)
Soil quality - Sampling of soil invertebrates - Part 3: Sampling and extraction of
enchytraeids (ISO 23611-3:2019)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 3: Probenahme
und Bodenextraktion von Enchytraeen (ISO 23611-3:2019)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 3: Prélèvement et extraction
des enchytréides (ISO 23611-3:2019)
Ta slovenski standard je istoveten z: EN ISO 23611-3:2019
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 23611-3
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2019
EUROPÄISCHE NORM
ICS 13.080.05; 13.080.30 Supersedes EN ISO 23611-3:2011
English Version
Soil quality - Sampling of soil invertebrates - Part 3:
Sampling and extraction of enchytraeids (ISO 23611-
3:2019)
Qualité du sol - Prélèvement des invertébrés du sol - Bodenbeschaffenheit - Probenahme von Wirbellosen
Partie 3: Prélèvement et extraction des enchytréides im Boden - Teil 3: Probenahme und Bodenextraktion
(ISO 23611-3:2019) von Enchytraeen (ISO 23611-3:2019)
This European Standard was approved by CEN on 12 August 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-3:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 23611-3:2019) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Test methods for environmental
characterization of solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2020, and conflicting national standards shall be
withdrawn at the latest by April 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23611-3:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 23611-3:2019 has been approved by CEN as EN ISO 23611-3:2019 without any
modification.
INTERNATIONAL ISO
STANDARD 23611-3
Second edition
2019-08
Soil quality — Sampling of soil
invertebrates —
Part 3:
Sampling and extraction of
enchytraeids
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 3: Prélèvement et extraction des enchytréides
Reference number
ISO 23611-3:2019(E)
©
ISO 2019
ISO 23611-3:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
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Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 23611-3:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 2
7 Procedure. 3
7.1 Soil sampling . 3
7.2 Extraction of the enchytraeids . 3
7.3 Microscopic identification . 4
7.4 Preservation of Enchytraeidae . 5
7.5 Validity of the extraction process. 5
7.6 Determination of biomass . 5
8 Data assessment. 5
9 Test report . 6
Annex A (informative) Quick extraction of enchytraeids . 7
Annex B (informative) Examples of the use of soil invertebrates in soil monitoring
programmes (including presentation of their results) . 9
Bibliography .11
ISO 23611-3:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
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World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso
.org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological characterization.
This second edition cancels and replaces the first edition (ISO 23611-3:2007), which has been technically
revised. The main changes to the previous edition are as follows:
— addition of examples of enchytraeid monitoring programmes (including presentation of their
results) as an informative annex.
A list of all parts in the ISO 23611 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved

ISO 23611-3:2019(E)
Introduction
This document has been developed to address a growing need for the standardization of terrestrial
zoological field methods. Such methods, mainly covering the sampling, extraction and handling of soil
invertebrates, are needed for the following purposes:
— biological classification of soils including soil quality assessment (e.g. References [4], [25], [27],
[31], [36]);
— terrestrial bioindication and long-term monitoring (e.g. References [4], [30]);
— evaluation of the effects of chemicals on soil animals (References [18], [26], [28]).
Data for these purposes are gained by standardized methods since they can form the basis for far-
reaching decisions (e.g. whether a given site should be remediated or not). In fact, the lack of such
standardized methods is one of the most important reasons why biological classification concepts in
terrestrial (i.e. soil) habitats have so far been relatively rarely used in comparison with aquatic sites.
Originally, the methods described here were developed for taxonomical and ecological studies,
investigating the role of enchytraeids in various soil ecosystems. These animals without doubt belong
[7]
to the most important soil invertebrates in temperate regions (mainly in acidic soils ). Their influence
[17][23]
on soil functions like litter decomposition and nutrient cycling is well known . Due to their often
very high numbers, and their population biomass, they are also important in many terrestrial food-
[6]
webs . Some species have unintentionally been distributed by humans in ma
...