Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR

This Technical Specification specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya) [1]. A mustard content of 10 mg/kg or greater and a soya content of 10 mg/kg or greater can be detected with a probability of > 95 %.

Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren - Teil 5: Senf (Sinapis alba) sowie Soja (Glycine max) - Qualitativer Nachweis einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time PCR

Diese Technische Spezifikation legt ein Verfahren zum qualitativen Nachweis einer speziesspezifischen DNA von weißem Senf (Sinapis alba) und Soja (Glycine max) in Brühwurst mittels singleplex Real-time PCR auf Basis der Gene MADS-D (Senf) und Lectin (Soja) fest [1]. Ein Senfgehalt von 10 mg/kg oder höher und ein Sojagehalt von 10 mg/kg oder höher können mit einer Wahrscheinlichkeit > 95 % nachgewiesen werden.

Živila - Odkrivanje prisotnosti alergenov v živilih z metodami molekularne biologije - 5. del: Gorčica (Sinapis alba) in soja (Glycine max) - Kvalitativno odkrivanje specifičnega niza DNK v obarjenih klobasah s PCR v realnem času

Ta osnutek tehnične specifikacije določa postopek za kvalitativno odkrivanje specifične DNK iz bele gorčice (Sinapis alba) in soje (Glycine max) v obarjenih klobasah z enojno PCR v realnem času na osnovi genov MADS-D (gorčica) in lektina (soja). Odkriti je mogoče delež gorčice 10 mg/kg ali večji in delež soje 10 mg/kg ali večji z verjetnostjo > 95 %.

General Information

Status
Withdrawn
Publication Date
21-Jun-2016
Withdrawal Date
21-Feb-2023
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
22-Feb-2023

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 15634-5:2016
01-september-2016
äLYLOD2GNULYDQMHSULVRWQRVWLDOHUJHQRYYåLYLOLK]PHWRGDPLPROHNXODUQHELRORJLMH
GHO*RUþLFD 6LQDSLVDOED LQVRMD *O\FLQHPD[ .YDOLWDWLYQRRGNULYDQMH
VSHFLILþQHJDQL]D'1.YREDUMHQLKNOREDVDKV3&5YUHDOQHPþDVX
Foodstuffs - Detection of food allergens by molecular biological methods - Part 5:
Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA
sequence in cooked sausages by real-time PCR
Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen
Verfahren - Teil 5: Senf (Sinapis alba) sowie Soja (Glycine max) - Qualitativer Nachweis
einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time PCR
Ta slovenski standard je istoveten z: CEN/TS 15634-5:2016
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.120.10 Meso in mesni proizvodi Meat and meat products
SIST-TS CEN/TS 15634-5:2016 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST-TS CEN/TS 15634-5:2016

---------------------- Page: 2 ----------------------

SIST-TS CEN/TS 15634-5:2016


CEN/TS 15634-5
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

June 2016
TECHNISCHE SPEZIFIKATION
ICS 07.100.30; 67.120.10
English Version

Foodstuffs - Detection of food allergens by molecular
biological methods - Part 5: Mustard (Sinapis alba) and
soya (Glycine max) - Qualitative detection of a specific
DNA sequence in cooked sausages by real-time PCR
 Lebensmittel - Nachweis von Lebensmittelallergenen
mit molekularbiologischen Verfahren - Teil 5: Senf
(Sinapis alba) sowie Soja (Glycine max) - Qualitativer
Nachweis einer spezifischen DNA-Sequenz in
Brühwürsten mittels Real-time PCR
This Technical Specification (CEN/TS) was approved by CEN on 18 April 2016 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 15634-5:2016 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST-TS CEN/TS 15634-5:2016
CEN/TS 15634-5:2016 (E)
Contents Page
European foreword . 3
1 Scope . 4
2 Principle . 4
3 Reagents . 4
4 Apparatus and equipment . 6
5 Procedure. 6
5.1 General . 6
5.2 Sample preparation . 6
5.3 DNA extraction with CTAB .
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 15634-5:2016
01-februar-2016
äLYLOD2GNULYDQMHSULVRWQRVWLDOHUJHQRYYåLYLOLK]PHWRGDPLPROHNXODUQHELRORJLMH
GHO*RUþLFD 6LQDSLVDOED LQVRMD *O\FLQHPD[ .YDOLWDWLYQRRGNULYDQMH
VSHFLILþQHJDQL]D'1.YREDUMHQLKNOREDVDKV3&5YUHDOQHPþDVX
Foodstuffs - Detection of food allergens by molecular biological methods - Part 5:
Mustard (Sinapis alba) and soya (Glycine max) - Qualitative dectection of a specific DNA
sequence in cooked sausages by real-time PCR
Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen
Verfahren - Teil 5: Senf (Sinapis alba) sowie Soja (Glycine max) - Qualitativer Nachweis
einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time PCR
Ta slovenski standard je istoveten z: FprCEN/TS 15634-5
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.120.10 Meso in mesni proizvodi Meat and meat products
kSIST-TS FprCEN/TS 15634-5:2016 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 15634-5:2016

---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 15634-5:2016


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 15634-5
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

December 2015
ICS 07.100.30; 67.120.10
English Version

Foodstuffs - Detection of food allergens by molecular
biological methods - Part 5: Mustard (Sinapis alba) and
soya (Glycine max) - Qualitative dectection of a specific
DNA sequence in cooked sausages by real-time PCR
 Lebensmittel - Nachweis von Lebensmittelallergenen
mit molekularbiologischen Verfahren - Teil 5: Senf
(Sinapis alba) sowie Soja (Glycine max) - Qualitativer
Nachweis einer spezifischen DNA-Sequenz in
Brühwürsten mittels Real-time PCR


This draft Technical Specification is submitted to CEN members for formal vote. It has been drawn up by the Technical
Committee CEN/TC 275.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 15634-5:2015 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 15634-5:2016
FprCEN/TS 15634-5:2015 (E)
Contents Page
European foreword . 3
1 Scope . 4
2 Principle . 4
3 Reagents . 4
4 Apparatus and equipment . 6
5 Sampling . 6
6 Procedure. 6
6.1 General . 6
6.2 Sample preparation . 7
6.3 DNA extraction with CTAB . 7
6.4 DNA purification by means
...

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