Milk and milk products - Determination of alkaline phosphatase activity - Part 1: Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)

This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks.
This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l.

Milch und Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase - Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816‑1:2024)

Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1: Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2024)

Le présent document spécifie une méthode fluorimétrique pour la détermination de l’activité de la phosphatase alcaline (ALP) (EC 3.1.3.1) dans les laits crus et traités thermiquement entiers, demi‑écrémés, écrémés et les laits aromatisés.
La méthode est applicable au lait et aux boissons à base de lait de vache, de brebis et de chèvre. Elle est également applicable au lait en poudre après reconstitution.
L’instrument utilisé pour la détermination de l’ALP peut lire des activités jusqu’à 7 000 mU/l (milliunités par litre). Si l’activité est supérieure à 7 000 mU/l, une dilution avec du lait exempt d’ALP est effectuée de façon à obtenir un mesurage ne dépassant pas 7 000 mU/l.

Mleko in mlečni izdelki- Določanje aktivnosti alkalne fosfataze - 1. del: Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2024)

Ta dokument določa fluorimetrijsko metodo za določanje aktivnosti alkalne fosfataze (ALP) (EC
3.1.3.1) v surovem in toplotno obdelanem polnomastnem, polmastnem in posnetem mleku in mleku z okusom.
Ta metoda se uporablja za mleko in pijače na osnovi kravjega, ovčjega in kozjega mleka. Uporablja se tudi za mleko v prahu po pripravi.
Instrument, ki se uporablja za določanje alkalne fosfataze, lahko beleži aktivnosti do 7000 milienot na liter (mU/l).
Če je aktivnost višja od 7000 mU/l, se razredči z mlekom brez vsebnosti alkalne fosfataze, da se pridobi meritev, ki ne presega 7000 mU/l.

General Information

Status
Published
Publication Date
16-Jan-2024
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
17-Jan-2024
Completion Date
17-Jan-2024

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SLOVENSKI STANDARD
SIST EN ISO 11816-1:2024
01-marec-2024
Nadomešča:
SIST EN ISO 11816-1:2014
Mleko in mlečni proizvodi - Določanje aktivnosti alkalne fosfataze - 1. del:
Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 1:
Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)
Milch und Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase -
Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816-
1:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1:
Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-1:2024
ICS:
67.100.10 Mleko in predelani mlečni Milk and processed milk
proizvodi products
SIST EN ISO 11816-1:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 11816-1:2024

---------------------- Page: 2 ----------------------
SIST EN ISO 11816-1:2024


EN ISO 11816-1
EUROPEAN STANDARD

NORME EUROPÉENNE

January 2024
EUROPÄISCHE NORM
ICS 67.100.10 Supersedes EN ISO 11816-1:2013
English Version

Milk and milk products - Determination of alkaline
phosphatase activity - Part 1: Fluorimetric method for milk
and milk-based drinks (ISO 11816-1:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milcherzeugnisse - Bestimmung der
la phosphatase alcaline - Partie 1: Méthode Aktivität der alkalischen Phosphatase - Teil 1:
fluorimétrique pour le lait et les boissons à base de lait Fluorimetrisches Verfahren für Milch und flüssige
(ISO 11816-1:2024) Milchprodukte (ISO 11816-1:2024)
This European Standard was approved by CEN on 25 March 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-1:2024 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 11816-1:2024
EN ISO 11816-1:2024 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 11816-1:2024
EN ISO 11816-1:2024 (E)
European foreword
This document (EN ISO 11816-1:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-1:2013.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, F
...

SLOVENSKI STANDARD
SIST EN ISO 11816-1:2024
01-marec-2024
Nadomešča:
SIST EN ISO 11816-1:2014
Mleko in mlečni izdelki- Določanje aktivnosti alkalne fosfataze - 1. del:
Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 1:
Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)
Milch und Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase -
Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816-
1:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1:
Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-1:2024
ICS:
67.100.10 Mleko in predelani mlečni Milk and processed milk
proizvodi products
SIST EN ISO 11816-1:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 11816-1:2024

---------------------- Page: 2 ----------------------
SIST EN ISO 11816-1:2024


EN ISO 11816-1
EUROPEAN STANDARD

NORME EUROPÉENNE

January 2024
EUROPÄISCHE NORM
ICS 67.100.10 Supersedes EN ISO 11816-1:2013
English Version

Milk and milk products - Determination of alkaline
phosphatase activity - Part 1: Fluorimetric method for milk
and milk-based drinks (ISO 11816-1:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milcherzeugnisse - Bestimmung der
la phosphatase alcaline - Partie 1: Méthode Aktivität der alkalischen Phosphatase - Teil 1:
fluorimétrique pour le lait et les boissons à base de lait Fluorimetrisches Verfahren für Milch und flüssige
(ISO 11816-1:2024) Milchprodukte (ISO 11816-1:2024)
This European Standard was approved by CEN on 25 March 2023.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-1:2024 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 11816-1:2024
EN ISO 11816-1:2024 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 11816-1:2024
EN ISO 11816-1:2024 (E)
European foreword
This document (EN ISO 11816-1:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-1:2013.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Fran
...

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