EN 15784:2021

Animal feeding stuffs: Methods of sampling and analysis - Detection and enumeration of Bacillus spp. used as feed additive

This document specifies general rules for the enumeration of bacilli in feeding stuffs (additives, premixtures and compound feeds including mineral feeds) [4] that contain bacilli as a single microorganism component or in a mixture with other microorganisms. There are different categories of feed samples:
a)   Additives containing about 1010 colony forming units (CFU)/g;
b)   Premixtures containing about 1011 CFU/kg;
c)   Compound feeds, meal or pellets containing about 109 CFU/kg.

Futtermittel: Probenahme- und Untersuchungsverfahren - Nachweis und Zählung von Bacillus spp. als Futtermittelzusatzstoff

Dieses Dokument legt allgemeine Regeln für die Zählung von Bazillen in Futtermitteln (Zusatzstoffe, Vor-mischungen und Mischfuttermittel einschließlich mineralischer Futtermittel) [4] fest, die Bazillen als einzigen mikrobiellen Bestandteil oder in einem Gemisch mit anderen Mikroorganismen enthalten. Es gibt unter¬schiedliche Kategorien von Futtermittelproben:
a)   Zusatzstoffe, die etwa 1010 koloniebildende Einheiten (KbE)/g enthalten;
b)   Vormischungen, die etwa 1011 KbE/kg enthalten;
c)   Mischfuttermittel, Mehle oder Pellets, die etwa 109 KbE/kg enthalten.

Aliments des animaux: Méthodes d’échantillonnage et d’analyse - Détection et dénombrement des souches de Bacillus spp. utilisées comme additifs pour l’alimentation animale

Le présent document spécifie des règles générales pour le dénombrement des Bacillus présents dans les aliments pour animaux (additifs, prémélanges et aliments composés, y compris les aliments minéraux) [4] qui contiennent des Bacillus comme seul micro-organisme constitutif ou en mélange avec d’autres micro organismes. Il existe différentes catégories d’échantillons d’aliments pour animaux :
a)   les additifs contenant environ 1010 UFC/g (UFC : unités formant colonie) ;
b)   les prémélanges contenant environ 1011 UFC/kg ;
c)   les aliments composés, farines ou granulés contenant environ 109 UFC/kg.

Krma: Metode vzorčenja in analize - Določanje in štetje prisotnih Bacillus spp. uporabljen kot krmni dodatek

Ta evropski standard določa splošna pravila za štetje probiotičnih bacilov v krmi, ki vsebuje bacile (vrste Bacillus) kot posamezen mikroorganizem, komponento ali pomešano z drugimi mikroorganizmi. Ta metoda se ne uporablja za mineralno krmo, ki je opredeljena kot dopolnilna krma, sestavljena predvsem iz mineralov, in vsebuje najmanj 40 % surovega pepela (Direktiva Sveta 79/373/EGS).
Obstajajo različne kategorije vzorcev krme:
a)   dodatki, ki vsebujejo približno 10+10 enot, ki tvorijo kolonije (CFU)/g;
b)   premiksi, ki vsebujejo približno 10+8 CFU/g;
c)   krma, moka ali peleti, ki vsebujejo približno 10+6 CFU/g ter vključujejo popolno krmo in mlečne nadomestke.
Meje zaznavanja so 500 (5 × 10+2) enot, ki tvorijo kolonije na gram (CFU/g). Meje določanja so 2 × 10+4 CFU/g.

General Information

• Status: Published
• Publication Date: 23-Nov-2021
• Withdrawal Date: 30-May-2022
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
• Drafting Committee: CEN/TC 327/WG 3 - Feed additives and drugs
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 24-Nov-2021
• Due Date: 05-Jul-2020
• Completion Date: 24-Nov-2021
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Ref Project: SIST EN 15784:2022 - Animal feeding stuffs: Methods of sampling and analysis - Detection and enumeration of Bacillus spp. used as feed additive

Relations

• Replaces: EN 15784:2009 - Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp.
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 15784:2022
01-februar-2022
Nadomešča:
SIST EN 15784:2009
Krma: Metode vzorčenja in analize - Določanje in štetje prisotnih Bacillus spp.
uporabljen kot krmni dodatek
Animal feeding stuffs: Methods of sampling and analysis - Detection and enumeration of
Bacillus spp. used as feed additive
Futtermittel: Probenahme- und Untersuchungsverfahren - Nachweis und Zählung von
Bacillus spp. als Futtermittelzusatzstoff
Aliments des animaux: Méthodes d’échantillonnage et d’analyse - Détection et
dénombrement des souches de Bacillus spp. utilisées comme additifs pour l’alimentation
animale
Ta slovenski standard je istoveten z: EN 15784:2021
ICS:
65.120 Krmila Animal feeding stuffs
SIST EN 15784:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 15784:2022

---------------------- Page: 2 ----------------------
SIST EN 15784:2022


EN 15784
EUROPEAN STANDARD

NORME EUROPÉENNE

November 2021
EUROPÄISCHE NORM
ICS 65.120 Supersedes EN 15784:2009
English Version

Animal feeding stuffs: Methods of sampling and analysis -
Detection and enumeration of Bacillus spp. used as feed
additive
Aliments des animaux: Méthodes d'échantillonnage et Futtermittel: Probenahme- und
d'analyse - Détection et dénombrement des souches de Untersuchungsverfahren - Nachweis und Zählung von
Bacillus spp. utilisées comme additifs pour Bacillus spp. als Futtermittelzusatzstoff
l'alimentation animale
This European Standard was approved by CEN on 2 August 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15784:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 15784:2022
EN 15784:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Diluent and culture medium . 6
5.1 Diluent . 6
5.2 Culture medium tryptone soy agar (TSA) . 6
6 Apparatus . 7
7 Sampling . 8
8 Prepar
...