EN 15634-5:2023

Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR

This document specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya).

Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren - Teil 5: Senf (Sinapis alba) sowie Soja (Glycine max) - Qualitativer Nachweis einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time PCR

Dieses Dokument legt ein Verfahren zum qualitativen Nachweis einer speziesspezifischen DNA von weißem Senf (Sinapis alba) und Soja (Glycine max) in Brühwurst mittels Singleplex-Real time-PCR auf Basis der Gene MADS D (Senf) und Lectin (Soja) fest.

Produits alimentaires - Détection des allergènes alimentaires par des méthodes d’analyse de biologie moléculaire - Partie 5 : Moutarde (Sinapis alba) et soja (Glycine max) - Détection qualitative d’une séquence d’ADN spécifique dans des saucisses cuites, par PCR en temps réel

Le présent document décrit une méthode de détection qualitative d´ADN spécifique de la moutarde blanche (Sinapis alba) et du soja (Glycine max) dans des saucisses cuites, par PCR (réaction de polymérisation en chaîne) singleplex en temps réel reposant sur les gènes de MADS-D (moutarde) et de lectine (soja).

Živila - Določevanje alergenov v živilih z molekularno biološkimi metodami - 5. del: Gorčica (Sinapis alba) in soja (Glycine max) - Kvalitativno določanje specifičnega zaporedja DNK v obarjenih klobasah s PCR v realnem času

Ta metoda določa postopek za kvalitativno odkrivanje specifične DNK iz bele gorčice (Sinapis alba) in soje (Glycine max) v obarjenih klobasah z enojno verižno reakcijo s polimerazo v realnem času na osnovi genov MADS-D (gorčica) in lektina (soja). Odkriti je mogoče delež gorčice 10 mg/kg ali večji in delež soje 10 mg/kg ali več z verjetnostjo > 95 %.

General Information

• Status: Published
• Publication Date: 21-Feb-2023
• ICS: 07.100.30 - Food microbiology
• ICS: 67.120.10 - Meat and meat products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 12 - Food allergens
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 22-Feb-2023
• Due Date: 15-Aug-2022
• Completion Date: 22-Feb-2023
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Ref Project: SIST EN 15634-5:2023 - Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR

Relations

• Replaces: CEN/TS 15634-5:2016 - Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR
• Effective Date: 03-Jun-2020

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 15634-5:2023
01-maj-2023
Živila - Določevanje alergenov v živilih z molekularno biološkimi metodami - 5. del:
Gorčica (Sinapis alba) in soja (Glycine max) - Kvalitativno določanje specifičnega
zaporedja DNK v obarjenih klobasah s PCR v realnem času
Foodstuffs - Detection of food allergens by molecular biological methods - Part 5:
Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA
sequence in cooked sausages by real-time PCR
Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen
Verfahren - Teil 5: Senf (Sinapis alba) sowie Soja (Glycine max) - Qualitativer Nachweis
einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time PCR
Produits alimentaires - Détection des allergènes alimentaires par des méthodes
d’analyse de biologie moléculaire - Partie 5 : Moutarde (Sinapis alba) et soja (Glycine
max) - Détection qualitative d’une séquence d’ADN spécifique dans des saucisses
cuites, par PCR en temps réel
Ta slovenski standard je istoveten z: EN 15634-5:2023
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.120.10 Meso in mesni proizvodi Meat and meat products
SIST EN 15634-5:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 15634-5:2023

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SIST EN 15634-5:2023
EN 15634-5
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2023
EUROPÄISCHE NORM
ICS 07.100.30; 67.120.10 Supersedes CEN/TS 15634-5:2016
English Version
Foodstuffs - Detection of food allergens by molecular
biological methods - Part 5: Mustard (Sinapis alba) and
soya (Glycine max) - Qualitative detection of a specific
DNA sequence in cooked sausages by real-time PCR
Produits alimentaires - Détection des allergènes Lebensmittel - Nachweis von Lebensmittelallergenen
alimentaires par des méthodes d'analyse de biologie mit molekularbiologischen Verfahren - Teil 5: Senf
moléculaire - Partie 5 : Moutarde (Sinapis alba) et soja (Sinapis alba) sowie Soja (Glycine max) - Qualitativer
(Glycine max) - Détection qualitative d'une séquence Nachweis einer spezifischen DNA-Sequenz in
d'ADN spécifique dans des saucisses cuites, par PCR en Brühwürsten mittels Real-time PCR
temps réel
This European Standard was approved by CEN on 16 January 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
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EUROPÄISCHES KOMITEE FÜR NORMUNG
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© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15634-5:2023 E
worldwide for CEN national Members.

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SIST EN 15634-5:2023
EN 15634-5:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle .
...