EN ISO 10253:2024

Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum (ISO 10253:2024)

This document specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.).
The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.
NOTE            With modifications, as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metal compounds, effluents, marine water samples and elutriates of sediments can be tested.

Wasserbeschaffenheit - Wachstumshemmtest mit marinen Algen Skeletonema sp. und Phaeodactylum tricornutum (ISO 10253:2024)

Dieses Dokument legt ein Verfahren zur Bestimmung der Wachstumshemmung der einzelligen marinen Algen-Spezies Skeletonema sp. und Phaeodactylum tricornutum durch im Meerwasser enthaltene Substanzen und Substanzgemische oder durch Umweltwasserproben (Abwasser, Eluate usw.) fest.
Das Verfahren kann für die Untersuchung von leicht wasserlöslichen Substanzen angewendet werden, die durch das Testmedium weder signifikant abgebaut noch in irgendeiner anderen Weise aus diesem eliminiert werden.
ANMERKUNG   Mit Änderungen, wie sie in ISO 14442 und ISO 5667 16 beschrieben sind, kann das Verfahren auch zur Bestimmung der Hemmwirkung von schwer wasserlöslichen organischen und anorganischen Stoffen, flüchtigen Verbindungen, Metallverbindungen, Abwässern, Meerwasserproben und Eluaten von Sedimenten.
WARNUNG — Anwender dieses Dokuments sollten mit der üblichen Laborpraxis vertraut sein. Dieses Dokument gibt nicht vor, alle unter Umständen mit der Anwendung des Verfahrens verbundenen Sicherheitsaspekte anzusprechen. Es liegt in der Verantwortung des Arbeitgebers, angemessene Sicherheits- und Schutzmaßnahmen zu treffen und sicherzustellen, dass diese mit nationalen Festlegungen übereinstimmen.
WICHTIG — Es ist erforderlich, bei den Untersuchungen nach diesem Dokument Fachleute oder Facheinrichtungen einzuschalten.

Qualité de l'eau - Essai d'inhibition de la croissance des algues marines avec Skeletonema sp. et Phaeodactylum tricornutum (ISO 10253:2024)

Le présent document spécifie une méthode de détermination de l’inhibition de la croissance des algues marines unicellulaires Skeletonema sp. et Phaeodactylum tricornutum provoquée par des substances et des mélanges présents dans l’eau de mer ou par des échantillons d’eau environnementale (effluents, élutriats, etc.).
Cette méthode peut être utilisée pour soumettre à l’essai des substances facilement solubles dans l’eau et qui ne sont pas sensiblement dégradées ou éliminées d’autre manière du milieu d’essai.
NOTE            En appliquant les modifications décrites dans l’ISO 14442 et l’ISO 5667–16, il est possible d’évaluer par cet essai les effets inhibiteurs des matières organiques et inorganiques peu solubles, des composés volatils, des composés métalliques, des effluents, des échantillons d’eaux marines et des élutriats de sédiments.

Kakovost vode - Preskus zaviranja rasti morskih alg s Skeletonema sp. in Phaeodactylum tricornutum (ISO 10253:2024)

General Information

• Status: Published
• Publication Date: 23-Jul-2024
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 24-Jul-2024
• Completion Date: 24-Jul-2024
• Ref Project: SIST EN ISO 10253:2024 - Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum (ISO 10253:2024)

Relations

• Replaces: EN ISO 10253:2016 - Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum (ISO 10253:2016)
• Effective Date: 18-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2024
Nadomešča:
SIST EN ISO 10253:2017
Kakovost vode - Preskus zaviranja rasti morskih alg s Skeletonema sp. in
Phaeodactylum tricornutum (ISO 10253:2024)
Water quality - Marine algal growth inhibition test with Skeletonema sp. and
Phaeodactylum tricornutum (ISO 10253:2024)
Wasserbeschaffenheit - Wachstumshemmtest mit marinen Algen Skeletonema sp. und
Phaeodactylum tricornutum (ISO 10253:2024)
Qualité de l'eau - Essai d'inhibition de la croissance des algues marines avec
Skeletonema sp. et Phaeodactylum tricornutum (ISO 10253:2024)
Ta slovenski standard je istoveten z: EN ISO 10253:2024
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10253
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2024
EUROPÄISCHE NORM
ICS 13.060.70 Supersedes EN ISO 10253:2016
English Version
Water quality - Marine algal growth inhibition test with
Skeletonema sp. and Phaeodactylum tricornutum (ISO
10253:2024)
Qualité de l'eau - Essai d'inhibition de la croissance des Wasserbeschaffenheit - Wachstumshemmtest mit
algues marines avec Skeletonema sp. et Phaeodactylum marinen Algen Skeletonema sp. und Phaeodactylum
tricornutum (ISO 10253:2024) tricornutum (ISO 10253:2024)
This European Standard was approved by CEN on 29 June 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10253:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 10253:2024) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2025, and conflicting national standards shall
be withdrawn at the latest by January 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 10253:2016.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 10253:2024 has been approved by CEN as EN ISO 10253:2024 without any modification.

International
Standard
ISO 10253
Fourth edition
Water quality — Marine algal
2024-07
growth inhibition test with
Skeletonema sp. and Phaeodactylum
tricornutum
Qualité de l’eau — Essai d’inhibition de la croissance des algues
marines avec Skeletonema sp. et Phaeodactylum tricornutum
Reference number
ISO 10253:2024(en) © ISO 2024
ISO 10253:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 10253:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Materials . 2
5.1 Test organisms .2
5.2 Reagents .4
5.2.1 Water .4
5.2.2 Hydrochloric acid or sodium hydroxide solution .4
5.2.3 Synthetic sea water .4
5.2.4 Nutrient stock solution .4
6 Apparatus . 5
7 Procedure . 6
7.1 Preparation of growth medium.6
7.2 Preparation of pre-culture and inoculum .6
7.3 Choice of test concentrations .6
7.4 Preparation of test substance stock solutions .6
7.5 Preparation of test and control batches .7
7.6 Incubation .7
7.7 Measurements .8
8 Validity criteria . 8
9 Interpretation of data . 8
9.1 Plotting growth curves .8
9.2 Calculation of percentage inhibition .8
9.3 Determination of EC(r) .9
x
10 Expression of results . 9
11 Interpretation of results . . 9
12 Test report . 10
Annex A (informative) Preparation of dilution series of mixtures in sea water(effluents or
elutriates) .11
Annex B (informative) Test procedure starting from stored algal inocula, and with direct
measurement of algal growth in spectrophotometric cells .12
Annex C (informative) Performance data .18
Annex D (informative) Marine algal growth inhibition test with Phaeodactylum tricornutum
applied in 24-well microwell plates . 19
Bibliography .26

iii
ISO 10253:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document
...