ISO 22992-2:2020

Textiles - Determination of certain preservatives - Part 2: Determination of triclosan residues method using LC-MS/MS

This document specifies a method for determination of triclosan residues in textiles by high performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS). This method is applicable to all kinds of textile products.

Textiles — Détermination de certains conservateurs — Partie 2: Détermination des résidus de triclosan par une méthode utilisant LC-MS/MS

General Information

• Status: Published
• Publication Date: 29-Oct-2020
• ICS: 59.060.01 - Textile fibres in general
• Technical Committee: ISO/TC 38 - Textiles
• Drafting Committee: ISO/TC 38/WG 22 - Composition and chemical testing
• Current Stage: 9020 - International Standard under periodical review
• Start Date: 15-Oct-2025
• Completion Date: 15-Oct-2025

Overview

ISO 22992-2:2020 specifies a validated laboratory method for the determination of triclosan residues in textiles using liquid chromatography coupled with tandem mass spectrometry (HPLC‑/LC‑MS/MS). The procedure covers sample preparation, ultrasonic extraction with methanol, concentration and cleanup, instrumental analysis by LC‑MS/MS (electrospray ionization, MRM), calibration, blank control and result calculation. The method is applicable to all kinds of textile products.

Key topics and technical requirements

• Scope: Determination of triclosan (CAS 3380‑34‑5) in textile samples; applicable to all textile types.
• Sample preparation: Representative specimen cut to <5 mm pieces; typical test mass (1.00 ± 0.01) g.
• Extraction: Two-step ultrasonic extraction with methanol (25 mL each) at ≈40 °C for (30 ± 2) min; combined extracts concentrated with rotary evaporator to ≈1 mL, dried with nitrogen, reconstituted to 2 mL and filtered (0.22 µm).
• Standards & calibration: Stock standard 1 000 mg/L in methanol; working solutions prepared into at least five calibration levels; quantification by external standard method. Stock stored 0–4 °C up to 6 months; working solutions up to 3 months.
• LC‑MS/MS parameters (Annex A examples): C18 reversed‑phase column (150×2.1 mm, 3.5 µm); mobile phase methanol:water (90:10 v/v); flow 0.25 mL/min; injection 20 µL. ESI negative ion mode, MRM detection; typical precursor m/z 286.7 with transitions to m/z 35.0 (quant) and 142.1. Typical detection limit shown as 0.5 mg/kg.
• Quality control: Blank test to check contamination; dilution or increased sample mass recommended if analyte is outside linear range.
• Reporting & calculation: Results expressed as mg/kg (mass ratio triclosan/test specimen); report must reference ISO 22992‑2:2020 and note any deviations.

Practical applications and users

• Textile testing laboratories implementing regulatory or customer-driven chemical safety checks for triclosan in garments, home textiles and technical textiles.
• Manufacturers and suppliers verifying compliance, screening incoming materials or validating finishing processes that may use preservatives.
• Regulatory bodies and compliance auditors assessing conformity with chemical-restriction requirements.
• Research labs studying triclosan occurrence, migration or treatment effects in textile matrices.
  Keywords: ISO 22992-2:2020, triclosan residues, textiles, HPLC‑MS/MS, LC‑MS/MS, ultrasonic extraction, MRM.

Related standards

• ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
• ISO 22992 series - other parts address additional preservatives and methods (see ISO catalogue for full series).

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 22992-2
First edition
2020-10
Textiles — Determination of certain
preservatives —
Part 2:
Determination of triclosan residues
method using LC-MS/MS
Textiles — Détermination de certains conservateurs —
Partie 2: Détermination des résidus de triclosan par une méthode
utilisant LC-MS/MS
Reference number
©
ISO 2020
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Published in Switzerland
ii © ISO 2020 – All rights reserved

Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus and materials. 2
7 Test procedure . 2
7.1 Preparation of standard solution . 2
7.1.1 Standard stock solution . 2
7.1.2 Working solution . 2
7.2 Preparation of test specimen . 2
7.3 Ultrasonic wave extraction . 2
7.4 Determination of triclosan . . 3
8 Blank test . 3
9 Calculation . 3
10 Test report . 3
Annex A (informative) Test parameters by HPLC-MS/MS . 4
Annex B (informative) Statistical data of interlaboratory trial . 6
Foreword
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This document was prepared by Technical Committee ISO/TC 38, Textiles.
A list of all parts in the ISO 22992 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
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iv © ISO 2020 – All rights reserved

INTERNATIONAL STANDARD ISO 22992-2:2020(E)
Textiles — Determination of certain preservatives —
Part 2:
Determination of triclosan residues method using LC-MS/
MS
WARNING — This document calls for the use of substances and/or procedures that can be
injurious to health if adequate precautions are not taken. It refers only to technical suitability
and does not absolve the user from legal obligations relating to health and safety at any stage.
It has been assumed in the drafting of this document that the execution of its provisions is
entrusted to appropriately qualified and experienced people.
1 Scope
This document specifies a method for determination of triclosan residues in
...