ISO 11050:2020
(Main)Wheat flour and durum wheat semolina — Determination of impurities of animal origin
Wheat flour and durum wheat semolina — Determination of impurities of animal origin
This document specifies a method for determining the content of impurities of animal origin in wheat flours, with or without additives and having an ash yield not exceeding a mass fraction of 0,75 %, and in durum wheat semolinas. This method permits the separation and quantification of contamination of animal origin, such as insects at all stages of their development and their fragments, rodent hairs and their fragments, and mites.
Farines de blé tendre et semoules de blé dur — Détermination des impuretés d'origine animale
Le présent document spécifie une méthode de détermination des impuretés d'origine animale dans les farines de blé tendre, avec ou sans additif, ayant un taux de cendres inférieur ou égal à 0,75 % en fraction massique, et dans les semoules de blé dur. Cette méthode permet de séparer et de dénombrer les souillures d'origine animale, telles que les insectes à tous les stades de leur développement et leurs fragments, les poils de rongeurs et leurs fragments, ainsi que les acariens.
Pšenična moka in pšenični zdrob durum - Določevanje nečistoč živalskega izvora
General Information
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Buy Standard
Standards Content (Sample)
SLOVENSKI STANDARD
SIST ISO 11050:2020
01-december-2020
Nadomešča:
SIST ISO 11050:1997
Pšenična moka in pšenični zdrob durum - Določevanje nečistoč živalskega izvora
Wheat flour and durum wheat semolina -- Determination of impurities of animal origin
Farines de blé tendre et semoules de blé dur -- Détermination des impuretés d'origine
animale
Ta slovenski standard je istoveten z: ISO 11050:2020
ICS:
67.060 Žita, stročnice in proizvodi iz Cereals, pulses and derived
njih products
SIST ISO 11050:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST ISO 11050:2020
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SIST ISO 11050:2020
INTERNATIONAL ISO
STANDARD 11050
Second edition
2020-04
Wheat flour and durum wheat
semolina — Determination of
impurities of animal origin
Farines de blé tendre et semoules de blé dur — Détermination des
impuretés d'origine animale
Reference number
ISO 11050:2020(E)
©
ISO 2020
---------------------- Page: 3 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
---------------------- Page: 4 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents . 3
6 Apparatus . 3
7 Sampling . 5
8 Procedure. 6
8.1 Test portion . 6
8.2 Hydrolysis . 6
8.3 Separation of impurities . 6
8.4 Filtration . 7
8.5 Microscopic examination . 7
8.6 Number of determinations . 8
9 Expression of results . 8
10 Repeatability . 8
11 Test report . 8
Annex A (informative) Characteristics of fragments found on the filters . 9
Annex B (informative) Test report — Determination of animal impurities in accordance
with this document .11
Annex C (informative) Diagram of the procedure .12
Annex D (informative) Chronology of operations and timetable .14
Bibliography .15
© ISO 2020 – All rights reserved iii
---------------------- Page: 5 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4,
Cereals and pulses.
This second edition cancels and replaces the first edition (ISO 11050:1993), which has been technically
revised. The main changes compared with the previous edition are as follows:
— the Scope has been widened;
— the protocol has been improved to make it easier;
— the figures have been updated.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved
---------------------- Page: 6 ----------------------
SIST ISO 11050:2020
INTERNATIONAL STANDARD ISO 11050:2020(E)
Wheat flour and durum wheat semolina — Determination
of impurities of animal origin
1 Scope
This document specifies a method for determining the content of impurities of animal origin in wheat
flours, with or without additives and having an ash yield not exceeding a mass fraction of 0,75 %, and in
durum wheat semolinas.
This method permits the separation and quantification of contamination of animal origin, such as insects
at all stages of their development and their fragments, rodent hairs and their fragments, and mites.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
impurity of animal origin
matter of animal origin [larvae, nymphs or adults of insects (3.10) and their fragments, rodent hairs
and their fragments, mites (3.3)] separated from the product under the conditions specified in this
document
3.2
abdomen
rear part of the body of an insect (3.10), excluding the head and thorax, commonly with eight or more
segments when complete
3.3
mite
very small arthropod belonging to the class Arachnida, often living in large numbers
3.4
appendice
distinctly differentiated prolongation of the body of an arthropod
EXAMPLE Legs, wings, antennae, urogomphi (3.16).
3.5
cephalic capsule
head capsule
sclerous part of an exuvia that once contained the head of a larva
© ISO 2020 – All rights reserved 1
---------------------- Page: 7 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
3.6
caterpillar
larvae of Lepidoptera spp.
Note 1 to entry: Butterfly or moth is the adult stage and chrysalis is the pupal stage.
3.7
scale
bristle (3.13) that has evolved into a flat structure resembling a fish scale and that covers the parts of
the body of certain insects (3.10), in particular the wings of Lepidoptera spp.
3.8
wing case
elytron
hardened front wing of Coleoptera spp.
Note 1 to entry: It is used as a fixed wing in flight and as protective cover for the membranous hind wing.
3.9
false leg
proleg
fleshy extension of the lower part of the abdomen (3.2) of some larvae, sometimes with a crown of fine
hooks (crochets) of chitin
Note 1 to entry: These help with attachment to the substrate, and in movement. Lepidopterous larvae have at
least two pairs of false legs, towards the rear of the body.
3.10
insect
class of animals within the phylum Arthropoda, some of which are recognized pests of stored foodstuffs
3.11
mandible
toughened (sclerotized) mouthpart of insects (3.10)
Note 1 to entry: It is used for the dilaceration or grinding of food.
3.12
pericarp
external envelope of seeds that forms the bran after the grain has been crushed and the flour separated
3.13
bristle
fine but stiff hair of any length present on the cuticle of insects (3.10)
Note 1 to entry: Sensory hairs are called “setae”.
3.14
stage
state of development of an insect (3.10) or mite (3.3)
EXAMPLE Egg, larva, nymph, pupa, adult.
3.15
juvenile stage
pre-adult stage (3.14) of insects (3.10)
EXAMPLE Egg, larva, nymph, pupa.
Note 1 to entry: This term is most often applied to the active stages of larva and nymph.
2 © ISO 2020 – All rights reserved
---------------------- Page: 8 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
3.16
urogomphi
pointed extensions of the cuticle of the final abdominal segment of some insect larvae
Note 1 to entry: They are common, and sometimes diagnostic, features of many Coleoptera spp.
Note 2 to entry: The abdominal extensions of a cockroach are called “cerci”.
4 Principle
Hydrolysis of a test portion with a solution of hydrochloric acid at boiling point. Concentration of the
insoluble particles (impurities other than those of animal origin may be present) at a water/oil interface.
Separation by filtration on a filter paper, microscopic examination and counting under reflected light of
the impurities of animal origin.
5 Reagents
Only use reagents of a recognized analytical grade and demineralized water or water of equivalent purity.
5.1 Ethanol, a volume fraction of 95 %.
5.2 Ethanol solution, a volume fraction of 50 %.
5.3 Ethanol/glycerol, 1 + 1 mixture by volume.
5.4 Hydrochloric acid solution, concentrated at 35 % to 37 %.
5.5 Paraffin oil (known as “Vaseline oil”), fluid, having a viscosity not exceeding 60 mPa·s at 20 °C.
5.6 Liquid detergent, non-foaming.
1)
EXAMPLE Extran, Biodeck 4, Decon 90.
5.7 Liquid detergent, a volume fraction of 1 % aqueous solution of the detergent (5.6) in a
washing bottle.
5.8 Dearomatized white spirit (possible use).
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
1) These are examples of suitable products available commercially. This information is given for the convenience
of users of this document and does not constitute an endorsement by ISO of these products.
© ISO 2020 – All rights reserved 3
---------------------- Page: 9 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
6.1 Separating funnels, conical, of 1 000 ml capacity, fitted with a non-lubricated
polytetrafluoroethylene (PTFE) tap. See the recommended set-up shown in Figure 1.
Key
1 light hydrocarbon phase 3 “heavy” aqueous phase
2 support ring 4 PTFE tap
Figure 1 — Separation apparatus
2)
6.2 Tall-form beaker, of at least 800 ml capacity, fitted with a watch glass made of Pyrex® or PFTE
and of appropriate dimensions to serve as a lid.
6.3 Crystallizing dish or pan, of at least 5 l capacity and of a height slightly less than that of the beaker
(6.2), suitable for use as a cooling bath.
6.4 Graduated cylinders, of 25 ml, 50 ml and 500 ml capacity.
6.5 Washing bottles, with a flexible tube.
2) Pyrex® is the trademark of a product registered by Corning Inc. This information is given for the convenience of
users of this document and does not constitute an endorsement by ISO of the product named. Equivalent products
may be used if they can be shown to lead to the same results.
4 © ISO 2020 – All rights reserved
---------------------- Page: 10 ----------------------
SIST ISO 11050:2020
ISO 11050:2020(E)
3)
6.6 Filter paper , ash free, with rapid filtration characteristics, of 90 mm diameter, corresponding
to that of the filtration unit (6.7), on which fine parallel lines are drawn, spaced 5 mm apart, using a
lead pencil.
6.7 Filtration unit, of the Büchner funnel type, removable, suitable for a
...
INTERNATIONAL ISO
STANDARD 11050
Second edition
2020-04
Wheat flour and durum wheat
semolina — Determination of
impurities of animal origin
Farines de blé tendre et semoules de blé dur — Détermination des
impuretés d'origine animale
Reference number
ISO 11050:2020(E)
©
ISO 2020
---------------------- Page: 1 ----------------------
ISO 11050:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
---------------------- Page: 2 ----------------------
ISO 11050:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents . 3
6 Apparatus . 3
7 Sampling . 5
8 Procedure. 6
8.1 Test portion . 6
8.2 Hydrolysis . 6
8.3 Separation of impurities . 6
8.4 Filtration . 7
8.5 Microscopic examination . 7
8.6 Number of determinations . 8
9 Expression of results . 8
10 Repeatability . 8
11 Test report . 8
Annex A (informative) Characteristics of fragments found on the filters . 9
Annex B (informative) Test report — Determination of animal impurities in accordance
with this document .11
Annex C (informative) Diagram of the procedure .12
Annex D (informative) Chronology of operations and timetable .14
Bibliography .15
© ISO 2020 – All rights reserved iii
---------------------- Page: 3 ----------------------
ISO 11050:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4,
Cereals and pulses.
This second edition cancels and replaces the first edition (ISO 11050:1993), which has been technically
revised. The main changes compared with the previous edition are as follows:
— the Scope has been widened;
— the protocol has been improved to make it easier;
— the figures have been updated.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved
---------------------- Page: 4 ----------------------
INTERNATIONAL STANDARD ISO 11050:2020(E)
Wheat flour and durum wheat semolina — Determination
of impurities of animal origin
1 Scope
This document specifies a method for determining the content of impurities of animal origin in wheat
flours, with or without additives and having an ash yield not exceeding a mass fraction of 0,75 %, and in
durum wheat semolinas.
This method permits the separation and quantification of contamination of animal origin, such as insects
at all stages of their development and their fragments, rodent hairs and their fragments, and mites.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
impurity of animal origin
matter of animal origin [larvae, nymphs or adults of insects (3.10) and their fragments, rodent hairs
and their fragments, mites (3.3)] separated from the product under the conditions specified in this
document
3.2
abdomen
rear part of the body of an insect (3.10), excluding the head and thorax, commonly with eight or more
segments when complete
3.3
mite
very small arthropod belonging to the class Arachnida, often living in large numbers
3.4
appendice
distinctly differentiated prolongation of the body of an arthropod
EXAMPLE Legs, wings, antennae, urogomphi (3.16).
3.5
cephalic capsule
head capsule
sclerous part of an exuvia that once contained the head of a larva
© ISO 2020 – All rights reserved 1
---------------------- Page: 5 ----------------------
ISO 11050:2020(E)
3.6
caterpillar
larvae of Lepidoptera spp.
Note 1 to entry: Butterfly or moth is the adult stage and chrysalis is the pupal stage.
3.7
scale
bristle (3.13) that has evolved into a flat structure resembling a fish scale and that covers the parts of
the body of certain insects (3.10), in particular the wings of Lepidoptera spp.
3.8
wing case
elytron
hardened front wing of Coleoptera spp.
Note 1 to entry: It is used as a fixed wing in flight and as protective cover for the membranous hind wing.
3.9
false leg
proleg
fleshy extension of the lower part of the abdomen (3.2) of some larvae, sometimes with a crown of fine
hooks (crochets) of chitin
Note 1 to entry: These help with attachment to the substrate, and in movement. Lepidopterous larvae have at
least two pairs of false legs, towards the rear of the body.
3.10
insect
class of animals within the phylum Arthropoda, some of which are recognized pests of stored foodstuffs
3.11
mandible
toughened (sclerotized) mouthpart of insects (3.10)
Note 1 to entry: It is used for the dilaceration or grinding of food.
3.12
pericarp
external envelope of seeds that forms the bran after the grain has been crushed and the flour separated
3.13
bristle
fine but stiff hair of any length present on the cuticle of insects (3.10)
Note 1 to entry: Sensory hairs are called “setae”.
3.14
stage
state of development of an insect (3.10) or mite (3.3)
EXAMPLE Egg, larva, nymph, pupa, adult.
3.15
juvenile stage
pre-adult stage (3.14) of insects (3.10)
EXAMPLE Egg, larva, nymph, pupa.
Note 1 to entry: This term is most often applied to the active stages of larva and nymph.
2 © ISO 2020 – All rights reserved
---------------------- Page: 6 ----------------------
ISO 11050:2020(E)
3.16
urogomphi
pointed extensions of the cuticle of the final abdominal segment of some insect larvae
Note 1 to entry: They are common, and sometimes diagnostic, features of many Coleoptera spp.
Note 2 to entry: The abdominal extensions of a cockroach are called “cerci”.
4 Principle
Hydrolysis of a test portion with a solution of hydrochloric acid at boiling point. Concentration of the
insoluble particles (impurities other than those of animal origin may be present) at a water/oil interface.
Separation by filtration on a filter paper, microscopic examination and counting under reflected light of
the impurities of animal origin.
5 Reagents
Only use reagents of a recognized analytical grade and demineralized water or water of equivalent purity.
5.1 Ethanol, a volume fraction of 95 %.
5.2 Ethanol solution, a volume fraction of 50 %.
5.3 Ethanol/glycerol, 1 + 1 mixture by volume.
5.4 Hydrochloric acid solution, concentrated at 35 % to 37 %.
5.5 Paraffin oil (known as “Vaseline oil”), fluid, having a viscosity not exceeding 60 mPa·s at 20 °C.
5.6 Liquid detergent, non-foaming.
1)
EXAMPLE Extran, Biodeck 4, Decon 90.
5.7 Liquid detergent, a volume fraction of 1 % aqueous solution of the detergent (5.6) in a
washing bottle.
5.8 Dearomatized white spirit (possible use).
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
1) These are examples of suitable products available commercially. This information is given for the convenience
of users of this document and does not constitute an endorsement by ISO of these products.
© ISO 2020 – All rights reserved 3
---------------------- Page: 7 ----------------------
ISO 11050:2020(E)
6.1 Separating funnels, conical, of 1 000 ml capacity, fitted with a non-lubricated
polytetrafluoroethylene (PTFE) tap. See the recommended set-up shown in Figure 1.
Key
1 light hydrocarbon phase 3 “heavy” aqueous phase
2 support ring 4 PTFE tap
Figure 1 — Separation apparatus
2)
6.2 Tall-form beaker, of at least 800 ml capacity, fitted with a watch glass made of Pyrex® or PFTE
and of appropriate dimensions to serve as a lid.
6.3 Crystallizing dish or pan, of at least 5 l capacity and of a height slightly less than that of the beaker
(6.2), suitable for use as a cooling bath.
6.4 Graduated cylinders, of 25 ml, 50 ml and 500 ml capacity.
6.5 Washing bottles, with a flexible tube.
2) Pyrex® is the trademark of a product registered by Corning Inc. This information is given for the convenience of
users of this document and does not constitute an endorsement by ISO of the product named. Equivalent products
may be used if they can be shown to lead to the same results.
4 © ISO 2020 – All rights reserved
---------------------- Page: 8 ----------------------
ISO 11050:2020(E)
3)
6.6 Filter paper , ash free, with rapid filtration characteristics, of 90 mm diameter, corresponding
to that of the filtration unit (6.7), on which fine parallel lines are drawn, spaced 5 mm apart, using a
lead pencil.
6.7 Filtration unit, of the Büchner funnel type, removable, suitable for accommodating the filter paper
(6.6), and fitted with a conical adapter bung for connection to the filtration flask (6.15).
6.8 Analytical balance, for precision weighing to within 0,1 g.
6.9 Optical microscope or stereoscopic microscope, known as a “binocular magnifying glass”, capable
of producing magnifications close to ×25 and ×50, of very high optical quality, used in conjunction with:
a) eyepieces, producing a magnification of ×15 or
...
NORME ISO
INTERNATIONALE 11050
Deuxième édition
2020-04
Farines de blé tendre et semoules
de blé dur — Détermination des
impuretés d'origine animale
Wheat flour and durum wheat semolina — Determination of
impurities of animal origin
Numéro de référence
ISO 11050:2020(F)
©
ISO 2020
---------------------- Page: 1 ----------------------
ISO 11050:2020(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2020
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: +41 22 749 01 11
Fax: +41 22 749 09 47
E-mail: copyright@iso.org
Web: www.iso.org
Publié en Suisse
ii © ISO 2020 – Tous droits réservés
---------------------- Page: 2 ----------------------
ISO 11050:2020(F)
Sommaire Page
Avant-propos .iv
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe . 3
5 Réactifs . 3
6 Appareillage . 3
7 Échantillonnage . 5
8 Mode opératoire. 6
8.1 Prise d’essai . 6
8.2 Hydrolyse . 6
8.3 Séparation des impuretés . 6
8.4 Filtration . 7
8.5 Examen au microscope . 7
8.6 Nombre de déterminations . 8
9 Expression des résultats. 8
10 Répétabilité . 8
11 Rapport d’essai . 8
Annexe A (informative) Caractéristiques des fragments retrouvés sur les filtres .9
Annexe B (informative) Rapport d’essai — Détermination des impuretés animales selon le
présent document .11
Annexe C (informative) Schéma du mode opératoire .12
Annexe D (informative) Chronologie des opérations et bilan horaire .14
Bibliographie .15
© ISO 2020 – Tous droits réservés iii
---------------------- Page: 3 ----------------------
ISO 11050:2020(F)
Avant-propos
L’ISO (Organisation internationale de normalisation) est une fédération mondiale d’organismes
nationaux de normalisation (comités membres de l’ISO). L’élaboration des Normes internationales est
en général confiée aux comités techniques de l’ISO. Chaque comité membre intéressé par une étude
a le droit de faire partie du comité technique créé à cet effet. Les organisations internationales,
gouvernementales et non gouvernementales, en liaison avec l’ISO participent également aux travaux.
L’ISO collabore étroitement avec la Commission électrotechnique internationale (IEC) en ce qui
concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier, de prendre note des différents
critères d’approbation requis pour les différents types de documents ISO. Le présent document a été
rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir www
.iso .org/ directives).
L’attention est attirée sur le fait que certains des éléments du présent document peuvent faire l’objet de
droits de propriété intellectuelle ou de droits analogues. L’ISO ne saurait être tenue pour responsable
de ne pas avoir identifié de tels droits de propriété et averti de leur existence. Les détails concernant
les références aux droits de propriété intellectuelle ou autres droits analogues identifiés lors de
l’élaboration du document sont indiqués dans l’Introduction et/ou dans la liste des déclarations de
brevets reçues par l’ISO (voir www .iso .org/ brevets).
Les appellations commerciales éventuellement mentionnées dans le présent document sont données
pour information, par souci de commodité, à l’intention des utilisateurs et ne sauraient constituer un
engagement.
Pour une explication de la nature volontaire des normes, la signification des termes et expressions
spécifiques de l’ISO liés à l’évaluation de la conformité, ou pour toute information au sujet de l’adhésion
de l’ISO aux principes de l’Organisation mondiale du commerce (OMC) concernant les obstacles
techniques au commerce (OTC), voir www .iso .org/ avant -propos.
Le présent document a été élaboré par le comité technique ISO/TC 34, Produits alimentaires, sous-
comité SC 4, Céréales et légumineuses.
Cette deuxième édition annule et remplace la première édition (ISO 11050:1993) qui a fait l’objet d’une
révision technique. Les principales modifications par rapport à l’édition précédente sont les suivantes:
— le domaine d’application a été élargi;
— le protocole a été amélioré pour le rendre plus simple;
— les chiffres ont été actualisés.
Il convient que l’utilisateur adresse tout retour d’information ou toute question concernant le présent
document à l’organisme national de normalisation de son pays. Une liste exhaustive desdits organismes
se trouve à l’adresse www .iso .org/ fr/ members .html.
iv © ISO 2020 – Tous droits réservés
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NORME INTERNATIONALE ISO 11050:2020(F)
Farines de blé tendre et semoules de blé dur —
Détermination des impuretés d'origine animale
1 Domaine d’application
Le présent document spécifie une méthode de détermination des impuretés d’origine animale dans
les farines de blé tendre, avec ou sans additif, ayant un taux de cendres inférieur ou égal à 0,75 % en
fraction massique, et dans les semoules de blé dur.
Cette méthode permet de séparer et de dénombrer les souillures d’origine animale, telles que les
insectes à tous les stades de leur développement et leurs fragments, les poils de rongeurs et leurs
fragments, ainsi que les acariens.
2 Références normatives
Le présent document ne contient aucune référence normative.
3 Termes et définitions
Pour les besoins du présent document, les termes et définitions suivants s’appliquent.
L’ISO et l’IEC tiennent à jour des bases de données terminologiques destinées à être utilisées en
normalisation, consultables aux adresses suivantes:
— ISO Online browsing platform: disponible à l’adresse https:// www .iso .org/ obp
— IEC Electropedia: disponible à l’adresse http:// www .electropedia .org/
3.1
impureté d’origine animale
matière d’origine animale [larves, nymphes ou adultes d’insectes (3.10) et leurs fragments, poils de
rongeurs et leurs fragments, acariens (3.3)] séparée du produit dans les conditions spécifiées dans le
présent document
3.2
abdomen
partie postérieure du corps d’un insecte (3.10), sans tête ni thorax, comportant huit segments ou plus
lorsqu’il est entier
3.3
acarien
arthropode de très petite taille, de la classe des Arachnida, vivant souvent en colonies
3.4
appendice
prolongement nettement différencié du corps d’un arthropode
EXEMPLE Pattes, ailes, antennes et urogomphes (3.16).
3.5
capsule céphalique
capsule de tête
partie sclérotinisée d’une exuvie contenant la tête de la larve qui a mué
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ISO 11050:2020(F)
3.6
chenille
larve des Lepidoptera spp.
Note 1 à l'article: Le papillon constitue l’adulte et la nymphe s’appelle chrysalide.
3.7
écaille
soie (3.13) transformée en élément plat ressemblant à une écaille de poisson et qui recouvre le corps de
certains insectes (3.10), en particulier les ailes des Lepidoptera spp.
3.8
élytre
aile antérieure indurée des Coleoptera spp.
Note 1 à l'article: Est utilisé comme aile fixe durant le vol et comme étui protecteur des ailes postérieures
membraneuses.
3.9
fausse patte
extension charnue de la partie inférieure de l’abdomen (3.2) de certaines larves, présentant parfois une
couronne de fins crochets en chitine
Note 1 à l'article: Ceux-ci aident à la fixation au substrat et au déplacement. Les larves de lépidoptères ont au
moins deux paires de fausses pattes placées à la partie postérieure du corps.
3.10
insecte
classe d’animaux de l’embranchement des arthropodes, dont certains sont considérés comme nuisibles
pour les denrées entreposées
3.11
mandibule
pièce buccale (sclérotinisée) des insectes (3.10)
Note 1 à l'article: Sert à la dilacération ou au broyage des aliments.
3.12
péricarpe
enveloppe externe du grain qui donne le son après écrasement du grain et séparation de la farine
3.13
soie
poil fin mais raide, plus ou moins long, présent sur la cuticule des insectes (3.10)
Note 1 à l'article: Les poils sensoriels sont appelés «setae».
3.14
stade
état de développement d’un insecte (3.10) ou d’un acarien (3.3)
EXEMPLE Œuf, larve, nymphe, chrysalide, adulte.
3.15
stade juvénile
stade (3.14) du développement des insectes (3.10) avant qu’ils ne soient adultes
EXEMPLE Œuf, larve, nymphe, chrysalide.
Note 1 à l'article: Ce terme est utilisé le plus souvent pour désigner les stades actifs de la larve et de la nymphe.
2 © ISO 2020 – Tous droits réservés
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ISO 11050:2020(F)
3.16
urogomphes
saillies en forme de pointes de la cuticule du dernier segment abdominal des larves de certains insectes
Note 1 à l'article: Ces éléments communs à de nombreuses espèces de Coleoptera spp. sont parfois distinctifs.
Note 2 à l'article: Les extensions abdominales des cafards sont appelées «cerci».
4 Principe
Hydrolyse d’une prise d’essai par une solution d’acide chlorhydrique à ébullition. Concentration des
particules insolubles (il peut y avoir des impuretés autres que celles d’origine animale) à une interface
eau/huile. Séparation par filtration sur papier filtre, examen au microscope et dénombrement en
lumière réfléchie des impuretés d’origine animale.
5 Réactifs
Utiliser uniquement des réactifs de qualité analytique reconnue et de l’eau déminéralisée ou de pureté
équivalente.
5.1 Éthanol, d’une fraction volumique de 95 %.
5.2 Solution d’éthanol, d’une fraction volumique de 50 %.
5.3 Éthanol/glycérol, mélange 1 + 1 en volume.
5.4 Solution d’acide chlorhydrique, concentrée de 35 % à 37 %.
5.5 Huile de paraffine (dite «huile de vaseline»), fluide, de viscosité inférieure ou égale à 60 mPa·s
à 20 °C.
5.6 Détergent liquide, non moussant.
1)
EXEMPLE Extran, Biodeck 4, Decon 90 .
5.7 Détergent liquide, solution aqueuse de 1 % en fraction volumique de détergent (5.6) dans une
pissette.
5.8 White spirit désaromatisé (utilisation possible).
6 Appareillage
Matériel courant de laboratoire et, en particulier, ce qui suit.
1) Ce sont des exemples de produits appropriés disponibles sur le marché. Ces informations sont données par
souci de commodité à l’intention des utilisateurs du présent document et ne sauraient constituer un engagement de
l’ISO à l’égard de ces produits.
© ISO 2020 – Tous droits réservés 3
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ISO 11050:2020(F)
6.1 Ampoules à décanter, de forme conique, d’une contenance de 1 000 ml, munies d’un robinet en
polytétrafluoroéthylène (PTFE) non graissé. Voir le système conseillé à la Figure 1.
Légende
1 phase légère d’hydrocarbure 3 phase aqueuse «lourde»
2 cercle support 4 robinet en PTFE
Figure 1 — Dispositif de décantation
6.2 Bécher de forme haute, d’une contenance d’au moins 800 ml, muni d’un verre de montre en
2)
Pyrex® ou en PFTE de dimensions appropriées pour servir de couvercle.
6.3 Cristallisoir ou cuvette, d’une contenance d’au moins 5 l et de hauteur légèrement inférieure à
celle du bécher (6.2), servant de bain de refroidissement.
6.4 Éprouvettes graduées, d’une contenance de 25 ml, 50 ml et 500 ml.
6.5 Pissettes, à embout flexible.
2) Pyrex® est la marque d’un produit distribué par Corning Inc. Ces informations sont données par souci de
commodité à l’intention des utilisateurs du présent document et ne sauraient constituer un engagement de l’ISO à
l’égard de ce produit. Des produits équivalents peuvent être utilisés s’il est démontré qu’ils conduisent aux mêmes
résultats.
4 © ISO 2020 – Tous droits réservés
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ISO 11050:2020(F)
3)
6.6 Papier filtre , sans cendres, à filtration rapide, de 90 mm de diamètre, correspondant à celui de
l’unité de filtration (6.7), sur lequel on tracera de fines rayures parallèles espacées de 5 mm à l’aide d’un
crayon à mine de plomb.
6.7 Unité de filtration, type entonnoir de Büchner, démontable, destinée à recevoir le papier filtre
(6.6), avec un bouchon conique d’adaptation sur la fiole à filtrer (6.15).
6.8 Balance analytique, précise à 0,1 g près.
6.9 Microscope optique ou microscope stéréoscopique, dit «loupe binoculaire», à grossissements
voisins de × 25 et × 50, de très bonne qualité optique et complété par:
a) des oculaires de grossissement de × 15 ou × 20 permettant un grossissement total maxi
...
SLOVENSKI STANDARD
oSIST ISO 11050:2020
01-november-2020
Pšenična moka in pšenični zdrob durum - Določevanje nečistoč živalskega izvora
Wheat flour and durum wheat semolina -- Determination of impurities of animal origin
Farines de blé tendre et semoules de blé dur -- Détermination des impuretés d'origine
animale
Ta slovenski standard je istoveten z: ISO 11050:2020
ICS:
67.060 Žita, stročnice in proizvodi iz Cereals, pulses and derived
njih products
oSIST ISO 11050:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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oSIST ISO 11050:2020
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oSIST ISO 11050:2020
INTERNATIONAL ISO
STANDARD 11050
Second edition
2020-04
Wheat flour and durum wheat
semolina — Determination of
impurities of animal origin
Farines de blé tendre et semoules de blé dur — Détermination des
impuretés d'origine animale
Reference number
ISO 11050:2020(E)
©
ISO 2020
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oSIST ISO 11050:2020
ISO 11050:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
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oSIST ISO 11050:2020
ISO 11050:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents . 3
6 Apparatus . 3
7 Sampling . 5
8 Procedure. 6
8.1 Test portion . 6
8.2 Hydrolysis . 6
8.3 Separation of impurities . 6
8.4 Filtration . 7
8.5 Microscopic examination . 7
8.6 Number of determinations . 8
9 Expression of results . 8
10 Repeatability . 8
11 Test report . 8
Annex A (informative) Characteristics of fragments found on the filters . 9
Annex B (informative) Test report — Determination of animal impurities in accordance
with this document .11
Annex C (informative) Diagram of the procedure .12
Annex D (informative) Chronology of operations and timetable .14
Bibliography .15
© ISO 2020 – All rights reserved iii
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oSIST ISO 11050:2020
ISO 11050:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4,
Cereals and pulses.
This second edition cancels and replaces the first edition (ISO 11050:1993), which has been technically
revised. The main changes compared with the previous edition are as follows:
— the Scope has been widened;
— the protocol has been improved to make it easier;
— the figures have been updated.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved
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oSIST ISO 11050:2020
INTERNATIONAL STANDARD ISO 11050:2020(E)
Wheat flour and durum wheat semolina — Determination
of impurities of animal origin
1 Scope
This document specifies a method for determining the content of impurities of animal origin in wheat
flours, with or without additives and having an ash yield not exceeding a mass fraction of 0,75 %, and in
durum wheat semolinas.
This method permits the separation and quantification of contamination of animal origin, such as insects
at all stages of their development and their fragments, rodent hairs and their fragments, and mites.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
impurity of animal origin
matter of animal origin [larvae, nymphs or adults of insects (3.10) and their fragments, rodent hairs
and their fragments, mites (3.3)] separated from the product under the conditions specified in this
document
3.2
abdomen
rear part of the body of an insect (3.10), excluding the head and thorax, commonly with eight or more
segments when complete
3.3
mite
very small arthropod belonging to the class Arachnida, often living in large numbers
3.4
appendice
distinctly differentiated prolongation of the body of an arthropod
EXAMPLE Legs, wings, antennae, urogomphi (3.16).
3.5
cephalic capsule
head capsule
sclerous part of an exuvia that once contained the head of a larva
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oSIST ISO 11050:2020
ISO 11050:2020(E)
3.6
caterpillar
larvae of Lepidoptera spp.
Note 1 to entry: Butterfly or moth is the adult stage and chrysalis is the pupal stage.
3.7
scale
bristle (3.13) that has evolved into a flat structure resembling a fish scale and that covers the parts of
the body of certain insects (3.10), in particular the wings of Lepidoptera spp.
3.8
wing case
elytron
hardened front wing of Coleoptera spp.
Note 1 to entry: It is used as a fixed wing in flight and as protective cover for the membranous hind wing.
3.9
false leg
proleg
fleshy extension of the lower part of the abdomen (3.2) of some larvae, sometimes with a crown of fine
hooks (crochets) of chitin
Note 1 to entry: These help with attachment to the substrate, and in movement. Lepidopterous larvae have at
least two pairs of false legs, towards the rear of the body.
3.10
insect
class of animals within the phylum Arthropoda, some of which are recognized pests of stored foodstuffs
3.11
mandible
toughened (sclerotized) mouthpart of insects (3.10)
Note 1 to entry: It is used for the dilaceration or grinding of food.
3.12
pericarp
external envelope of seeds that forms the bran after the grain has been crushed and the flour separated
3.13
bristle
fine but stiff hair of any length present on the cuticle of insects (3.10)
Note 1 to entry: Sensory hairs are called “setae”.
3.14
stage
state of development of an insect (3.10) or mite (3.3)
EXAMPLE Egg, larva, nymph, pupa, adult.
3.15
juvenile stage
pre-adult stage (3.14) of insects (3.10)
EXAMPLE Egg, larva, nymph, pupa.
Note 1 to entry: This term is most often applied to the active stages of larva and nymph.
2 © ISO 2020 – All rights reserved
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oSIST ISO 11050:2020
ISO 11050:2020(E)
3.16
urogomphi
pointed extensions of the cuticle of the final abdominal segment of some insect larvae
Note 1 to entry: They are common, and sometimes diagnostic, features of many Coleoptera spp.
Note 2 to entry: The abdominal extensions of a cockroach are called “cerci”.
4 Principle
Hydrolysis of a test portion with a solution of hydrochloric acid at boiling point. Concentration of the
insoluble particles (impurities other than those of animal origin may be present) at a water/oil interface.
Separation by filtration on a filter paper, microscopic examination and counting under reflected light of
the impurities of animal origin.
5 Reagents
Only use reagents of a recognized analytical grade and demineralized water or water of equivalent purity.
5.1 Ethanol, a volume fraction of 95 %.
5.2 Ethanol solution, a volume fraction of 50 %.
5.3 Ethanol/glycerol, 1 + 1 mixture by volume.
5.4 Hydrochloric acid solution, concentrated at 35 % to 37 %.
5.5 Paraffin oil (known as “Vaseline oil”), fluid, having a viscosity not exceeding 60 mPa·s at 20 °C.
5.6 Liquid detergent, non-foaming.
1)
EXAMPLE Extran, Biodeck 4, Decon 90.
5.7 Liquid detergent, a volume fraction of 1 % aqueous solution of the detergent (5.6) in a
washing bottle.
5.8 Dearomatized white spirit (possible use).
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
1) These are examples of suitable products available commercially. This information is given for the convenience
of users of this document and does not constitute an endorsement by ISO of these products.
© ISO 2020 – All rights reserved 3
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oSIST ISO 11050:2020
ISO 11050:2020(E)
6.1 Separating funnels, conical, of 1 000 ml capacity, fitted with a non-lubricated
polytetrafluoroethylene (PTFE) tap. See the recommended set-up shown in Figure 1.
Key
1 light hydrocarbon phase 3 “heavy” aqueous phase
2 support ring 4 PTFE tap
Figure 1 — Separation apparatus
2)
6.2 Tall-form beaker, of at least 800 ml capacity, fitted with a watch glass made of Pyrex® or PFTE
and of appropriate dimensions to serve as a lid.
6.3 Crystallizing dish or pan, of at least 5 l capacity and of a height slightly less than that of the beaker
(6.2), suitable for use as a cooling bath.
6.4 Graduated cylinders, of 25 ml, 50 ml and 500 ml capacity.
6.5 Washing bottles, with a flexible tube.
2) Pyrex® is the trademark of a product registered by Corning Inc. This information is given for the convenience of
users of this document and does not constitute an endorsement by ISO of the product named. Equivalent products
may be used if they can be shown to lead to the same results.
4 © ISO 2020 – All rights reserved
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oSIST ISO 11050:2020
ISO 11050:2020(E)
3)
6.6 Filter paper , ash free, with rapid filtration characteristics, of 90 mm diameter, corresponding
to that of the filtration unit (6.7), on which fine parallel lines are drawn, spaced 5 mm apart, using a
lead pencil.
6.7 Filtration unit, of the Büchner funnel type, removable, suitable for accommodating th
...
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