ISO/TS 23016-1:2019

Fine bubble technology -- Agricultural applications

This document specifies a test method for evaluating the effect of fine bubble water on the growth promotion of hydroponically grown lettuce by estimating the incremental gain in mass of the stems and leaves over a specified growth period.

Technologie des fines bulles -- Applications agricoles

General Information

• Status: Published
• Publication Date: 19-May-2019
• ICS: 07.030 - Physics. Chemistry
• ICS: 65.020.20 - Plant growing
• Technical Committee: ISO/TC 281 - Fine bubble technology
• Drafting Committee: ISO/TC 281/WG 3 - Applications of fine bubble technology
• Current Stage: 6060 - International Standard published
• Start Date: 24-Apr-2019
• Completion Date: 20-May-2019

Standards Content (sample)

TECHNICAL ISO/TS
SPECIFICATION 23016-1
First edition
2019-05
Fine bubble technology — Agricultural
applications —
Part 1:
Test method for evaluating the
growth promotion of hydroponically
grown lettuce
Reference number
ISO/TS 23016-1:2019(E)
ISO 2019
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ISO/TS 23016-1:2019(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2019

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ii © ISO 2019 – All rights reserved
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ISO/TS 23016-1:2019(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

Introduction ..................................................................................................................................................................................................................................v

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Test method for growth promotion performance on lettuce ................................................................................. 2

4.1 Conditions of test environment ................................................................................................................................................ 2

4.2 Test system and related apparatus ........................................................................................................................................ 2

4.3 Materials for test ................................................................................................................................................................................... 4

4.4 Preparation for test ............................................................................................................................................................................. 4

4.4.1 Confirmation of the working conditions of the fine bubble line system ......................... 4

4.4.2 Hydroponic bed system ............................................................................................................................................. 4

4.4.3 Operator(s) and inspector(s) ................................................................................................................................ 5

4.4.4 Operation manual ........................................................................................................................................................... 5

4.5 Preliminary test for confirmation of reproducibility ............................................................................................. 5

4.5.1 General...................................................................................................................................................................................... 5

4.5.2 Confirmation of reproducibility .......................................................................................................................... 5

4.5.3 Actions when there is a statistically significant difference ......................................................... 5

4.6 Test procedure ......................................................................................................................................................................................... 5

4.6.1 Preparation of lettuce seedling ........................................................................................................................... 5

4.6.2 Growth system ................................................................................................................................................................... 5

4.6.3 Sampling.................................................................................................................................................................................. 5

4.6.4 Records ..................................................................................................................................................................................... 6

4.6.5 Number of tests ................................................................................................................................................................. 6

4.7 Calculation of degree of growth promotion ................................................................................................................... 7

4.8 Test report ................................................................................................................................................................................................... 7

Annex A (informative) Example of test results for lettuce growth promotion performance .....................8

Annex B (informative) Example of recording format for measurement figures of

environmental parameters ....................................................................................................................................................................11

Bibliography .............................................................................................................................................................................................................................12

© ISO 2019 – All rights reserved iii
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ISO/TS 23016-1:2019(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso

.org/iso/foreword .html.

This document was prepared by Technical Committee ISO/TC 281, Fine bubble technology.

A list of all parts in the ISO 23016 series can be found on the ISO website.

Any feedback or questions on this document should be directed to the user’s national standards body. A

complete listing of these bodies can be found at www .iso .org/members .html
iv © ISO 2019 – All rights reserved
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ISO/TS 23016-1:2019(E)
Introduction

The recent progress in the application of fine bubble technology exhibits successes in the various

technical fields such as environmental technology in water and washing and cleaning technology for

mechanical engineering. The applications for agro- and aqua- farming and food industrial field also

draw high interests of markets in view of fine bubble enabled performance in enhancing growth of

agro- and aqua- products, improving their quality, saving resources for farming and ensuring safety

of the food products. Various industries engaged in such products are introducing the fine bubbles to

their farming field by applying fine bubble generating systems, eventually creating new market for the

generating systems.

However, since technology transfer from fine bubble technology to technology fields of agro- and aqua-

farming and food industries is not well supported by common understanding of the fine bubbles or their

generating technology, results of evaluation on fine bubble enhanced performance cannot be accepted

commonly by both generating system suppliers and its users at the transaction scene. Furthermore,

a variety of agro- and aqua- farming products makes it difficult to adopt a systematic approach for

selection and application of generating systems.

The performance evaluation based on objective evidence resulting from standardized procedures

is intended to bridge the two technologies and facilitate diverse fields of applications for fine bubble

technology in the global market. In order to accelerate sound global market formation, development of

test procedures is urgently demanded by both technology stakeholders.

This document is intended to meet these needs by specifying the test procedure to be applied to the

generating system for agro- and aqua- farming and food industries uses. The evaluation is made by

applying fine bubble water generated by the object system to lettuce and by measuring its growth. The

product, lettuce, is globally accepted and the yielded test data represents the performance of the tested

system over other products in such major product family as, for example, leaf vegetable. The growth

process of lettuce is much simpler than other vegetables making the measuring process much easier in

the test procedure. The specified test conditions, namely the environment for growth, are also easy to

be controlled allowing many testing plants globally available. The parameter measured is the change in

the harvested mass of lettuces with application of fine bubbles compared to that without application in

a specified period of growth.

Since the performance in terms of parameters is improving rapidly as the technology evolves, the

quantitative criteria for the testing are not specified in this document.
© ISO 2019 – All rights reserved v
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TECHNICAL SPECIFICATION ISO/TS 23016-1:2019(E)
Fine bubble technology — Agricultural applications —
Part 1:
Test method for evaluating the growth promotion of
hydroponically grown lettuce
1 Scope

This document specifies a test method for evaluating the effect of fine bubble water on the growth

promotion of hydroponically grown lettuce by estimating the incremental gain in mass of the stems

and leaves over a specified growth period.
2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 20480-1, Fine bubble technology — General principles for usage and measurement of fine bubbles —

Part 1: Terminology

ISO 20480-2, Fine bubble technology — General principles for usage and measurement of fine bubbles —

Part 2: Categorization of the attributes of fine bubbles
3 Terms and definitions

For the purposes of this document, the terms and definitions given in ISO 20480-1 and ISO 20480-2 and

the following apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— ISO Online browsing platform: available at https: //www .iso .org/obp
— IEC Electropedia: available at http: //www .electropedia .org/
3.1
plant factory

facilities which allow systematic growth and production of plants where the internal environment

conditions, e.g. temperature, carbon dioxide, and liquid fertilizer are controlled

3.2
fine bubble generating system

system which mechanically generates fine bubbles (bubbles less than 100 μm in diameter) using

water and air
3.3
fine bubble water
water including fine bubbles and used in plant factories (3.1)
3.4
raw water

tap water or water with equivalent quality level provided to produce fine bubble water (3.3) and used as

the control water for the reference
© ISO 2019 – All rights reserved 1
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ISO/TS 23016-1:2019(E)
3.5
fine bubble section
area to grow plants using fine bubble water (3.3)
Note 1 to entry: For the purpose of this document, plants grown are lettuces.
3.6
control section

area to grow plants using raw water (3.4) as a reference for fine bubble section (3.6)

Note 1 to entry: For the purpose of this document, plants grown are lettuces.
3.7
liquid fertilizer
liquid used as nutrient to grow plants
3.8
culture solution

solution containing liquid fertilizer (3.7) supplied to hydroponic cultivation system

3.9
photosynthetic photon flux density
PPFD

number of photons per unit of time and area, which are contained in 400 nm – 700 nm of wave length

needed for photosynthesis
4 Test method for growth promotion performance on lettuce
4.1 Conditions of test environment

4.1.1 Water supply. Sufficient water supply shall be provided to circulate the water in the piping of the

hydroponic bed and the tank.

4.1.2 Air quality. There shall be no significantly abnormal values that would have impact on lettuce

growth, compared with normal air composition.
4.1.3 Operation of growth promotion
...