ISO/TS 23016-1:2019
(Main)Fine bubble technology — Agricultural applications — Part 1: Test method for evaluating the growth promotion of hydroponically grown lettuce
Fine bubble technology — Agricultural applications — Part 1: Test method for evaluating the growth promotion of hydroponically grown lettuce
This document specifies a test method for evaluating the effect of fine bubble water on the growth promotion of hydroponically grown lettuce by estimating the incremental gain in mass of the stems and leaves over a specified growth period.
Technologie des fines bulles — Applications agricoles — Partie 1: Titre manque
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TECHNICAL ISO/TS
SPECIFICATION 23016-1
First edition
2019-05
Fine bubble technology — Agricultural
applications —
Part 1:
Test method for evaluating the
growth promotion of hydroponically
grown lettuce
Reference number
©
ISO 2019
© ISO 2019
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ii © ISO 2019 – All rights reserved
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Test method for growth promotion performance on lettuce . 2
4.1 Conditions of test environment . 2
4.2 Test system and related apparatus . 2
4.3 Materials for test . 4
4.4 Preparation for test . 4
4.4.1 Confirmation of the working conditions of the fine bubble line system . 4
4.4.2 Hydroponic bed system . 4
4.4.3 Operator(s) and inspector(s) . 5
4.4.4 Operation manual . 5
4.5 Preliminary test for confirmation of reproducibility . 5
4.5.1 General. 5
4.5.2 Confirmation of reproducibility . 5
4.5.3 Actions when there is a statistically significant difference . 5
4.6 Test procedure . 5
4.6.1 Preparation of lettuce seedling . 5
4.6.2 Growth system . 5
4.6.3 Sampling. 5
4.6.4 Records . 6
4.6.5 Number of tests . 6
4.7 Calculation of degree of growth promotion . 7
4.8 Test report . 7
Annex A (informative) Example of test results for lettuce growth promotion performance .8
Annex B (informative) Example of recording format for measurement figures of
environmental parameters .11
Bibliography .12
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
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expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 281, Fine bubble technology.
A list of all parts in the ISO 23016 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html
iv © ISO 2019 – All rights reserved
Introduction
The recent progress in the application of fine bubble technology exhibits successes in the various
technical fields such as environmental technology in water and washing and cleaning technology for
mechanical engineering. The applications for agro- and aqua- farming and food industrial field also
draw high interests of markets in view of fine bubble enabled performance in enhancing growth of
agro- and aqua- products, improving their quality, saving resources for farming and ensuring safety
of the food products. Various industries engaged in such products are introducing the fine bubbles to
their farming field by applying fine bubble generating systems, eventually creating new market for the
generating systems.
However, since technology transfer from fine bubble technology to technology fields of agro- and aqua-
farming and food industries is not well supported by common understanding of the fine bubbles or their
generating technology, results of evaluation on fine bubble enhanced performance cannot be accepted
commonly by both generating system suppliers and its users at the transaction scene. Furthermore,
a variety of agro- and aqua- farming products makes it difficult to adopt a systematic approach for
selection and application of generating systems.
The performance evaluation based on objective evidence resulting from standardized procedures
is intended to bridge the two technologies and facilitate diverse fields of applications for fine bubble
technology in the global market. In order to accelerate sound global market formation, development of
test procedures is urgently demanded by both technology stakeholders.
This document is intended to meet these needs by specifying the test procedure to be applied to the
generating system for agro- and aqua- farming and food industries uses. The evaluation is made by
applying fine bubble water generated by the object system to lettuce and by measuring its growth. The
product, lettuce, is globally accepted and the yielded test data represents the performance of the tested
system over other products in such major product family as, for example, leaf vegetable. The growth
process of lettuce is much simpler than other vegetables making the measuring process much easier in
the test procedure. The specified test conditions, namely the environment for growth, are also easy to
be controlled allowing many testing plants globally available. The parameter measured is the change in
the harvested mass of lettuces with application of fine bubbles compared to that without application in
a specified period of growth.
Since the performance in terms of parameters is improving rapidly as the technology evolves, the
quantitative criteria for the testing are not specified in this document.
TECHNICAL SPECIFICATION ISO/TS 23016-1:2019(E)
Fine bubble technology — Agricultural applications —
Part 1:
Test method for evaluating the growth promotion of
hydroponically grown lettuce
1 Scope
This document specifies a test method for evaluating the effect of fine bubble water on the growth
promotion of hydroponically grown lettuce by estimating the incremental gain in mass of the stems
and leaves over a specified growth period.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 20480-1, Fine bubble technology — General principles for usage and measurement of fine bubbles —
Part 1: Terminology
ISO 20480-2, Fine bubble technology — General principles for usage and measurement of fine bubbles —
Part 2: Categorization of the attributes of fine bubbles
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 20480-1 and ISO 20480-2 and
the following apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https: //www .iso .org/obp
— IEC Electropedia: available at http: //www .electropedia .org/
3.1
plant factory
facilities which allow systematic growth and production of plants where the internal environment
conditions, e.g. temperature, carbon dioxide, and liquid fertilizer are controlled
3.2
fine bubble generating system
system which mechanically generates fine bubbles (bubbles less than 100 μm in diameter) using
water and air
3.3
fine bubble water
water including fine bubbles and used in plant factories (3.1)
3.4
raw water
tap water or water with equivalent quality level provided to produce fine bubble water (3.3) and used as
the control water for the reference
3.5
fine bubble section
area to grow plants using fine bubble water (3.3)
Note 1 to entry: For the purpose of this document, plants grown are lettuces.
3.6
control section
area to grow plants using raw water (3.4) as a reference for fine bubble section (3.6)
Note 1 to entry: For the purpose of this document, plants grown are lettuces.
3.7
liquid fertilizer
liquid used as nutrient to grow plants
3.8
culture solution
solution containing liquid fertilizer (3.7) supplied to hydroponic cultivation system
3.9
photosynthetic photon flux density
PPFD
number of photons per unit of time and area, which are contained in 400 nm – 700 nm of wave length
needed for photosynthesis
4 Test method for growth promotion performance on lettuce
4.1 Conditions of test environment
4.1.1 Water supply. Sufficient water supply shall be provided to circulate the water in the piping of the
hydroponic bed and the tank.
4.1.2 Air qualit
...
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