ISO 8255-2:2013
(Main)Microscopes — Cover glasses — Part 2: Quality of materials, standards of finish and mode of packaging
Microscopes — Cover glasses — Part 2: Quality of materials, standards of finish and mode of packaging
ISO 8255-2:2013 specifies requirements and methods of test for the quality of material, standards of finish, and mode of packaging for microscope cover glasses. ISO 8255-2:2013 is applicable to microscope cover glasses for use in transmitted light microscopy (400 nm to 760 nm).
Microscopes — Lamelles couvre-objet — Partie 2: Qualité des matériaux, normes de finition et mode d'emballage
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Standards Content (Sample)
INTERNATIONAL ISO
STANDARD 8255-2
Second edition
2013-09-15
Microscopes — Cover glasses —
Part 2:
Quality of materials, standards of
finish and mode of packaging
Microscopes — Lamelles couvre-objet —
Partie 2: Qualité des matériaux, normes de finition et mode d’emballage
Reference number
ISO 8255-2:2013(E)
©
ISO 2013
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ISO 8255-2:2013(E)
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ii © ISO 2013 – All rights reserved
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ISO 8255-2:2013(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 2
4.1 Transparency and colour . 2
4.2 Non-flatness (waviness) and non-parallelism . 2
4.3 Durability . 3
4.4 Surface quality and inclusions . 3
4.5 Edge finish. 3
4.6 Cleanliness and cloudiness . 3
4.7 Adhesion . 3
4.8 Residual stress/birefringence . 3
5 Sampling . 3
5.1 General . 3
5.2 Quality measurement for cover glasses for conformance with this part of ISO 8255 . 3
5.3 Drawing of samples and units inspection . 4
6 Test methods . 4
6.1 General . 4
6.2 Package suitability . 4
6.3 Adhesion . 5
6.4 Cleanliness and cloudiness . 5
6.5 Surface quality and inclusions . 5
6.6 Transparency and colour . 6
6.7 Edge finish. 6
6.8 Non-flatness (waviness) and non-parallelism . 6
6.9 Durability — Chemical durability of surface and resistance to atmospheric
attack (Solubility) . 6
6.10 Residual stress/birefringence . 7
7 Marking/labelling . 7
8 Packaging . 7
Annex A (normative) Sample size code letters and single sampling plans for normal inspection .8
Bibliography .10
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ISO 8255-2:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directives.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received. www.iso.org/patents.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
The committee responsible for this document is ISO/TC 172, Optics and Photonics, Subcommittee SC 5,
Microscopes and endoscopes.
This second edition cancels and replaces the first edition (ISO 8255-2:1997), of which it constitutes a
minor revision.
ISO 8255 consists of the following parts, under the general title Microscopes — Cover glasses:
— Part 1: Dimensional tolerances, thickness and optical properties
— Part 2: Quality of materials, standards of finish and mode of packaging
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ISO 8255-2:2013(E)
Introduction
The data given in this part of ISO 8255 are intended to provide for adequate performance of the product
for the end user. They are applicable to most products in use and have been adapted to take into account
the relevant national standards in force. Dimensions and optical qualities are specified in ISO 8255-1.
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INTERNATIONAL STANDARD ISO 8255-2:2013(E)
Microscopes — Cover glasses —
Part 2:
Quality of materials, standards of finish and mode of
packaging
1 Scope
This part of ISO 8255 specifies requirements and methods of test for the quality of material, standards
of finish, and mode of packaging for microscope cover glasses.
This part of ISO 8255 is applicable to microscope cover glasses for use in transmitted light microscopy
(400 nm to 760 nm).
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 2859-1:1999, Sampling procedures for inspection by attributes — Part 1: Sampling schemes indexed by
acceptance quality limit (AQL) for lot-by-lot inspection
ISO 8255-1:2011, Dimensional tolerances, thickness, and optical properties
ISO 11455:1995, Raw optical glass — Determination of birefringence
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
seed
small bubbles in glass, sometimes elongated
3.2
cord
vitreous compositional inhomogeneities in glass (also known as striae, ream, or glassy knots)
3.3
line
fine parallel line on glass surface in direction of draw
3.4
nick
place where minute piece(s) of glass have been removed from edges of glass, giving rise to poor edge finish
3.5
cleanliness
freedom from visible contamination such as fingerprints, particulate matter, or residue left from
cleaning process
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ISO 8255-2:2013(E)
3.6
cloudiness
haze
light scattering or reduced transparency due to surface deterioration typically as a result of atmospheric
attack in the presence of humidity and CO
2
3.7
abrasion
surface damage and pitting typically caused by vibration of one slide surface on another during packaging
or during shipment and handling
3.8
acceptance quality limit
AQL
quality level that is the worst tolerable process average when a continuing series of lots is submitted for
acceptance sampling
Note 1 to entry: This concept only applies when a sampling scheme with rules for switching and discontinuation,
such as in ISO 2859-1 and in ISO 3951, is used.
Note 2 to entry: Although individual lots with quality as bad as the acceptance quality limit may be accepted
with fairly high probability, the designation of an acceptance quality limit does not suggest that this is a desirable
quality level. Sampling schemes found in International Standards such as this part of ISO 2859, with their rules
for switching and for discontinuation of sampling inspection, are designed to encourage suppliers to have process
averages consistently better than the AQL. Otherwise, there is a high risk that the inspection severity will be
switched to tightened inspection under which the criteria for lot acceptance become more demanding. Once
on tightened inspection, unless action is taken to improve the process, it is very likely that the rule requiring
discontinuation of sampling inspection pending such improvement will be invoked.
[SOURCE: ISO 2859-1:1999, definition 3.1.26]
3.9
thickness variation
difference between the largest and smallest of thickness measurements, within a cover glass
3.10
vision 1,0
standard visual acuity
ability to see an object so small that the angle subtended at the eye is only one minute of arc (1/60 of a degree)
Note 1 to entry: At 0,6 m the size of a test object is about 1,75 mm.
Note 2 to entry: Since slight colour variation is permitted, definition of colour vision quality of the observer is
not critical.
4 Requirements
4.1 Transparency and colour
The cover glass shall be transparent and colourless when observed as specified in 6.6.
4.2 Non-flatness (waviness) and non-parallelism
4.2.1 Non-flatness (waviness)
The cover glass shall be sufficiently free of waviness to pass the test as specified in 6.8.1.
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ISO 8255-2:2013(E)
4.2.2 Non-
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