ISO 7581:2023

Evaluation of bactericidal activity of a non-porous antimicrobial surface used in a dry environment

This document specifies the test conditions and the levels of activity to determine the bactericidal activity of non-porous surfaces used in a dry environment. It defines a protocol to validate the bactericidal character of a surface and to measure its performance. It is not intended to be used to substantiate cleaning or disinfecting properties. This document is applicable to surfaces claiming to have an activity against vegetative bacteria. The obligatory test conditions are defined in this document. It does not apply to porous surfaces. It does not refer to methods for testing the toxicological and ecotoxicological properties of the surfaces. This document is used to measure bactericidal action, not bacteriostatic activity of a surface.

Évaluation de l’activité bactéricide d’une surface antimicrobienne non poreuse utilisée dans un environnement sec

Le présent document spécifie les conditions d’essai et les niveaux d’activité pour déterminer l’activité bactéricide des surfaces non poreuses utilisées dans un environnement sec. Il définit un protocole de validation du caractère bactéricide d’une surface et de mesure de sa performance. Il n’est pas destiné à être utilisé pour démontrer des propriétés nettoyantes ou désinfectantes. Le présent document est applicable aux surfaces revendiquant une activité contre les bactéries végétatives. Les conditions d’essai obligatoires sont définies dans le présent document. Il ne s’applique pas aux surfaces poreuses. Il ne fait pas référence à des méthodes d’essai des propriétés toxicologiques et écotoxicologiques des surfaces. Le présent document permet de mesurer l'action bactéricide et non l'activité bactériostatique d’une surface.

General Information

• Status: Published
• Publication Date: 05-Dec-2023
• ICS: 07.100.99 - Other standards related to microbiology
• Technical Committee: ISO/TC 330 - Surfaces with biocidal and antimicrobial properties
• Drafting Committee: ISO/TC 330 - Surfaces with biocidal and antimicrobial properties
• Current Stage: 6060 - International Standard published
• Start Date: 06-Dec-2023
• Due Date: 04-Oct-2023
• Completion Date: 06-Dec-2023

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 7581
First edition
2023-12
Evaluation of bactericidal activity of
a non-porous antimicrobial surface
used in a dry environment
Évaluation de l’activité bactéricide d’une surface antimicrobienne non
poreuse utilisée dans un environnement sec
Reference number
ISO 7581:2023(E)
© ISO 2023

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ISO 7581:2023(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
  © ISO 2023 – All rights reserved

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ISO 7581:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative reference .1
3 Terms and definitions . 1
4 Apparatus, reagents and materials . 3
4.1 Test organisms . 3
4.2 Culture media and reagents. 3
4.3 Reference and test surfaces . 4
4.3.1 General . 4
4.3.2 Reference surfaces . 4
4.3.3 Test surfaces . . 5
4.4 Apparatus and materials . 6
5 Preparation of the test organism suspensions . 7
5.1 Stock cultures of the test bacteria . 7
5.2 Working culture of the test bacteria . 7
5.3 Test suspensions . 8
5.4 Quantifying of bacterial test suspensions . 8
5.5 Calculation of the weighted mean bacteria concentration in the test suspensions. 8
6 Evaluation method of the bactericidal activity of a non-porous surface and its
efficacy .
...

NORME ISO
INTERNATIONALE 7581
Première édition
2023-12
Évaluation de l’activité bactéricide
d’une surface antimicrobienne
non poreuse utilisée dans un
environnement sec
Evaluation of bactericidal activity of a non-porous antimicrobial
surface used in a dry environment
Numéro de référence
ISO 7581:2023(F)
© ISO 2023

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ISO 7581:2023(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2023
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse
ii
  © ISO 2023 – Tous droits réservés

---------------------- Page: 2 ----------------------
ISO 7581:2023(F)
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d’application . 1
2 Références normatives .1
3 Termes et définitions . 1
4 Appareillage, réactifs et matériels .3
4.1 Organismes d’essai. 3
4.2 Milieux de culture et réactifs . 3
4.3 Surfaces de référence et surfaces d’essai . 4
4.3.1 Généralités . 4
4.3.2 Surfaces de référence . 5
4.3.3 Surfaces d'essai . 5
4.4 Appareillage et matériels . 6
5 Préparation des suspensions de microorganismes d’essai. 7
5.1 Cultures mères des bactéries d’essai . 7
5.2 Culture de travail des bactéries d’essai . 8
5.3 Suspensions d’essai . 8
5.4 Quantification des suspensions bactériennes d’essai . 8
5.5 Calcul de la concentration bactérienne moyenne pondérée dans les suspensions
d’essai . 9
6 Méthode d’évaluation de l’activité bac
...

FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 7581
ISO/TC 330
Evaluation of bactericidal activity of
Secretariat: AFNOR
a non-porous antimicrobial surface
Voting begins on:
2023-09-04 used in a dry environment
Voting terminates on:
2023-10-30
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 7581:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 1 ----------------------
ISO/FDIS 7581:2023(E)
FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 7581
ISO/TC 330
Evaluation of bactericidal activity of
Secretariat: AFNOR
a non-porous antimicrobial surface
Voting begins on:
used in a dry environment
Voting terminates on:
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/FDIS 7581:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 2 ----------------------
ISO/FDIS 7581:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative reference .1
3 Terms and definitions . 1
4 Apparatus, reagents and materials . 3
4.1 Test organisms . 3
4.2 Culture media and reagents. 3
4.3 Reference and test surfaces . 4
4.3.1 General . 4
4.3.2 Reference surfaces . 4
4.3.3 Test surfaces . . 5
4.4 Apparatus and materials .
...

Pr ISO /FDIS 7581:2022(E)
ISO /TC 330
Secretariat: AFNOR
Date: 2022-06-232023-08-18
Evaluation of bactericidal activity of a non-porous antimicrobial
surface used in a dry environment
FDIS stage

---------------------- Page: 1 ----------------------
ISO/DIS 7581:2022(E)
© ISO 20222023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
EmailE-mail: [e-mail]
Website: www.iso.orgwww.iso.org
Published in Switzerland
ii © ISO 2022 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/DIS 7581:2022(E)
Contents
Foreword . 5
Introduction . 6
1 Scope . 1
2 Normative reference . 1
3 Terms and definitions . 1
4 Apparatus, reagents and materials . 3
4.1 Test organisms . 3
4.2 Culture media and reagents . 3
4.3 Reference and test surfaces . 5
4.3.1 General . 5
4.3.2 Reference surfaces . 5
4.3.3 Test surfaces . 5
4.4 Apparatus and materials . 6
5 Preparation of the test organism suspensions . 8
5.1 Stock cultures of the test bacteria . 8
5.2 Working culture of the test bacteria . 8
5.3 Test suspensions . 8
5.4 Quantifying of bacterial test suspensions . 8
5.5 Calculation of the weighted mean bacteria concentration in the test suspensions . 9
6 Evaluation method of the bactericidal activity of a non-porous surface and its efficacy . 10
6.1 Obligatory experimental conditions . 10
6.2 Test procedure . 10
6.2.1 Sampling . 10
6.2.2 Test (T0 and Txh) . 11
6.2.3 Reference surfaces (C and C ) . 12
0 xh
6.2.4 Validation (
...