ISO/TS 7833:2024

Nanotechnologies — Extraction method of nanomaterials from lung tissue by proteinase K digestion

This document provides an extraction method using the proteinase K (PK) for nanomaterials deposited in the lung. This document specifies the advantages of the PK digestion method and examples of nanomaterials to which it can be applied. This document focuses on extracting nanomaterials from lung tissue and separating nanoparticles from their ionic counterparts. This method is potentially (or theoretically) applicable to any particles that are insoluble during the PK digestion process.

Nanotechnologies — Méthode d'extraction de nanomatériaux d'organes par digestion par protéinase K

General Information

• Status: Published
• Publication Date: 30-Jan-2024
• ICS: 07.120 - Nanotechnologies
• Technical Committee: ISO/TC 229 - Nanotechnologies
• Drafting Committee: ISO/TC 229 - Nanotechnologies
• Current Stage: 6060 - International Standard published
• Start Date: 31-Jan-2024
• Due Date: 09-Aug-2024
• Completion Date: 31-Jan-2024

Standards Content (Sample)

FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 7833
ISO/TC 229
Nanotechnologies — Extraction
Secretariat: BSI
method of nanomaterials from lung
Voting begins on:
2023-11-07 tissue by proteinase K digestion
Voting terminates on:
Nanotechnologies — Méthode d'extraction de nanomatériaux
2024-01-02
d'organes par digestion par protéinase K
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 7833:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

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ISO/DTS 7833:2023(E)
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 7833
ISO/TC 229
Nanotechnologies — Extraction
Secretariat: BSI
method of nanomaterials from lung
Voting begins on:
tissue by proteinase K digestion
Voting terminates on:
Nanotechnologies — Méthode d'extraction de nanomatériaux
d'organes par digestion par protéinase K
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/DTS 7833:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

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ISO/DTS 7833:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms.2
5 Materials - PK digestion buffer and optimal concentration for lung tissue digestion .2
6 Technical equipment . 3
6.1 Vessels . 3
6.2 Heat block or water bath . 3
6.3 Drying oven . 3
6.4 Micro ball mill .
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ISO/DTS 7833:2023(E) .
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ISO/TC 229 /WG 3
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Secretariat: BSI
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Date: 2023-09-07xx
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Nanotechnologies — Extraction method of nanomaterials from
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lung tissue by proteinase K digestion .
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Nanotechnologies — Méthode d'extraction de nanomatériaux d'organes par digestion par protéinase K
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ISO/DTS 7833:2023(E)
Formatted: Font: 11 pt, Bold
Formatted: Font: Bold
Formatted: Left: 1.9 cm, Right: 1.9 cm, Bottom: 1 cm,
© ISO 2023
Gutter: 0 cm, Header distance from edge: 1.27 cm,
Footer distance from edge: 0.5 cm
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this
publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical,
Formatted: Default Paragraph Font
including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can
Formatted: Default Paragraph Font
be requested from either ISO at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
Formatted: French (Switzerland)
EmailE-mail: [e-mail]
Formatted: French (Switzerland)
Website: www.iso.orgwww.iso.org
Formatted: French (Switzerland)
Published in Switzerland
ii © ISO 2023 – All rights reserved
ii © ISO 2023 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/DTS 7833:2023(E)
Formatted: Font: 11 pt, Bold
Formatted: Font: Bold
Formatted: Left
Contents
Foreword . v
Introduction.vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms. 2
5 Materials - PK digestion buffer and optimal concentration for lung tissue digestion . 2
6 Technical equipment . 3
6.1 Vessels . 3
6.2 Heat block or water bath . 3
6.3 Drying oven . 3
6.4 Micro ball mill . 3
6.5 Microcentrifuge or ultracentrifuge . 3
6.6 Bath sonicator .
...