ISO 18187:2024

Soil quality — Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis

This document specifies a rapid method for assessing solid samples in an aerobic suspension, by determining the inhibition of dehydrogenase activity of Arthrobacter globiformis using the redox dye resazurin. It is applicable for assessing the effect of water-soluble and solid matter bounded non-volatile contaminants in natural samples, such as soils and waste materials. Although not the main purpose, the contact test can additionally be used for testing the effect of chemicals, as described in the Annex C. The test yields a result within 6 h and can therefore be used for screening potentially contaminated test material.

Qualité du sol — Essai contact pour échantillons solides utilisant l'activité déshydrogénase de Arthrobacter globiformis

Le présent document spécifie une méthode rapide d’évaluation d’échantillons solides en suspension aérobie, en déterminant l’inhibition de l’activité déshydrogénase de Arthrobacter globiformis à l’aide d’un indicateur redox coloré, la résazurine. Cette méthode est applicable à l’évaluation de l’effet de contaminants non volatils, liés aux matières solides ou solubles dans l’eau, présents dans des échantillons naturels tels que des sols et des déchets. Bien qu’il ne s’agisse pas de sa principale finalité, l’essai contact peut également être utilisé pour l’évaluation des effets des produits chimiques, comme décrit dans l’Annexe C. L’essai permet d’obtenir un résultat en 6 h et peut donc être utilisé pour le criblage de matériaux d’essai potentiellement contaminés.

General Information

• Status: Published
• Publication Date: 13-May-2024
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: ISO/TC 190/SC 4 - Biological characterization
• Drafting Committee: ISO/TC 190/SC 4 - Biological characterization
• Current Stage: 6060 - International Standard published
• Start Date: 14-May-2024
• Due Date: 20-Oct-2024
• Completion Date: 14-May-2024

Relations

• Consolidates: ISO 23234:2021 - Buildings and civil engineering works — Security — Planning of security measures in the built environment
• Effective Date: 06-Jun-2022
• Revises: ISO 18187:2016 - Soil quality — Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis
• Effective Date: 06-Jun-2022

Standards Content (Sample)

International
Standard
ISO 18187
Second edition
Soil quality — Contact test for solid
2024-05
samples using the dehydrogenase
activity of Arthrobacter globiformis
Qualité du sol — Essai contact pour échantillons solides utilisant
l'activité déshydrogénase de Arthrobacter globiformis
Reference number
ISO 18187:2024(en) © ISO 2024

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ISO 18187:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland

© ISO 2024 – All rights reserved
ii

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ISO 18187:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 Reagents and material . 4
5.1 Test organisms .4
5.2 Control substrates .4
5.2.1 General .4
5.2.2 Control for soils .4
5.2.3 Control for waste material .5
5.3 Test substrates . . .5
5.4 Reagents .6
6 Apparatus . 8
7 Procedure . 9
7.1 Preparation of dilutions .
...

Norme
internationale
ISO 18187
Deuxième édition
Qualité du sol — Essai contact
2024-05
pour échantillons solides utilisant
l'activité déshydrogénase de
Arthrobacter globiformis
Soil quality — Contact test for solid samples using the
dehydrogenase activity of Arthrobacter globiformis
Numéro de référence
ISO 18187:2024(fr) © ISO 2024

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ISO 18187:2024(fr)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2024
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse

© ISO 2024 – Tous droits réservés
ii

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ISO 18187:2024(fr)
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe. 4
5 Réactifs et matériaux . 4
5.1 Micro-organismes d’essai .4
5.2 Substrats témoins .5
5.2.1 Généralités .5
5.2.2 Témoin pour les sols .5
5.2.3 Témoin pour les déchets .6
5.3 Substrats d’essai .6
5.4 Réactifs .7
6 Appareillage . 9
7 Mode opératoire . 10
7.1 Préparation des dilutions .10
7.2 Préparation de
...

FINAL DRAFT
International
Standard
ISO/FDIS 18187
ISO/TC 190/SC 4
Soil quality — Contact test for solid
Secretariat: AFNOR
samples using the dehydrogenase
Voting begins on:
activity of Arthrobacter globiformis
2024-02-16
Qualité du sol — Essai contact pour échantillons solides utilisant
Voting terminates on:
l'activité déshydrogénase de Arthrobacter globiformis
2024-04-12
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 18187:2024(en) © ISO 2024

---------------------- Page: 1 ----------------------
FINAL DRAFT
ISO/FDIS 18187:2024(en)
International
Standard
ISO/FDIS 18187
ISO/TC 190/SC 4
Soil quality — Contact test for solid
Secretariat: AFNOR
samples using the dehydrogenase
Voting begins on:
activity of Arthrobacter globiformis
Qualité du sol — Essai contact pour échantillons solides utilisant
Voting terminates on:
l'activité déshydrogénase de Arthrobacter globiformis
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
COPYRIGHT PROTECTED DOCUMENT
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2024
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 18187:2024(en) © ISO 2024

© ISO 2024 – All rights reserved
ii

---------------------- Page: 2 ----------------------
ISO/FDIS 18187:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 Reagents and material . 4
5.1 Test organisms .4
5.2 Control substrates .4
5.2.1 General .
...

ISO/DIS FDIS 18187:2023(E)
ISO/TC 190/SC 4/WG 4
Secretariat: AFNOR
Date: 2023-09-182024-02-02
Soil quality — Contact test for solid samples using the dehydrogenase
activity of Arthrobacter globiformis
Qualité du sol — Essai de contact pour des échantillons solides en utilisant l’activitél'activité déshydrogénase de
Arthrobacter globiformis
FDIS stage

---------------------- Page: 1 ----------------------
ISO/DISFDIS 18187:2023(E2024(en)
© ISO 20232024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
EmailE-mail: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2023 – All rights reserved
© ISO 2024 – All rights reserved

ii

---------------------- Page: 2 ----------------------
ISO/DISFDIS 18187:2023(E2024(en)
Contents
Foreword . v
Introduction .vi i
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 Reagents and material . 4
5.1 Test organisms . 4
5.2 Control substrates . 5
5.2.1 General . 5
5.2.2 Control for soils . 5
5.2.3 Control for waste material . 6
5.3 Test substrates . 6
5.4 Reagents . 7
6 Apparatus . 9
7 Procedure . 10
7.1 Preparation of dilutions . 10
7.2 Reference substances and positive control preparation . 10
7.3 Contact test procedure . 11
7.3.1 General .
...