ISO/TS 20224-11:2024
(Main)Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 11: Pigeon DNA detection method
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 11: Pigeon DNA detection method
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of pigeon-specific DNA derived from food and feed. The method can be applied to distinguish rock pigeon (Columba livia) from other domestic poultry meats (e. g. goose, duck, quail, pheasant). It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix. The target sequence is a partial fragment of Columba livia breed Danish Tumbler unplaced genomic scaffold, Cliv_1.0 scaffold114 (i.e. GenBank accession number NW_004973337.1),[1] which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).
Analyse de biomarqueurs moléculaires — Détection de matériaux d'origine animale dans les denrées alimentaires et les aliments pour animaux par PCR en temps réel — Partie 11: Méthode de détection de l'ADN de pigeon
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ISO/TS 20224-11
First edition
Molecular biomarker analysis —
2024-02
Detection of animal-derived
materials in foodstuffs and
feedstuffs by real-time PCR —
Part 11:
Pigeon DNA detection method
Analyse de biomarqueurs moléculaires — Détection de matériaux
d'origine animale dans les denrées alimentaires et les aliments
pour animaux par PCR en temps réel —
Partie 11: Méthode de détection de l'ADN de pigeon
Reference number
ISO/TS 20224-11:2024(en) © ISO 2024
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ISO/TS 20224-11:2024(en)
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Published in Switzerland
© ISO 2024 – All rights reserved
ii
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ISO/TS 20224-11:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Scientific basis . 2
5 Reagents and materials . 2
5.1 General .2
5.2 PCR reagents .2
5.2.1 PCR master mix. .2
5.2.2 Oligonucleotides. .2
6 Apparatus . 3
6.1 Real-time thermocycler instrument. .3
7 Procedure . 3
7.1 Preparation of the test portion/sample .3
7.2 Preparation of DNA extracts .3
7.3 PCR setup.
...
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 20224-11
ISO/TC 34/SC 16
Molecular biomarker analysis —
Secretariat: ANSI
Detection of animal-derived materials
Voting begins on:
2023-10-19 in foodstuffs and feedstuffs by real-
time PCR —
Voting terminates on:
2023-12-14
Part 11:
Pigeon DNA detection method
Analyse de biomarqueurs moléculaires — Détection de matériaux
d'origine animale dans les denrées alimentaires et les aliments pour
animaux par PCR en temps réel —
Partie 11: Méthode de détection de l'ADN de pigeon
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 20224-11:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023
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ISO/DTS 20224-11:2023(E)
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 20224-11
ISO/TC 34/SC 16
Molecular biomarker analysis —
Secretariat: ANSI
Detection of animal-derived materials
Voting begins on:
in foodstuffs and feedstuffs by real-
time PCR —
Voting terminates on:
Part 11:
Pigeon DNA detection method
Analyse de biomarqueurs moléculaires — Détection de matériaux
d'origine animale dans les denrées alimentaires et les aliments pour
animaux par PCR en temps réel —
Partie 11: Méthode de détection de l'ADN de pigeon
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: +41 22 749 01 11
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: copyright@iso.org
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 20224-11:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
© ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 2 ----------------------
ISO/DTS 20224-11:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Scientific basis . 2
5 Reagents and materials . 2
5.1 General . 2
5.2 PCR reagents . 2
5.2.1 PCR master mix. .
...
ISO/CD DTS 20224-11:2023(E)
ISO /TC 34/SC 16/WG 8
Secretariat: ANSI
Date: 2023-10-05
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and
feedstuffs by real-time PCR — Part 11: Pigeon DNA detection method
Analyse de biomarqueurs moléculaires — Détection de matériaux d'origine animale dans les denrées
alimentaires et les aliments pour animaux par PCR en temps réel — Partie 11: Méthode de détection de
l'ADN de pigeon
CD stage
Warning for WDs and CDs
This document is not an ISO International Standard. It is distributed for review and comment. It is subject to
change without notice and may not be referred to as an International Standard.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of
which they are aware and to provide supporting documentation.
ii
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© ISO 2020
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ISO/TS DTS 20224-11:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no
part of this publication may be reproduced or utilized otherwise in any form or by any means,
electronic or mechanical, including photocopying, or posting on the internet or an intranet, without
prior written permission. Permission can be requested from either ISO at the address below or
ISO’s member body in the country of the requester.
ISO Copyright Office
CP 401 • CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
Email: copyright@iso.org
Email: copyright@iso.org
Website: www.iso.org www.iso.org
Published in Switzerland.
ii © ISO 2022 – All rights reserved
ii © ISO 2023 – All rights reserved
---------------------- Page: 3 ----------------------
ISO/TS DTS 20224-11:2023(E)
Contents
Foreword . vi
Introduction . vii
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Scientific basis . 1
5 Reagents and materials . 2
5.1 General . 2
5.2 PCR reagents . 2
6 Apparatus . 3
6.1 Real-time thermocycler instrument. . 3
7 Procedure . 3
7.1 Preparation of the test portion/sample . 3
7.2 Preparation of DNA extracts . 3
7.3 PCR setup . 3
7.4 Temperature-time programme. 4
8 Accept/reject criteria . 5
8.1 General . 5
8.2 Identification . 5
9 Validation status and performance criteria. 5
9.1 General .
...
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