ISO 11816-2:2024

Milk and milk products — Determination of alkaline phosphatase activity — Part 2: Fluorimetric method for cheese

This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in cheese. This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7). The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits per litre (mU/kg).

Lait et produits laitiers — Détermination de l'activité de la phosphatase alcaline — Partie 2: Méthode fluorimétrique pour le fromage

Le présent document spécifie une méthode fluorimétrique de détermination de l’activité de la phosphatase alcaline (ALP) (EC 3.1.3.1) dans le fromage. La présente méthode est applicable aux fromages à pâte molle, pâte pressée non cuite et pâte pressée cuite, à condition que la moisissure ne soit présente que sur la surface du fromage et ne se soit pas propagée à l’intérieur, comme dans le cas des fromages veinés de bleu, par exemple. Pour les gros fromages à pâte pressée cuite, des conditions d’échantillonnage spécifiques s’appliquent (voir l’Article 7). L’instrument utilisé pour la détermination de l’ALP peut lire des activités jusqu’à 7 000 mU/kg (milliunités par kilogramme).

General Information

• Status: Published
• Publication Date: 11-Jan-2024
• ICS: 67.100.30 - Cheese
• Technical Committee: ISO/TC 34/SC 5 - Milk and milk products
• Drafting Committee: ISO/TC 34/SC 5 - Milk and milk products
• Current Stage: 6060 - International Standard published
• Start Date: 12-Jan-2024
• Due Date: 27-Oct-2023
• Completion Date: 12-Jan-2024

Relations

• Consolidates: ISO 28596:2022 - Sampling procedures for inspection by attributes — Two-stage sampling plans for auditing and for inspection under prior information
• Effective Date: 08-Oct-2022
• Revises: ISO 11816-2:2016 - Milk and milk products — Determination of alkaline phosphatase activity — Part 2: Fluorimetric method for cheese
• Effective Date: 01-Oct-2022

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 11816-2
IDF 155-2
Third edition
Milk and milk products —
Determination of alkaline
phosphatase activity —
Part 2:
Fluorimetric method for cheese
Lait et produits laitiers — Détermination de l'activité de la
phosphatase alcaline —
Partie 2: Méthode fluorimétrique pour le fromage
PROOF/ÉPREUVE
Reference numbers
ISO 11816-2:2023(E)
IDF 155-2:2023(E)
© ISO and IDF 2023

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ISO 11816-2:2023(E)
IDF 155-2:2023(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: [phone] Phone: [phone]
Fax: [phone]
Email: [e-mail] Email: [e-mail]
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
PROOF/ÉPREUVE © ISO and IDF 2023 – All rights reserved

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ISO 11816-2:2023(E)
IDF 155-2:2023(E)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure .5
9.1 Verification of instrument performance . 5
9.1.1 General . 5
9.1.2 Daily instrument tests . 5
9.1.3 Using FLM200 . 5
9.1.4 Using FLM300 . 5
9.1.5 Controls . 6
9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3) . 6
9.3 Calibrati
...

© ISO and IDF 2023 – All rights reserved
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Date: 2023-08-0110-10
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Secretariat: NEN
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Milk and milk products — Determination of alkaline phosphatase activity — Part 2: Fluorimetric
method for cheese
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Lait et produits laitiers — Détermination de l’activité de la phosphatase alcaline — Partie 2: Style Definition: Heading 6: Font: Bold
Méthode fluorimétrique pour le fromage
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© ISO and IDF 2023 – All rights reserved
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this
publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical,
including photocopying, or posting on the internet or an intranet, without prior written permission. Permission
can be requested from either ISO at the address below or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: [phone] Phone: + 32 2 325 67 40
Email: [e-mail] Fax: + 32 2 325 67 41
Website: www.iso.org Email: [e-mail]
Website: www.fil-idf.org
Published in Switzerland

---------------------- Page: 2 ----------------------
ISO #####-#:####(X)
Contents
Forewords . iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure . 5
10 Calculation and expression of results . 10
11 Precision . 11
12 Test report . 11
Annex A (informative) Interlaboratory trial . 13
Annex B (informative) Examples of preparation of a test s
...