ISO/TS 4985:2023

Milk and milk products — Determination of alkaline phosphatase activity — Fluorimetric microplate method

This document specifies a fluorimetric microplate method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1)[5] activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk, cream, flavoured milks and cheeses. This method is applicable to milk and milk-based drinks from cows, sheep and goats. Although the method was not tested in milk from other species, it can also be applicable to milk from other species with a similar composition to cow, sheep or goat milk, such as milk from buffalo and camelids. It is also applicable to milk powder after reconstitution and soft, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7). NOTE This method was adapted from Reference [6].

Lait et produits laitiers — Détermination de l’activité de la phosphatase alcaline — Méthode fluorimétrique sur microplaque

Le présent document spécifie une méthode fluorimétrique sur microplaque pour la détermination de l’activité de la phosphatase alcaline (ALP, EC 3.1.3.1)[5] dans les laits crus et traités thermiquement entiers, demi-écrémés, écrémés, dans la crème, les laits aromatisés et les fromages. La méthode est applicable au lait et aux boissons à base de lait de vache, de brebis et de chèvre. Bien que la méthode n’ait pas été testée sur le lait d’autres espèces, elle peut également s’appliquer au lait d’espèces dont la composition est semblable à celle du lait de vache, de brebis ou de chèvre, tel que le lait de bufflonne ou de camélidés. Elle est également applicable au lait en poudre après reconstitution et aux fromages à pâte molle, à pâte pressée non cuite et à pâte pressée cuite, à condition que la moisissure ne soit présente que sur la surface du fromage et ne se soit pas propagée à l’intérieur, comme dans le cas des fromages veinés de bleu, par exemple. Pour les gros fromages à pâte pressée cuite, des conditions spécifiques d’échantillonnage s’appliquent (voir Article 7). NOTE Cette méthode a été adaptée de la Référence [6].

General Information

• Status: Published
• Publication Date: 30-Jul-2023
• ICS: 67.100.10 - Milk and processed milk products
• Technical Committee: ISO/TC 34/SC 5 - Milk and milk products
• Drafting Committee: ISO/TC 34/SC 5 - Milk and milk products
• Current Stage: 6060 - International Standard published
• Start Date: 31-Jul-2023
• Due Date: 29-Apr-2023
• Completion Date: 31-Jul-2023

Standards Content (Sample)

TECHNICAL ISO/TS
SPECIFICATION 4985
IDF/RM 255
First edition
2023-07
Milk and milk products —
Determination of alkaline
phosphatase activity — Fluorimetric
microplate method
Reference numbers
ISO/TS 4985:2023(E)
IDF/RM 255:2023(E)
© ISO and IDF 2023

---------------------- Page: 1 ----------------------
ISO/TS 4985:2023(E)
IDF/RM 255:2023(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: [phone] Phone: [phone]
Fax: [phone]
Email: [e-mail] Email: [e-mail]
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
  © ISO and IDF 2023 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/TS 4985:2023(E)
IDF/RM 255:2023(E)
Contents Page
Forewords .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus and materials .3
7 Sampling . 4
8 Preparation . 4
8.1 Preparation of alkaline phosphatase-free sample . 4
8.1.1 General . 4
8.1.2 Alkaline phosphatase-free milk . 4
8.1.3 Alkaline phosphatase-free cheese . 4
8.2 Preparation of test sample . 5
8.2.1 Milk samples. 5
8.2.2 Cheese samples . 5
9 Procedure .5
9.1 Instrument .
...

FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 4985
IDF/RM 255
ISO/TC 34/SC 5
Milk and milk products —
Secretariat: NEN
Determination of alkaline
Voting begins on:
2023-04-19 phosphatase activity — Fluorimetric
microplate method
Voting terminates on:
2023-06-14
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference numbers
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 4985:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
IDF/RM 255:2023(E)
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO and IDF 2023

---------------------- Page: 1 ----------------------
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 4985
IDF/RM 255
ISO/TC 34/SC 5
Milk and milk products —
Secretariat: NEN
Determination of alkaline
Voting begins on:
phosphatase activity —
Fluorimetric microplate method
Voting terminates on:
COPYRIGHT PROTECTED DOCUMENT
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
RECIPIENTS OF THIS DRAFT ARE INVITED TO
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CH-1214 Vernier, Geneva B-1030 Brussels
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
Phone: [phone] Phone: [phone]
DOCUMENTATION.
Fax: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference numbers
Email: [e-mail] Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/DTS 4985:2023(E)
Website: www.iso.org Website: www.fil-idf.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
IDF/RM 255:2023(E)
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO and IDF 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO and IDF 2023

---------------------- Page: 2 ----------------------
ISO/DTS 4985:2023(E)
IDF/RM 255:2023(E)
Contents Page
Forewords .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus and materials .3
7 Sampling . 4
8 Preparation . 4
8.1 Preparation of alkaline phosphatase­free sample . 4
8.1.1 General .
...

© ISO and IDF 2023 – All rights reserved
ISO/DTS 4985 | IDF/RM 255:2023(E)
Date: 2023-01-2704-04
ISO/TC 34/SC 5
Secretariat: NEN
Milk and milk products — Determination of alkaline phosphatase
activity — Fluorimetric microplate method

---------------------- Page: 1 ----------------------
ISO/DTS 4985 | IDF/RM 255:2023(E)
© ISO and IDF 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no
part of this publication may be reproduced or utilized otherwise in any form or by any means,
electronic or mechanical, including photocopying, or posting on the internet or an intranet, without
prior written permission. Permission can be requested from either ISO at the address below or
ISO’s member body in the country of the requester.
ISO copyright office                        International Dairy Federation
CP 401 • Ch. de Blandonnet                 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva                   B-1030 Brussels
Phone: [phone]                   Phone: + 32 2 325 67 40
                                           Fax: + 32 2 325 67 41
Email: [e-mail]                   Email: [e-mail]
Website: www.iso.org                      Website: www.fil-idf.org
Published in Switzerland.
ii © ISO and IDF 2023 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/DTS 4985 | IDF/RM 255:2023(E)
Contents
Forewords . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus and materials . 3
7 Sampling . 4
8 Preparation . 4
8.1 Preparation of alkaline phosphatase-free sample . 4
8.1.1 General . 4
8.1.2 Alkaline phosphatase-free milk . 5
8.1.3 Alkaline phosphatase-free cheese . 5
8.2 Preparation of test sample . 5
8.2.1 Milk samples . 5
8.2.2 Cheese samples . 5
9 Procedure . 6
9.1 Instrument . 6
9.1.1 Instrument settings . 6
9.1.2 Test the linearity range of the instrument . 6
9.1.3 Quality control .
...