ISO/FDIS 25184
(Main)Molecular biomarker analysis - Nucleotide sequencing - Verified next generation sequences (VNGS)
Molecular biomarker analysis - Nucleotide sequencing - Verified next generation sequences (VNGS)
This document provides requirements for reference nucleotide sequences. This document applies to all nucleotide sequences determined by next-generation sequencing (NGS) technology that are accessible on the semantic web and included in a genetic database (public or private).
Titre manque
General Information
Standards Content (Sample)
FINAL DRAFT
International
Standard
ISO/TC 34/SC 16
Molecular biomarker analysis —
Secretariat: ANSI
Nucleotide sequencing — Verified
Voting begins on:
next generation sequences (VNGS)
2026-01-05
Voting terminates on:
2026-03-02
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
FINAL DRAFT
International
Standard
ISO/TC 34/SC 16
Molecular biomarker analysis —
Secretariat: ANSI
Nucleotide sequencing — Verified
Voting begins on:
next generation sequences (VNGS)
2026-01-05
Voting terminates on:
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ii
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 5
5 VNGS requirements . 5
5.1 General .5
5.2 VNGS use case.6
6 VNGS identifier scheme . 6
6.1 VNGS URI .6
6.2 Version control .6
6.3 VNGS format .6
6.4 Semantic interoperability .7
6.5 Knowledge representation .7
7 VNGS technical and organizational requirements . 7
7.1 Establishment, maintenance and changes to the VNGS .7
7.2 Delegation of user requests . . .7
7.3 Updates to VNGS standard requirements .7
7.4 Documentation .7
7.5 Compatibility, extensibility and compression .7
7.6 Data types .7
7.7 Format validation .8
7.8 Data versioning and provenance .8
7.9 Data structure .8
7.10 Ontology requirements .8
7.11 Minimum annotation information .8
7.12 Language .8
7.13 Domain.8
7.14 Stable URIs and versioning .8
7.15 Raw sequence data.8
7.16 Aligned sequence data .8
7.17 Annotation formats .9
7.18 Sequence instrument quality metrics . . .9
7.18.1 Base quality score .9
7.18.2 Artefacts .9
7.18.3 Sequencing platform specific error profiles .9
7.18.4 Variation in quality scores across the sequence read .9
7.18.5 Biases in sequence data driven by base composition .9
7.18.6 Departure from suboptimal library fragment sizes .9
7.18.7 Contamination from known and unknown species other than the sequencing
target.9
7.18.8 Insert size .9
7.18.9 Number of reads .9
7.18.10 Base calling.9
7.18.11 Sequence length distribution .9
7.18.12 Length of longest contig .9
7.18.13 N50 .10
7.18.14 NG50 .10
7.18.15 Number of contigs .10
7.18.16 Base composition .10
7.18.17 Coverage . .10
iii
7.18.18 Breadth of coverage .10
7.18.19 Cluster density .10
Annex A (informative) Implementation of a VNGS approach .11
Bibliography .13
iv
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent rights
in respect thereof. As of the date of publication of this document, ISO had not received notice of (a) patent(s)
which may be required to implement this document. However, implementers are cautioned that this may not
represent the latest information, which may be obtained from the patent database available at www.iso.org/
patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 16,
Horizontal methods for molecular biomarker analysis, in collaboration with AOAC INTERNATIONAL. It
is being published by ISO and separately by AOAC INTERNATIONAL. The requirements described in this
document are equivalent to the Official Methods of Analysis of AOAC INTERNATIONAL, Appendix T, Standard
Requirements for Nucleotide Sequences used in Biothreat Agent Detection, Identification, and Quantification:
Verified New Generation Sequences (VNGS).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
v
Introduction
Nucleotide sequencing is cross-cutting across biomolecular methods in agriculture, food production and
the life sciences. Bioinformatic analytical methods and pipelines require reliable reference sequences for
comparison with unknown sample sequences. The verified next generation sequence (VNGS) concept was
developed by the AOAC INTERNATIONAL. It is designed to provide the requirements necessary for a reliable
and accurately determined new generation reference sequence. This document provides the requirements
for a VNGS.
vi
FINAL DRAFT International Standard ISO/FDIS 25184:2026(en)
Molecular biomarker analysis — Nucleotide sequencing —
Verified next generation sequences (VNGS)
1 Scope
[1][2]
This document specifies requirements for reference next generation nucleotide sequences.
This document is applicable to all verified next generation (VNGS) nucleotide sequences determined by next
generation sequence (NGS) technology that are accessible on the semantic web and included in a database
[3][4][5][6]
(public or private).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 16577, Molecular biomarker analysis — Vocabulary for molecular biomarker analytical methods in
agriculture and food production
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 16577 and the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
alignment
sequence alignment
arrangement of nucleotide sequences (3.27) so that regions of similarity versus dissimilarity, polymorphic
regions and haplotype are shown
3.2
ASCII character set
American Standard Code for Information Interchange character set
character encoding standard for electronic communication
3.3
assemblies
set of DNA segments or sequences that overlap in a way that provides a contiguous representation of a
genomic region
3.4
base calling
computational process in massively parallel sequencing for translating raw optical and electrical signals to
nucleotide sequence (3.27)
3.5
GC composition
GC content
percentage of GC base pairs in the genome
3.6
breadth of coverage
percentage of genome bases that are sequenced a given number of times
EXAMPLE A genome sequenced to 30× average depth, achieving a 95% breadth of coverage of the reference
genome at a minimum depth of 10 reads.
[7]
[SOURCE: Reference ]
3.7
cluster density
number of templates present on a next generation sequencing cell/chip
3.8
context
circumstance, purpose, and perspective under which an object is defined or used
3.9
contig
continuous stretch of DNA sequence that results from the assembly of smaller, overlapping DNA sequence
reads
3.10
coverage
number of times that a given base or k-mer position is read in a sequencing run given base position or across
a sliding window of multiple bases
[8] [9]
Note 1 to entry: See References and .
3.11
detection
recognition of the presence of the target nucleic acid
3.12
extensible markup language
XML
markup language that encodes information in a way that is machine processable as well as human-readable
3.13
FAST5
standard sequencing output for nanopore sequencers
3.14
FASTQ
text-based format for storing both a biological sequence (usually nucleotide sequence (3.27)) and its
corresponding quality scores
Note 1 to entry: Both the sequence letter and quality score are encoded with a single American Standard Code for
Information Interchange (ASCII) character for brevity.
3.15
forward compatibility
design that is compatible with future versions of the article
3.16
backward compatibility
design that is compatible with previous versions of the article
3.17
identification
establishment of the identity of an organism or virus by next generation sequence (NGS) (3.25) analysis
3.18
insert size
length of the sequence between the sequencing adapters
[10]
[SOURCE: Reference ]
3.19
JavaScript object notation
JSON
open and text-based exchange format
3.20
knowledge representation
process or result of encoding and storing information so that it can be interpreted and used by a computer
3.21
length of longest contig
size of the longest consensus region of DNA produced from a set of overlapping DNA segment reads
3.22
machine readable
data in a format that is easily interpretable by a machine while not necessarily recognizable by humans
3.23
metadata
data that describe other data
3.24
N50
fifty per cent of the entire next generation sequence (NGS) (3.25) assembly that is contained in contigs (3.9) or
scaffolds equal to or larger than this value in base length
Note 1 to entry: Individual sequencing reads are processed to remove linkers and barcodes and then assembled into
overlapping contigs. The next step is to assemble the contigs into progressively larger scaffolds by bridging the gaps
between contigs with additional sequence reads until the entire genome is assembled.
3.25
next generation sequence
NGS
nucleotide sequence (3.27) produced using a massively parallel sequencing methodology
3.26
NG50
N50 (3.24) except the metric relates to the genome size rather than the assembly size
[11]
[SOURCE: Reference ]
3.27
nucleotide sequence
order of purine or pyrimidine nucleosides linked by a phosphodiester backbone and hydrogen bonding in a
deoxyribonucleotide or ribonucleotide molecule
3.28
number of contigs
total count of contiguous DNA sequences after assembly of the whole genome sequence
3.29
number of reads
total quantity of sequenced DNA fragments, or reads, generated during a sequencing run
3.30
OBO Foundry
Open Biological and Biomedical Ontologies Foundry
open biological and biomedical ontology
3.31
ontology
logical structure of the terms used to describe a domain of knowledge, including both the definitions of the
applicable terms and their relationships
3.32
Ontology Web Language
OWL
family of knowledge representation (3.20) or ontology (3.31) languages for authoring ontologies or knowledge
bases
3.33
pod5
specialized nucleotide sequence (3.27) format interconvertible with FASTA, consisting of universally unique
identifiers based on a custom container with the extension
Note 1 to entry: pod 5 is usually used for nanopore based sequencing.
3.34
quantification
determination of the amount of biological material in a sample
3.35
raw read
initial, unaligned data generated by a sequencing instrument
3.36
reifiable
capable of being made more concrete or real
3.37
resource description framework
RDF
extensible markup language (XML) syntax for describing metadata (3.23)
3.38
responsible party
perso
...
ISO/TC 34/SC 16
Secretariat: ANSI
Date: 2025-11-12-19
Molecular biomarker analysis — Nucleotide sequencing — Verified
next generation sequences (VNGS)
FDIS stage
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
E-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 5
5 VNGS requirements . 6
5.1 General . 6
5.2 VNGS use case . 6
6 VNGS identifier scheme . 6
6.1 VNGS URI . 6
6.2 Version control . 7
6.3 VNGS format . 7
6.4 Semantic interoperability . 7
6.5 Knowledge representation . 7
7 VNGS technical and organizational requirements . 7
7.1 Establishment, maintenance and changes to the VNGS . 7
7.2 Delegation of user requests . 7
7.3 Updates to VNGS standard requirements . 7
7.4 Documentation . 7
7.5 Compatibility, extensibility and compression . 8
7.6 Data types . 8
7.7 Format validation . 8
7.8 Data versioning and provenance . 8
7.9 Data structure . 8
7.10 Ontology requirements . 8
7.11 Minimum annotation information . 8
7.12 Language . 8
7.13 Domain . 9
7.14 Stable URIs and versioning . 9
7.15 Raw sequence data . 9
7.16 Aligned sequence data . 9
7.17 Annotation formats . 9
7.18 Sequence instrument quality metrics . 9
Annex A (informative) Implementation of a VNGS approach . 11
Bibliography . 13
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types of
ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent rights
in respect thereof. As of the date of publication of this document, ISO had not received notice of (a) patent(s)
which may be required to implement this document. However, implementers are cautioned that this may not
represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 16,
Horizontal methods for molecular biomarker analysis, in collaboration with AOAC INTERNATIONAL. It is being
published by ISO and separately by AOAC INTERNATIONAL. The requirements described in this document are
equivalent to the Official Methods of Analysis of AOAC INTERNATIONAL, Appendix T, Standard Requirements
for Nucleotide Sequences used in Biothreat Agent Detection, Identification, and Quantification: Verified New
Generation Sequences (VNGS).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
Nucleotide sequencing is cross-cutting across biomolecular methods in agriculture, food production and the
life sciences. Bioinformatic analytical methods and pipelines require reliable reference sequences for
comparison with unknown sample sequences. The verified next generation sequence (VNGS) concept was
developed by the AOAC INTERNATIONAL. It is designed to provide the requirements necessary for a reliable
and accurately determined new generation reference sequence. This document provides the requirements for
a VNGS.
v
Molecular biomarker analysis — Nucleotide sequencing — Verified
next generation sequences (VNGS)
1 Scope
[1][2]
This document providesspecifies requirements for reference next generation nucleotide sequences.
This document is applicable to all verified next generation (VNGS) nucleotide sequences determined by next
generation sequence (NGS) technology that are accessible on the semantic web and included in a database
[3][4][5][6]
(public or private).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 16577, Molecular biomarker analysis — Vocabulary for molecular biomarker analytical methods in
agriculture and food production
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 16577 and the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp
— IEC Electropedia: available at https://www.electropedia.org/
3.1
alignment
sequence alignment
arrangement of nucleotide sequences (3.27) so that regions of similarity versus dissimilarity, polymorphic
regions and haplotype are shown
3.2
ASCII character set
American Standard Code for Information Interchange character set
character encoding standard for electronic communication
3.3
assemblies
set of DNA segments or sequences that overlap in a way that provides a contiguous representation of a
genomic region
3.4
base calling
computational process in massively parallel sequencing for translating raw optical and electrical signals to
nucleotide sequence (3.27)
3.5
GC composition
GC content
percentage of GC base pairs in the genome
3.6
breadth of coverage
percentage of genome bases that are sequenced a given number of times
EXAMPLE A genome sequenced to 30× average depth, achieving a 95% breadth of coverage of the reference genome
at a minimum depth of 10 reads.
[7]
[SOURCE: Reference This reference is informative]]
3.7
cluster density
number of templates present on a next generation sequencing cell/chip
3.8
context
circumstance, purpose, and perspective under which an object is defined or used
3.9
contig
continuous stretch of DNA sequence that results from the assembly of smaller, overlapping DNA sequence
reads
3.10
coverage
number of times that a given base or k-mer position is read in a sequencing run given base position or across
a sliding window of multiple bases
[8] [9]
Note 1 to entry: See References and .
3.11
detection
recognition of the presence of the target nucleic acid
3.12
extensible markup language
XML
markup language that encodes information in a way that is machine processable as well as human-readable
3.13
FAST5
standard sequencing output for nanopore sequencers
3.14
FASTQ
text-based format for storing both a biological sequence (usually nucleotide sequence (3.27)) and its
corresponding quality scores
Note 1 to entry: Both the sequence letter and quality score are encoded with a single American Standard Code for
Information Interchange (ASCII) character for brevity.
3.15
forward compatibility
design that is compatible with future versions of the article
3.16
backward compatibility
design that is compatible with previous versions of the article
3.17
identification
establishment of the identity of an organism or virus by next generation sequence (NGS) (3.25) analysis
3.18
insert size
length of the sequence between the sequencing adapters
[10]
[SOURCE: Reference This reference is informative]]
3.19
JavaScript object notation
JSON
open and text-based exchange format
3.20
knowledge representation
process or result of encoding and storing information so that it can be interpreted and used by a computer
3.21
length of longest contig
size of the longest consensus region of DNA produced from a set of overlapping DNA segment reads
3.22
machine readable
data in a format that is easily interpretable by a machine while not necessarily recognizable by humans
3.23
metadata
data that describe other data
3.24
N50
fifty per cent of the entire next generation sequence (NGS) (3.25) assembly that is contained in contigs (3.9) or
scaffolds equal to or larger than this value in base length
Note 1 to entry: Individual sequencing reads are processed to remove linkers and barcodes and then assembled into
overlapping contigs. The next step is to assemble the contigs into progressively larger scaffolds by bridging the gaps
between contigs with additional sequence reads until the entire genome is assembled.
3.25
next generation sequence
NGS
nucleotide sequence (3.27) produced using a massively parallel sequencing methodology
3.26
NG50
resembles N50 (3.24) except the metric relates to the genome size rather than the assembly size
[11]
[SOURCE: Reference This reference is informative]]
3.27
nucleotide sequence
order of purine or pyrimidine nucleosides linked by a phosphodiester backbone and hydrogen bonding in a
deoxyribonucleotide or ribonucleotide molecule
3.28
number of contigs
total count of contiguous DNA sequences after assembly of the whole genome sequence
3.29
number of reads
total quantity of sequenced DNA fragments, or reads, generated during a sequencing run
3.30
OBO Foundry
Open Biological and Biomedical Ontologies Foundry
open biological and biomedical ontology
3.31
ontology
logical structure of the terms used to describe a domain of knowledge, including both the definitions of the
applicable terms and their relationships
3.32
Ontology Web Language
OWL
family of knowledge representation (3.20) or ontology (3.31) languages for authoring ontologies or knowledge
bases
3.33
pod5
specialized nucleotide sequence (3.27) format interconvertible with FASTA, consisting of universally unique
identifiers based on a custom container with the extension
Note 1 to entry: pod 5 is usually used for nanopore based sequencing.
3.34
quantification
determination of the amount of biological material in a sample
3.35
raw read
initial, unaligned data generated by a sequencing instrument
3.36
reifiable
capable of being made more concrete or real
3.37
resource description framework
RDF
extensible markup language (XML) syntax for describing metadata (3.23)
3.38
responsible party
person(s) responsible for the provision of the standard requirements
3.39
semantic interoperability
ability of data shared by systems to be understood at the level of fully defined domain concepts
3.40
sequence length distribution
spread of sequence read sizes in next generation sequence (NGS) (3.25) run
[SOURCE: ]
3.41
surveillance
close observation through microbiological sampling and analysis
3.42
universal character set
UCS
character set encoding standard for international electronic communication
3.43
universal resource identifier
URI
sequence of characters, capable of uniquely identifying the thing with which it is associated, within a specified
context (3.8)
Note 1 to entry: URI is an internet protocol standard that builds on the uniform resource indicator protocol by greatly
expanding the set of permitted characters.
3.44
variant
nucleotide polymorphism, insertion, or deletion found in one or more sequences compared to other sequences
3.45
verified
provision of objective evidence that a given item fulfils specified requirements.
3.46
verified next generation sequence
VNGS
next generation nucleotide sequence that conforms with validation criteria to ensure precision, accuracy,
reliability, repeatability and reproducibility for use in sequence analysis and bioinformatics
3.47
FASTA format
text-based file format and a set of bioinformatics programs used for alignments
4 Abbreviated terms
FHRJSON JavaScript object notationFair bioHeaders Reference Genome Assembly
JSONFHR Fair bioHeader reference genome assemblyJavaScript Object Notation
NGS next generation sequence
OWL Ontology web languageWeb Language
RDF Resourceresource data framework
UCS Universal character setCharacter Set
URI Universal resource identifierResource Identifier
VNGS Verifiedverified next generation sequence
XML extenisble markup languageExtensible Markup Language
5 VNGS requirements
5.1 General
These requirements are not dependent upon a particular NGS platform. Nucleotide sequence and motif
[1312]
identification techniques can vary dependent upon the methods used.
Field Code Changed
5.2 VNGS use case
Nucleotide sequences referred to as verified next generation sequences (VNGS) can be used as reference
sequences as part of method development, verification, and validation in detection, identification, and
quantification for determination, surveillance, diagnosis, genomics and bioinformatics.
6 VNGS identifier scheme
6.1 VNGS URI
The VNGS shall be considered fit for purpose if it possesses a specific namespace and context. The VNGS URI
shall be in the form:
Scheme: //hierarchical namespace qualifier(s)/name
where the ASCII character set is used,.
NOTE In the case of a VNGS IRI, UCS characters are used in place of ASCII characters.
The VNGS URI can be represented in any scheme, e.g. http: urn (www.iana.org). All metadata connected to the
VNGS URI shall be capturable and shall be kept over the whole lifetime of the data. The VNGS URI shall be
persistent and shall remain independent of its mapping on a server, and its notation. The VNGS URI should not
attempt to infer from properties of the NGS data and metadata, including raw data, base quality metadata,
metadata, alignments, variants, features, etc. TheVNGSThe VNGS URI shall identify a single VNGS and each
[13]
VNGS shall be assigned to only one URI. .
Using the same VNGS URI to identify more than one organism or virus is discouraged; e
...
Frequently Asked Questions
ISO/FDIS 25184 is a draft published by the International Organization for Standardization (ISO). Its full title is "Molecular biomarker analysis - Nucleotide sequencing - Verified next generation sequences (VNGS)". This standard covers: This document provides requirements for reference nucleotide sequences. This document applies to all nucleotide sequences determined by next-generation sequencing (NGS) technology that are accessible on the semantic web and included in a genetic database (public or private).
This document provides requirements for reference nucleotide sequences. This document applies to all nucleotide sequences determined by next-generation sequencing (NGS) technology that are accessible on the semantic web and included in a genetic database (public or private).
ISO/FDIS 25184 is classified under the following ICS (International Classification for Standards) categories: 07.080 - Biology. Botany. Zoology. The ICS classification helps identify the subject area and facilitates finding related standards.
You can purchase ISO/FDIS 25184 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of ISO standards.
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