ISO/TS 17307:2016

Petroleum products — Biodiesel — Determination of total esters content by gas chromatography

ISO/TS 17307:2016 establishes a method for determining the total methyl ester content in fatty acid methyl ester (FAME) by gas chromatography and using an external standard. The method is suitable for biodiesel which contains esters between C6 and C26. This method allows verifying that the total ester content is greater than 96,5 % (m/m). NOTE 1 The method also allows determination of the total ethyl ester content in FAEE, but precision for this has not been established. This Technical Specification does not determine the linolenic nor the poly-unsaturated alkyl ester content. Alternative techniques, such as EN 14103[1] and EN 15779[2], respectively, are available for this. NOTE 2 For the purposes of this Technical Specification, the term "% (m/m)" is used to represent the mass fraction, µ.

Produits pétroliers — Biodiesel — Dosage de la teneur en esters totale par chromatographie en phase gazeuse

General Information

• Status: Published
• Publication Date: 18-Jan-2016
• ICS: 75.160.20 - Liquid fuels
• ICS: 75.160.40 - Biofuels
• Technical Committee: ISO/TC 28/SC 7 - Liquid Biofuels
• Drafting Committee: ISO/TC 28/SC 7 - Liquid Biofuels
• Current Stage: 9060 - Close of review
• Start Date: 02-Dec-2025

Standards Content (Sample)

TECHNICAL ISO/TS
SPECIFICATION 17307
First edition
2016-02-01
Petroleum products — Biodiesel —
Determination of total esters content
by gas chromatography
Produits pétroliers — Biodiesel — Dosage de la teneur en esters
totale par chromatographie en phase gazeuse
Reference number
ISO/TS 17307:2016(E)
ISO 2016
---------------------- Page: 1 ----------------------
ISO/TS 17307:2016(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2016, Published in Switzerland

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form

or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior

written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of

the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2016 – All rights reserved
---------------------- Page: 2 ----------------------
ISO/TS 17307:2016(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 1

5 Apparatus ..................................................................................................................................................................................................................... 2

6 Reagents and materials ................................................................................................................................................................................. 2

7 Sampling ........................................................................................................................................................................................................................ 3

8 Procedure..................................................................................................................................................................................................................... 3

8.1 Preparation of the apparatus ...................................................................................................................................................... 3

8.2 Preparation of the calibration curve .................................................................................................................................... 4

8.3 Sample preparation ............................................................................................................................................................................ 4

8.4 Procedure .................................................................................................................................................................................................... 4

9 Expression of results ........................................................................................................................................................................................ 5

10 Precision ....................................................................................................................................................................................................................... 5

10.1 General ........................................................................................................................................................................................................... 5

10.2 Repeatability, r ........................................................................................................................................................................................5

10.3 Reproducibility, R .................................................................................................................................................................................5

11 Test report ................................................................................................................................................................................................................... 6

Annex A (informative) Examples of chromatograms ........................................................................................................................... 7

Annex B (informative) Preparation of standard solutions .........................................................................................................10

Bibliography .............................................................................................................................................................................................................................11

© ISO 2016 – All rights reserved iii
---------------------- Page: 3 ----------------------
ISO/TS 17307:2016(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www.iso.org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation on the meaning of ISO specific terms and expressions related to conformity

assessment, as well as information about ISO’s adherence to the WTO principles in the Technical

Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information

The committee responsible for this document is ISO/TC 28, Petroleum products and related products of

synthetic or biological origin, Subcommittee SC 7, Liquid biofuels.
iv © ISO 2016 – All rights reserved
---------------------- Page: 4 ----------------------
TECHNICAL SPECIFICATION ISO/TS 17307:2016(E)
Petroleum products — Biodiesel — Determination of total
esters content by gas chromatography

WARNING — The use of this Technical Specification might involve the usage of dangerous

materials and equipment. It is the responsibility of the user to establish the appropriate security,

health and environmental practices, and to determine the applicability of regulatory limitations

before their use.
1 Scope

This Technical Specification establishes a method for determining the total methyl ester content in

fatty acid methyl ester (FAME) by gas chromatography and using an external standard. The method is

suitable for biodiesel which contains esters between C6 and C26. This method allows verifying that the

total ester content is greater than 96,5 % (m/m).

NOTE 1 The method also allows determination of the total ethyl ester content in FAEE, but precision for this

has not been established.

This Technical Specification does not determine the linolenic nor the poly-unsaturated alkyl ester

[1] [2]

content. Alternative techniques, such as EN 14103 and EN 15779 , respectively, are available for this.

NOTE 2 For the purposes of this Technical Specification, the term “% (m/m)” is used to represent the mass

fraction, µ.
2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated

references, the latest edition of the referenced document (including any amendments) applies.

ISO 3170, Petroleum liquids — Manual sampling
ISO 3171, Petroleum liquids — Automatic pipeline sampling

ISO 4259, Petroleum products — Determination and application of precision data in relation to

methods of test
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
biodiesel

fuel comprised of monoalkyl esters of fatty acids, derived from vegetable oils or animal fat

3.2
total esters
sum of concentration of all esters (C6 – C26)
4 Principle

A sample is analysed by gas chromatography using an external calibration method to quantify the

esters present in biodiesel regardless of the raw material used in its production.

© ISO 2016 – All rights reserved 1
---------------------- Page: 5 ----------------------
ISO/TS 17307:2016(E)

The quantification of biodiesel samples with C18 carbon chains is performed using an analytical curve

prepared with methyl or ethyl octadecanoate. If there is presence of ester
...

TECHNICAL ISO/TS
SPECIFICATION 17307
First edition
Petroleum products — Biodiesel —
Determination of total esters content
by gas chromatography
Produits pétroliers — Biodiesel — Dosage de la teneur en esters
totale par chromatographie en phase gazeuse
PROOF/ÉPREUVE
Reference number
ISO/TS 17307:2015(E)
ISO 2015
---------------------- Page: 1 ----------------------
ISO/TS 17307:2015(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2015, Published in Switzerland

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form

or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior

written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of

the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2015 – All rights reserved
---------------------- Page: 2 ----------------------
ISO/TS 17307:2015(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 1

5 Apparatus ..................................................................................................................................................................................................................... 2

6 Reagents and materials ................................................................................................................................................................................. 2

7 Sampling ........................................................................................................................................................................................................................ 3

8 Procedure..................................................................................................................................................................................................................... 3

8.1 Preparation of the apparatus ...................................................................................................................................................... 3

8.2 Preparation of the calibration curve .................................................................................................................................... 4

8.3 Sample preparation ............................................................................................................................................................................ 4

8.4 Procedure .................................................................................................................................................................................................... 4

9 Expression of results ........................................................................................................................................................................................ 5

10 Precision ....................................................................................................................................................................................................................... 5

10.1 General ........................................................................................................................................................................................................... 5

10.2 Repeatability, r ........................................................................................................................................................................................5

10.3 Reproducibility, R .................................................................................................................................................................................5

11 Test report ................................................................................................................................................................................................................... 6

Annex A (informative) Examples of chromatograms ........................................................................................................................... 7

Annex B (informative) Preparation of standard solutions .........................................................................................................10

Bibliography .............................................................................................................................................................................................................................11

© ISO 2015 – All rights reserved PROOF/ÉPREUVE iii
---------------------- Page: 3 ----------------------
ISO/TS 17307:2015(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www.iso.org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation on the meaning of ISO specific terms and expressions related to conformity

assessment, as well as information about ISO’s adherence to the WTO principles in the Technical

Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information

The committee responsible for this document is ISO/TC 28, Petroleum products and related products of

synthetic or biological origin, Subcommittee SC 7, Liquid biofuels.
iv PROOF/ÉPREUVE © ISO 2015 – All rights reserved
---------------------- Page: 4 ----------------------
TECHNICAL SPECIFICATION ISO/TS 17307:2015(E)
Petroleum products — Biodiesel — Determination of total
esters content by gas chromatography

WARNING — The use of this Technical Specification might involve the usage of dangerous

materials and equipment. It is the responsibility of the user to establish the appropriate security,

health and environmental practices, and to determine the applicability of regulatory limitations

before their use.
1 Scope

This Technical Specification establishes a method for determining the total methyl ester content in

fatty acid methyl ester (FAME) by gas chromatography and using an external standard. The method is

suitable for biodiesel which contains esters between C6 and C26. This method allows verifying that the

total ester content is greater than 96,5 % (m/m).

NOTE 1 The method also allows determination of the total ethyl ester content in FAEE, but precision for this

has not been established.

This Technical Specification does not determine the linolenic nor the poly-unsaturated alkyl ester

[1] [2]

content. Alternative techniques, such as EN 14103 and EN 15779 , respectively, are available for this.

NOTE 2 For the purposes of this Technical Specification, the term “% (m/m)” is used to represent the mass

fraction, µ.
2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated

references, the latest edition of the referenced document (including any amendments) applies.

ISO 3170, Petroleum liquids — Manual sampling
ISO 3171, Petroleum liquids — Automatic pipeline sampling

ISO 4259, Petroleum products — Determination and application of precision data in relation to

methods of test
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
biodiesel

fuel comprised of monoalkyl esters of fatty acids, derived from vegetable oils or animal fat

3.2
total esters
sum of concentration of all esters (C6 – C26)
4 Principle

A sample is analysed by gas chromatography using an external calibration method to quantify the

esters present in biodiesel regardless of the raw material used in its production.

© ISO 2015 – All rights reserved PROOF/ÉPREUVE 1
---------------------- Page: 5 ----------------------
ISO/TS 17307:2015(E)

The quantification of biodiesel samples with C18 carbon chains is performed using an analytical curve

prepared with methyl or ethyl octadecanoate. If there is presence of esters with carbon chain shorter

than C , in addition m
...