ISO 22195-6:2021

Textiles - Determination of index ingredient from coloured textile - Part 6: Punica granatum

This document specifies a test method which identifies the index ingredient chemical included in coloured fabric with punica. Punica can be applied to both natural fibre and man-made fibre.

Textiles — Détermination d'indicateurs d'ingrédients de textiles colorés — Partie 6: Grenadier

General Information

• Status: Published
• Publication Date: 08-Nov-2021
• ICS: 59.080.01 - Textiles in general
• Technical Committee: ISO/TC 38 - Textiles
• Drafting Committee: ISO/TC 38/WG 31 - Natural material for textiles
• Current Stage: 6060 - International Standard published
• Start Date: 09-Nov-2021
• Due Date: 12-Jul-2021
• Completion Date: 09-Nov-2021

Overview

ISO 22195-6:2021 is an international standard that specifies a test method for identifying the index ingredient chemical derived from Punica granatum (pomegranate) used in coloured textiles. This standard is part 6 of the ISO 22195 series focusing on the determination of natural dye components in textiles. It applies to both natural fibres and man-made fibres dyed with punica extracts.

Developed by ISO Technical Committee ISO/TC 38 on Textiles, the method uses chromatographic analysis to detect punicalagin, the characteristic chemical marker for punica dye. The standard supports verification of natural dye claims often associated with environmental benefits, ensuring accurate identification of punica-dyed fabrics in the textile industry.

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Key Topics

• Scope and Application
  The standard applies to dyed textiles where Punica granatum extract is used as a natural colourant. It allows identification of the punicalagin compound in both natural and synthetic fibres.

• Test Methodology
  The method involves preparing fabric samples and extracting dissolved colourant using trifluoroacetic acid. Extraction is performed via an ultrasonic bath followed by filtration. The solution is then analyzed with High-Performance Liquid Chromatography coupled with a Photo Diode Array detector (HPLC-PDA).

• Chemical Marker – Punicalagin
  Punicalagin is identified by its retention time and absorbance spectrum (wavelength 260 nm) during chromatographic analysis. The detection confirms the presence of punica-derived dye in the textile sample.

• Reagents and Equipment
  Analytical grade reagents such as trifluoroacetic acid, acetonitrile, and distilled water are used. Specialized apparatus includes ultrasonic water bath, HPLC systems with PDA detectors, and membrane filters ensuring precise preparation and analysis.

• Test Reporting
  The standard details essential information to be included in reports: sample identification, chromatographic conditions, results, and any procedural deviations. This ensures consistent documentation for quality assurance and regulatory compliance.

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Applications

• Textile Quality Assurance
  ISO 22195-6:2021 assists textile manufacturers and testing laboratories in verifying whether fabrics were dyed with pomegranate-based natural dyes, supporting product authenticity claims.

• Environmental Impact Verification
  As natural dyes are valued for their eco-friendly profiles, this method enables brands and certification bodies to substantiate environmental claims linked to natural dye usage.

• Research and Development
  Researchers can apply the standard to study dye uptake on various fibre types or to develop new sustainable dyeing processes using punica extracts.

• Customs and Regulatory Compliance
  Customs agencies and regulatory authorities can use this standard to validate import/export claims about textile composition and dyeing methods.

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Related Standards

• ISO 22195 Series
  A broader set of standards for determining various natural dye ingredients in coloured textiles beyond punica, covering other plant-based dyes.

• ISO 3696:2018
  Specifies water quality requirements for laboratory use, referenced here for reagent preparation to ensure analytical accuracy.

• ISO/IEC Directives, Part 2
  Provides editorial rules for preparing and maintaining ISO standards such as ISO 22195-6:2021.

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By implementing ISO 22195-6:2021, textile professionals gain a scientifically validated approach for natural dye identification. This aids in maintaining transparency, quality, and ecological responsibility in the textile supply chain, aligning with growing consumer demand for sustainable and authentic textile products.

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 22195-6
First edition
2021-11
Textiles — Determination of index
ingredient from coloured textile —
Part 6:
Punica granatum
Textiles — Détermination d'indicateurs d'ingrédients de textiles
colorés —
Partie 6: Grenadier
Reference number
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 2
7 Procedure .2
7.1 Standard preparation . . 2
7.2 Preparation of specimen . . 2
7.3 Analysis . 3
7.4 Qualification of punicalagin. 3
8 Test report . 3
Annex A (informative) Example of test result . 4
iii
Foreword
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This document was prepared by Technical Committee ISO/TC 38, Textiles.
A list of all parts in the ISO 22195 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
There is no doubt that dyeing plays the most important role in expressing the colour of clothes. Until
the invention of synthetic dyes capable of expressing diverse colours today, humankind used materials
obtained from nature to dye fabric. Typically, colourants were obtained from plants or various materials
were extracted from minerals or insects. Dyeing fabrics using materials derived from these natural
substances made it necessary to identify which substances the colourant was derived from. In other
words, there has been a demand to confirm whether a fabric has been dyed with a natural substance.
There are several natural dyes raw material which give similar colour tone, they have different colouring
molecule and the precise colorant. But each has different environmental profile whi
...