ISO 17299-6:2025

Textiles - Determination of deodorant property - Part 6: Gas chromatography method using automated dosing and sampling

This document specifies gas chromatography (GC) test methods for the deodorant testing of all textile products. These methods apply to odour component chemicals, such as indole, isovaleric acid and nonenal. Two methods are described in this document. - Method A (single analysis) where an odour chemical is applied in a container avoiding contact with a test specimen. - Method B (auto-regeneration) where an odour chemical is applied in a container avoiding contact with a test specimen multiple times to repeatedly challenge a test specimen.

Textiles — Détermination des propriétés de neutralisation d'odeurs — Partie 6: Méthode par chromatographie en phase gazeuse utilisant un dosage et un échantillonnage automatisés

General Information

• Status: Published
• Publication Date: 21-May-2025
• ICS: 59.080.01 - Textiles in general
• Technical Committee: ISO/TC 38 - Textiles
• Drafting Committee: ISO/TC 38 - Textiles
• Current Stage: 6060 - International Standard published
• Start Date: 22-May-2025
• Due Date: 16-Dec-2025
• Completion Date: 22-May-2025

Overview

ISO 17299-6:2025 - Textiles - Determination of deodorant property - Part 6 specifies a gas chromatography (GC) method using automated dosing and sampling to measure the deodorant performance of textile products. Published as the first edition in 2025, the standard describes headspace GC measurement of representative odour chemicals (indole, isovaleric acid, 2-nonenal) to calculate an odour reduction rate for treated or untreated textiles. The procedure is intended for laboratories equipped with GC (FID or MS detector) and an auto-sampling/auto-dosing headspace system.

Key topics and technical requirements

• Scope: GC test methods for deodorant testing of all textile products using individual odour component chemicals (indole, isovaleric acid, nonenal).
• Two test methods:
  • Method A (single analysis) - one-time dosing into vials avoiding direct liquid contact with specimens.
  • Method B (auto-regeneration) - repeated automated dosing to simulate multiple odour challenges.
• Instrumentation: GC with FID or MSD, 20 ml glass injection vials with PTFE/silicone septa, and an auto-sampling device with headspace injection and liquid dosing capability. Auto-sampler requirements include heating/agitation up to (60 ± 2) °C for 60 min and agitation ~400 r/min.
• Test environment: Conditioning and testing at (20 ± 2) °C and (65 ± 4) % RH in accordance with ISO 139.
• Specimen preparation: Fabric specimens 13 cm × 13 cm (±0.1 cm) or yarn/fibre samples 0.125 g (±0.005 g); three specimens per chemical test and controls without specimen.
• Reagents and solutions: Analytical-grade indole, isovaleric acid and nonenal prepared in ethanol at specified concentrations (shelf-life ~6 months).
• Procedure highlights: vial conditioning (oven 105 °C for 15 min), automated dosing, headspace sampling, GC analysis, peak area comparison between vials with and without specimen, and calculation of odour reduction rate.
• Documentation: test report requirements and example reports provided; GC parameters and method-selection guidance included in informative annexes.

Applications

• Performance testing of deodorant finishes and treated textiles (apparel, bedding, technical textiles).
• Comparative R&D and product optimization to quantify odour-capture or odour-neutralizing efficacy.
• Quality control and validation for manufacturers and independent testing laboratories.
• Supporting product claims, procurement specifications, and regulatory or certification evidence.

Who should use this standard

• Textile R&D teams and formulation chemists developing deodorant treatments.
• Accredited testing laboratories performing textile odour performance tests.
• Manufacturers, brand owners, and procurement specialists requiring objective deodorant performance data.

Related standards

• ISO 17299 series (other parts on deodorant testing methods).
• ISO 17299-3 (manual dosing and sampling method) - useful for direct comparison of automated vs manual procedures.
• ISO 139 (conditioning and testing atmosphere) and ISO 3696 (laboratory water quality) - normative references cited by ISO 17299-6.

Keywords: ISO 17299-6, textile deodorant testing, gas chromatography, automated dosing, headspace sampling, odour reduction rate, indole, isovaleric acid, 2-nonenal, GC-FID, GC-MS.

Standards Content (Sample)

International
Standard
ISO 17299-6
First edition
Textiles — Determination of
2025-05
deodorant property —
Part 6:
Gas chromatography method using
automated dosing and sampling
Textiles — Détermination des propriétés de neutralisation
d'odeurs —
Partie 6: Méthode par chromatographie en phase gazeuse
utilisant un dosage et un échantillonnage automatisés
Reference number
© ISO 2025
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Reagents and materials . 2
5 Apparatus . 2
6 Preparation and preservation of test samples and test specimens . 3
7 Procedure . 4
7.1 General .4
7.2 Method A — Single analysis procedure .4
7.2.1 Preparation of injection vials . .4
7.2.2 Placement of test specimen . .4
7.2.3 Placement of injection vials .4
7.2.4 Conditioning the test specimens .4
7.2.5 Preparation of the odour component chemical solution .4
7.2.6 Injection of testing odour component chemical solution .5
7.2.7 Sampling of the testing gas .5
7.2.8 Concentration measurement of testing gas by GC .5
7.2.9 Peak area of MS/FID spectrum of testing gas with test specimen .5
7.2.10 Concentration of testing gas without specimen .5
7.3 Method B — Auto-regeneration procedure .5
7.3.1 Analysis .5
7.3.2 Repeat cycles .5
8 Calculation of odour reduction rate . 6
9 Test report . 6
Annex A (informative) GC parameters . 7
Annex B (informative) Selection of the test condition comparing to ISO 17299-3 . 9
Annex C (informative) Example of test result . 10
Annex D (informative) Example of odour reduction rate test report .13
Bibliography . 14

iii
Foreword
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The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
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Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 38, Textiles.
A list of all parts in the ISO 17299 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
Introduction
This document describes a gas chromatography testing method for concentration measurement of odour
chemicals in deodorant testing. This is the most general testing method that can be carried out in many
testing laboratories globally if they have gas chromatography equipped with accessories for automated
dosing and sampling.
This document describes a specific procedure for the gas chromatography testing method using automated
dosing and sampling, while ISO 17299-3 is based on manual dosing and sampling.
Concentrations of the gaseous odour component chemicals in containers with or without a test specimen
after a designated contact time are measured by GC. The reduction rate of concentration of odour chemicals
in the container with a test specimen is calculated from the concentration data with a test specimen and
without a test specimen.
Each chemical odorant is tested separately. Odour chemical contact with fabric can be uncontrollable due to
auto-dosing device. However, heating and agitation are expected to volatilize the odorant solution.

v
International Standard ISO 17299-6:2025(en)
Textiles — Determination of deodorant property —
Part 6:
Gas chromatography method using automated dosing and
sampling
1 Scope
This document specifies gas chromatography (GC) test methods for the deodorant testing of all textile
products. These methods apply to odour component chemicals, such as indole, isovaleric acid and nonenal.
Two methods are described in this document.
— Method A (single analysis) where an odour chemical is applied in a container avoiding contact with a test
specimen.
— Method B (auto-regeneration) where an odour chemical is applied in a container avoiding contact with a
test specimen multiple times to repeatedly challenge a test specimen.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 139, Textiles — Standard atmospheres for conditioning and testing
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org
3.1
test specimen
test piece that is cut from a sample of a textile product (3.3), as specified by the test procedure, or a
representative sample of the product itself
3.2
textile deodorant
textile with the ability to reduce the unpleasant odour (3.4) in the ambient air around textile products (3.3)
Note 1 to entry: The unpleasant odour is caused by the chemicals listed in 4.1, 4.2 and 4.3.
[SOURCE: ISO 17299-1:2014, 3.1.4, modified — cross reference changed in Note 1]

3.3
textile product
product made of textile fibres, yarns and/or fabrics and intended to be used, as such or in conjunction with
other textile or non-textile elements
3.4
unpleasant odour
uncomfortable odour related to the human living environment, such as toilet odour, sweat odour, body odour
(nonenal mixture odour), excrement odour, etc.
Note 1 to entry: These chemicals are as follows:
— sweat odour: isovaleric acid (4.2);
— body odour: isovaleric acid (4.2) and nonenal (4.3);
— excrement odour: indole (4.1).
Note 2 to entry: Other chemicals might be relevant. The selected chemicals are conceived as representative.
[SOURCE: ISO 17299-1:2014, 3.1.1, modified — Notes to entry have been added.]
3.5
auto-sampling device
device used to automatically and reproducibly deliver the odour chemicals (3.4) into the injection vials and
uptake the headspace sample that is to be analysed
3.6
auto-regeneration
ability of a textile deodorant to continue odour capture actions when odour chemical application is repeated
4 Reagents and materials
Unless otherwise specified, analytical grades shall be used.
1)
4.1 Indole (C H N), (CAS RN® 120-72-9) reagent.
8 7
1)
4.2 Isovaleric acid, (CAS RN® 503-74-2) reagent with a purity of 98 %.
1)
4.3 2-Nonenal (C H O), (CAS RN® 18829-56-6) reagent with a purity of 95,0 %.
9 16
1)
4.4 Methanol, (CAS RN® 67-56-1) for cleaning scissors/rotary cutter.
1)
4.5 Ethanol, (CAS RN® 64-17-5) reagent grade.
4.6 Water, grade 3 as specified in ISO 3696.
5 Apparatus
The usual laboratory apparatus and, in particular, the following shall be used.
5.1 Analytical balance, capable of weighing accurately to 0,000 1 g.
1) CAS Registry Number® is a trademark of the American Chemical Society (ACS). This information is given for the
convenience of users of this document and does not constitute an endorsement by ISO of the product named. Equivalent
products may be used if they can be shown to lead to the same results.

5.2 Injection vial, with a capacity of 20 ml, made of glass, screw neck with 18 mm screw cap with PTFE/
silicone septum.
5.3 Injection vial sample metal rack.
5.4 Cutting tools, for example scissors/rotary cutter/die cutter that cut textile products.
5.5 Low-lint tissues, for cleaning scissors/rotary cutter.
5.6 Template, 3,6 cm × 3,6 cm.
5.7 Beaker, with a capacity of 250 ml.
5.8 Volumetric flask, with a capacity of 100 ml, class A.
5.9 Glassware drying oven.
5.10 Gas chromatography (GC) apparatus, with configurations, with a flame ionization detector (FID) or
mass selective detector (MSD) (see Annex A for GC parameters).
5.11 Auto-Sampling device, with both the headspace injection syringe and liquid dosing syringe capability
with heating agitator capable of heating up to (60 ± 2) °C for 60 min and agitator speed of 400 r/min.
6 Preparation and preservation of test samples and test specimens
6.1 The testing environment used in
...