Durability of wood and wood-based products - Test method against wood destroying basidiomycetes - Part 1: Assessment of biocidal efficacy of wood preservatives

This document specifies a method for determining the efficacy of wood preservatives applied to wood by
penetration treatment against wood destroying basidiomycetes cultured on a malt extract agar medium.
The method is applicable to formulated products or to their active ingredients.
NOTE This method can be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
Annex A (informative) contains an example of a test report.
Annex B (informative) contains some methods of sterilization.
Annex C (informative) contains information on the test vessels.
Annex D (informative) contains information on test fungi.
Annex E (informative) contains a recommended but non-comprehensive list of optional fungi.

Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren gegen Holz zerstörende Basidiomyceten - Teil 1: Bewertung der bioziden Wirksamkeit von Holzschutzmitteln

Dieses Dokument legt ein Verfahren zur Bestimmung der Wirksamkeit von Holzschutzmitteln gegen Holz zerstörende, auf Agarnährboden gezüchtete Basidiomyceten fest, nachdem die Mittel durch ein Tränkverfahren in das Holz eingebracht wurden.
Dieses Verfahren ist auf formulierte Produkte oder deren Wirkstoffe anwendbar.
ANMERKUNG Dieses Verfahren kann in Verbindung mit einer Alterungsbeanspruchung, z. B. EN 73 oder EN 84, angewendet werden.
Anhang A (informativ) enthält ein Beispiel für einen Prüfbericht.
Anhang B (informativ) enthält einige Sterilisationsverfahren.
Anhang C (informativ) enthält Informationen zu den Prüfgefäßen.
Anhang D (informativ) enthält Informationen zu Prüfpilzen.
Anhang E (informativ) enthält eine empfohlene unvollständige Liste von fakultativenPrüfpilzen.

Durabilité du bois et des matériaux dérivés du bois - Méthode d'essai vis-à-vis des champignons basidiomycètes - Partie 1 : Détermination de l'efficacité protectrice de produits de préservation

Le présent document décrit une méthode de détermination de l'efficacité d'un produit de préservation du bois, appliqué par traitement par imprégnation profonde, vis-à-vis des champignons basidiomycètes lignivores cultivés sur milieu malt-agar.
La méthode est applicable aux produits formulés ou à leurs matières actives.
NOTE Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple selon l'EN 73 ou l'EN 84.
L'Annexe A (informative) donne un exemple de rapport d'essai.
L'Annexe B (informative) donne plusieurs méthodes de stérilisation.
L'Annexe C (informative) donne des informations sur les flacons d'essai.
L'Annexe D (informative) donne des informations sur les champignons d'essai.
L'Annexe E (informative) donne une liste recommandée mais non exhaustive de champignons facultatifs.

Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam prostotrosnicam - 1. del: Ocenjevanje biocidne učinkovitosti biocidnih proizvodov za les

General Information

Status
Published
Public Enquiry End Date
19-Dec-2018
Publication Date
26-Jan-2021
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
11-Jan-2021
Due Date
18-Mar-2021
Completion Date
27-Jan-2021

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SLOVENSKI STANDARD
SIST EN 113-1:2021
01-marec-2021
Nadomešča:
SIST EN 113:2002
SIST EN 113:2002/A1:2004
Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam

prostotrosnicam - 1. del: Ocenjevanje biocidne učinkovitosti biocidnih proizvodov

za les
Durability of wood and wood-based products - Test method against wood destroying
basidiomycetes - Part 1: Assessment of biocidal efficacy of wood preservatives

Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren gegen Holz zerstörende

Basidiomyceten - Teil 1: Bewertung der bioziden Wirksamkeit von Holzschutzmitteln

Durabilité du bois et des matériaux dérivés du bois - Méthode d'essai vis-à-vis des

champignons basidiomycètes - Partie 1 : Détermination de l'efficacité protectrice de

produits de préservation
Ta slovenski standard je istoveten z: EN 113-1:2020
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
SIST EN 113-1:2021 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 113-1:2021
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SIST EN 113-1:2021
EN 113-1
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2020
EUROPÄISCHE NORM
ICS 71.100.50 Supersedes EN 113:1996,
EN 113:1996/A1:2004
English Version
Durability of wood and wood-based products - Test
method against wood destroying basidiomycetes - Part 1:
Assessment of biocidal efficacy of wood preservatives

Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -

Méthode d'essai vis-à-vis des champignons Prüfverfahren gegen Holz zerstörende Basidiomyceten

basidiomycètes - Partie 1 : Détermination de l'efficacité - Teil 1: Bewertung der bioziden Wirksamkeit von

protectrice de produits de préservation Holzschutzmitteln
This European Standard was approved by CEN on 2 November 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 113-1:2020 E

worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
SIST EN 113-1:2021
EN 113-1:2020 (E)
Contents Page

European foreword ....................................................................................................................................................... 4

Introduction .................................................................................................................................................................... 5

1 Scope .................................................................................................................................................................... 6

2 Normative references .................................................................................................................................... 6

3 Terms and definitions ................................................................................................................................... 6

4 Principles ........................................................................................................................................................... 6

5 Test material and apparatus ....................................................................................................................... 6

5.1 Biological material .......................................................................................................................................... 6

5.1.1 General ................................................................................................................................................................ 6

5.1.2 Obligatory fungi in all cases (see also Annex D) .................................................................................. 6

5.1.3 Obligatory fungus for particular uses (see also Annex D) ................................................................ 7

5.1.4 Maintenance of strains .................................................................................................................................. 7

5.2 Products and reagents ................................................................................................................................... 7

5.2.1 Culture medium ............................................................................................................................................... 7

5.2.2 Solvents and diluents ..................................................................................................................................... 8

5.3 Apparatus ........................................................................................................................................................... 8

5.3.1 Conditioning chamber ................................................................................................................................... 8

5.3.2 Culture chamber .............................................................................................................................................. 8

5.3.3 Drying oven ....................................................................................................................................................... 8

5.3.4 Treatment vessels ........................................................................................................................................... 8

5.3.5 Ballast .................................................................................................................................................................. 8

5.3.6 Safety equipment and protective clothing ............................................................................................. 8

5.3.7 Vacuum vessels ................................................................................................................................................ 9

5.3.8 Vacuum pump ................................................................................................................................................... 9

5.3.9 Kolle flasks or equivalent culture vessels .............................................................................................. 9

5.3.10 Test specimen supports ................................................................................................................................ 9

5.3.11 Drying vessel(s) ............................................................................................................................................... 9

5.3.12 Equipment for steam sterilization or access to a radiation source .............................................. 9

5.3.13 Ordinary laboratory equipment ................................................................................................................ 9

6 Sampling of the preservative ...................................................................................................................... 9

7 Test specimens ................................................................................................................................................. 9

7.1 Wood species .................................................................................................................................................... 9

7.2 Wood quality .................................................................................................................................................. 10

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SIST EN 113-1:2021
EN 113-1:2020 (E)

7.3 Provision of test specimens ...................................................................................................................... 10

7.4 Dimensions and density of test specimens.......................................................................................... 10

7.5 Number and distribution of test specimens........................................................................................ 10

8 Procedure ........................................................................................................................................................ 11

8.1 Conditioning of test specimens before treatment ............................................................................ 11

8.2 Treatment of test specimens .................................................................................................................... 11

8.2.1 Preparation of treatment solutions/dilutions ................................................................................... 11

8.2.2 Impregnation ................................................................................................................................................. 12

8.3 Drying and conditioning of test specimens after treatment ......................................................... 12

8.4 Exposure to fungi .......................................................................................................................................... 13

8.5 Culture conditions and duration of test ............................................................................................... 13

8.6 Assessment of test ........................................................................................................................................ 14

8.6.1 Examination of the test specimens ......................................................................................................... 14

8.6.2 Loss in mass caused by fungal attack .................................................................................................... 14

8.6.3 Validity of results .......................................................................................................................................... 14

8.6.4 Assessment of results .................................................................................................................................. 15

9 Statement of results ..................................................................................................................................... 15

10 Test report ...................................................................................................................................................... 15

Annex A (informative) Example of a test report ............................................................................................. 17

Annex B (normative) Methods of sterilization ............................................................................................... 23

Annex C (informative) Culture vessels ............................................................................................................... 24

Annex D (informative) Test fungi ........................................................................................................................ 27

Annex E (informative) Non-comprehensive list of optional fungi ........................................................... 29

Bibliography ................................................................................................................................................................. 31

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SIST EN 113-1:2021
EN 113-1:2020 (E)
European foreword

This document (EN 113-1:2020) has been prepared by Technical Committee CEN/TC 38 “Durability of

wood and wood-based products”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by June 2021, and conflicting national standards shall be

withdrawn at the latest by June 2021.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN 113:1996 and EN 113:1996/A1:2004.
Test results obtained with earlier versions of EN 113 are still valid.

Compared to EN 113:1996 and EN 113:1996/A1:2004 the following major changes have been

introduced:

— This is now a first part of EN 113 corresponding to the EN 113:1996 document. Other parts relate

to a different scope.
— The title is changed;
— The obligatory fungi are indicated differently;
— The calculation of a correction factor (C) has been differently included;
— The methods for sterilization are updated;
— All annexes are informative except Annex B;
— Some additional validity requirements are introduced for control specimens.

According to the CEN-CENELEC Internal Regulations, the national standards organisations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,

Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,

Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North

Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United

Kingdom.
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SIST EN 113-1:2021
EN 113-1:2020 (E)
Introduction

This document describes a laboratory method of test, which gives a basis for the assessment of

effectiveness of a wood preservative against wood destroying basidiomycetes. By using this method it is

possible to determine the loading at which impregnated wood of a susceptible species can regarded as

adequately protected under the conditions of test.

This laboratory method provides one criterion by which the efficacy of a product can be assessed, and

this criterion should be used to judge the likely effectiveness of the preservative taking into account the

methods of application likely to be used.

The procedures described in this standard method are intended to be carried out by suitably trained

and/or supervised specialists. Appropriate safety precautions should be observed throughout the use of

the document.
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SIST EN 113-1:2021
EN 113-1:2020 (E)
1 Scope

This document specifies a method for determining the efficacy of wood preservatives applied to wood by

penetration treatment against wood destroying basidiomycetes cultured on a malt extract agar medium.

The method is applicable to formulated products or to their active ingredients.

NOTE This method can be used in conjunction with an ageing procedure, for example EN 73 or EN 84.

Annex A (informative) contains an example of a test report.
Annex B (normative) contains some methods of sterilization.
Annex C (informative) contains information on the test vessels.
Annex D (informative) contains information on test fungi.

Annex E (informative) contains a recommended but non-comprehensive list of optional fungi.

2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
supplier
sponsor of a biological test of a wood preservative
4 Principles

Test specimens of a susceptible wood species impregnated with increasing concentrations of wood

preservative solutions and reference wood test specimens are exposed to attack by pure cultures of

basidiomycetes. After a prescribed period of incubation under defined conditions, the percentage loss in

dry mass of the test specimens is used to establish the biocidal efficacy of the product under test.

5 Test material and apparatus
5.1 Biological material
5.1.1 General
The test fungi to be used as follows:
5.1.2 Obligatory fungi in all cases (see also Annex D)
— Coniophora puteana (Schumach.) P. Karst (BAM Ebw. 15) on softwood.

Loss in mass in percentage in 16 weeks of Scots pine sapwood specimens: a mass fraction of a minimum

20 %.
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SIST EN 113-1:2021
EN 113-1:2020 (E)

— Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel (FPRL 280) on softwood.

Loss in mass in percentage in 16 weeks of Scots pine sapwood specimens: a mass fraction of a minimum

20 %.
— Gloeophyllum trabeum (Persoon) Murrill (BAM Ebw. 109) on softwood.

Loss in mass in percentage in 16 weeks of Scots pine sapwood specimens: a mass fraction of a minimum

20 %.

For testing efficacy against brown rot, only the use of treated softwood is required. The results of testing

treated softwoods against brown rot fungi are valid for hardwoods: additional testing of treated

hardwoods is not required.
5.1.3 Obligatory fungus for particular uses (see also Annex D)

— Trametes versicolor (L.) Lloyd (CTB 863 A) on hardwood and/or on softwood, as appropriate.

Loss in mass in percentage in 16 weeks of beech specimens: a mass fraction of a minimum 20 %; of Scots

pine sapwood specimens: a mass fraction of a minimum 15 %.

NOTE 1 Beech results could be used for softwoods if this is considered a tougher test (see EN 599-1).

NOTE 2 For specific regional uses or conditions, it is also possible to select other fungi on an optional basis .

5.1.4 Maintenance of strains

The strains shall be maintained and treated so that its virulence is conserved and ensured.

NOTE 1 For suggestions on maintenance options see Annex D.

The strains shall be maintained and treated (frequency of subculturing, alternation of culture media, etc.)

in accordance with the instructions of their laboratory of origin (see D.2).

NOTE 2 The parent strain is maintained in the laboratory of its origin so as to conserve and to ensure its vigour.

If tests are not undertaken regularly or if a strain shows signs of degeneration a new standard culture of

the strain should be obtained from the laboratory of its origin for each test (see D.2).

When new strains are received, the virulence shall be tested to ensure the strain can achieve the

minimum loss in mass (see 5.1.2 and 5.1.3).
5.2 Products and reagents
5.2.1 Culture medium
The culture medium is a malt agar medium with the following composition:

— malt extract; in concentrated form: (50 ± 0,5) g; in powder form: (40 ± 0,5) g;

— agar causing no inhibition of growth of fungi: (20 ± 0,5) g to (30 ± 0,5) g;

Former name: Poria placenta (Fries) Cooke sensu J. Eriksson [Synonym: Poria monticola Murrill]

Former name: Coriolus versicolor (Linnaeus) Quelet
See Annex E for a non-comprehensive list of optional fungi.
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SIST EN 113-1:2021
EN 113-1:2020 (E)

— de-ionized water; quantity to make up to 1000 g. Preferably use water conforming to grade 3 of

ISO 3696.

Prepare this medium by warming the mixture in a boiling water bath or a steam bath, stirring until

completely dissolved.

Place in each culture vessel a sufficient quantity of the medium to provide a minimum depth of 3 mm to

4 mm when in its in-use position. Close the vessels as specified in 5.3.9 and sterilize in the autoclave at

121°C for 20 min. Let the vessels cool in their in-use position.
5.2.2 Solvents and diluents
For water soluble preservatives:
— de-ionized water (see 5.2.1).
For preservatives to be diluted or dissolved in an organic solvent:

— suitably volatile liquids, that leave no residue in the wood having a toxic effect on the fungi at the end

of the post-treatment conditioning period.
NOTE Toluene and xylene of recognized analytical grade have been found suitable.
For oil-based preservatives:

— where the carrier oil remains in the wood after treatment and conditioning, the content of carrier oil

in the product should be kept at the level intended to be used in practice. Achieving a range of

retentions according to this document could require the dilution of the product to different

concentrations. Addition of additional carrier oil, which remains in the wood after conditioning,

should not be used as a method to adjust concentration of the product. In this case, a suitably volatile

solvent should be selected.
5.3 Apparatus
5.3.1 Conditioning chamber
Well ventilated and maintained at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.2 Culture chamber

Incubator or room, dark and maintained at (22 ± 2) °C and (70 ± 5) % relative humidity.

5.3.3 Drying oven
Maintained at (103 ± 2) °C.
5.3.4 Treatment vessels
Of a material that does not react with their contents.
5.3.5 Ballast

To prevent floating of test specimens. The ballast shall not react with any materials with which they come

into contact during the test.
5.3.6 Safety equipment and protective clothing

Appropriate for the test product and the test solvents, to ensure the safety of the operator.

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SIST EN 113-1:2021
EN 113-1:2020 (E)
5.3.7 Vacuum vessels
Fitted with stopcocks.
5.3.8 Vacuum pump

Fitted with a pressure gauge and capable of attaining a pressure of (0,7 ± 0,1) kPa.

5.3.9 Kolle flasks or equivalent culture vessels

With a capacity of between 400 ml and 650 ml, providing a flat surface area of between 85 cm and

120 cm for the medium (see Figures C.1, C.2 and C.3 in Annex C) and close with a material that allows

for air exchange.

NOTE Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with

leakproof lids, the centres of which are pierced with a round hole of typically 15 mm diameter and plugged with a

wad of cotton wool.
5.3.10 Test specimen supports

Made of glass, stainless steel or any other inert material, with no risk of having any effect on the culture

medium, the fungus, the wood or the impregnated product, or of being itself modified. The supports are

used to prevent direct contact of the specimens with the culture medium, but shall not separate them

from it by more than 3 mm.

If abnormally high moisture contents (see 8.6.3) are experienced consistently, use of specimen supports

of approximately 5 mm thick could help to control the problem. If thicker specimen supports are used,

this should be recorded in the test report.
5.3.11 Drying vessel(s)

Provided with a close-fitting cover and containing supports that will give minimum contact with the

treated test specimens to be placed on them. The vessels and supports shall be of materials that do not

react with the test solvent or test preservative.
5.3.12 Equipment for steam sterilization or access to a radiation source
See Annex B.
5.3.13 Ordinary laboratory equipment

Including a balance capable of weighing to an accuracy of 0,01 g and a desiccator with an efficient

desiccant (for example, silica gel).
6 Sampling of the preservative

Ensure that the sample of preservative shall be representative of the product to be tested. Samples shall

be stored and handled in accordance with any written recommendations from the supplier.

For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.

7 Test specimens
7.1 Wood species

The species of wood to be used shall be susceptible to attack by fungi and shall be readily impregnated

by liquids. The following species shall be used for the test:

Scots pine (Pinus sylvestris Linnaeus) sapwood for products intended to be used on softwoods.

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SIST EN 113-1:2021
EN 113-1:2020 (E)
Beech (Fagus sylvatica Linnaeus) for products intended to be used on hardwoods.

For testing products intended to be used on hardwoods against brown rot, Scots pine sapwood shall be

used. Scots pine sapwood is a representative matrix for efficacy testing with brown rot fungi.

Additional tests may be undertaken using other wood species corresponding to the above characteristics,

and of particular importance for certain countries, but if so, this shall be stated in the test report.

7.2 Wood quality

The wood shall be free from cracks, stain, decay, insect damage or other defects. The wood shall not have

been water-stored, floated, chemically treated or steamed or kiln-dried above 60 °C.

The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 annual growth

rings per 10 mm and 8 annual growth rings per 10 mm. The proportion of late wood in the annual rings

shall not exceed 30 % of the whole.

The beech shall be even-grained, free from tyloses, discolouration and red heart. It shall have between 2

and 6 annual growth rings per 10 mm.
7.3 Provision of test specimens

Condition the wood to a mass fraction of (12 ± 2) % moisture content or conditioned at (65 ± 5) % RH

(relative humidity) and (20 ± 2) °C for at least 2 weeks. Cut the test specimens from planed strips having

a cross section of (25 × 15) mm, on which the growth rings may run in any direction with the exception

of a completely tangential orientation on the broad faces which is unacceptable.

The longitudinal faces shall be parallel to the direction of the grain. Transverse cuts shall be made neatly

to give sharp edges.

The test specimens shall originate from a minimum of three trees or shall be taken at random from a

stock originally of more than 500 specimens and originating from at least 5 planks.

7.4 Dimensions and density of test specimens

The dimensions of the test specimens, measured at a mass fraction of (12 ± 2) % moisture content or

conditioned at (65 ± 5) % RH (relative humidity) and 20 ± 2 °C for at least 2 weeks, shall be (50 ± 0,5)

mm x (25 ± 0,5) mm x (15 ± 0,5) mm.

NOTE 1 A two-prong electrical conductivity moisture meter is suitable for assessing moisture content.

The dimensions of at least 10 specimens should be controlled randomly. If they are within the required

specifications, it is not necessary to measure the whole batch. If one of the controlled specimen does not

fulfil the requirement all specimens in the batch should be measured.

The volume of each test specimen is theoretically 18,75 cm , but the dimensions of each test specimen

shall be checked so that the actual volume is known. If the tolerance is no more than ± 0,2 mm, the volume

of all specimens can be taken as 18,75 cm .

In a batch of treated specimens, the density of an individual specimen is permitted to differ by ± 10 %

from the mean value of the batch. This tolerance is increased to ± 20 % for the untreated test specimens.

The mean density of the test specimens used for the test shall be recorded in the test report.

7.5 Number and distribution of test specimens
The specimens are divided into:
— e Treated specimens;
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SIST EN 113-1:2021
EN 113-1:2020 (E)

— e Test specimens: these are the impregnated specimens (submitted to drying, conditioning,

1.1

and any appropriate ageing) subjected to attack by the wood destroying fungi. Use at least four

treated test specimens for each preservative concentration (including a solvent or diluent

control (concentration = 0)), for each fungus and for each wood species.

— e Check test specimens for calculation of the correction value: These are test specimens

1.2

treated in exactly the same way as the e test specimens, at least four per concentration, which

1.1

are placed; after drying, conditioning and any appropriate ageing in uninoculated culture

vessels, two in each vessel. Variations in mass of these specimens make it possible to determine

the correction value (C) of the variations in mass of the treated test specimens (e1.1) resulting

from factors other than attack by the test fungi (see 8.6.2).
— e Untreated specimens;

— e Control specimens: These are non-impregnated test specimens, equal in number to the

2.1

treated test specimens e and of the same wood species which are placed one in each culture

1.1
vessel with the treated test specimens.
— e Virulence control specimens: Six of these non-impregnated specimens of the
2.2

appropriate wood species are subjected to attack by each wood destroying fungus.

Mark each specimen so that it can be identified throughout the test.

It is advisable to treat more specimens than the minimum number required to select those having the

uptake nearest to the target.
8 Procedure
8.1 Conditioning of test specimens before treatment

Place the numbered test specimens in the oven (see 5.3.3) and leave them there for 18 h to 24

...

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