SIST-TS CEN/TS 15360:2006

Fertilizers - Determination of dicyandiamide - Method using high-performance liquid chromatography (HPLC)

This Technical Specification specifies a method for the selective determination of dicyandiamide (DCD) in addition to all the other forms of nitrogen fixations, particularly in fertilizers to which DCD has been added as a nitrification inhibiting agent.

Düngemittel - Bestimmung von Dicyandiamid - Verfahren mit Hochleistungs­Flüssigchromatographie (HPLC)

Engrais - Détermination de la teneur en dicyandiamide - Méthode par chromatographie liquide a haute performance (HPLC)

Gnojila – Določevanje dicianodiamida – Metoda tekočinske kromatografije visoke ločljivosti (HPLC)

General Information

• Status: Withdrawn
• Publication Date: 31-Aug-2006
• Withdrawal Date: 09-Oct-2007
• ICS: 65.080 - Fertilizers
• Technical Committee: KAT - Soil quality
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 09-Oct-2007
• Due Date: 01-Nov-2007
• Completion Date: 10-Oct-2007
• Mandate: M/335 - Standardization Mandate assigned to the CEN concerning the modernisation of the methods of anylysis of Fertilizers
• Ref Project: CEN/TS 15360:2006 - Fertilizers - Determination of dicyandiamide - Method using high-performance liquid chromatography (HPLC)

Relations

• Replaced By: SIST EN 15360:2007 - Fertilizers - Determination of dicyandiamide - Method using high-performance liquid chromatography (HPLC)
• Effective Date: 01-Nov-2007

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 15360:2006
01-september-2006
*QRMLOD±'RORþHYDQMHGLFLDQRGLDPLGD±0HWRGDWHNRþLQVNHNURPDWRJUDILMHYLVRNH
ORþOMLYRVWL+3/&
Fertilizers - Determination of dicyandiamide - Method using high-performance liquid
chromatography (HPLC)
Düngemittel - Bestimmung von Dicyandiamid - Verfahren mit Hochleistungs­
Flüssigchromatographie (HPLC)
Engrais - Détermination de la teneur en dicyandiamide - Méthode par chromatographie
liquide a haute performance (HPLC)
Ta slovenski standard je istoveten z: CEN/TS 15360:2006
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 15360:2006 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

TECHNICAL SPECIFICATION
CEN/TS 15360
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
March 2006
ICS 65.080

English Version
Fertilizers - Determination of dicyandiamide - Method using high-
performance liquid chromatography (HPLC)
Engrais - Détermination de la teneur en dicyandiamide - Düngemittel - Bestimmung von Dicyandiamid - Verfahren
Méthode par chromatographie liquide à haute performance mit Hochleistungs-Flüssigchromatographie (HPLC)
(HPLC)
This Technical Specification (CEN/TS) was approved by CEN on 30 January 2006 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 15360:2006: E
worldwide for CEN national Members.

---------------------- Page: 2 ----------------------

CEN/TS 15360:2006 (E)
Contents Page
Foreword.3
1 Scope .4
2 Normative references .4
3 Principle.4
4 Reagents.4
5 Apparatus .4
6 Sampling and sample preparation.5
7 Procedure .5
8 Calculation.5
9 Precision.6
10 Notes on procedure.7
11 Test report .7
Annex A (informative) Results of the inter-laboratory trial .8
Bibliography .9

2

---------------------- Page: 3 ----------------------

CEN/TS 15360:2006 (E)
Foreword
This Technical Specification (CEN/TS 15360:2006) has been prepared by Technical Committee CEN/TC 260
“Fertilizers and liming materials”, the secretariat of which is held by DIN.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden,
Switzerland and the United Kingdom.
3

---------------------- Page: 4 ----------------------

CEN/TS 15360:2006 (E)
1 Scope
This Technical Specification specifies a method for the selective determination of dicyandiamide (DCD) in
addition to all the other forms of nitrogen fixations, particularly in fertilizers to which DCD has been added as a
nitrification inhibiting agent.
2 Normative references
The following referenced documents are indispensable for the application of this Technical Specification. For
dated references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
prEN 1482-2, Fertilizers and liming materials — Sampling and sample preparation — Part 2: Sample
preparation
EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696:1987)
3 Principle
The sample is dissolved or suspended in water, using an ultrasonic bath. To the filtered solution, methyl
dicyandiamide is added as the internal standard, the solution is transferred onto a C18 reversed-phase
column using a bypass injector and then separated. For the detection an UV-detector is used at a wavelength
of 220 nm.
4 Reagents
Use only reagents of recognized analytical grade, and water conforming to grade 2 of EN ISO 3696.
4.1 Dicyandiamide standard solution
Weigh 50 mg of dicyandiamide of known purity into a 1 000 ml volumetric flask and dissolve in water, and
make up the volume to the mark. Pipette 10 ml of this solution into a 100 ml volumetric flask and, after having
added 10 ml of internal standard solution (4.2), make up the volume to the mark with water.
4.2 Internal standard solution
Weigh 50 mg of methyl dicyandiamide of known purity into a 1 000 ml volumetric flask, dissol
...