SIST EN 16772:2016

Water quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers

This European Standard provides guidance on methods for sampling invertebrates in the hyporheic zone of wadable rivers. It describes each method, including details of the equipment involved and its use in the field. Guidance is given on developing a sampling strategy and selecting an appropriate survey technique for the purpose of investigation.
NOTE   Benthic macroinvertebrate sampling is covered by other published standards (see Bibliography). Selected literature with references in support of this document is given in the Bibliography.

Wasserbeschaffenheit - Anleitung für die Probenahme von Invertebraten (Wirbellosen) in der hyporheischen Zone von Flüssen

Qualité de l'eau - Lignes directrices relatives aux méthodes d'échantillonnage des invertébrés dans la zone hyporhéique de rivières

La présente Norme européenne fournit des lignes directrices relatives à l’échantillonnage des invertébrés dans la zone hyporhéique de rivières prospectables à pied. Elle décrit chaque méthode, y compris les détails du matériel nécessaire et son utilisation sur le terrain. Des lignes directrices sont données sur l’élaboration d’une stratégie d’échantillonnage et la sélection d’une technique de sondage appropriée pour l’étude.
NOTE L’échantillonnage de macro-invertébrés benthiques est traité dans d’autres normes publiées (voir la Bibliographie). La littérature sélectionnée dont les références étayent le présent document est indiquée dans la Bibliographie.

Kakovost vode - Navodilo za metode vzorčenja nevretenčarjev v hiporeični coni rek

Ta dokument podaja navodila za metode vzorčenja nevretenčarjev v hiporhaičnih conah prebrodljivih rek. Opisuje vsako metodo, vključno s podrobnostmi o vključeni opremi in njeni uporabi na terenu. Podana so navodila o razvijanju strategije vzorčenja in izbiranju ustrezne tehnike raziskovanja za namen preiskave. Vzorčenje bentoških makronevretenčarjev je obravnavano v drugih objavljenih standardih (glej bibliografijo). Izbrana literatura s sklici v podporo temu dokumentu je podana v bibliografiji.

General Information

• Status: Published
• Public Enquiry End Date: 05-Nov-2014
• Publication Date: 09-Nov-2016
• ICS: 13.060.10 - Water of natural resources
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: KAV - Water quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 07-Nov-2016
• Due Date: 12-Jan-2017
• Completion Date: 10-Nov-2016
• Directive: 2000/60/EC - Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy (substitutes Directive Reference 01/991 - Common Position on WFD)
• Ref Project: EN 16772:2016 - Water quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers

Overview

EN 16772:2016 - Water quality: Guidance on methods for sampling invertebrates in the hyporheic zone of rivers (CEN) - provides practical, field-focused guidance for collecting and assessing invertebrate fauna living within the saturated sediments beneath and alongside wadable river channels (the hyporheic zone). The standard describes sampling equipment and field procedures, advises on developing a sampling strategy, and helps users select appropriate survey techniques for specific study objectives. It complements, but does not replace, standards for benthic macroinvertebrate sampling.

Key topics and requirements

• Scope and definitions: Clear definitions for hyporheic terms (hyporheic zone, hyporheos, benthos, meiofauna, macroinvertebrate) and the standard’s focus on wadable rivers.
• Survey objectives: Emphasises defining aims before method selection; suitability of methods depends on whether the study targets diversity, abundance, biomass, or impact assessments.
• Sampling strategy: Guidance on designing spatial and temporal sampling (reach selection, substrate types, instream vs riparian locations) and on quantitative vs semi‑quantitative approaches.
• Detailed sampling methods: Descriptions and field use of techniques and equipment, including (as covered in the standard):
  • Karaman‑Chappuis pit
  • Bou‑Rouch pump
  • Vacuum pump
  • Standpipe trap
  • Williams corer
  • Colonization devices
  • Freeze coring Each method includes notes on species typically sampled and relevant environmental variables.
• Sample processing: Field and laboratory handling recommendations to ensure data quality.
• Safety and adaptability: Emphasises health & safety compliance and that methods may need modification for local conditions.

Practical applications

• River monitoring and ecological status assessments under water‑quality programmes
• Catchment management, river restoration and impact studies (pollution, habitat alteration, fish spawning nursery evaluation)
• Scientific research into hyporheic biodiversity, biogeochemical processes and surface–groundwater interactions
• Consultants and environmental contractors designing targeted invertebrate sampling campaigns

Who should use it

• Environmental agencies and regulators
• Hydrogeologists and stream ecologists
• Water quality scientists and biodiversity researchers
• Environmental consultants and field survey teams

Related standards and policy links

• Complements standards for benthic macroinvertebrate sampling (see Bibliography)
• Relevant EU legislation referenced in the standard: Habitats Directive, Water Framework Directive, Groundwater Directive, Nitrates Directive
• For detailed taxonomic or laboratory protocols consult the standard’s Bibliography and national guidance

Keywords: EN 16772, hyporheic zone, hyporheos, sampling invertebrates, water quality, CEN standard, river monitoring, sampling methods.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.UHNWasserbeschaffenheit - Anleitung für die Probenahme von Invertebraten (Wirbellosen) in der hyporheischen Zone von FlüssenQualité de l'eau - Lignes directrices relatives aux méthodes d'échantillonnage des invertébrés dans la zone hyporhéique de rivièresWater quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 16772:2016SIST EN 16772:2016en,fr,de01-december-2016SIST EN 16772:2016SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16772
April
t r s x ICS
s uä r x rä y r English Version
Water quality æ Guidance on methods for sampling invertebrates in the hyporheic zone of rivers Qualité de l 5eau æ Lignes directrices relatives aux méthodes d 5échantillonnage des invertébrés dans la zone hyporhéique des rivières
Wasserbeschaffenheit æ Anleitung zu Methoden für die hyporheischen Zone von Flüssen This European Standard was approved by CEN on
t r February
t r s xä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
t r s x CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x y y tã t r s x ESIST EN 16772:2016

Examples of sampling strategies for three different types of investigation . 21 A.1 Case Study 1 – Assessing regional biodiversity and species richness . 21 A.2 Case Study 2 – Assessing impacts on fish spawning sites . 21 A.3 Case Study 3 – Assessing the impacts of pollution . 21 Bibliography . 22
Karaman-Chappuis pit Bou-Rouch pump Vacuum pump Standpipe trap Williams corer Colonization devices Freeze coring Migration/dispersal No No No Yes No Yes No Spatial heterogeneity No Yes Yes Yes Yes Yes Yes Temporal variability Yes Yes Yes Yes Yes Yes Yes Interstitial sediment transport No No No Yes No Yes No Substrate characteristics No No No No No No Yes Used on submerged substrate No Yes Yes Yes Yes Yes Yes Used in riparian zone Yes Yes Yes Yes Yes Yes Yes Quantitative No No No No Yes Yes Yes Semi-quantitative No Yes Yes Yes Yes No No 4 Sampling strategy The design of a sampling strategy will vary according to the purpose of the investigation. Sampling site location may be influenced by pre-existing monitoring networks or previous investigations. The following should be considered: — sampling method; — number and location of sampling sites; — number of replicates per site required to characterize site heterogeneity (e.g. upwelling and downwelling, substrate characteristics); — sampling frequency; — sampling depth; — seasonal variability; — abiotic data requirements; — spatial and temporal scale of investigation. Scale is important when examining the hyporheic zone, as various processes occur at different spatial scales. For example, at a stream-bed (patch) scale the size, shape, sorting and stability of unconsolidated material are the primary determinants of porosity and permeability. These factors have a major influence on community composition over relatively short distances and methods have been developed to address this [11]. At a broader scale, lateral connectivity (e.g. between the riparian zone and out to the wider floodplain) is a key consideration. Hyporheic flow paths occur at multiple scales, from the stream bed to the catchment. SIST EN 16772:2016

Karaman-Chappuis pit Bou-Rouch pump Vacuum pump Stand-pipe trap Williams corer Coloniz-ation devices Freeze coring Minimum number of operators a 1 1 1 1 1 1 2 Equipment cost
(in Euro, 2016) < 200 < 1 000 < 350 < 200 < 1 000 < 500 < 10 000 Installation time ~15 min ~15 min ~15 min ~2 h ~15 min ~1 h ~30 min Time to collect one sample ~5 min ~10 min ~10 min ~10 min ~5 min ~10 min ~45 min Portability High Medium High Medium High Medium Low Typical recovery time after installation None None None 2 weeks None Variable Variable Repeat sampling possible No No Yes Yes No Yes No a Installation of equipment for some methods will require additional help, i.e. for the standpipe traps, colonization devices and freeze coring. 5.2 Karaman-Chappuis pit 5.2.1 Description and operation This is a rapid, qualitative method for obtaining a hyporheic sample from exposed river sediments, particularly in gravel and sand [14]. A pit (~50 cm diameter) is excavated to such a depth that its base is below the water level. Interstitial flow into the pit is maintained by extracting water, using a hand pump SIST EN 16772:2016

Key 1 safety pin 9 bolt 2 lever 10 screen 3 upper piston valve 11 tip 4 liner 12 hand piston pump 5 body of the pump 13 mobile standpipe 6 lower piston 14 hyporheic zone 7 lower valve 15 standpipe with screen with 5-mm diameter ho
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