SIST EN ISO 24211:2022

Vapour products - Determination of selected carbonyls in vapour product emissions (ISO 24211:2022)

This document specifies a method for the determination of the amount of selected carbonyl compounds (formaldehyde and acetaldehyde) as their 2,4-dinitrophenylhydrazones in vapour product emissions using reversed phase liquid chromatography coupled with ultraviolet or diode array detector (LC-UV or LC-DAD).
This document does not include the analysis of other carbonyl compounds, such as acrolein and crotonaldehyde, due to previous work indicated issues associated with stability of these compounds in the e-liquid solutions that were used to evaluate method performance[4].

Dampfprodukte - Bestimmung von ausgewählten Carbonylen in Emissionen von Dampfprodukten (ISO 24211:2022)

Dieses Dokument legt ein Verfahren für die Bestimmung der Menge ausgewählter Carbonylverbindungen (Formaldehyd und Acetaldehyd) als ihre 2,4-Dinitrophenylhydrazine in den Emissionen von Dampfprodukten mittels Umkehrphasen-Flüssigkeitschromatographie in Verbindung mit einem Ultraviolett- oder Diodenarray-Detektor (LC-UV oder LC-DAD) fest.
Die Analyse anderer Carbonylverbindungen wie Acrolein und Crotonaldehyd ist nicht Teil dieses Dokuments, da frühere Arbeiten auf Probleme im Zusammenhang mit der Stabilität dieser Verbindungen in den E Liquid-Lösungen hinwiesen, die zur Bewertung der Leistungsfähigkeit des Verfahrens verwendet wurden [4].
WARNUNG — Mit der Anwendung dieses Dokuments kann der Einsatz gefährlicher Stoffe, Arbeitsgänge und Geräte einhergehen. Dieses Dokument erhebt nicht den Anspruch, alle mit seiner Anwendung einhergehenden Sicherheitsprobleme zu behandeln. Es liegt in der Verantwortung des Anwenders dieses Dokumentes, vor der Anwendung angemessene Maßnahmen in Bezug auf den Gesundheits- und Arbeitsschutz zu ergreifen und die Anwendbarkeit anderer Beschränkungen zu bestimmen.

Produits de vapotage - Dosage de carbonyles sélectionnés dans les émissions de produits de vapotage (ISO 24211:2022)

Le présent document spécifie une méthode de dosage de composés carbonylés sélectionnés (formaldéhyde et acétaldéhyde) sous la forme de leurs 2,4-dinitrophénylhydrazones dans les émissions de produits de vapotage par chromatographie liquide en phase inverse couplée à un détecteur UV ou à barrette de diodes (CL-UV ou CL-DBD).
Le présent document n'inclut pas l'analyse d'autres composés carbonylés, tels que l'acroléine et le crotonaldéhyde, en raison de problèmes d’instabilité de ces composés dans les solutions d’e-liquides qui ont été utilisées pour évaluer la performance de la méthode[4], problèmes signalés lors de travaux antérieurs.

Hlapni proizvodi - Ugotavljanje deleža izbranih karbonilov v emisijah hlapnih proizvodov (ISO 24211:2022)

Ta dokument določa metodo za ugotavljanje deleža izbranih karbonilnih spojin v emisijah e-hlapnih proizvodov.
Izbrane so naslednje karbonilne spojine:
• acetaldehid
• formaldehid
• akrolein (potencialno)
• krotonaldehid (potencialno)

General Information

• Status: Published
• Public Enquiry End Date: 30-Nov-2021
• Publication Date: 09-Oct-2022
• ICS: 65.160 - Tobacco, tobacco products and related equipment
• Technical Committee: I13 - Imaginarni 13
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 28-Sep-2022
• Due Date: 03-Dec-2022
• Completion Date: 10-Oct-2022
• Ref Project: EN ISO 24211:2022 - Vapour products - Determination of selected carbonyls in vapour product emissions (ISO 24211:2022)

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2022
Hlapni proizvodi - Ugotavljanje deleža izbranih karbonilov v emisijah hlapnih
proizvodov (ISO 24211:2022)
Vapour products - Determination of selected carbonyls in vapour product emissions (ISO
24211:2022)
Dampfprodukte - Bestimmung von ausgewählten Carbonylen in Emissionen von
Dampfprodukten (ISO 24211:2022)
Produits de vapotage - Dosage de carbonyles sélectionnés dans les émissions de
produits de vapotage (ISO 24211:2022)
Ta slovenski standard je istoveten z: EN ISO 24211:2022
ICS:
65.160 Tobak, tobačni izdelki in Tobacco, tobacco products
oprema and related equipment
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 24211
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2022
EUROPÄISCHE NORM
ICS 65.160
English Version
Vapour products - Determination of selected carbonyls in
vapour product emissions (ISO 24211:2022)
Produits de vapotage - Dosage de carbonyles Dampfprodukte - Bestimmung von ausgewählten
sélectionnés dans les émissions de produits de Carbonylen in Emissionen von Dampfprodukten (ISO
vapotage (ISO 24211:2022) 24211:2022)
This European Standard was approved by CEN on 30 August 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 24211:2022 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 24211:2022) has been prepared by Technical Committee ISO/TC 126 "Tobacco
and tobacco products" in collaboration with Technical Committee CEN/TC 437 “Electronic cigarettes
and e-liquids” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2023, and conflicting national standards shall
be withdrawn at the latest by March 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 24211:2022 has been approved by CEN as EN ISO 24211:2022 without any modification.

INTERNATIONAL ISO
STANDARD 24211
First edition
2022-08
Vapour products — Determination of
selected carbonyls in vapour product
emissions
Produits de vapotage — Dosage de carbonyles sélectionnés dans les
émissions de produits de vapotage
Reference number
ISO 24211:2022(E)
ISO 24211:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 24211:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
5.1 General . 2
5.2 Solution preparation . 3
5.3 Preparation of standards . 3
5.3.1 General . 3
5.3.2 HPLC calibration standards and working solutions . 3
5.3.3 Primary carbonyl standards . 3
5.3.4 Secondary carbonyl standards . 4
5.3.5 Carbonyl working standards . 4
6 Apparatus . 4
7 Procedure .5
7.1 Preparation of test samples . 5
7.2 Glass fibre filter pads handling . 5
7.3 Aerosol collection and sample preparation . 5
7.4 Determination of aerosol collected mass (ACM) and e-liquid vaporised mass (EVM) . 6
7.5 Test portion . 6
7.6 Setting up the apparatus . 7
7.7 Calibration of the HPLC system . 8
7.8 Determination . 8
8 Expression of the results .8
9 Repeatability and reproducibility .9
9.1 General . 9
9.2 Results of an interlaboratory study . 9
10 Test report .11
Annex A (informative) DNPH solution (prepared with DNPH containing approximately
30 % water) .12
Annex B (informative) Example of calibration standards preparation .14
Annex C (informative) HPLC chromatogram of a typical aerosol sample containing
carbonyls .15
Bibliography .16
iii
ISO 24211:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products,
Subcommittee SC 3, Vape and vapour products, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 437, Electronic cigarettes and e-liquids, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
ISO 24211:2022(E)
Introduction
In many countries, regulation of vapour products requires reporting for carbonyl compounds in
emissions. Therefore, there is a necessity to have an International Standard in place to get reliable/
comparable data for selected carbonyls in vapour product emissions.
[1]
The method in this document is based upon the CORESTA recommended method CRM 96 which was
written on the basis of the results obtained in an interlaboratory study conducted in 2019 involving 11
laboratories.
Carbonyl compounds are known to be derived from the thermal degradation of the base ingredients
[2],[3]
of the e-liquid formulations. The experimental design parameters used to collect the aerosolised
vapour should be evaluated and documented for each analysis.
v
INTERNATIONAL STANDARD ISO 24211:2022(E)
Vapour products — Determination of selected carbonyls in
vapour product emissions
WARNING — The use of this document can involve hazardous materials, operations and
equipment. This document does not purport to address all the safety problems associated with
its use. It is the responsibility of the user of this document to establish appropriate safety and
health practices, and determine the applicability of any other restrictions prior to use.
1 Scope
This document specifies a method for the determination of the amount of selected carbonyl compounds
(formaldehyde and acetaldehyde) as their 2,4-dinitrophenylhydrazones in vapour product emissions
using reversed phase liquid chromatography coupled with ultraviolet or diode array detector (LC-UV
or LC-DAD).
This document does not include the analysis of other carbonyl compounds, such as acrolein and
crotonaldehyde, due to previous work indicated issues associated with stability of these compounds in
[4]
the e-liquid solutions that were used to evaluate method performance .
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 20768, Vapour products — Routine analytical vaping machine — Definitions and standard conditions
1)
ISO 24197:— , Vapour products — Determination of e-liquid vaporised mass and aerosol collected mass
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
aerosol collected mass
ACM
mass of aerosol collected on a glass fibre filter pad resulting from the operation of a vapour product by
a routine analytical vaping machine after a defined number of puffs
Note 1 to entry: Routine analytical vaping machine is covered by ISO 20768.
1) Under preparation. Stage at the date of publication: ISO/FDIS 24197:2022.
ISO 24211:2022(E)
3.2
e-liquid vaporised mass
EVM
mass of e-liquid transferred from the vapour product to the aerosol
Note 1 to entry: The term “vapour product mass loss” or “mass loss” refers to the e-liquid vaporised mass.
[SOURCE: ISO 24197:—, 3.3]
3.3
puff block
finite series of sequential puffs as defined by the user or by the test request
EXAMPLE Puff block: 1: puffs 1 to 50, puff block 2: puffs 51 to 100, puff block 3: puffs 101 to 150.
3.4
aerosol trapping system
system for collecting the aerosol from vapour products
Note 1 to entry: For this method the aerosol trapping system consists of a filter trap (pad + holder) and an
impinger in series.
3.5
reagent blank
solution that is evaluated to check the level of contamination introduced by the reagents
3.6
aerosol blank
sample from a port that is attached to an aerosol trapping system (3.4) that contains no vapour product
and is carried through the same collection, preparation and analysis steps as the test samples
4 Principle
The vapour product emissions are generated and collected on a vaping machine according to
ISO 20768. The trapping system that is used to trap carbonyls consists of a pad holder containing a
glass fibre filter pad according to ISO 24197, in series with an impinger containing an acidified
solution of 2,4-dinitrophenylhydrazine (DNPH) in 1:1 acetonitrile: water. Post-vaping, the glass fibre
filter pad is combined with the impinger solution and shaken mechanically for 20 min. An aliquot of
the sample extract is subsequently neutralised with pyridine and analysed by reversed phase liquid
chromatography with ultra violet or diode array detector (RPLC-UV or RPLC-DAD). The carbonyl
content in the vapour product emissions is calculated based on an external calibration curve containing
the prederivatized DNPH carbonyl compounds. Results are expressed as the weight of carbonyl per
puff, per aerosol collected mass (ACM) or per puff block as warranted.
5 Reagents
5.1 General
Use only reagents of recognized analytical grade.
NOTE Alternative reagents can be used provided the suitability and equivalence is verified.
5.1.1 Acetonitrile, ACN, HPLC grade.
5.1.2 Ethanol, HPLC grade.
5.1.3 Phosphoric acid, (H PO , a mass fraction of 85 %, or a volume fraction of 10 % aqueous
3 4
solution).
ISO 24211:2022(E)
5.1.4 Water, grade 1 according to ISO 3696 or equivalent.
5.1.5 Pyridine, minimum purity 99 %.
5.1.6 Formaldehyde-DNPH, minimum purity 99 %.
5.1.7 Acetaldehyde-DNPH, minimum purity 99 %.
5.1.8 2,4-Dinitrophenylhydrazine hydrochloride (DNPH-HCL) or 2,4-dinitrophenylhydrazine
(DNPH)(containing approximately 30 % water).
5.2 Solution preparation
5.2.1 General
Prepare appropriately proportioned amounts of the solutions listed below. All solutions shall be
equilibrated to room temperature prior to use. Use graduated cylinders and calibrated pipettes to
combine components.
5.2.2 10 % H PO
3 4
Prepare by bringing 118 ml of 85 % H PO (5.1.3) to 1 l water (5.1.4). Vendor prepared 10 % H PO may
3 4 3 4
be used instead. Store at room temperature.
5.2.3 DNPH trapping solution, prepared with DNPH-HCL (5.1.8)
Dissolve 1,0 g DNPH-HCl (5.1.8) in 500 ml of ACN (5.1.1), combine with 40 ml of 10 % H PO (5.2.2) and
3 4
bring to 1 l with water (5.1.4). Mix solution to ensure all the DNPH-HCl dissolves and no crystals remain.
Solution should be prepared fresh weekly, stored at room temperature and protected from light.
NOTE Alternative preparation of trapping solution with DNPH containing 30 % water is provided in Annex A.
5.2.4 Neutralised DNPH trapping solution (if dilutions are required)
Transfer 50 ml of DNPH trapping solution to a suitable size glass bottle and add 2,5 ml of pyridine. Mix
solution thoroughly.
5.3 Preparation of standards
5.3.1 General
All solutions shall be equilibrated to room temperature prior to use.
5.3.2 HPLC calibration standards and working solutions
The calibration should cover the concentration range of interest. Annex B provides a suitable
concentration range that can be used for the analysis, however, it can be adjusted depending on the
level of carbonyls detected in the samples. The user shall ensure the low calibration standard has a
sufficient signal to noise ratio for accurate quantitation (≥10:1) and that the calibration curve is linear.
5.3.3 Primary carbonyl standards
Weigh the hydrazones as described in Annex B into individual 25 ml volumetric flasks and dissolve in
acetonitrile. Record the concentrations of the free aldehyde equivalents in µg/ml.
...