SIST EN ISO 3093:2010
(Main)Wheat, rye and their flours, durum wheat and durum wheat semolina - Determination of the falling number according to Hagberg-Perten (ISO 3093:2009)
Wheat, rye and their flours, durum wheat and durum wheat semolina - Determination of the falling number according to Hagberg-Perten (ISO 3093:2009)
This International Standard specifies the determination of the a-amylase activity of cereals by the falling number (FN) method according to Hagberg-Perten. This method is applicable to cereal grains, in particular to wheat and rye and their flours, durum wheat and its semolina. This method is not applicable to the determination of low levels of a-amylase activity. By converting the FN into a liquefaction number (LN), it is possible to use this method to estimate the composition of mixtures of grain, flour or semolina with known FNs necessary to produce a sample of a required FN.
Weizen, Roggen und deren Mehl, Hartweizen und Hartweizengrieß - Bestimmung der Fallzahl nach Hagberg-Perten (ISO 3093:2009)
Diese Internationale Norm legt die Bestimmung der a-Amylaseaktivität von Getreide mit dem Fallzahl-(FN)-Verfahren nach Hagberg-Perten fest.
Dieses Verfahren ist auf Getreidesorten anwendbar, insbesondere auf Weizen und Roggen und deren Mehl, Hartweizen und Hartweizengrieß.
Das Verfahren ist nicht für die Bestimmung von geringen a-Amylaseaktivitäten anwendbar.
Durch Umwandlung der Fallzahl (FN) in eine Verflüssigungszahl (LN) ist es möglich, dieses Verfahren zum Abschätzen der Zusammensetzung von Gemischen aus Getreide, Mehl oder Grieß mit bekannten Fallzahl-werten anzuwenden, das zum Herstellen einer Probe mit der geforderten Fallzahl notwendig ist.
Blés tendres, seigles et leurs farines, blés durs et leurs semoules - Détermination de l'indice de chute selon Hagberg-Perten (ISO 3093:2009)
L'ISO 3093:2009 spécifie la détermination du niveau d'activité alpha-amylasique des céréales par la méthode dite de l'indice de chute (IC) selon Hagberg-Perten.
Cette méthode est applicable aux céréales en grains, en particulier au blé tendre et au seigle, et leurs farines, ainsi qu'au blé dur et ses semoules.
Cette méthode ne s'applique pas à la détermination des faibles niveaux d'activité alpha-amylasique.
Par conversion de l'IC en nombre de liquéfaction (NL), la méthode permet également d'estimer la composition de mélanges de grains, farines ou semoules d'IC connus nécessaire pour obtenir un produit d'IC déterminé.
Pšenica, rž in njune moke, pšenica durum in pšenični zdrob durum - Ugotavljanje števila padanja po Hagberg-Pertenu (ISO 3093:2009)
Ta mednarodni standard določa ugotavljanje aktivnosti a-amilaze v žitu z metodo števila padanja (FN) po Hagberg-Pertenu. Ta metoda velja za žitna zrna, zlasti za pšenico, rž in njune moke, pšenico durum in njen zdrob. Ta metoda ne velja za ugotavljanje nizkih ravni aktivnosti a-amilaze. S pretvorbo FN v število utekočinjanja (LN) se ta metoda lahko uporabi za oceno sestave mešanic zrn, moke ali zdroba z znanimi števili padanja, ki je potrebna za pripravo vzorca z zahtevanim številom padanja.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 3093:2010
01-marec-2010
1DGRPHãþD
SIST EN ISO 3093:2007
SIST EN ISO 3093:2007/AC:2009
3ãHQLFDUåLQQMXQHPRNHSãHQLFDGXUXPLQSãHQLþQL]GUREGXUXP8JRWDYOMDQMH
ãWHYLODSDGDQMDSR+DJEHUJ3HUWHQX ,62
Wheat, rye and their flours, durum wheat and durum wheat semolina - Determination of
the falling number according to Hagberg-Perten (ISO 3093:2009)
Weizen, Roggen und deren Mehl, Hartweizen und Hartweizengrieß - Bestimmung der
Fallzahl nach Hagberg-Perten (ISO 3093:2009)
Blés tendres, seigles et leurs farines, blés durs et leurs semoules - Détermination de
l'indice de chute selon Hagberg-Perten (ISO 3093:2009)
Ta slovenski standard je istoveten z: EN ISO 3093:2009
ICS:
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
SIST EN ISO 3093:2010 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 3093:2010
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SIST EN ISO 3093:2010
EUROPEAN STANDARD
EN ISO 3093
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2009
ICS 67.060 Supersedes EN ISO 3093:2007
English Version
Wheat, rye and their flours, durum wheat and durum wheat
semolina - Determination of the falling number according to
Hagberg-Perten (ISO 3093:2009)
Blés tendres, seigles et leurs farines, blés durs et leurs Weizen, Roggen und deren Mehl, Hartweizen und
semoules - Détermination de l'indice de chute selon Hartweizengrieß - Bestimmung der Fallzahl nach Hagberg-
Hagberg-Perten (ISO 3093:2009) Perten (ISO 3093:2009)
This European Standard was approved by CEN on 1 December 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 3093:2009: E
worldwide for CEN national Members.
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SIST EN ISO 3093:2010
EN ISO 3093:2009 (E)
Contents Page
Foreword .3
2
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SIST EN ISO 3093:2010
EN ISO 3093:2009 (E)
Foreword
This document (EN ISO 3093:2009) has been prepared by Technical Committee ISO/TC 34 "Food products"
in collaboration with Technical Committee CEN/TC 338 “Cereal and cereal products” the secretariat of which
is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2010, and conflicting national standards shall be withdrawn at
the latest by June 2010.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 3093:2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 3093:2009 has been approved by CEN as a EN ISO 3093:2009 without any modification.
3
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SIST EN ISO 3093:2010
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SIST EN ISO 3093:2010
INTERNATIONAL ISO
STANDARD 3093
Fourth edition
2009-12-15
Wheat, rye and their flours, durum wheat
and durum wheat semolina —
Determination of the falling number
according to Hagberg-Perten
Blés tendres, seigles et leurs farines, blés durs et leurs semoules —
Détermination de l'indice de chute selon Hagberg-Perten
Reference number
ISO 3093:2009(E)
©
ISO 2009
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SIST EN ISO 3093:2010
ISO 3093:2009(E)
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ii © ISO 2009 – All rights reserved
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SIST EN ISO 3093:2010
ISO 3093:2009(E)
Contents Page
Foreword .iv
1 Scope.1
2 Normative references.1
3 Terms and definitions .1
4 Principle.2
5 Reagents.2
6 Apparatus.2
7 Sampling.3
8 Preparation of test sample .3
8.1 Whole grain .3
8.2 Flour and semolina samples .4
9 Procedure.4
9.1 Determination of the moisture content .4
9.2 Test portion.4
9.3 Determination of falling number .5
9.4 Calculation .6
10 Precision.7
10.1 Interlaboratory tests.7
10.2 Repeatability .7
10.3 Reproducibility .7
11 Test report.8
Annex A (normative) Equations for altitude correction of falling numbers .9
Annex B (informative) Results of interlaboratory tests .10
Bibliography.13
© ISO 2009 – All rights reserved iii
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SIST EN ISO 3093:2010
ISO 3093:2009(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
ISO draws attention to the fact that it is claimed that compliance with this document may involve the use of a
patent concerning the falling number apparatus specified in 6.1.
ISO takes no position concerning the evidence, validity and scope of this patent right.
The holder of this patent right has assured the ISO that he is willing to negotiate licences under reasonable
and non-discriminatory terms and conditions with applicants throughout the world. In this respect, the
statement of the holder of this patent right is registered with the ISO. Information may be obtained from:
Perten Instruments AB
P.O. Box 5101
S-141 05 HUDDINGE
Sweden
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights other than those identified above. ISO shall not be held responsible for identifying any or all such patent
rights.
ISO 3093 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4, Cereals
and pulses.
This fourth edition cancels and replaces the third edition (ISO 3093:2004), of which it constitutes a minor
revision. It also incorporates the Technical Corrigendum, ISO 3093:2004/Cor.1:2008.
iv © ISO 2009 – All rights reserved
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SIST EN ISO 3093:2010
INTERNATIONAL STANDARD ISO 3093:2009(E)
Wheat, rye and their flours, durum wheat and durum wheat
semolina — Determination of the falling number according to
Hagberg-Perten
1 Scope
This International Standard specifies the determination of the a-amylase activity of cereals by the falling
number (FN) method according to Hagberg-Perten.
This method is applicable to cereal grains, in particular to wheat and rye and their flours, durum wheat and its
semolina.
This method is not applicable to the determination of low levels of a-amylase activity.
By converting the FN into a liquefaction number (LN), it is possible to use this method to estimate the
composition of mixtures of grain, flour or semolina with known FNs necessary to produce a sample of a
required FN.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 712, Cereals and cereal products — Determination of moisture content — Reference method
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
falling number
FN
t
total time required to activate a viscometer stirrer and allow it to fall a predetermined distance through an
aqueous gel prepared from heating a mixture of flour or semolina, and water in a viscometer tube, and which
is undergoing liquefaction due to attack by the enzyme a-amylase
NOTE 1 Time is counted from immersion in the water bath.
NOTE 2 The falling number is expressed in seconds.
© ISO 2009 – All rights reserved 1
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SIST EN ISO 3093:2010
ISO 3093:2009(E)
3.2
liquefaction number
LN
n
L
result of a simple calculation to convert the falling number (3.1) into a value used to estimate the composition
of mixtures of grain, flour or semolina necessary to produce a sample of the required falling number
NOTE LNs, unlike FNs, are additive.
4 Principle
The a-amylase activity is estimated using the starch present in the sample as a substrate. The determination
is based on the ability of an aqueous suspension of flour, semolina or wholemeal cereal product to gelatinize
rapidly in a boiling water bath, and on the measurement of starch liquefaction by the a-amylase present in the
sample.
Liquefaction affects the thickness of the starch gel and, hence, the resistance to the viscometer stirrer and the
time taken for it to fall a defined distance.
5 Reagents
5.1 Water, produced by distillation or demineralization, complying with ISO 3696, grade 3.
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
1)
6.1 Apparatus for FN determination , comprising the following components.
6.1.1 Water bath, with integral heating unit, cooling system and water level indicator.
6.1.2 Electronic timer.
6.1.3 Viscometer stirrer, metallic, able to move freely within the ebonite stopper.
Its rods shall be straight and the wheels free from distortion and wear.
6.1.4 Precision viscometer tubes, manufactured from special glass, with the following dimensions:
⎯ inner diameter: 21,00 mm ± 0,02 mm;
⎯ outer diameter: 23,80 mm ± 0,25 mm;
⎯ inner height: 220,0 mm ± 0,3 mm.
6.1.5 Rubber stoppers, to fit the viscometer tubes.
[4]
6.2 Automatic dispenser or pipette, ISO 8655-2 , allowing a volume of 25,0 ml ± 0,2 ml to be delivered.
1) The “Falling Number” apparatus having a specifically designed viscometer stirrer produced by Perten Instruments is
an example of a suitable device available commercially. This device forms the subject of a patent. This information is
given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this
product.
2 © ISO 2009 – All rights reserved
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SIST EN ISO 3093:2010
ISO 3093:2009(E)
6.3 Analytical balance, capable of being read to the nearest 0,01 g.
2)
6.4 Laboratory mill , hammer type, and fitted with a 0,8 mm screen allowing the production of a
wholemeal product meeting the particle size specification shown in 8.1.3.
Check the performance of the mill periodically using a well-mixed sample of ground grain (as produced
in 8.1.2).
The mill may be equipped with an automatic feeding device, particularly for the grinding of high moisture
content grain.
[1] [2]
6.5 Laboratory sieve, of nominal size of openings 800 µm, ISO 565 and ISO 3310 (all parts) .
7 Sampling
Sampling is not part of the method specified in this International Standard. A recommended sampling method
[5]
is given in ISO 13690 .
A representative sample should have been sent to the laboratory. It should not have
...
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