SIST EN ISO 11816-1:2014
(Main)Milk and milk products - Determination of alkaline phosphatase activity - Part 1: Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2013)
Milk and milk products - Determination of alkaline phosphatase activity - Part 1: Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2013)
ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816-1:2013)
Dieser Teil von ISO 11816 | IDF 155 legt ein fluorimetrisches Verfahren für die Bestimmung der Aktivität der alkalischen Phosphatase (ALP, EC 3.1.3.1) in Rohmilch und pasteurisierter Vollmilch, fettarmer Milch, Magermilch und aromatisierter Milch fest. Dieses Verfahren ist für Milch und flüssige Milchprodukte von Kühen, Schafen und Ziegen anwendbar. Es ist auch auf Milchpulver nach Rekonstituierung anwendbar.
Das Gerät kann Aktivitäten von bis zu 7 000 Milliunits je Liter (mU/l) auslesen. Wenn die Aktivität größer als 7 000 mU/l ist, dann ist mit Milch, die frei von alkalischer Phosphatase (7.1) ist, zu verdünnen, um ein Ergebnis kleiner als 7 000 mU/l zu erhalten.
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1: Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2013)
L'ISO 11816-1|FIL 155-1:2013 spécifie une méthode fluorimétrique pour la détermination de l'activité de la phosphatase alcaline (ALP, EC 3.1.3.1) dans les laits crus et traités thermiquement entiers, demi-écrémés, écrémés et dans les laits aromatisés. La méthode est applicable au lait et aux boissons à base de lait de vache, de brebis et de chèvre. Elle est également applicable au lait en poudre après reconstitution.
L'appareillage peut lire des activités jusqu'à 7 000 milliunités par litre (mU/l). Si l'activité est supérieure à 7 000 mU/l, une dilution avec du lait exempt de phosphatase alcaline (7.1) est effectuée de façon à obtenir un mesurage ne dépassant pas 7 000 mU/l.
Mleko in mlečni proizvodi - Določevanje aktivnosti alkalne fosfataze - 1. del: Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2013)
Ta del standarda ISO 11816|IDF 155 določa fluorimetrijsko metodo za določevanje aktivnosti alkalne fosfataze (ALP, EC 3.1.3.1) v surovem in toplotno obdelanem polnomastnem mleku, delno posnetem mleku, posnetem mleku in aromatiziranem mleku. Ta metoda se uporablja za mleko in pijače na osnovi kravjega, ovčjega in kozjega mleka. Uporablja se tudi za mleko v prahu po pripravi. Instrument lahko beleži aktivnosti do 7000 milienot na liter (mU/l). Če je aktivnost višja od 7000 mU/l, se razredči z mlekom brez vsebnosti alkalne fosfataze (točka 7.1), da se pridobi meritev, ki ne presega 7000 mU/l.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-januar-2014
1DGRPHãþD
SIST EN ISO 11816-1:2006
0OHNRLQPOHþQLSURL]YRGL'RORþHYDQMHDNWLYQRVWLDONDOQHIRVIDWD]HGHO
)OXRULPHWULMVNDPHWRGD]DPOHNRLQSLMDþHQDRVQRYLPOHND ,62
Milk and milk products - Determination of alkaline phosphatase activity - Part 1:
Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2013)
Milch und Milchprodukte - Bestimmung der Aktivität der alkalischen Phosphatase - Teil 1:
Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816-1:2013)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1:
Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2013)
Ta slovenski standard je istoveten z: EN ISO 11816-1:2013
ICS:
67.100.10 0OHNRLQSUHGHODQLPOHþQL Milk and processed milk
SURL]YRGL products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11816-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2013
ICS 67.100.10 Supersedes EN ISO 11816-1:2006
English Version
Milk and milk products - Determination of alkaline phosphatase
activity - Part 1: Fluorimetric method for milk and milk-based
drinks (ISO 11816-1:2013)
Lait et produits laitiers - Détermination de l'activité de la Milch und Milcherzeugnisse - Bestimmung der Aktivität der
phosphatase alcaline - Partie 1: Méthode fluorimétrique alkalischen Phosphatase - Teil 1: Fluorimetrisches
pour le lait et les boissons à base de lait (ISO 11816- Verfahren für Milch und flüssige Milchprodukte (ISO 11816-
1:2013) 1:2013)
This European Standard was approved by CEN on 14 September 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-1:2013 E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 11816-1:2013) has been prepared by Technical Committee ISO/TC 34 “Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by May 2014, and conflicting national standards shall be withdrawn at the
latest by May 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-1:2006.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11816-1:2013 has been approved by CEN as EN ISO 11816-1:2013 without any modification.
INTERNATIONAL ISO
STANDARD 11816-1
IDF
155-1
Third edition
2013-11-01
Milk and milk products —
Determination of alkaline
phosphatase activity —
Part 1:
Fluorimetric method for milk and
milk-based drinks
Lait et produits laitiers — Détermination de l’activité de la
phosphatase alcaline —
Partie 1: Méthode fluorimétrique pour le lait et les boissons à base de lait
Reference numbers
ISO 11816-1:2013(E)
IDF 155-1:2013(E)
©
ISO and IDF 2013
ISO 11816-1:2013(E)
IDF 155-1:2013(E)
© ISO/IDF 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or
by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written
permission. Permission can be requested from either ISO or IDF at the respective address below or ISO’s member body in the
country of the requester.
ISO copyright office International Dairy Federation
Case postale 56 • CH-1211 Geneva 20 Silver Building • Bd Auguste Reyers 70/B • B-1030 Brussels
Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88
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Published in Switzerland
ii © ISO and IDF 2013 – All rights reserved
ISO 11816-1:2013(E)
IDF 155-1:2013(E)
Contents Page
Foreword .v
1 Scope . 1
2 Terms and definitions . 1
3 Principle . 1
4 Reagents . 1
5 Apparatus . 2
6 Sampling . 3
7 Preparations . 3
7.1 Alkaline phosphatase-free milk . 3
7.2 Preparation of test sample . 4
8 Procedure. 4
8.1 Verification of instrument performance . 4
8.2 Reagent controls to test the suitability of ready-to-use working substrate (4.3) . 5
8.3 Calibration . 5
8.4 Determination . 6
8.5 Test sample-related controls . 6
9 Calculation and expression of results . 7
9.1 Calibration ratio . 7
9.2 Calculation . 8
9.3 Expression of test results . 8
10 Precision . 8
10.1 Collaborative test . 8
10.2 Repeatability . 8
10.3 Reproducibility . 8
11 Test report . 9
Annex A
(informative)
Collaborative trials .10
Bibliography .13
ISO 11816-1:2013(E)
IDF 155-1:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national
standards bodies (ISO member bodies). The work of preparing International Standards is normally
carried out through ISO technical committees. Each member body interested in a subject for which
a technical committee has been established has the right to be represented on that committee.
International organizations, governmental and non-governmental, in liaison with ISO, also take part
in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all
matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directives
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received. www.iso.org/patents
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 5, Milk and
milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO and IDF.
This third edition of ISO 11816-1|IDF 155-1 cancels and replaces the second edition (ISO 11816-1:2006),
which has been technically revised.
ISO 11816|IDF 155 consists of the following parts, under the general title Milk and milk products —
Determination of alkaline phosphatase activity:
— Part 1: Fluorimetric method for milk and milk-based drinks
— Part 2: Fluorimetric method for cheese
iv © ISO and IDF 2013 – All rights reserved
...
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