Plastics - Determination of refractive index (ISO 489:2022)

This document specifies two test methods for determining the refractive index of plastics, namely:
—    Method A: a refractometric method for measuring the refractive index of moulded parts, cast or extruded sheet or film, by means of a refractometer. It is applicable not only to isotropic transparent, translucent, coloured or opaque materials but also to anisotropic materials.
—    Method B: an immersion method (making use of the Becke line phenomenon) for determining the refractive index of powdered or granulated transparent materials by means of a microscope. Monochromatic light, in general, is used to avoid dispersion effects.
NOTE      The refractive index is a fundamental property which can be used for checking purity and composition, for the identification of materials and for the design of optical parts. The change in refractive index with temperature can give an indication of transition points of materials.

Kunststoffe - Bestimmung des Brechungsindex (ISO 489:2022)

Dieses Dokument legt zwei Verfahren zur Bestimmung des Brechungsindex von Kunststoffen fest:
- Verfahren A: Refraktometerverfahren zur Messung des Brechungsindex von Formteilen, gegossenen oder stranggepressten Tafeln oder Folien mit einem Refraktometer. Dieses Verfahren gilt nicht nur für isotrope durchsichtige, durchscheinende, gefärbte oder undurchsichtige, sondern auch für anisotrope Materialien.
- Verfahren B: Immersionsverfahren (unter Ausnutzung des Becke-Linien-Phänomens) zur Bestimmung des Brechungsindex von pulverförmigen oder granulierten durchsichtigen Materialien unter Anwendung eines Mikroskops. Im Allgemeinen sollte monochromatisches Licht verwendet werden, um Dispersionseffekte zu vermeiden.
ANMERKUNG 1 Der Brechungsindex ist eine grundlegende Eigenschaft, die zur Überprüfung der Reinheit und der Zusammensetzung, zur Identifizierung von Werkstoffen und zur Bemessung optischer Teile verwendet werden kann. Aus der Änderung des Brechungsindex mit der Temperatur kann der Übergangspunkt der Werkstoffe ermittelt werden.
ANMERKUNG 2 Die Messgenauigkeit des Verfahrens B entspricht unter der Voraussetzung, dass ein erfahrener Prüfer das Verfahren sehr sorgfältig anwendet, etwa der Messgenauigkeit des Verfahrens A (siehe Abschnitt 8).

Plastiques - Détermination de l’indice de réfraction (ISO 489:2022)

Le présent document spécifie deux méthodes d'essai visant à déterminer l'indice de réfraction des plastiques, à savoir:
—    Méthode A: méthode réfractométrique destinée à mesurer l'indice de réfraction des pièces moulées, se présentant sous forme de feuilles ou de films coulés ou extrudés, au moyen d'un réfractomètre. Elle s'applique non seulement aux matériaux isotropes opaques, colorés, translucides ou transparents, mais aussi aux matériaux anisotropes.
—    Méthode B: méthode d'immersion (utilisation du phénomène de la ligne de Becke) destinée à déterminer l'indice de réfraction de matériaux transparents en poudre ou en granulés à l'aide d'un microscope. La lumière monochromatique est utilisée pour éviter les effets de dispersion.
NOTE         L'indice de réfraction est une propriété fondamentale qui peut être utilisée pour contrôler la pureté et la composition des matières, pour leur identification et pour la conception de pièces d'optique. La variation de l'indice de réfraction suivant la température peut donner une idée du point de transition des matières.

Polimerni materiali - Določanje lomnega količnika (ISO 489:2022)

General Information

Status
Published
Public Enquiry End Date
25-Apr-2021
Publication Date
03-Apr-2022
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
30-Mar-2022
Due Date
04-Jun-2022
Completion Date
04-Apr-2022

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SLOVENSKI STANDARD
SIST EN ISO 489:2022
01-maj-2022
Nadomešča:
SIST EN ISO 489:2000
Polimerni materiali - Določanje lomnega količnika (ISO 489:2022)
Plastics - Determination of refractive index (ISO 489:2022)
Kunststoffe - Bestimmung des Brechungsindex (ISO 489:2022)
Plastiques - Détermination de l’indice de réfraction (ISO 489:2022)
Ta slovenski standard je istoveten z: EN ISO 489:2022
ICS:
83.080.01 Polimerni materiali na Plastics in general
splošno
SIST EN ISO 489:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 489:2022

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SIST EN ISO 489:2022


EN ISO 489
EUROPEAN STANDARD

NORME EUROPÉENNE

March 2022
EUROPÄISCHE NORM
ICS 83.080.01 Supersedes EN ISO 489:1999
English Version

Plastics - Determination of refractive index (ISO 489:2022)
Plastiques - Détermination de l'indice de réfraction Kunststoffe - Bestimmung des Brechungsindex (ISO
(ISO 489:2022) 489:2022)
This European Standard was approved by CEN on 8 February 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 489:2022 E
worldwide for CEN national Members.

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SIST EN ISO 489:2022
EN ISO 489:2022 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 489:2022
EN ISO 489:2022 (E)
European foreword
This document (EN ISO 489:2022) has been prepared by Technical Committee ISO/TC 61 "Plastics" in
collaboration with Technical Committee CEN/TC 249 “Plastics” the secretariat of which is held by NBN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2022, and conflicting national standards
shall be withdrawn at the latest by September 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 489:1999.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 489:2022 has been approved by CEN as EN ISO 489:2022 without any modification.

3

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SIST EN ISO 489:2022

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SIST EN ISO 489:2022
INTERNATIONAL ISO
STANDARD 489
Third edition
2022-03
Plastics — Determination of refractive
index
Plastiques — Détermination de l'indice de réfraction
Reference number
ISO 489:2022(E)
© ISO 2022

---------------------- Page: 7 ----------------------
SIST EN ISO 489:2022
ISO 489:2022(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
  © ISO 2022 – All rights reserved

---------------------- Page: 8 ----------------------
SIST EN ISO 489:2022
ISO 489:2022(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Apparatus and materials .1
4.1 Method A . 1
4.2 Method B . 2
5 Preparation of test specimens . 3
5.1 Method A . 3
5.2 Method B . 3
5.3 Required number of specimens or measurements . 3
6 Conditioning . 4
7 Procedure .4
7.1 Method A . 4
7.1.1 General . 4
7.1.2 Transparent sheet . 4
7.1.3 Film . 6
7.1.4 Anisotropic material . 6
7.1.5 Translucent, coloured and opaque material . 7
7.2 Method B . 8
8 Precision . 9
9 Test report . 9
Annex A (informative) Precision data .10
Bibliography .12
iii
© ISO 2022 – All rights reserved

---------------------- Page: 9 ----------------------
SIST EN ISO 489:2022
ISO 489:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 61, Plastics, Subcommittee SC 5, Physical-
chemical properties, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 249, Plastics, in accordance with the Agreement on technical cooperation between
ISO and CEN (Vienna Agreement).
This third edition cancels and replaces the second edition (ISO 489:1999), which has been technically
revised.
The main changes compared to the previous edition are as follows:
— in the Scope, the description about the precision of the explanation of the method A and method B
has been deleted;
— normative references have been updated;
— the definition of the temperature control device of method A has been changed;
— the text of Clause 8, Precision, has been moved to Annex A;
— in Clause 9, the type of the immersing liquid used in method B has been added;
— the document has been editorial revised.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
  © ISO 2022 – All rights reserved

---------------------- Page: 10 ----------------------
SIST EN ISO 489:2022
INTERNATIONAL STANDARD ISO 489:2022(E)
Plastics — Determination of refractive index
1 Scope
This document specifies two test methods for determining the refractive index of plastics, namely:
— Method A: a refractometric method for measuring the refractive index of moulded parts, cast or
extruded sheet or film, by means of a refractometer. It is applicable not only to isotropic transparent,
translucent, coloured or opaque materials but also to anisotropic materials.
— Method B: an immersion method (making use of the Becke line phenomenon) for determining
the refractive index of powdered or granulated transparent materials by means of a microscope.
Monochromatic light, in general, is used to avoid dispersion effects.
NOTE The refractive index is a fundamental property which can be used for checking purity and
composition, for the identification of materials and for the design of optical parts. The change in refractive index
with temperature can give an indication of transition points of materials.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 291, Plastics — Standard atmospheres for conditioning and testing
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
4 Apparatus and materials
4.1 Method A
4.1.1 Abbe refractometer, or any other refractometer that can be shown to give the same results,
accurate to 0,001 and capable of measuring the refractive index in the range from 1,300 to 1,700. A
temperature-controlling device (4.1.4) shall be provided for the specimens and prisms.
4.1.2 White or sodium lamp, used as a source of light.
4.1.3 Contacting liquid.
WARNING — The contacting liquid may present an environmental hazard during handling,
storage and disposal. It is the responsibility of the user of this document to verify its toxicity and
establish national and regional regulations for safe handling and disposal.
1
© ISO 2022 – All rights reserved

---------------------- Page: 11 ----------------------
SIST EN ISO 489:2022
ISO 489:2022(E)
The contacting liquid shall have a refractive index higher than that of the material to be examined and
shall not soften, attack or dissolve the plastic material. The liquids listed in Table 1 may be used for the
respective plastic materials, but other liquids meeting these requirements may also be used.
Table 1 — Contacting liquids
Plastic material Contacting liquid
Cellulose derivatives Aniseed oil or 1-bromonaphthalene
Fluorine-containing polymers 1-Bromonaphthalene
Urea-formaldehyde Aniseed oil or 1-bromonaphthalene
Phenol-formaldehyde 1-Bromonaphthalene
Polyethylenes 1-Bromonaphthalene
Polyamides 1-Bromonaphthalene
Unsaturated polyester 1-Bromonaphthalene
Polyisobutylene Saturated aqueous solution of zinc chloride made slightly acid
Poly(methyl methacrylate) Saturated aqueous solution of zinc chloride made slightly acid or
1-bromonaphthalene
Polystyrene Saturated potassium mercury(II) iodide solution
Styrene-acrylonitrile copolymers 1-Br
...

SLOVENSKI STANDARD
oSIST prEN ISO 489:2021
01-april-2021
Polimerni materiali - Določanje lomnega količnika (ISO/DIS 489:2021)
Plastics - Determination of refractive index (ISO/DIS 489:2021)
Kunststoffe - Bestimmung des Brechungsindex (ISO/DIS 489:2021)
Plastiques - Détermination de l’indice de réfraction (ISO/DIS 489:2021)
Ta slovenski standard je istoveten z: prEN ISO 489
ICS:
83.080.01 Polimerni materiali na Plastics in general
splošno
oSIST prEN ISO 489:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN ISO 489:2021

---------------------- Page: 2 ----------------------
oSIST prEN ISO 489:2021
DRAFT INTERNATIONAL STANDARD
ISO/DIS 489
ISO/TC 61/SC 5 Secretariat: DIN
Voting begins on: Voting terminates on:
2021-02-01 2021-04-26
Plastics — Determination of refractive index
Plastiques — Détermination de l'indice de réfraction
ICS: 83.080.01
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 489:2021(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2021

---------------------- Page: 3 ----------------------
oSIST prEN ISO 489:2021
ISO/DIS 489:2021(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved

---------------------- Page: 4 ----------------------
oSIST prEN ISO 489:2021
ISO/DIS 489:2021(E)

Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Apparatus and materials. 2
4.1 Method A . 2
4.2 Method B . 3
5 Preparation of test specimens . 3
5.1 Method A . 3
5.2 Method B . 4
5.3 Required number of specimens or measurements. 4
6 Conditioning . 4
7 Procedure. 4
7.1 Method A . 4
7.1.1 General. 4
7.1.2 Transparent sheet . 4
7.1.3 Film . 6
7.1.4 Anisotropic material . 7
7.1.5 Translucent, coloured and opaque material . 8
7.2 Method B . 8
8 Precision . 9
9 Test report .11
© ISO 2021 – All rights reserved iii

---------------------- Page: 5 ----------------------
oSIST prEN ISO 489:2021
ISO/DIS 489:2021(E)

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 61, Plastics, Subcommittee SC 5, Physical-
chemical properties.
This third edition cancels and replaces the second edition (ISO 489:1999), which has been technically
revised.
The main changes compared to the previous edition are as follows:
— delete the description about the precision of the explanation of the method A and method B in
the scope.
— delete the published date of the reference documents
— change the definition of the temperature control device of method A
— As the report items, added the type of the immersing liquid used in method B
— document has been editorial revised.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2021 – All rights reserved

---------------------- Page: 6 ----------------------
oSIST prEN ISO 489:2021
DRAFT INTERNATIONAL STANDARD ISO/DIS 489:2021(E)
Plastics — Determination of refractive index
1 Scope
This document specifies two test methods for determining the refractive index of plastics, namely:
— Method A: a refractometric method for measuring the refractive index of moulded parts, cast or
extruded sheet or film, by means of a refractometer. It is applicable not only to isotropic transparent,
translucent, coloured or opaque materials but also to anisotropic materials.
— Method B: an immersion method (making use of the Becke line phenomenon) for determining
the refractive index of powdered or granulated transparent materials by means of a microscope.
Monochromatic light should, in general, be used to avoid dispersion effects.
NOTE 1 The refractive index is a fundamental property which can be used for checking purity and
composition, for the identification of materials and for the design of optical parts. The change in refractive index
with temperature can give an indication of transition points of materials.
NOTE 2 The accuracy of method B is approximately the same as that of method A when an experienced
operator uses the method with extreme care (see Clause 8).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 291, Plastics — Standard atmospheres for conditioning and testing
ISO 5725-1, Accuracy (trueness and precision) of measurement methods and results — Part 1: General
principles and definitions
ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method
for the determination of repeatability and reproducibility of a standard measurement method
ISO 5725-3, Accuracy (trueness and precision) of measurement methods and results — Part 3: Intermediate
measures of the precision of a standard measurement method
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
© ISO 2021 – All rights reserved 1

---------------------- Page: 7 ----------------------
oSIST prEN ISO 489:2021
ISO/DIS 489:2021(E)

4 Apparatus and materials
4.1 Method A
4.1.1 Abbe refractometer, or any other refractometer that can be shown to give the same results,
reading precision to 0,001 and capable of measuring the refractive index in the range from 1,300 to
1,700. A temperature-controlling device (4.1.4) shall be provided for the specimens and prisms.
4.1.2 White or sodium lamp, used as a source of light.
4.1.3 Contacting liquid.
WARNING — The contacting liquid may present an environmental hazard during handling,
storage and disposal. Verify its toxicity and follow national and regional regulations for safe
handling and disposal.
The contacting liquid shall have a refractive index higher than that of the material to be examined and
shall not soften, attack or dissolve the plastic material. The liquids listed in Table 1 may be used for the
respective plastic materials, but other liquids meeting these requirements may also be used.
Table 1 — Contacting liquids
Plastic material Contacting liquid
Cellulose derivatives Aniseed oil or 1-bromonaphthalene
Fluorine-containing polymers 1-Bromonaphthalene
Urea-formaldehyde Aniseed oil or 1-bromonaphthalene
Phenol-formaldehyde 1-Bromonaphthalene
Polyethylenes 1-Bromonaphthalene
Polyamides 1-Bromonaphthalene
Unsaturated polyester 1-Bromonaphthalene
Polyisobutylene Saturated aqueous solution of zinc chloride made slightly acid
Poly(methyl methacrylate) Saturated aqueous solution of zinc chloride made slightly acid or
1-bromonaphthalene
Polystyrene Saturated potassium mercury(II) iodide solution
Styrene-acrylonitrile copolymers 1-Bromonaphthalene
Vinyl resins (vinyl chloride copolymer or 1-Bromonaphthalene
plasticized PVC)
Poly(vinyl chloride) 1-Bromonaphthalene
Poly(ethylene terephthalate) Methylene iodide
Polycarbonate Methylene iodide
Diethylene glycol bis(allyl carbonate) (CR 39) Methyl salicylate, aniseed oil or 1-bromonaphthalene
Polyarylate Saturated aqueous solution of zinc chloride made slightly acid, meth-
ylene iodide or 1-bromonaphthalene
Polyetheretherketone Methylene iodide
Polypropylene 1-Bromonaphthalene
4.1.4 Temperature control system, capable maintaining the temperature of the main prism, sub-
prism and specimen at (23 ± 0.5) °C.
2 © ISO 2021 – All rights reserved

---------------------- Page: 8 ----------------------
oSIST prEN ISO 489:2021
ISO/DIS 489:2021(E)

4.2 Method B
4.2.1 Microscope, having a magnifying power of at least × 200, an objective giving approximately
× 20 of primary magnification and a substage condenser fitted with a centering illuminating-aperture
diaphragm capable of being stopped down to give a very narrow axial beam.
4.2.2 Monochromatic light, usually the sodium D line, having a wavelength of 589 nm, is used as
the light source for the microscope.
4.2.3 Immersion liquids, with different refractive indices.
WARNING — The contacting liquid may prese
...

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