oSIST prEN ISO 25652:2025

Sediment, Soil, sludge and waste - Analysis of PFAS by HPLC and mass spectrometry (ISO/DIS 25652:2025)

This document specifies a method for quantitative determination of various perfluorinated hydrocarbons by means of High-Performance Liquid Chromatography (HPLC) and mass spectrometry in soil, sludge, sediment and waste (see Table 1).
For many substances to which this document applies a limit of quantification (LOQ) of 0.1 to 10 μg/kgdm can be achieved.
The method can be applied to the analysis of additional PFAS not specified in the scope, if validity is proven by proper in-house validation protocols.
For each target compound both, eventually occurring branched, isomers and the respective non-branched isomer are quantified together. In this method the amount of linear and branched PFAS is quantified using the response factor of the linear PFAS in the calibration standard and the total area of the linear and branched PFAS (Annex 1 gives more explanation)

Boden, Sediment, Schlamm und Abfall - Analyse von PFAS durch HPLC und Massenspektrometrie (ISO/DIS 25652:2025)

Sédiments, sol, boues et déchets - Analyse des PFAS par CLHP et spectrométrie de masse (ISO/DIS 25652:2025)

Tla, usedline, blato in odpadki - Analiza perfluoroalkilnih in polifluoroalkilnih snovi (PFAS) s tekočinsko kromatografijo visoke ločljivosti (HPLC) in masno spektrometrijo (ISO/DIS 25652:2025)

General Information

• Status: Not Published
• Public Enquiry End Date: 02-Jan-2026
• ICS: 13.080.10 - Chemical characteristics of soils
• Technical Committee: KAT - Soil quality
• Current Stage: 4020 - Public enquire (PE) (Adopted Project)
• Start Date: 30-Oct-2025
• Due Date: 19-Mar-2026
• Ref Project: prEN ISO 25652 - Sediment, Soil, sludge and waste - Analysis of PFAS by HPLC and mass spectrometry(ISO/DIS 25652:2025)

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2025
Tla, usedline, blato in odpadki - Analiza perfluoroalkilnih in polifluoroalkilnih snovi
(PFAS) s tekočinsko kromatografijo visoke ločljivosti (HPLC) in masno
spektrometrijo (ISO/DIS 25652:2025)
Sediment, Soil, sludge and waste - Analysis of PFAS by HPLC and mass spectrometry
(ISO/DIS 25652:2025)
Boden, Sediment, Schlamm und Abfall - Analyse von PFAS durch HPLC und
Massenspektrometrie (ISO/DIS 25652:2025)
Sédiments, sol, boues et déchets - Analyse des PFAS par CLHP et spectrométrie de
masse (ISO/DIS 25652:2025)
Ta slovenski standard je istoveten z: prEN ISO 25652
ICS:
13.080.10 Kemijske značilnosti tal Chemical characteristics of
soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
International
Standard
ISO/DIS 25652
ISO/TC 190/SC 3
Sediment, Soil, sludge and waste —
Secretariat: DIN
Analysis of PFAS by HPLC and mass
Voting begins on:
spectrometry
2025-10-27
Les sédiments, le sol, les boues et les déchets — Analyse des PFAS
Voting terminates on:
par HPLC et spectrométrie de masse
2026-01-19
ICS: 13.080.10
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document has not been edited by the ISO Central Secretariat.
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Reference number
ISO/DIS 25652:2025(en)
DRAFT
ISO/DIS 25652:2025(en)
International
Standard
ISO/DIS 25652
ISO/TC 190/SC 3
Sediment, Soil, sludge and waste —
Secretariat: DIN
Analysis of PFAS by HPLC and mass
Voting begins on:
spectrometry
Les sédiments, le sol, les boues et les déchets — Analyse des PFAS
Voting terminates on:
par HPLC et spectrométrie de masse
ICS: 13.080.10
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document has not been edited by the ISO Central Secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2025
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
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WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
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TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
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Published in Switzerland Reference number
ISO/DIS 25652:2025(en)
ii
ISO/DIS 25652:2025(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 5
3 Terms and Definitions . 5
4 Principle . 5
5 Interferences . 5
5.1 General .5
5.2 Interferences in the extraction and cleaning and processing of extracts .5
5.3 Interferences in the HPLC-MS / MS analysis .6
6 Reagents . 6
7 Equipment . 8
8 Sample storage, extraction procedure and analytical analysis preservation . 9
8.1 Sample storage .9
8.2 Sample pretreatment .9
8.3 Extraction .10
8.4 Purification of the extract .10
8.5 HPLC-MS/MS analysis .10
8.6 Chromatographic separation . 13
8.7 Instrumental Detection Limit . 13
8.8 Method blank .14
9 Calibration . 14
9.1 General .14
9.2 Calibration of the method using an internal standard .14
9.3 Concentration calculation based on calibration with internal standards . 15
9.4 Handling of samples outside the scope of the calibration.16
10 Evaluation .16
10.1 Identification .16
10.2 Validity of the calibration .17
10.3 Quantification of branched isomers .17
10.4 Recovery of isotope labelled standards .17
11 Expression of results . 17
Annex A (informative) Flow Chart . 19
Annex B (informative) Example chromatograms .20
Annex C (informative) Examples of Chromatographic Separation of Linear and Branched PFAS .22
Annex D (informative) Validation results .27
Bibliography .42

iii
ISO/DIS 25652:2025(en)
Foreword
This document (prISO 25652:2025) has been prepared by Technical Committee CEN/TC 444 “Environmental
characterization”, in collaboration with Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 3,
Chemical and physical characterization. The secretariat of CEN/TC 444 is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by XXXXXXX, and conflicting national standards shall be withdrawn at
the latest by XXXXXX.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus,
Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany,
Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland and Turkey.

iv
ISO/DIS 25652:2025(en)
Introduction
PFAS is a large group of industrially produced fluorinated hydrocarbons (alkyl substances). PFAS stands for
per- and polyfluoroalkyl substances and contain a fully (per-) or partially (poly-) fluorinated carbon chain.
The length normally ranges from 2 to 18 carbon atoms. The best known PFAS are PFOS (perfluorooctane
1)
sulfonic acid) and PFOA (perfluorooctanoic acid) .
Since 2018, explicit attention has been requested for PFAS in soil trading. Due to the potential risks of the
substance group or the lack of information about it, the target values  for soil trading in some countries are
relatively low at the level of a few µg / kg. That is why in many cases the chemical analyses are decisive
in decision-making for soil trading and determining the possibilities for use of soil and dredged material
released in projects. Precisely because of the diffuse occurrence of PFAS and the relatively strict target
values, there is a generic need for a validated analysis method with a substantiated reporting limit and
uniformity in the analysis methods and the associated pre-treatment.
The need for validation and uniformity does not only affect chemical analysis. The sampling strategy and
method of performing the fieldwork also determine the final result. To this end, various guidelines have
been and will be provided in other contexts and assessment guidelines and protocols have been drawn up.
1) Both substances, PFOS and PFOA, consist of eight and seven fully fluorinated carbon atoms, respectively, with PFOS
additionally having a sulfonate as a "functional group" on the last carbon atom and PFOA a carboxyl group.
...