SIST EN ISO 23753-2:2011

Soil Quality - Determination of dehydrogenase activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2005)

ISO 23753-2:2005 specifies a method for determining soil dehydrogenase activity using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT). As the INT reduction is less sensitive to O2, the method is more reproducible than the TTC-method described in ISO 23753-1. It is not applicable for determining the dehydrogenase activity in upper layers (L, F, H horizons) of forest humus forms with low microbial activity (e.g. mor), or in soils showing reducing properties (e.g. waterlogged soils).

Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2: Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2005)

Diese Internationale Norm legt ein Verfahren zur Bestimmung der Dehydrogenaseaktivität von Böden mit
2-(4-Iodophenyl-)-3(4-nitrophenyl-)-5-phenyltetrazoliumchlorid (INT) fest. Da die INT-Reduktion gegenüber O2
weniger empfindlich ist, ist das Verfahren reproduzierbarer als das in ISO 23753-1 beschriebene TTCVerfahren.
Diese Norm gilt nicht für die Bestimmung der Dehydrogenaseaktivität in Auflagehorizonten (L, F, H) von
Waldhumusformen mit geringer mikrobieller Aktivität (z. B. Moor) oder in Böden mit reduzierenden
Eigenschaften (z. B. staunasse Böden).

Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2: Méthode au chlorure de iodotétrazolium (CIT) (ISO 23753-2:2005)

L'ISO 23753‑2:2005 spécifie une méthode de détermination de l'activité des déshydrogénases dans le sol à l'aide de chlorure de 2‑(4‑iodophényl)‑3‑(4-nitrophényl)-5-phényltétrazolium (CIT). Comme la réduction par le CIT est moins sensible à la présence d'O2, cette méthode est plus reproductible que la méthode au CTT décrite dans l'ISO 23753‑1. Elle ne s'applique pas à la détermination de l'activité des déshydrogénases dans les couches supérieures (horizons L, F et H) des formes d'humus de forêt à faible activité microbienne (par exemple le mor), ou dans les sols présentant des propriétés réductrices (par exemple les sols saturés d'eau).

Kakovost tal - Določevanje aktivnostii dehidrogenaze v tleh - 2. del: Metoda z jodotetrazolijevim kloridom (INT) (ISO 23753-2:2005)

ISO 23753-2:2005 opredeljuje metodo za določevanje aktivnosti dehidrogenaze v tleh z uporabo 2-(4-jodofenil)-3-(4-nitrofenil)-5-feniltetrazolijevega klorida (INT). Ker je redukcija z INT manj občutljiva za O2, je metoda bolj ponovljiva kot metoda s TTC, opisana v ISO 23753-1. Ne velja za določevanje aktivnosti dehidrogenaze v zgornjih plasteh (horizontih L, F, H) gozdnih oblik humusa z nizko mikrobno aktivnostjo (npr. trhlini) ali v tleh z redukcijskimi lastnostmi (tleh, prepojenih z vodo).

General Information

• Status: Withdrawn
• Public Enquiry End Date: 30-Apr-2011
• Publication Date: 17-Oct-2011
• Withdrawal Date: 03-Jun-2019
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: KAT - Soil quality
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 31-May-2019
• Due Date: 23-Jun-2019
• Completion Date: 04-Jun-2019
• Ref Project: EN ISO 23753-2:2011 - Soil Quality - Determination of dehydrogenase activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2005)

Relations

• Replaced By: SIST EN ISO 23753-2:2019 - Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2019)
• Effective Date: 01-Jul-2019
• Replaced By: SIST EN ISO 23753-2:2019 - Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2019)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 23753-2:2011
01-november-2011
.DNRYRVWWDO'RORþHYDQMHDNWLYQRVWLLGHKLGURJHQD]HYWOHKGHO0HWRGD]
MRGRWHWUD]ROLMHYLPNORULGRP,17,62
Soil Quality - Determination of dehydrogenase activity in soils - Part 2: Method using
iodotetrazolium chloride (INT) (ISO 23753-2:2005)
Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2:
Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2005)
Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2:
Méthode au chlorure de iodotétrazolium (CIT) (ISO 23753-2:2005)
Ta slovenski standard je istoveten z: EN ISO 23753-2:2011
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 23753-2:2011 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 23753-2:2011

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SIST EN ISO 23753-2:2011


EUROPEAN STANDARD
EN ISO 23753-2

NORME EUROPÉENNE

EUROPÄISCHE NORM
July 2011
ICS 13.080.30
English Version
Soil Quality - Determination of dehydrogenase activity in soils -
Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-
2:2005)
Qualité du sol - Détermination de l'activité des Bodenbeschaffenheit - Bestimmung der
déshydrogénases dans les sols - Partie 2: Méthode au Dehydrogenaseaktivität in Böden - Teil 2: Verfahren mit
chlorure de iodotétrazolium (CIT) (ISO 23753-2:2005) Iodotetrazoliumchlorid (INT) (ISO 23753-2:2005)
This European Standard was approved by CEN on 17 June 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23753-2:2011: E
worldwide for CEN national Members.

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SIST EN ISO 23753-2:2011
EN ISO 23753-2:2011 (E)
Contents Page
Foreword .3

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SIST EN ISO 23753-2:2011
EN ISO 23753-2:2011 (E)
Foreword
The text of ISO 23753-2:2005 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23753-2:2011 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 23753-2:2005 has been approved by CEN as a EN ISO 23753-2:2011 without any
modification.

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SIST EN ISO 23753-2:2011

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SIST EN ISO 23753-2:2011

INTERNATIONAL ISO
STANDARD 23753-2
First edition
2005-11-01

Soil quality — Determination of
dehydrogenase activity in soils —
Part 2:
Method using iodotetrazolium chloride
(INT)
Qualité du sol — Détermination de l'activité des déshydrogénases dans
les sols —
Partie 2: Méthode au chlorure de iodotétrazolium (CIT)




Reference number
ISO 23753-2:2005(E)
©
ISO 2005

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SIST EN ISO 23753-2:2011
ISO 23753-2:2005(E)
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ii © ISO 2005 – All rights reserved

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SIST EN ISO 23753-2:2011
ISO 23753-2:2005(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Principle. 1
4 Reagents and materials . 2
5 Apparatus . 3
6 Procedure . 3
7 Calculation. 3
8 Test report . 4
Bibliography . 5

© ISO 2005 – All rights reserved iii

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SIST EN ISO 23753-2:2011
ISO 23753-2:2005(E)
Foreword
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rights. ISO shall not be held responsible for identifying any or a
...