SIST EN ISO 18363-3:2022

Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-3:2017)

This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis.
This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 3: Verfahren mittels Säureumesterung und Messung für 2 MCPD, 3 MCPD und Glycidol (ISO 18363 3:2017)

Dieses Dokument legt ein Verfahren für die gleichzeitige Bestimmung von 2 MCPD-Estern (gebundenem 2 MCPD), 3 MCPD-Estern (gebundenem 3 MCPD) und Glycidylestern (gebundenem Glycidol) in einem einzigen Assay fest, basierend auf einer vor der GC/MS-Analyse erfolgenden säurekatalysierten Esterspaltung und Derivatisierung der abgespaltenen (freien) Analyten mit Phenylboronsäure (en: phenylboronic acid, PBA).
Dieses Dokument ist auf feste und flüssige Fette und Öle anwendbar. Für alle drei Analyten liegt die Quantifizierungsgrenze (LOQ, en: limit of quantification) bei 0,1 mg/kg und die Nachweisgrenze (LOD, en: limit of detection) bei 0,03 mg/kg.

Corps gras d'origines animale et végétale - Détermination des esters de chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras par CPG/SM - Partie 3: Méthode par transestérification acide et mesure du 2-MCPD, du 3-MCPD et du glycidol (ISO 18363-3:2017)

ISO 18363-3:2017 spécifie un mode opératoire permettant la détermination simultanée des esters de 2-MCPD (2-MCPD lié), des esters de 3-MCPD (3-MCPD lié) et des esters de glycidol (glycidol lié) en un seul essai, basé sur le clivage des esters par catalyse acide et la dérivatisation des analytes clivés (libres) à l'acide phénylboronique (PBA) avant analyse par CPG/SM.
ISO 18363-3:2017 est applicable aux corps gras solides et liquides. Pour l'ensemble des trois analytes, la limite de quantification (LOQ) est de 0,1 mg/kg et la limite de détection (LOD) est de 0,03 mg/kg.

Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih kloropropandiolov (MCPD) in glicidola z GC/MS - 3. del: Metoda z uporabo kislinske transesterifikacije in meritev 2-MCPD, 3-MCPD in glicidola (ISO 18363-3:2017)

Ta dokument določa postopek za hkratno določevanje estrov 2-MCPD (vezan 2-MCPD), estrov 3-MCPD (vezan 3-MCPD) in glicidil estrov (vezan glicidol) v enem samem testu, ki temelji na cepljenju estrov, kataliziranih s kislino, in derivatizaciji razcepljenih (prostih) analitov s fenilboronsko kislino (PBA) pred analizo GC/MS.
Ta dokument se uporablja za trdne in tekoče maščobe ter olja. Za vse tri analite je meja kvantifikacije (LOQ) 0,1 mg/kg, meja zaznavanja (LOD) pa 0,03 mg/kg.

General Information

• Status: Withdrawn
• Public Enquiry End Date: 28-Oct-2021
• Publication Date: 18-Jan-2022
• Withdrawal Date: 24-Jul-2024
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 25-Jul-2024
• Due Date: 17-Aug-2024
• Completion Date: 25-Jul-2024
• Ref Project: EN ISO 18363-3:2021 - Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-3:2017)

Relations

• Revised: SIST EN ISO 18363-3:2024 - Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-3:2024)
• Effective Date: 06-Dec-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-februar-2022
Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih
kloropropandiolov (MCPD) in glicidola z GC/MS - 3. del: Metoda z uporabo
kislinske transesterifikacije in meritev 2-MCPD, 3-MCPD in glicidola (ISO 18363-
3:2017)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid
transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-
3:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 3: Verfahren mittels
Säureumesterung und Messung für 2 MCPD, 3 MCPD und Glycidol (ISO 18363 3:2017)
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 3: Méthode par transestérification acide et mesure du 2-MCPD, du
3-MCPD et du glycidol (ISO 18363-3:2017)
Ta slovenski standard je istoveten z: EN ISO 18363-3:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18363-3
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2021
EUROPÄISCHE NORM
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Determination of fatty-
acid-bound chloropropanediols (MCPDs) and glycidol by
GC/MS - Part 3: Method using acid transesterification and
measurement for 2-MCPD, 3-MCPD and glycidol (ISO
18363-3:2017)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des esters de chloropropanediols von fettsäuregebundenen Chlorpropandiol (MCPD)
(MCPD) et d'acides gras et des esters de glycidol et und Glycidol mittels GC/MS - Teil 3: Verfahren mittels
d'acides gras par CPG/SM - Partie 3: Méthode par Säureumesterung und Messung für 2 MCPD, 3 MCPD
transestérification acide et mesure du 2-MCPD, du 3- und Glycidol (ISO 18363 3:2017)
MCPD et du glycidol (ISO 18363-3:2017)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18363-3:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 18363-3:2017 has been prepared by Technical Committee ISO/TC 34 "Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 18363-
3:2021 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18363-3:2017 has been approved by CEN as EN ISO 18363-3:2021 without any
modification.
INTERNATIONAL ISO
STANDARD 18363-3
First edition
2017-12
Animal and vegetable fats and oils —
Determination of fatty-acid-bound
chloropropanediols (MCPDs) and
glycidol by GC/MS —
Part 3:
Method using acid transesterification
and measurement for 2-MCPD,
3-MCPD and glycidol
Corps gras d'origines animale et végétale — Détermination des
esters de chloropropanediols (MCPD) et d'acides gras et des esters de
glycidol et d'acides gras par CPG/SM —
Partie 3: Méthode par transestérification acide et mesure pour le
2-MCPD, le 3-MCPD et le glycidol
Reference number
ISO 18363-3:2017(E)
©
ISO 2017
ISO 18363-3:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
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ii © ISO 2017 – All rights reserved

ISO 18363-3:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
5.1 Standard and reference compounds . 2
5.2 Standard solutions . 3
5.2.1 General. 3
5.2.2 Stock solutions (1 mg/ml) . 3
5.2.3 Working solutions . 3
5.3 Other reagents . 4
5.4 Reagent solutions . 4
6 Apparatus . 5
7 Sample . 5
7.1 Sampling . 5
7.2 Preparation of the test sample . 5
8 Procedure. 6
8.1 Test sample preparation . 6
8.2 Preparation of the calibration curve . 7
8.3 Gas chromatography/mass spectrometry references . 7
9 Expression of results . 8
9.1 Quantification of 3-MCPD esters . 8
9.2 Quantification of 2-MCPD esters . 9
9.3 Quantification of glycidyl esters . 9
10 Precision .10
10.1 General .10
10.2 Repeatability .10
10.3 Between-day reproducibility .10
11 Test report .11
Annex A (informative) Construction of the calibration curves .12
Annex B (informative) Results of the interlaboratory test .16
Bibliography .18
ISO 18363-3:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
c
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