SIST EN 17719:2025
(Main)Plant biostimulants - Determination of the anaerobic plate count
Plant biostimulants - Determination of the anaerobic plate count
This standard was developed to provide a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after anaerobic incubation at 30 °C, thereby verifying that they are not present in plant biostimulants (if the biostimulant itself is not anaerobic bacteria).
Pflanzen-Biostimulanzien - Bestimmung der anaeroben Keimzahl
Dieses Dokument stellt ein horizontales Verfahren zur Zählung von Mikroorganismen, die nach anaerober Bebrütung bei 30 °C in einem festen Medium wachsen und Kolonien bilden können, zur Verfügung.
Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, ausgenommen jene, die aus aeroben Bakterien bestehen, um zu überprüfen, ob die Konzentration der Anaerobier die entsprechenden Grenzwerte der EU Düngeprodukteverordnung [1] nicht überschreitet.
Dieses Verfahren ist nicht anzuwenden für die mikrobiologische Überwachung der Umgebung, in der die mikrobiellen Pflanzen-Biostimulanzien hergestellt werden.
Es können keine Informationen über potentielle humanpathogene Keime aus der Auszählung der anaeroben koloniebildenden Einheiten abgeleitet werden.
Biostimulants des végétaux - Détermination du dénombrement sur plaque des germes anaérobies
Le présent document fournit une méthode horizontale pour le dénombrement des microorganismes qui sont capables de croître et de former des colonies dans un milieu solide après une incubation anaérobie à 30 °C.
La méthode est applicable aux biostimulants microbiens des végétaux, sauf ceux composés de bactéries aérobies, pour vérifier que la concentration en anaérobies ne dépasse pas les limites correspondantes indiquées dans le Règlement UE sur les fertilisants [1].
La présente méthode ne s’applique pas à la surveillance microbiologique de l’environnement dans lequel les biostimulants microbiens des végétaux sont fabriqués.
Aucune information sur les pathogènes humains potentiels ne peut être déduite des dénombrements sur plaque des germes anaérobies.
Rastlinski biostimulanti - Ugotavljanje števila anaerobnih mikroorganizmov na mikrotitrskih ploščah
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17719:2023
Rastlinski biostimulanti - Ugotavljanje števila anaerobnih mikroorganizmov na
mikrotitrskih ploščah
Plant biostimulants - Determination of the anaerobic plate count
Pflanzen-Biostimulanzien - Bestimmung der anaeroben Keimzahl
Biostimulants des végétaux - Détermination du dénombrement sur plaque des germes
anaérobies
Ta slovenski standard je istoveten z: EN 17719:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17719
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17719:2022
English Version
Plant biostimulants - Determination of the anaerobic plate
count
Biostimulants des végétaux - Détermination du Pflanzen-Biostimulanzien - Bestimmung der anaeroben
dénombrement sur plaque des germes anaérobies Keimzahl
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17719:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Culture media and reagents . 6
6 Equipment and consumables . 6
7 Preparation of test sample . 7
8 Procedure . 8
9 Calculation . 9
10 Expression of results . 9
11 Performance characteristics of the method . 9
12 Test report . 10
13 Quality assurance . 10
Annex A (informative) Guidelines for calculating and reporting the anaerobic plate counts
(ANPC) . 11
Annex B (normative) Composition and preparation of culture media and reagents . 12
Annex C (informative) Results of the interlaboratory study for the determination of the
anaerobic plate count . 15
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 17
Bibliography . 18
European foreword
This document (EN 17719:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17719:2022.
— The European foreword and the introduction have been updated;
— The Normative references reported in Clause 2 have been updated;
— The definitions have been revised;
— Clauses 7, 8, and 10 have been revised;
— Annexes A, and B have been revised;
— Annex C on the results of the interlaboratory study for the determination of the anaerobic plate count
has been added;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document specifies a horizontal method for the enumeration of microorganisms that are able to
grow and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method applies to microbial plant biostimulants, except those composed of aerobic bacteria.
This method does not apply to the microbiological monitoring of the environment in which microbial
plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Plant Biostimulants and where the following category Plant Biostimulants is the highest
percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant
Biostimulants is not the highest percentage in the blend, the European Standard for the highest
percentage of the blend applies. In case a blend of fertilizing products is composed of components in equal
quantity or in case the component EU fertilising products used for the blend have identical formulations ,
the user decides which standard to apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) shall
apply.
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
EN 17724:2024, Plant biostimulants — Terminology
EN 17702-1:2024, Plant biostimulants —Sampling and sample preparation: Part 1: Sampling
EN 17714:2024, Plant biostimulants — Determination of microorganisms’ concentration
EN ISO 7218:2024, Microbiology of food and animal feeding stuffs — General requirements and guidance
for microbiological examinations (ISO 7218:2024)
EN ISO 11133:2014 , Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media (ISO 11133:2014)
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 17724:2024 and the following
apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
• ISO Online browsing platform: available at https://www.iso.org/obp
An example of such a blend is a product with 2 claimed functions consisting of a non-microbial plant biostimulant
and an organic fertilizer composed of 1 kg/kg of plant biostimulant from seaweed.”
As amended by EN ISO 11133:2014/A1:2018 and EN ISO 11133:2014/A2:2020.
• IEC Electropedia: available at https://www.electropedia.org/
3.1
microorganism
any microbiological entity, including lower fungi, bacteria, and viruses, cellular or non-cellular, capable
of replication or of transferring genetic material
[SOURCE: Regulation (EC) No 1107/2009, Article 3, point 15]
3.2
anaerobic bacteria
any microorganism capable of both aerobic and anaerobic metabolism or that only lives and grows in the
absence of molecular oxygen
[SOURCE: EN ISO 11139:2018, 3.114 modified]
[SOURCE: EN ISO 11139:2018, 3.186 modified]
3.3
plate count
value established from the enumeration of recoverable colony-forming units (CFU)
[SOURCE: EN ISO 11139:2018, 3.316 modified]
4 Principle
4.1 General
Viable anaerobic bacteria shall be enumerated by the plate count technique under an anaerobic
atmosphere [2]. Caution shall be exercised when applying the method since isolates may be pathogenic.
4.2 Brief description
A fixed amount of a dilution of the test sample shall be placed in an empty Petri dish and mixed in a
specified melted agar culture medium to create a pour plate.
The plates shall be incubated at 30 °C ± 1 °C under anaerobic conditions for 48 h to 72 h.
The number of microorganisms per gram or millilitre of test sample shall be calculated as specified in
Clause 9.
5 Culture media and reagents
For current laboratory practice, refer to EN 17708:2024 and EN ISO 11133:2014 .
The composition of Anaerobe Agar (ANA) [3] and reagents, as well as their preparation, are described in
Annex B.
6 Equipment and consumables
6.1 Special apparatus, anaerobic incubator, BBL GasPak or equivalent, equipped with GasPak
hydrogen and CO generator envelopes with an anaerobic indicator.
6.2 Apparatus for dry sterilization (oven) or wet sterilization (autoclave), according to
EN ISO 7218:2024.
...
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