SIST EN 12014-2:2018
(Main)Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products
Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products
This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable products. This method is applicable for samples with a content of 25 mg/kg or greater.
It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and 222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.
Lebensmittel - Bestimmung des Nitrat- und/oder Nitritgehaltes - Teil 2: HPLC/IC-Verfahren für die Bestimmung des Nitratgehaltes in Gemüse und Gemüseerzeugnissen
Diese Europäische Norm legt ein hochleistungs-flüssigchromatographisches (HPLC) und ein ionenchromatographisches (IC) Verfahren zur Bestimmung des Nitratgehaltes in Gemüse und Gemüseerzeugnissen fest. Dieses Verfahren ist auf Proben mit einem Gehalt von 25 mg/kg oder größer anwendbar.
Es wurde mit natürlich kontaminierten und aufgestockten Proben in Rote-Beete-Saft mit Nitrat-Massenanteilen von 194 mg/kg und 691 mg/kg, auf pürierte Karotten mit Nitrat-Massenanteilen von 26 mg/kg und 222 mg/kg sowie auf Eisbergsalat mit Nitrat-Massenanteilen von 623 mg/kg und 3 542 mg/kg validiert.
Produits alimentaires - Détermination de la teneur en nitrates et/ou en nitrites - Partie 2: Méthode de détermination par CLHP/CI de la teneur en nitrates des légumes et des produits à base de légumes
La présente Norme européenne spécifie une méthode de chromatographie en phase liquide à haute performance (CLHP) et une méthode de chromatographie par échange d'ions (Cl) pour déterminer la teneur en nitrates des légumes et des produits à base de légumes. Ces méthodes sont applicables aux échantillons dont la teneur est supérieure ou égale à 25 mg/kg.
Elles ont été validées sur des échantillons dopés et sur des échantillons contaminés naturellement de jus de betterave ayant une teneur en nitrates (fraction massique) de 194 mg/kg et 691 mg/kg, de purée de carottes ayant une teneur en nitrates (fraction massique) de 26 mg/kg et 222 mg/kg, et de laitue iceberg ayant une teneur en nitrates (fraction massique) de 623 mg/kg et 3 542 mg/kg.
Živila - Določevanje vsebnosti nitratov in/ali nitritov - 2. del: Metoda HPLC/IC za določevanje vsebnosti nitratov v zelenjavi in zelenjavnih proizvodih
Ta evropski standard določa metodo tekočinske kromatografije visoke ločljivosti (HPLC) in ionsko kromatografsko metodo (IC) za določevanje ravni nitrata v zelenjavi in zelenjavnih proizvodih. Ta metoda se uporablja za vzorce z vsebnostjo 25 mg/kg ali več.
Metoda je bila potrjena na naravno kontaminiranih in primešanih vzorcih, na primer soku rdeče pese z masnimi deleži nitrata 194 mg/kg in 691 mg/kg, korenjevim pirejem z masnimi deleži nitrata 26 mg/kg in 222 mg/kg ter ledenki z masnimi deleži nitrata 623 mg/kg in 3542 mg/kg.
General Information
Relations
Overview
EN 12014-2:2017 (CEN) specifies a validated HPLC/IC method for the determination of nitrate content in vegetables and vegetable products. The method applies to samples with nitrate concentrations of 25 mg/kg or greater and was approved by CEN in December 2017. It combines hot‑water extraction with either reverse‑phase HPLC (UV detection) or ion chromatography (conductivity or UV detection) and includes guidance on sample preparation, calibration and evaluation.
Key validated matrices include:
- Beetroot juice (194 mg/kg and 691 mg/kg)
- Pureed carrots (26 mg/kg and 222 mg/kg)
- Iceberg lettuce (623 mg/kg and 3 542 mg/kg)
Key topics and technical requirements
- Scope & applicability: Targeted at vegetables and derived products, minimum reporting level 25 mg/kg.
- Principle: Hot‑water extraction of nitrate from homogenized sample followed by HPLC or IC separation and detection.
- Sample preparation:
- Liquid samples: mix and, if needed, filter or predilute.
- Solid samples: remove contaminants, shred, homogenize; analyze immediately or freeze (storage guidance included).
- Pasty samples: homogenize thoroughly.
- Hot‑water extraction:
10 g sample, ~400 ml hot water (80 °C), boil 15 min, cool, dilute to volume and filter.
- Reagents & standards: Nitrate stock (1 000 mg/l as NO3‑), working standards (typically 1–50 mg/l), potassium/sodium nitrate, nitrate‑free water compliant with ISO 3696.
- Instrumentation:
- HPLC: reversed‑phase columns, UV detector (190–800 nm), pre‑column recommended.
- IC: ion‑exchange columns, conductivity or UV detector, suppressor or electrolytic suppressor options.
- Membrane filtration (0.45 µm regenerated cellulose) for final sample clean‑up.
- Calibration & evaluation: Multi‑level calibration (generally five levels + blank) using peak area/height; dilution and reanalysis when sample response falls outside calibration range.
- Performance data: Includes precision (repeatability and reproducibility) and chromatogram examples in informative annexes.
Applications & who uses it
- Food control and public health laboratories performing regulatory compliance testing for nitrate in vegetables.
- QA/QC teams in food processing and fresh‑produce industries monitoring product safety and labeling.
- Research laboratories studying nitrate exposure, agronomy, and processing effects on vegetable nitrate levels.
- Regulatory agencies implementing limits or surveillance under European food safety legislation.
Related standards
- ISO 3696 - Water for analytical laboratory use (referenced for nitrate‑free water).
- EN 12014 series (this document is Part 2 - nitrate determination by HPLC/IC).
- Commission regulations referenced for sampling practice (e.g., sampling provisions cited in the standard).
This standard is essential for reliable, reproducible measurement of nitrate in vegetables using HPLC or IC and supports regulatory and quality assurance activities across the food sector.
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2018
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SIST EN 12014-2:1999
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Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for
the determination of nitrate content of vegetables and vegetable products
Lebensmittel - Bestimmung des Nitrat- und/oder Nitritgehaltes - Teil 2: HPLC/IC-
Verfahren für die Bestimmung des Nitratgehaltes in Gemüse und Gemüseerzeugnissen
Produits alimentaires - Détermination de la teneur en nitrates et/ou en nitrites - Partie 2:
Méthode de détermination par CLHP/CI de la teneur en nitrates des légumes et des
produits à base de légumes
Ta slovenski standard je istoveten z: EN 12014-2:2017
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.080.20 Zelenjava in zelenjavni Vegetables and derived
proizvodi products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 12014-2
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2017
EUROPÄISCHE NORM
ICS 67.080.20 Supersedes EN 12014-2:1997
English Version
Foodstuffs - Determination of nitrate and/or nitrite
content - Part 2: HPLC/IC method for the determination of
nitrate content of vegetables and vegetable products
Produits alimentaires - Détermination de la teneur en Lebensmittel - Bestimmung des Nitrat- und/oder
nitrates et/ou en nitrites - Partie 2: Méthode de Nitritgehaltes - Teil 2: HPLC/IC-Verfahren für die
détermination par CLHP/CI de la teneur en nitrates des Bestimmung des Nitratgehaltes in Gemüse und
légumes et des produits à base de légumes Gemüseerzeugnissen
This European Standard was approved by CEN on 15 October 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 12014-2:2017 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Principle . 5
4 Reagents . 5
5 Apparatus and equipment . 6
6 Procedure. 7
6.1 Sample preparation . 7
6.1.1 Liquid samples, e.g. vegetable juice . 7
6.1.2 Solid samples, e.g. leaf vegetable . 7
6.1.3 Pasty samples, e.g. mashed vegetables . 7
6.2 Hot water extraction of samples (solid and pasty samples). 7
6.3 Preparation of sample test solutions . 7
6.4 Preparation of the calibration curve. 7
6.5 Determination . 8
6.5.1 High-performance liquid chromatography . 8
6.5.2 Ion chromatography . 8
7 Evaluation . 8
7.1 Identification and quantitative determination . 8
7.2 Calculation . 8
7.3 Precision . 8
7.3.1 General . 8
7.3.2 Repeatability . 9
7.3.3 Reproducibility . 9
8 Test report . 9
Annex A (informative) Examples of HPLC/IC chromatograms . 10
Annex B (informative) Precision data. 13
Annex C (informative) Examples of suitable HPLC conditions . 15
Annex D (informative) Examples of suitable IC conditions . 16
Bibliography . 18
European foreword
This document (EN 12014-2:2017) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2018, and conflicting national standards shall be
withdrawn at the latest by June 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 12014-2:1997.
The following changes have been made to the former edition:
a) the scope has been reduced from 50 mg/kg nitrate to 25 mg/kg nitrate and the upper limit has
been deleted;
b) the purification method 1 and 2 used in the preparation of sample test solutions has been deleted;
c) a new matrix (iceberg lettuce) has been verified in an interlaboratory test;
d) update of the HPLC/IC-conditions and chromatograms in Annex A;
e) the procedure has been extensively revalidated and precision data in Annex B have been revised;
f) editorially revised.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Introduction
The method described in this standard has been developed and validated for investigations regarding
the European legislation for nitrate in vegetables and vegetable products. Laboratory experience has
shown that this analytical method is also suitable for the determination of nitrite in other matrices;
however, this has not been validated in the interlaboratory test scheme cited here.
1 Scope
This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion
chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable
products. This method is applicable for samples with a content of 25 mg/kg or greater.
It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass
fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and
222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Principle
The nitrate is extracted from the foodstuff with hot water. The determination is performed either by
reverse-phase HPLC and UV detection, or by IC and conductivity or UV detection.
4 Reagents
Use only reagents of recognized analytical grade, unless otherwise specified. Water shall be nitrate-free
and shall comply with grade 1 of ISO 3696.
4.1 Potassium nitrate.
4.2 Sodium nitrate.
1)
4.3 Sulfuric acid, 96 % (ρ = 1,84 g/ml ).
2)
4.4 Regenerator for suppressor, e.g. sulfuric acid (c = 0,0125 mol/l ).
Carefully pipette 20 ml of sulfuric acid (4.3) into a 1 000 ml volumetric flask containing 800 ml of water.
Mix the solution, dilute to the mark with water and mix again. Transfer 33 ml of this solution to a
1 000 ml volumetric flask containing 500 ml of water. Dilute to the mark with water and mix.
Alternatively, use a method with electrolytic suppressor.
-
4.5 Nitrate stock solution, ρ = 1 000 mg/l (expressed as ion NO ).
Weigh 1,630 7 g of potassium nitrate (4.1) to the nearest 0,1 mg and dissolve in water in a 1 000 ml
volumetric flask.
Alternatively, 1,370 9 g of sodium nitrate (4.2) can be used. If required, 15 ml of sulfuric acid (4.3) can
be added as a preservative. Mix the solution, dilute to the mark with water and mix again. This solution
is stable for at least two months if stored at +4 °C in the dark (refrigerator).
Alternatively, commercially available standard solutions can also be used.
1) ρ = mass concentration
2) c = substance concentration
4.6 Nitrate standard solutions.
Prepare suitable dilutions from the nitrate stock solution (4.5), preferably in the range of 1 mg/l to
50 mg/l. Pipette an appropriate volume of nitrate stock solution into a 100 ml volumetric flask, dilute to
the mark with water, mix and filter through a membrane filter (5.4), if required.
4.7 Mobile phase, for the HPLC.
Examples of suitable eluents are given in Annex C.
4.8 Mobile phase, for the IC.
Examples of suitable eluents are given in Annex D.
5 Apparatus and equipment
Usual laboratory equipment and, in particular, the following:
5.1 Commercially available laboratory mixer, e.g. a laboratory cutter.
5.2 Homogenizer.
5.3 Fluted filter paper (nitrogen-deficient).
5.4 Membrane filter, for aqueous solutions, with a pore size of 0,45 µm (e.g. regenerated cellulose).
5.5 Filter holder for membrane filter, with suitable syringe.
5.6 HPLC equipment.
5.6.1 High-performance liquid chromatograph, consisting of an eluent reservoir, a pump, a sample
applicator, a UV detector adjustable from 190 nm to 800 nm and an evaluation unit.
5.6.2 Analytical (reversed-phase) separating column.
Examples of suitable separating columns are given in Annex C.
Use a pre-column with filling material of the same type to protect the analytical separating column.
5.7 IC apparatus.
5.7.1 Ion-exchange chromatography unit, consisting of an eluent reservoir, a pump, a pulsation
damper, a dialysis unit (if required), a suppressor (if required), a sample applicator with a sample loop,
a conductivity detector or UV detector adjustable from 190 nm to 800 nm and an evaluation unit.
5.7.2 Analytical separating column.
Examples of suitable separating columns are given in Annex D.
Use a pre-column with filling material of the same type to protect the analytical separating column.
6 Procedure
6.1 Sample preparation
6.1.1 Liquid samples, e.g. vegetable juice
Vigorously shake a representative sample until it is thoroughly mixed. If required, filter the sample
through a fluted filter paper (5.3) or, if necessary, predilute it with water to achieve a nitrate
concentration of approximately 25 mg/l in the solution.
6.1.2 Solid samples, e.g. leaf vegetable
Take a representative sample and remove adhering animals, soil or dirty particles. If required, remove
any inedible or damaged external leaves. Shred the sample prepared in this way, e.g. in a laboratory
cutter (5.1) and thoroughly homogenize it using a homogenizer (5.2). Analyse the sample immediately
after homogenization.
3)
The requirements of the European legislation shall be taken into account, e.g. [1], [2] . If immediate
testing of the sample homogenate is not possible, immediately freeze the sample. For analysis, thaw the
sample as gently as possible (e.g. overnight in the refrigerator).
If it is not possible to homogenize a fresh produced sample within 24 h, the whole sample should be
frozen and analysed within a maximum of 6 weeks.
6.1.3 Pasty samples, e.g. mashed vegetables
Repeatedly stir a representative sample, e.g. by using a ho
...
Frequently Asked Questions
SIST EN 12014-2:2018 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products". This standard covers: This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable products. This method is applicable for samples with a content of 25 mg/kg or greater. It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and 222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.
This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable products. This method is applicable for samples with a content of 25 mg/kg or greater. It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and 222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.
SIST EN 12014-2:2018 is classified under the following ICS (International Classification for Standards) categories: 67.050 - General methods of tests and analysis for food products; 67.080.20 - Vegetables and derived products. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN 12014-2:2018 has the following relationships with other standards: It is inter standard links to SIST EN 12014-2:1999. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN 12014-2:2018 is associated with the following European legislation: EU Directives/Regulations: 882/2004. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
You can purchase SIST EN 12014-2:2018 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
Die SIST EN 12014-2:2018 stellt einen bedeutenden Standard für die Bestimmung des Nitratgehalts in Lebensmitteln, insbesondere in Gemüse und Gemüseprodukten, dar. Dieser Standard beschreibt Methoden für die Hochleistungsflüssigkeitschromatographie (HPLC) und die Ionenchromatographie (IC), wodurch eine präzise Analyse des Nitratgehalts ermöglicht wird. Die Anwendbarkeit des Standards bei Proben mit einem Nitratgehalt von 25 mg/kg oder mehr zeigt die Relevanz für die Lebensmittelindustrie, insbesondere für Produzenten, die sicherstellen möchten, dass ihre Produkte den Sicherheitsstandards entsprechen. Ein wichtiger Stärke dieses Standards liegt in der Validierung der Methoden an verschiedenen Proben, die sowohl natürlich kontaminiert als auch mit definierten Nitrathalten angereichert waren. Diese Validierung reicht von Rote-Bete-Säften mit Nitratschwankungen von 194 mg/kg bis 691 mg/kg, pürierten Karotten mit Nitratgehalten von 26 mg/kg bis 222 mg/kg und Kopf- oder Eisbergsalat mit Werten von 623 mg/kg bis zu 3.542 mg/kg. Die umfassende Erprobung dieser Methodiken stellt sicher, dass sie in der Praxis zuverlässig und genau sind. Die Relevanz der SIST EN 12014-2:2018 wird weiter durch die zunehmende Bedeutung von Lebensmittelsicherheit und gesundheitsbewusster Ernährung unterstrichen. Der Standard bietet eine fundierte Grundlage für die Kontrolle des Nitratgehalts in Gemüseprodukten, was sowohl für Produzenten als auch für Verbraucher von entscheidender Bedeutung ist. Durch die Anwendung dieser standardisierten Methoden können Unternehmen die Qualität und Sicherheit ihrer Produkte garantieren und sich gleichzeitig an gesetzliche Vorgaben halten. Insgesamt trifft die SIST EN 12014-2:2018 die notwendige Balance zwischen wissenschaftlicher Präzision und praktischer Anwendbarkeit, was sie zu einem unerlässlichen Instrument in der Lebensmittelanalytik macht.
SIST EN 12014-2:2018 표준은 식품의 질을 보장하고 안전성을 높이는 데 매우 중요한 역할을 합니다. 이 표준은 고성능 액체 크로마토그래피(HPLC) 및 이온 크로마토그래피(IC) 방법을 통해 채소 및 채소 제품의 질산염 농도를 결정하는 규정을 명확히 합니다. 표준의 적용 범위는 25 mg/kg 이상의 질산염 함량을 가진 샘플에 해당하며, 이는 신뢰성 있는 결과를 제공하는 데 기여합니다. 이 표준의 강점 중 하나는 이론적 배경과 실용성 모두를 갖춘 방법론을 제시한다는 점입니다. 표준은 자연적으로 오염된 샘플과 스파이크 샘플에서 검증을 완료하였으며, 비트주스, 퓌레 당근 및 얼리게이트 상추와 같은 다양한 식품에서 질산염의 질량 분율을 기준으로 하고 있습니다. 이로 인해 다양한 농산물의 품질 관리에 유용하게 적용할 수 있습니다. 또한, SIST EN 12014-2:2018 표준은 식품 산업에서 질산염과 니트라이트의 함량을 정확하게 측정하는 데 필수적인 표준으로 자리잡고 있습니다. 특히, 채소 제품의 안전성을 평가하는 데 있어 이 표준은 공통된 프레임워크를 제공하여 다양한 국가와 지역에서 일관되게 적용될 수 있는 기반을 마련합니다. 이러한 점에서 표준의 관련성과 중요성은 더욱 부각됩니다.
La norme SIST EN 12014-2:2018 constitue un document essentiel dans le domaine de l'analyse des aliments, spécifiquement pour la détermination de la teneur en nitrates dans les légumes et produits dérivés. Cette norme s'appuie sur des méthodes de chromatographie liquide à haute performance (HPLC) et de chromatographie ionique (IC), offrant ainsi une approche robuste et fiable pour quantifier les niveaux de nitrates. L'un des principaux atouts de cette norme est son large champ d'application, car elle s'applique à des échantillons contenant un minimum de 25 mg/kg de nitrates. Cela permet aux laboratoires d'effectuer des analyses précises sur une variété de légumes, y compris des jus de betterave, des carottes en purée et de la laitue iceberg, avec des fractions massiques de nitrates allant jusqu'à 3 542 mg/kg. Cela démontre non seulement la sensibilité des méthodes proposées, mais aussi leur pertinence dans le contrôle de la qualité alimentaire. De plus, la validation de ces méthodes sur des échantillons contaminés naturellement et sur des échantillons spiqués renforce la crédibilité de la norme. Les résultats obtenus garantissent que les valeurs mesurées reflètent fidèlement la réalité, ce qui est crucial pour la conformité aux réglementations en matière de sécurité alimentaire et de santé publique. En somme, la norme SIST EN 12014-2:2018 est d'une grande pertinence pour les analysts, les producteurs et les organismes de réglementation, car elle fournit un cadre méthodologique rigoureux pour évaluer la sécurité des légumes et contribuer à la protection des consommateurs face à des niveaux potentiellement dangereux de nitrates. Sa contribution au secteur de la sécurité alimentaire souligne l'importance d'un contrôle systématique et précis de la composition des aliments.
The SIST EN 12014-2:2018 standard outlines a comprehensive methodology for the determination of nitrate content in vegetables and vegetable products using high-performance liquid chromatography (HPLC) and ion chromatography (IC). Its defined scope enhances the relevance of the standard, as it specifically targets samples exhibiting nitrate levels of 25 mg/kg or higher, ensuring that the method is applied effectively to relevant agricultural produce. One of the notable strengths of this standard lies in its validation process. The method has been rigorously assessed on both naturally contaminated and spiked samples, including beetroot juice, pureed carrots, and iceberg lettuce. This broad validation ensures reliability and accuracy across different vegetable matrices, offering confidence to food safety professionals and laboratories in their assessments of nitrate levels. Furthermore, the specific concentration ranges for the validation-such as 194 mg/kg and 691 mg/kg for beetroot juice-illustrate the method’s efficacy in varying contexts, thus showcasing its versatility. The combination of HPLC and IC methods provides a robust dual approach for determining nitrate content, which is critical given the importance of monitoring nitrate levels for health and safety concerns related to food products. In summary, SIST EN 12014-2:2018 presents a well-defined, validated approach for the determination of nitrate content in vegetables and vegetable products, making it an essential resource for industry professionals focused on maintaining food quality and safety. Its strong scientific basis, wide applicability, and thorough validation underscore its significant relevance in the field of food safety and analytical chemistry.
SIST EN 12014-2:2018は、食品中の硝酸塩および亜硝酸塩の含有量を測定するための重要な標準であり、その中でも特に野菜と野菜製品における硝酸塩の分析に焦点を当てています。この標準は、高速液体クロマトグラフィー（HPLC）およびイオンクロマトグラフィー（IC）を用いた手法を明確に定義しており、これにより、硝酸塩の定量が高い精度で行えることを強調しています。 この規格の範囲は、25 mg/kg以上の硝酸塩含有量を有するサンプルに適用されるため、非常に広範囲に対応できます。そのため、さまざまな野菜製品において、非自然汚染およびスパイクサンプルに対しても検証済みであり、ビートルートジュース、ピューレ状の人参、レタスといった具体的な例を挙げて成果を示しています。これによって、異なる濃度範囲における硝酸塩の測定精度が確保されており、科学的な信頼性を強化しています。 さらに、SIST EN 12014-2:2018は、食品安全規制の枠組み内での重要度も非常に高く、食品業界全体に貢献しています。この標準の採用は、食品の品質管理や安全性評価の一環として、特に現代の健康志向の消費者に対しても意義があります。したがって、この文書は、食品科学研究や業界実務における基準としての重要性を持ち、食品分析技術の進歩に寄与しています。
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