SIST EN 14407:2014
(Main)Water quality - Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes
Water quality - Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes
This European Standard specifies methods for the identification and enumeration of relative proportions of diatom taxa on prepared slides and of data interpretation relevant to assessments of water quality in rivers and lakes. It is suitable for use with indices and assessment methods based on the relative abundance of taxa. The methods for identification and enumeration may also be applied to the study of benthic diatoms in other habitats provided that data interpretation methods appropriate to these habitats are used.
Wasserbeschaffenheit - Anleitung zur Bestimmung und Zählung von benthischen Kieselalgen in Fließgewässern und Seen
Dieses Dokument legt Verfahren zur Identifizierung und Auszählung von relativen Anteilen der Diatomeentaxa auf vorbereiteten Objektträgern und die für die Beurteilung der Wasserbeschaffenheit von Fließgewässern und Seen maßgebliche Datenauswertung fest. Es ist geeignet für die Arbeit mit Indizes und Schätzverfahren, die auf der relativen Häufigkeitsverteilung taxonomischer Einheiten beruhen. Die Verfahren zur Identifizierung und Quantifizierung dürfen auch für Untersuchungen von benthischen Diatomeen in anderen Lebensräumen verwendet werden, vorausgesetzt, dass dafür geeignete Datenauswertungsverfahren verwendet werden.
Qualité de l'eau - Guide pour l'identification et le dénombrement des échantillons de diatomées benthiques de rivières et de lacs
La présente Norme européenne spécifie des méthodes permettant d’identifier et de calculer les proportions relatives des taxons de diatomées sur des lames d’observation et d’interpréter les résultats dans le cadre d’évaluations de la qualité de l’eau des rivières et des lacs. Elle peut être utilisée avec des indices et des méthodes d’évaluation fondés sur l’abondance relative des taxons. Ces méthodes d’identification et de dénombrement peuvent aussi être appliquées à l’étude des diatomées benthiques dans d’autres habitats sous réserve d’utiliser des méthodes d’interprétation des résultats appropriées aux habitats considérés.
Kakovost vode - Navodilo za identifikacijo in štetje vzorcev bentoških kremenastih alg rek in jezer
Standard EN 14407 določa metode za identifikacijo in štetje relativnih deležev kremenastih alg na pripravljenih objektnih steklih ter metode za interpretacijo, povezano z oceno kakovosti vode v rekah in jezerih. Primeren je za uporabo z indeksi in metodami ocenjevanja, ki temeljijo na relativni številčnosti taksonov. Metode za identifikacijo in štetje se lahko uporabijo tudi za študijo bentoških kremenastih alg v drugih habitatih, če so za interpretacijo podatkov uporabljene metode, primerne za te habitate.
General Information
Relations
Overview
EN 14407:2014 - Water quality: Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes (CEN) provides standardized methods for identifying and counting benthic diatom taxa on prepared slides and for interpreting those data in the context of water quality assessment. Applicable to rivers and lakes, the standard supports indices and assessment methods based on relative taxon abundance and can be adapted to other benthic habitats with appropriate interpretation. EN 14407:2014 supersedes EN 14407:2004 and aligns with monitoring needs driven by policies such as the Water Framework Directive.
Key topics and technical requirements
- Principle: Clean diatom frustules/valves are mounted in a high-refractive-index medium and identified/enumerated using high-power light microscopy; results are interpreted with indices or reference-condition comparisons.
- Apparatus: Recommended equipment includes a mechanical-stage light microscope with 100× oil-immersion objective (phase contrast or DIC recommended), eyepiece graticule (calibrated to a stage micrometer), immersion oil, floras/identification guides and imaging/photomicroscopy tools for documentation.
- Taxonomy and nomenclature: Define and document the taxonomic level (usually species) and nomenclature sources in advance to avoid confusion between parallel naming systems.
- Units for enumeration: Decide whether counts use valves or frustules (two valves = one frustule); document the convention for comparability.
- Sample size and counting strategy: Typical count sizes are 300–500 units, but this should be set according to study objectives. Enumeration approaches include field-by-field counts or linear traverses using a graticule; consistent rules for partial/overlapping fields are required.
- Broken/unidentifiable specimens: Establish a priori rules (e.g., include only if ~¾ present or central area visible) to handle fragmented material.
- Quality assurance, archiving and data interpretation: Guidance on QA procedures, archiving slides/samples and applying indices or reference-condition frameworks for ecological status assessment.
Applications and who uses it
- Environmental laboratories and biomonitoring teams performing diatom-based water quality assessments
- Regulatory agencies and consultants implementing the Water Framework Directive or lake/river monitoring programs
- Researchers studying eutrophication, acidification, habitat condition or long-term ecological change
Practical benefits include improved comparability of diatom data, reproducible enumeration methods, and robust input for indices that inform ecological status and management decisions.
Related standards and references
- Supersedes EN 14407:2004 (CEN).
- Definitions reference ISO-style instruments (e.g., stage micrometer per ISO 10934-1 terminology).
- Designed to be used alongside national floras, regional checklists and index-specific taxonomic conventions.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Kakovost vode - Navodilo za identifikacijo in štetje vzorcev bentoških kremenastih alg rek in jezerWasserbeschaffenheit - Anleitung zur Bestimmung und Zählung von benthischen Kieselalgen in Fließgewässern und SeenQualité de l'eau - Guide pour l'identification et le dénombrement des échantillons de diatomées benthiques de rivières et de lacsWater quality - Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 14407:2014SIST EN 14407:2014en,fr,de01-maj-2014SIST EN 14407:2014SLOVENSKI
STANDARDSIST EN 14407:20051DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 14407
March 2014 ICS 13.060.70 Supersedes EN 14407:2004English Version
Water quality - Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes
Qualité de l'eau - Guide pour l'identification et le dénombrement des échantillons de diatomées benthiques de rivières et de lacs
Wasserbeschaffenheit - Anleitung zur Bestimmung und Zählung von benthischen Kieselalgen in Fließgewässern und Seen This European Standard was approved by CEN on 20 December 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 14407:2014 ESIST EN 14407:2014
Foreword .3 Introduction .4 1 Scope .5 2 Terms and definitions .5 3 Principle .6 4 Apparatus .6 5 Determining analytical strategy .7 5.1 Determining taxonomic criteria for analysis .7 5.2 Determining units for enumeration .7 5.3 Determining sample size .7 5.4 Determining approach to enumeration.7 5.5 Treatment of broken and other unidentifiable diatoms .8 6 Analytical procedures .9 7 Archiving data, slides and samples . 10 8 Quality assurance . 10 9 Data interpretation . 10 9.1 Use of indices and scores . 10 9.2 Comparison with reference conditions . 10 Annex A (informative)
Example for quality assurance of diatom analyses from the UK . 11 Bibliography . 13
...
Frequently Asked Questions
SIST EN 14407:2014 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Water quality - Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes". This standard covers: This European Standard specifies methods for the identification and enumeration of relative proportions of diatom taxa on prepared slides and of data interpretation relevant to assessments of water quality in rivers and lakes. It is suitable for use with indices and assessment methods based on the relative abundance of taxa. The methods for identification and enumeration may also be applied to the study of benthic diatoms in other habitats provided that data interpretation methods appropriate to these habitats are used.
This European Standard specifies methods for the identification and enumeration of relative proportions of diatom taxa on prepared slides and of data interpretation relevant to assessments of water quality in rivers and lakes. It is suitable for use with indices and assessment methods based on the relative abundance of taxa. The methods for identification and enumeration may also be applied to the study of benthic diatoms in other habitats provided that data interpretation methods appropriate to these habitats are used.
SIST EN 14407:2014 is classified under the following ICS (International Classification for Standards) categories: 13.060.10 - Water of natural resources; 13.060.70 - Examination of biological properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN 14407:2014 has the following relationships with other standards: It is inter standard links to SIST EN 14407:2005. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN 14407:2014 is associated with the following European legislation: EU Directives/Regulations: 2000/60/EC, 2009-01-0312, EUR-PUB. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
You can purchase SIST EN 14407:2014 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.
Loading comments...