SIST-TS CEN/TS 17705:2023

Plant biostimulants - Determination of phosphonates

This document specifies a method for the extraction and determination of phosphonates (P-PO3) in plant biostimulants using ion chromatography and conductivity detection (IC-CD).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.

Pflanzen-Biostimulanzien - Bestimmung von Phosphonaten

Dieses Dokument legt ein Verfahren zur Extraktion und Bestimmung von Phosphonaten (P PO3) in Biostimulanzien für die pflanzliche Anwendung mit Ionenchromatographie und Leitfähigkeits-nachweis (IC CD) fest.
Dieses Dokument gilt auch für die Mischungen von Düngeprodukten, in denen die Biostimulanzien für die pflanzliche Anwendung der Hauptbestandteil der Mischung sind. Andernfalls gilt die Technische Spezifikation für den Hauptbestandteil der Mischung.

Biostimulants des végétaux - Dosage des phosphonates

Le présent document spécifie une méthode d’extraction et de dosage des phosphonates (P-PO3) dans les biostimulants des végétaux par chromatographie ionique et détection conductimétrique (IC-CD).
Le présent document est également applicable aux mélanges de fertilisants dans lesquels les biostimulants des végétaux représentent la majorité du mélange. Dans le cas contraire, c’est la Spécification technique correspondant au fertilisant majoritairement présent dans le mélange qui s’applique.

Rastlinski biostimulanti - Določevanje fosfonatov

Ta dokument določa metodo za ekstrakcijo in določevanje fosfonatov (P-PO3) v rastlinskih biostimulantih z ionsko kromatografijo in detekcijo na osnovi prevodnosti (IC-CD).
Dokument je uporaben tudi za mešanice sredstev za gnojenje, pri katerih so rastlinski biostimulanti glavni del mešanice. V nasprotnem primeru se uporablja tehnična specifikacija za glavni del mešanice.

General Information

• Status: Published
• Public Enquiry End Date: 30-Nov-2021
• Publication Date: 05-Jan-2023
• ICS: 07.080 - Biology. Botany. Zoology
• ICS: 65.080 - Fertilizers
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 22-Dec-2022
• Due Date: 26-Feb-2023
• Completion Date: 06-Jan-2023
• Directive: 2019/1009 - Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003
• Mandate: M/564 - Fertilising products in support of Regulation (EU) 2019/1009
• Mandate: M/XXX - Ambient Air Sensor systems and data quality objectives
• Ref Project: CEN/TS 17705:2022 - Plant biostimulants - Determination of phosphonates

Relations

• Revised: oSIST prEN 17705:2023 - Plant biostimulants - Determination of phosphonates
• Effective Date: 01-May-2023

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 17705:2023
01-februar-2023
Rastlinski biostimulanti - Določevanje fosfonatov
Plant biostimulants - Determination of phosphonates
Pflanzen-Biostimulanzien - Bestimmung von Phosphonaten
Biostimulants des végétaux - Dosage des phosphonates
Ta slovenski standard je istoveten z: CEN/TS 17705:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17705:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17705:2023

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SIST-TS CEN/TS 17705:2023


CEN/TS 17705
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

March 2022
TECHNISCHE SPEZIFIKATION
ICS 07.080
English Version

Plant biostimulants - Determination of phosphonates
Biostimulants des végétaux - Dosage des phosphonates Biostimulanzien für die pflanzliche Anwendung -
Bestimmung von Phosphonaten
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17705:2022 E
worldwide for CEN national Members.

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SIST-TS CEN/TS 17705:2023
CEN/TS 17705:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Procedure. 6
7.1 Sample preparation . 6
7.2 Water extraction. 6
7.3 Preparation of the calibration solutions . 7
7.4 Measurement . 7
8 Calculation and expression of the results . 9
8.1 Integration of peaks . 9
8.2 Concentration of phosphonates in test solutions . 9
9 Test report . 10
Annex A (informative) Separation of phosphonates from other interfering ions in a mixture
of standards . 11
Bibliography . 12

2

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SIST-TS CEN/TS 17705:2023
CEN/TS 17705:2022 (E)
European foreword
This document (CEN/TS 17705:2022) has been prepared by the Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
3

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SIST-TS CEN/TS 17705:2023
CEN/TS 17705:2022 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant Biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the market of
EU fertilizing products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as M/564, also contributes to the Communication on “Innovating
for Sustainable Growth: A Bio economy for Europe”. The Working Group 4 “Other safety parameters”, was
created to develop a work program as part of this request. The technical committee CEN/TC 455 “Plant
Biostimulants” was established to carry out the work program that will prepare a series of standards. The
interest in biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
The preparation of this document is based on the Fertilising Products Standardization Request (M/564)
to CEN by the European Commission and the European Free Trade Association concerning a development
of the methods for analysis of fertilizers in the framework of Regulation (EC) No 2019/1009.
This document describes the extraction and measurement for the determination of phosphonate
(phosphite) in plant biostimulants. It is based on a water extraction of the phosphonate (phosphite)
followed by ion chromatography with conductivity detection (IC-CD).
The ion chromatography with a conductivity detector (IC-CD) method can be used in well-equipped
analytical laboratories for the determination of different ions. In the field of fertilizing products, the
method is used and standardized for the determination of perchlorates in mineral fertilizers. The IC-CD
method can determine more ions simultaneously.
The legislative limit for phosphonate content is 0,5 % (mass fraction) and the method described in this
document was adapted to achieve this requirement and simultaneously to reduce interferences from
other co-extracted anions as much as possible.
The definition of phosphonates is not clearly stated in the Regulation 2019/1009 and to avoid any
misunderstanding the results are expressed as a content of phosphorus (P) bound in the form of free
water-soluble phosphonates (P-PO3).

4

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SIST-TS CEN/TS 17705:2023
CEN/TS 17705:2022 (E)
1 Scope
This document specifies a method for the extraction and determination of phosphonates (P–PO ) in plant
3
biostimulants using ion chromatography and conductivity detection (IC-CD).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the
main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
CEN/TS 17704, Plant biostimulants — Determination of dry matter
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
phosphonates
salts derived from phosphonic acid (H PO )
3 3
4 Principle
A representative test portion of the sample is extracted with water. Phosphonate in the extract is
selectively separated from other compounds using ion chromatography (IC) and determined by a
conductivity detector (CD). External calibration is used for quantification of the amount of the
phosphonate.
5 Reagents
All reagents shall be of recognized analytical grade. The concentration of phosphonate in the reagents
and deionized water used shall be low enough not to affect the results of the determination.
5.1 Water with a specific conductivity not higher than 0,2 mS/m at 25 °C.
5.2 Phosphonate standard stock solution r(P-PO3) = 1 000 mg/l is prepared by dissolving
commercially available sodium phosphite dibasic pentahydrate salt (Mw = 216,04 mol/l, purity ≥ 98 %).
For preparation the phosphonate standard stock solution 0,697 ± 0,001 g is weighed, transferred to
100 ml volumetric flask, fill with deionized water (5.1) to final volume and mix thoroughly.
5.3 Phosphonate standard solution, r(P-PO3) = 100 mg/l is prepared from a standard stock solution
(5.2) by appropriate dilution with deionized water (5.1). Pipette 10 ml of stock solution (5.2) to a 100ml
volumetric flask, fill with deionized water (5.1) to final volume and mix thoroughly.
5.4 Mobile phase, KOH cartridge for ion chromatography (commercially supplied). Concentration of
the KOH mobile phase is e
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17705:2021
01-november-2021
Rastlinski biostimulansi - Določevanje fosfonatov
Plant biostimulants - Determination of phosphonates
Biostimulanzien für die pflanzliche Anwendung - Bestimmung von Phosphonaten
Biostimulants des végétaux - Dosage des phosphonates
Ta slovenski standard je istoveten z: FprCEN/TS 17705
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17705:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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kSIST-TS FprCEN/TS 17705:2021

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kSIST-TS FprCEN/TS 17705:2021


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17705
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

September 2021
ICS 07.080
English Version

Plant biostimulants - Determination of phosphonates
Biostimulants des végétaux - Dosage des phosphonates Biostimulanzien für die pflanzliche Anwendung -
Bestimmung von Phosphonaten


This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 455.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17705:2021 E
worldwide for CEN national Members.

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kSIST-TS FprCEN/TS 17705:2021
FprCEN/TS 17705:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Procedure. 6
7.1 Sample preparation . 6
7.2 Water extraction. 6
7.3 Preparation of the calibration solutions . 7
7.4 Measurement . 7
7.4.1 Instrument conditions . 7
7.4.2 IC-CD measurement . 8
8 Calculation and expression of the results . 9
8.1 Integration of peaks . 9
8.2 Concentration of phosphonates in test solutions . 9
9 Test report . 10
Annex A (informative) Separation of phosphonates from other interfering ions in a mixture
of standards . 11
Bibliography . 12

2

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kSIST-TS FprCEN/TS 17705:2021
FprCEN/TS 17705:2021 (E)
European foreword
This document (FprCEN/TS 17705:2021) has been prepared by the Technical Committee CEN/TC 455
“Plant biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the Vote on TS.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association.

3

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kSIST-TS FprCEN/TS 17705:2021
FprCEN/TS 17705:2021 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 ‘Plant Biostimulants’. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the market of
EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This request, presented as SR M/564, also contributes to the Communication on “Innovating for
Sustainable Growth: A Bio economy for Europe”. The Working Group 4 “Other safety parameters”, was
created to develop a work program as part of this Request. The technical committee CEN/TC 455 ‘Plant
Biostimulants’ was established to carry out the work program that will prepare a series of standards. The
interest in biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
The preparation of this document is based on the Fertilising Products Standardization Request (M/564)
to CEN by the European Commission and the European Free Trade Association concerning a development
of the methods for analysis of fertilizers in the framework of Regulation (EC) No 2019/1009.
This document describes the extraction and measurement for the determination of phosphonate
(phosphite) in plant biostimulants. It is based on a water extraction of the phosphonate (phosphite)
followed by ion chromatography with conductivity detection (IC-CD).
The ion chromatography with a conductivity detector (IC-CD) method can be used in well-equipped
analytical laboratories for the determination of different ions. In the field of fertilizing products, the
method is used and standardized for the determination of perchlorates in mineral fertilizers. The IC-CD
method can determine more ions simultaneously.
The legislative limit for phosphonate content is 0,5 % (mass fraction) and the method described in this
document was adapted to achieve this requirement and simultaneously to reduce interferences from
other co-extracted anions as much as possible.
The definition of phosphonates is not clearly stated in the Regulation 2019/1009 and to avoid any
misunderstanding the results are expressed as a content of phosphorus (P) bound in the form of free
water-soluble phosphonates (P-PO3).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the
main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.

4

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kSIST-TS FprCEN/TS 17705:2021
FprCEN/TS 17705:2021 (E)
1 Scope
This document specifies a method for the extraction and determination of phosphonates (P–PO ) in plant
3
biostimulants using ion chromatography and conductivity detection (IC-CD).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the
main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
FprCEN/TS 17704:2021, Plant biostimulants — Determination of dry matter
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
• IEC Electropedia: available at https://www.electropedia.org/
• ISO Online browsing platform: available at https://www.iso.org/obp
3.1
phosphonates
salts derived from phosphonic acid (H PO )
3 3
4 Principle
A representative test portion of the sample is extracted with water. Phosphonate in the extract is
selectively separated from other compounds using ion chromatography (IC) and determined by a
conductivity detector (CD). External calibration is used for quantification of the amount of the
phosphonate.
5 Reagents
All reagents shall be of recognized analytical grade. The concentration of phosphonate in the reagents
and deionized water used shall be low enough not to affect the results of the determination.
5.1 Water with a specific conductivity not higher than 0,2 mS/m at 25 °C.
5.2 Phosphonate standard stock solution r(P-PO3) = 1 000 mg/l is prepared by dissolving
commercially available sodium phosphite dibasic pentahydrate salt (Mw = 216,04 mol/l, purity ≥ 98 %).
For preparation the phosphonate standard stock solution 0,697 ± 0,001 g is weighed, transferred to
100 ml volumetric flask, fill with deionized water (5.1) to final volume and mix thoroughly.
5.3 Phosphonate standard solution, r(P-PO3) = 100 mg/l is prepared from a standard stock solution
(5.2) by appropriate dilution with deionized water (5.1). Pipette 10 ml of stock solution (5.2) to a 100ml
volumetric flask, fill with deionized water (5.1) to final volume and mix thoroughly.
5.4 Mobile phase, KOH cartridge for ion chromatography (commercially supplied). Concentration of
the KOH mobile phase is electrolytically generated in externally supplied deionized water (5.1).
5

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kSIST-TS FprC
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