SIST EN 15196:2007
Water quality - Guidance on sampling and processing of the pupal exuviae of Chironomidae (Order Diptera) for ecological assessment
Water quality - Guidance on sampling and processing of the pupal exuviae of Chironomidae (Order Diptera) for ecological assessment
This guidance standard specifies equipment and procedures for collecting floating pupal exuviae of Chironomidae from aquatic habitats; rivers from source to estuary, canals, ponds, lakes and sea coasts. Guidance in preparing specimens for subsequent identification is provided. These samples provide representative data on relative species abundance, suitable for numerical analysis, classification and monitoring of environmental conditions.
Wasserbeschaffenheit - Anleitung zur Probenahme und Behandlung von Exuvien von Chironomidae-Larven (Diptera) zur ökologischen Untersuchung
Diese Europäische Norm legt die Geräte und Vorgehensweisen fest, nach denen flotierende Puppenexuvien
von Chironomiden aquatischer Habitate gesammelt werden; die aquatischen Habitate umfassen Flüsse von
der Quelle bis zur Mündung, Kanäle, Teiche, Seen und die Meeresküste. Hinweise zur Präparation der
Proben für die anschließende Bestimmung der Arten werden gegeben. Diese Proben dienen zur Ermittlung
repräsentativer Daten über relative Artenabundanzen, die für eine zahlenmäßige Erfassung, Klassifikation
sowie für die Überwachung der Umweltbedingungen geeignet sind.
Qualité de l'eau - Guide d'échantillonnage et de traitement d'exuvies nymphales de Chironomidae (Ordre des Dipteres) pour l'évaluation écologique
Le présent guide décrit l'équipement et les modes opératoires pour recueillir des exuvies nymphales de Chironomidae flottant a la surface des systemes aquatiques : rivieres depuis la source jusqu'a l'estuaire, canaux, étangs, lacs et littoraux marins. Il fournit les indications nécessaires a la préparation des spécimens pour leur identification ultérieure. Ces échantillons fournissent des données représentatives de l'abondance relative des especes, convenant pour l'analyse numérique, la classification et la surveillance des conditions environnementales.
Kakovost vode – Navodilo za vzorčenje in obdelavo levov bub trzač (Diptera: Chironomidae) za ekološko ocenjevanje
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Water quality - Guidance on sampling and processing of the pupal exuviae of Chironomidae (Order Diptera) for ecological assessmentQualité de l'eau - Guide d'échantillonnage et de traitement d'exuvies nymphales de Chironomidae (Ordre des Dipteres) pour l'évaluation écologiqueWasserbeschaffenheit - Anleitung zur Probenahme und Behandlung von Exuvien von Chironomidae-Larven (Diptera) zur ökologischen Untersuchung13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of waterICS:SIST EN 15196:2007enTa slovenski standard je istoveten z:EN 15196:200601-februar-2007SIST EN 15196:2007SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 15196August 2006ICS 13.060.45 English VersionWater quality - Guidance on sampling and processing of thepupal exuviae of Chironomidae (Order Diptera) for ecologicalassessmentQualité de l'eau - Guide d'échantillonnage et de traitementd'exuvies nymphales de Chironomidae (Ordre desDiptères) pour l'évaluation écologiqueWasserbeschaffenheit - Anleitung zur Probenahme undBehandlung von Exuvien von Chironomidae-Larven(Diptera) zur ökologischen UntersuchungThis European Standard was approved by CEN on 26 June 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2006 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 15196:2006: E
EN 15196:2006 (E) 2 Contents Page Foreword.3 1 Scope.4 2 Normative references.4 3 Terms and definitions.4 4 Principle.5 5 Sampling equipment.5 6 Sampling procedure.6 7 Quality assurance.7 Bibliography.8
EN 15196:2006 (E) 3 Foreword This document (EN 15196:2006) has been prepared by Technical Committee CEN/TC 230 “Water analysis”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by February 2007, and conflicting national standards shall be withdrawn at the latest by February 2007. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. WARNING — Working in or around water is inherently dangerous. Persons using this standard should be familiar with normal laboratory practice. This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate health and safety practices and to ensure compliance with any national regulatory guidelines.
EN 15196:2006 (E) 4 1 Scope This guidance standard specifies equipment and procedures for collecting floating pupal exuviae of Chironomidae from aquatic habitats; rivers from source to estuary, canals, ponds, lakes and sea coasts. Guidance in preparing specimens for subsequent identification is provided. These samples provide representative data on relative species abundance, suitable for numerical analysis, classification and monitoring of environmental conditions. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. No normative references. 3 Terms and definitions For the purpose of this European Standard, the following terms and definitions apply 3.1 adult terrestrial, reproductive phase of the life history 3.2 Chironomidae family of true flies (Diptera) known in the adult stage as non-biting midges 3.3 exuviae cast skin of an arthropod developing to a subsequent life stage NOTE From the Greek word meaning “cast clothes”, there is no singular term. 3.4 larva among aquatic species of Chironomidae this is the aquatic, juvenile phase of their life history 3.5 leeward shore lake edge to which the wind is blowing 3.6 pupa intermediate stage linking the larval and adult phases of a metamorphosing insect
EN 15196:2006 (E) 5 4 Principle A collection of floating chironomid pupal exuviae can be used for assessing and classifying most water bodies, without changing the equipment or procedure used (see Clause 6). The technique was first used by Thienemann in 1910 [12]. Chironomidae have exceptional species richness and ecological diversity, with an estimated 2 000 to 3 000 species per biogeographical region [3]. Pupae of all aquatic species of Chironomidae rise to the water surface for adult emergence [6]. Discarded pupal exuviae float on the water surface due to trapped air and a wax-layered cuticle. After about two days, bacterial attack of the wax layer causes the exuviae to sink [2], [7], [14]. Wind and water currents will cause floating exuviae to drift. In canals and rivers up to 10 m wide and with a mean velocity up to 50 cm sec-1, 90 % of pupal exuviae become trapped behind vegetation or artefacts within 100 m of where the adult emerged and none normally drift further than 500 m [7], [8], [14]. A sample of floating debris from a single point along a river or canal will therefore contain an integrated collection of pupal exuviae accumulated over the previous couple of days. These exuviae are associated with individuals, which have colonised available habitats a short distance upstream of the sampling point. In ponds and lakes, pupal exuviae are assumed to drift without hindrance until they reach the shore or sink. A collection of chironomid pupal exuviae from standing water will be representative of recent adult emergence over a wide expanse of water. Owing to the passive collection of discarded pupal exuviae there is little opportunity for the operator to influence the contents of the sample. In a comparison of a number of 10 minute collections of surface-floating chironomid exuviae with handnet samples of benthic (bottom-dwelling) larvae and pupae, twice as many species were obtained with exuviae, and these could be processed in one quarter to one third of the time needed to process benthic samples [4]. The sample will often contain exuviae from other insect groups and it is therefore important to distinguish them from Chironomidae. It may be possible to use these non-chironomid exuviae for water quality assessment but little is known on their reliability in representing species inhabiting the waterbody sampled. Mayfly exuviae break up rapidly in turbulent water while some caddisfly species leave their pupal exuviae in the larval case and this would bias any sample of floating exuviae. 5 Sampling equipment 5.1 Handnet, consisting of an extendable handle and frame supporting a net of mesh, sized no greater than 250 µm. Alt
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