SIST-TP CEN/TR 17245:2019
(Main)Water quality - Technical report for the routine sampling of benthic diatoms from rivers and lakes adapted for metabarcoding analyses
Water quality - Technical report for the routine sampling of benthic diatoms from rivers and lakes adapted for metabarcoding analyses
This technical report specifies a method for the field sampling of benthic diatoms which will be then analysed by subsequent metabarcoding techniques for ecological status and water quality assessments. Data produced by this method are suitable for production of taxonomical diatom lists.
Wasserbeschaffenheit - Technischer Bericht zur Routine-Probenahme von benthischen Diatomeen in Flüssen und Seen für Metabarcode-Analysen
Qualité de l'eau - Rapport technique concernant l'échantillonnage de routine de diatomées benthiques dans les rivières et les lacs adapté aux analyses reposant sur l'utilisation de métacodes à barres d'ADN
Le présent rapport technique spécifie une méthode d’échantillonnage sur le terrain de diatomées
benthiques qui seront ensuite analysées par des techniques ultérieures en metabarcoding afin d’évaluer
l’état écologique et la qualité de l’eau. Les données produites par cette méthode sont appropriées pour
produire des listes de diatomées taxinomiques.
Kakovost vode - Tehnično poročilo o rutinskem vzorčenju bentoških kremenastih alg v rekah in jezerih, prilagojeno za analize metabarkodiranja
To tehnično poročilo določa metodo za vzorčenje bentoških kremenastih alg na terenu, ki se nato analizirajo z naknadnimi tehnikami metabarkodiranja za ekološko stanje in oceno kakovosti vode. Podatki, pridobljeni s to metodo, so primerni za izdelavo taksonomskih seznamov kremenastih alg.
General Information
Overview
CEN/TR 17245:2018 - "Water quality - Technical report for the routine sampling of benthic diatoms from rivers and lakes adapted for metabarcoding analyses" provides field-level guidance for collecting benthic diatom samples optimized for downstream metabarcoding (environmental DNA) workflows. It supplements EN 13946:2014 by adapting routine sampling, equipment cleanliness and preservation steps so that DNA integrity is maintained for molecular taxonomic lists and ecological status assessments.
Key topics and requirements
- Scope: Field sampling methods for benthic diatoms from submerged hard substrata, macrophytes, littoral zones of lakes and rivers, with procedures adapted for metabarcoding analyses. Data are suitable for producing taxonomical diatom lists.
- Cleanliness and contamination control: Sample bottles and reagents must be sterile and free of DNA traces. Tools (e.g., toothbrushes, knives) should be clean and dedicated or DNA-free to avoid cross-contamination.
- Preservation methods (critical for environmental DNA):
- Ethanol: Add immediately in the field to reach a final concentration of 70% in the sample bottle.
- Deep-freezing: Store in a cooling box (~7 °C) in the field, and deep-freeze at −20 °C or below within 4–5 hours.
- Commercial DNA/RNA preservation solutions: Allowed if validated for routine use and used per manufacturer guidance.
- Not suitable: Lugol’s iodine and formaldehyde do not adequately preserve environmental DNA and are not recommended.
- Sampling methods: Follow EN 13946 procedures for site selection, choice of substratum, sampling movable hard surfaces, vertical man-made surfaces, artificial substrata, submerged macrophytes and emergent macrophytes - with added DNA-centric steps (immediate preservation, sterile labeling).
- Definitions: Clarifies terms such as barcode, base pair, and environmental DNA to align sampling with molecular analysis needs.
- Limitations: Metabarcoding laboratory protocols (PCR, sequencing, bioinformatics) are not included and will be provided in separate standards/technical reports.
Applications and users
- Environmental monitoring agencies conducting water quality and ecological status assessments under WFD and other directives.
- Environmental laboratories and biomonitoring practitioners implementing metabarcoding-based diatom surveys.
- Researchers developing molecular biomonitoring protocols or building diatom reference libraries.
- Consulting firms performing river and lake biomonitoring, restoration assessments, or baseline biodiversity surveys.
Related standards
- EN 13946:2014 - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes (CEN/TR 17245 is a supplement for metabarcoding-adapted sampling).
Keywords: water quality, benthic diatoms, metabarcoding, environmental DNA, field sampling, sample preservation, EN 13946, ecological status, biomonitoring.
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2019
Kakovost vode - Tehnično poročilo o rutinskem vzorčenju bentoških kremenastih
alg v rekah in jezerih, prilagojeno za analize metabarkodiranja
Water quality - Technical report for the routine sampling of benthic diatoms from rivers
and lakes adapted for metabarcoding analyses
Wasserbeschaffenheit - Technischer Bericht zur Routine-Probenahme von benthischen
Diatomeen in Flüssen und Seen für Metabarcode-Analysen
Qualité de l'eau - Rapport technique concernant l'échantillonnage de routine de
diatomées benthiques dans les rivières et les lacs adapté aux analyses reposant sur
l'utilisation de métacodes à barres d'ADN
Ta slovenski standard je istoveten z: CEN/TR 17245:2018
ICS:
13.060.10 Voda iz naravnih virov Water of natural resources
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
CEN/TR 17245
TECHNICAL REPORT
RAPPORT TECHNIQUE
September 2018
TECHNISCHER BERICHT
ICS 13.060.70
English Version
Water quality - Technical report for the routine sampling
of benthic diatoms from rivers and lakes adapted for
metabarcoding analyses
Qualité de l'eau - Rapport technique pour Wasserbeschaffenheit - Technischer Bericht zur
l'échantillonnage en routine de diatomées benthiques routinemäßigen Beprobung benthischer Diatomeen
dans les rivières et les plans d'eau adapté pour les aus Fließgewässern und Seen angepasst für
analyses en metabarcoding Metabarcodinganalysen
This Technical Report was approved by CEN on 14 May 2018. It has been drawn up by the Technical Committee CEN/TC 230.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 17245:2018 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
4.1 General . 5
4.2 Equipment for Field sampling . 5
5 Reagents . 6
5.1 General . 6
5.2 Preservatives . 6
5.2.1 General . 6
5.2.2 90 % Ethanol (C2H5OH) . 6
5.2.3 Deep-freezing . 6
5.2.4 Other commercial preservatives . 6
6 Procedure. 6
6.1 Choice of substratum . 6
6.2 Sample site selection . 6
6.3 Sampling methods . 6
6.3.1 Moveable natural hard surfaces . 6
6.3.2 Method for sampling vertical man-made surfaces in situ . 6
6.3.3 Use of introduced (“artificial”) substrata . 7
6.3.4 Sample collection from submerged macrophytes and macroalgae . 7
6.3.5 Sample collection from emergent macrophytes . 7
6.4 Sample preservation . 7
6.4.1 Ethanol preservation . 7
6.4.2 Deep-freezing preservation . 7
6.4.3 Other commercial preservatives . 7
Bibliography . 8
European foreword
This document (CEN/TR 17245:2018) has been prepared by Technical Committee CEN/TC 230 “Water
analysis”, the secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Introduction
WARNING — Persons using this document should be familiar with normal laboratory practice. This
technical report does not purport to address all of the safety problems, if any, associated with its use. It
is the responsibility of the user to establish appropriate health and safety practices and to ensure
compliance with any national regulatory conditions.
Diatoms are unicellular microalgae present in all types of water bodies. They are an important
component of aquatic ecosystems and have been used widely for ecological assessment required by the
Water Framework Directive (2000/60/EC) and Urban Waste Water Treatment Directive (91/217/EEC)
in addition to other EU Directives and international agreements. The use of diatoms as indicators of
river and lake quality is based on observations that diatom taxa have distinct preferences for particular
environmental conditions such as nutrients, organic pollution and acidity. Polluted waters will tend to
support higher proportions of those taxa whose optima correspond with the levels of the pollutant in
question. Conversely, certain species are intolerant of elevated levels of one or more pollutants, whilst
others may occur in a wide range of water qualities.
Methods using diatoms to assess water quality and ecological status based on optical microscopy have
been developed in several European countries. Methods for sampling and preparation are similar [6],
[7] leading to the development of European Standards which, in turn, facilitated the harmonization of
ecological assessment approaches [2], [3]. More r
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Frequently Asked Questions
SIST-TP CEN/TR 17245:2019 is a technical report published by the Slovenian Institute for Standardization (SIST). Its full title is "Water quality - Technical report for the routine sampling of benthic diatoms from rivers and lakes adapted for metabarcoding analyses". This standard covers: This technical report specifies a method for the field sampling of benthic diatoms which will be then analysed by subsequent metabarcoding techniques for ecological status and water quality assessments. Data produced by this method are suitable for production of taxonomical diatom lists.
This technical report specifies a method for the field sampling of benthic diatoms which will be then analysed by subsequent metabarcoding techniques for ecological status and water quality assessments. Data produced by this method are suitable for production of taxonomical diatom lists.
SIST-TP CEN/TR 17245:2019 is classified under the following ICS (International Classification for Standards) categories: 13.060.10 - Water of natural resources; 13.060.70 - Examination of biological properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.
You can purchase SIST-TP CEN/TR 17245:2019 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
SIST-TP CEN/TR 17245:2019 표준은 수생 생태계 평가와 수질 분석을 위한 벤치식 규조류의 채집 방법을 명확하게 규정하고 있습니다. 이 기술 보고서는 강과 호수에서 벤치식 규조류를 채집하기 위한 현장 샘플링 방법을 제시하며, 채집된 샘플은 메타바코딩 기술을 통해 분석됩니다. 이러한 접근방식은 생태적 상태와 수질 평가에 필수적인 데이터를 제공합니다. 이 표준의 주요 강점 중 하나는 생태적 다양성과 수질을 평가하기 위한 실용적인 방법론을 제공한다는 점입니다. 정량적 데이터 수집이 가능하여, 종적 규조 목록의 작성에 적합한 정보를 생산합니다. 이는 생물다양성 모니터링 및 수생태계 보호를 위한 기반 자료를 제공합니다. 또한, SIST-TP CEN/TR 17245:2019는 메타바코딩 기술에 대한 응용 가능성을 강조하여 최신 생물 분류학적 분석의 흐름에 부합합니다. 현장 샘플링 방식을 엄격히 규정함으로써, 일관된 데이터 수집이 이루어지며, 이는 연구 결과의 신뢰도를 높이는 데 기여합니다. 결론적으로, 벤치식 규조류의 샘플링을 위한 이 표준은 생태적 연구와 수질 평가에 매우 중요한 역할을 하며, 수생 생태계 모니터링의 필수적인 기준이 되고 있습니다.
SIST-TP CEN/TR 17245:2019は、水質に関する技術報告書であり、河川や湖から底生藻類（ベントス藻類）のルーチンサンプリングを行うための手法を規定しています。この標準は、エコロジカルステータスおよび水質の評価に利用されるメタバーニング解析に適用されるデータを生成することを目的としています。 本標準の範囲は、ベントス藻類のフィールドサンプリング手法を明確に定義しており、サンプリングプロセスが環境評価においてどのように役立つかを示しています。特に、取得されたデータは、分類学的な藻類リストの作成に非常に適しており、環境モニタリングや水質管理施策において重要な役割を果たします。これにより、研究者や環境管理者は、より正確な生態系の状況評価を行うことができるのです。 また、SIST-TP CEN/TR 17245:2019は、メタバーニング解析に基づく高度な技術との統合を反映しており、最新の科学的アプローチを取り入れています。このことは、環境科学の分野における研究の質を向上させ、持続可能な水資源管理への貢献を促進します。 この技術報告書は、底生藻類のサンプリングに関する標準化されたプロトコルを提供することで、今後の研究や実務において非常に重要なものであり、環境状態の評価手法の向上に大いに寄与するものであることが明確です。
The SIST-TP CEN/TR 17245:2019 standard provides a comprehensive framework for the routine sampling of benthic diatoms, specifically tailored for metabarcoding analyses in aquatic environments such as rivers and lakes. By outlining a systematic approach for field sampling, this technical report significantly contributes to the assessment of ecological status and water quality, which are critical factors in environmental monitoring and management. One of the primary strengths of this standard is its focus on ensuring the quality and consistency of data collected during the sampling process. The specified methodologies for sampling benthic diatoms enhance the reliability of data that can be interpreted for ecological evaluations. Additionally, the standard's adaptation for metabarcoding techniques reflects modern advancements in molecular biology, allowing for more accurate and efficient taxonomical diatom identification. This integration of cutting-edge technology positions the standard at the forefront of environmental monitoring practices. The relevance of SIST-TP CEN/TR 17245:2019 is underscored by its applicability in various ecological studies, particularly in the context of assessing water quality. The ability to produce taxonomical diatom lists from the sampling procedures outlined ensures that researchers and environmental regulators have access to robust and actionable data. Furthermore, the focus on benthic diatoms-a sensitive bioindicator of ecosystem health-highlights the standard's contribution to developing sustainable water management policies and strategies. In conclusion, the SIST-TP CEN/TR 17245:2019 standard stands out not only for its detailed procedural guidance but also for its critical role in enhancing our understanding of water quality and ecological dynamics through scientifically rigorous methods.
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